Reviewed, UniProtKB/Swiss-Prot Q44274 (GYRB_ACIGB)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit B
    EC=5.99.1.3

Gene names

Name:

gyrB

Organism

Acinetobacter genomosp. 11

Taxonomic identifier

106649 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	389 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the type II topoisomerase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›389	›389	DNA gyrase subunit B		PRO_0000145284
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	389	1

Sequences

	Sequence		Length	Mass (Da)
	Q44274-1 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 164E4F2B527F29F5	FASTA	389	43,035
	10 20 30 40 50 60 DNSYKVSGGL HGVGVSVVNA LSSKLELTIH RAGHIHQQEY KHGDPVYPLK IVGDVETTGT 70 80 90 100 110 120 IVRFWPSTET FSQTIFNVDI LARRLRELSF LNAGVRIVLR DERVALEHVF DYEGGLSEFV 130 140 150 160 170 180 KYINQGKTHL NEIFHFTTQA ENGIGVEVAL QWNDTYQENV RCFTNNIPQK DGGTHLAGFR 190 200 210 220 230 240 AALTRGLNSY MENENLLKKE KVAVTGDDAR EGLTAIVSVK VPDPKFSSQT KEKLVSSEVK 250 260 270 280 290 300 PAVEQAMNKA FSEYLLENPQ AAKSIAGKII DAARARDAAR KAREMTRRKS ALDIAGLPGK 310 320 330 340 350 360 LADCQEKDPA LSELYLVEGD SAGGSAKQGR NRKMQAILPL KGKILNVERA RFDKMISSQE 370 380 VGTLITALGC GIGREEYNPD KLRYHKIII

References

[1]	"Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization." Yamamoto S., Bouvet P.J.M., Harayama S. Int. J. Syst. Bacteriol. 49:87-95(1999) [PubMed: 10028249] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11171 / NCIB 8250 / CIP 63.46 / B94.
[2]	"Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products." Yamamoto S., Harayama S. Int. J. Syst. Bacteriol. 46:506-511(1996) [PubMed: 8934907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-118 AND 289-388. Strain: ATCC 11171 / NCIB 8250 / CIP 63.46 / B94.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB008690 Genomic DNA. Translation: BAA75407.1. D73437 Genomic DNA. Translation: BAA11162.1. D73422 Genomic DNA. Translation: BAA11147.1.
3D structure databases
SMR	Q44274. Positions 1-286, 294-389.
ModBase	Search...
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA_B/N. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF00204. DNA_gyraseB. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00433. TOP2c. 1 hit. [Graphical view]
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRB_ACIGB

Accession

Primary (citable) accession number: Q44274
Secondary accession number(s): Q60168, Q9ZA04

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 11, 2001
Last modified:	February 9, 2010
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)