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Protein

o-succinylbenzoate synthase

Gene

Aaar

Organism
Amycolatopsis sp.
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Converts 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) to 2-succinylbenzoate (OSB).UniRule annotationSAAS annotation

Catalytic activityi

(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H2O.UniRule annotationSAAS annotation

Cofactori

a divalent metal cationUniRule annotation

Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotationSAAS annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. o-succinylbenzoate synthase (Aaar)
  5. no protein annotated in this organism
  6. no protein annotated in this organism
  7. no protein annotated in this organism
This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

Pathwayi: menaquinone biosynthesis

This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161Proton donorUniRule annotation1
Metal bindingi189MagnesiumUniRule annotationCombined sources1
Metal bindingi191MagnesiumCombined sources1
Metal bindingi214MagnesiumUniRule annotationCombined sources1
Metal bindingi239MagnesiumUniRule annotationCombined sources1
Active sitei263Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationSAAS annotation

Keywords - Biological processi

Menaquinone biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

MagnesiumUniRule annotationCombined sourcesSAAS annotation, Metal-bindingUniRule annotationCombined sourcesSAAS annotation

Enzyme and pathway databases

SABIO-RKQ44244.
UniPathwayiUPA00079.
UPA01057; UER00165.

Names & Taxonomyi

Protein namesi
Recommended name:
o-succinylbenzoate synthaseUniRule annotationSAAS annotation (EC:4.2.1.113UniRule annotationSAAS annotation)
Short name:
OSB synthaseUniRule annotation
Short name:
OSBSUniRule annotation
Alternative name(s):
4-(2'-carboxyphenyl)-4-oxybutyric acid synthaseUniRule annotation
o-succinylbenzoic acid synthaseUniRule annotation
Gene namesi
Name:AaarImported
Synonyms:menCUniRule annotation
OrganismiAmycolatopsis sp.Imported
Taxonomic identifieri37632 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeAmycolatopsis

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SJAX-ray2.30A/B/C/D1-368[»]
1SJBX-ray2.20A/B/C/D1-368[»]
1SJCX-ray2.10A/B/C/D1-368[»]
1SJDX-ray1.87A/B/C/D1-368[»]
4A6GX-ray2.71A/B/C/D1-368[»]
ProteinModelPortaliQ44244.
SMRiQ44244.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ44244.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini142 – 235MR_MLEInterPro annotationAdd BLAST94

Sequence similaritiesi

Belongs to the mandelate racemase/muconate lactonizing enzyme family. MenC type 2 subfamily.UniRule annotation

Family and domain databases

CDDicd03317. NAAAR. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_01933. MenC_2. 1 hit.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
IPR010197. OSB_synthase_MenC_2.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PTHR13794:SF8. PTHR13794:SF8. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01928. menC_lowGC/arch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q44244-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSGVELRR VQMPLVAPFR TSFGTQSVRE LLLLRAVTPA GEGWGECVTM
60 70 80 90 100
AGPLYSSEYN DGAEHVLRHY LIPALLAAED ITAAKVTPLL AKFKGHRMAK
110 120 130 140 150
GALEMAVLDA ELRAHERSFA AELGSVRDSV PCGVSVGIMD TIPQLLDVVG
160 170 180 190 200
GYLDEGYVRI KLKIEPGWDV EPVRAVRERF GDDVLLQVDA NTAYTLGDAP
210 220 230 240 250
QLARLDPFGL LLIEQPLEEE DVLGHAELAR RIQTPICLDE SIVSARAAAD
260 270 280 290 300
AIKLGAVQIV NIKPGRVGGY LEARRVHDVC AAHGIPVWCG GMIETGLGRA
310 320 330 340 350
ANVALASLPN FTLPGDTSAS DRFYKTDITE PFVLSGGHLP VPTGPGLGVA
360
PIPELLDEVT TAKVWIGS
Length:368
Mass (Da):39,406
Last modified:November 1, 1996 - v1
Checksum:i623A27548DA0E8C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30738 Genomic DNA. Translation: BAA06400.1.
PIRiI39598.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30738 Genomic DNA. Translation: BAA06400.1.
PIRiI39598.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SJAX-ray2.30A/B/C/D1-368[»]
1SJBX-ray2.20A/B/C/D1-368[»]
1SJCX-ray2.10A/B/C/D1-368[»]
1SJDX-ray1.87A/B/C/D1-368[»]
4A6GX-ray2.71A/B/C/D1-368[»]
ProteinModelPortaliQ44244.
SMRiQ44244.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00165.
SABIO-RKQ44244.

Miscellaneous databases

EvolutionaryTraceiQ44244.

Family and domain databases

CDDicd03317. NAAAR. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_01933. MenC_2. 1 hit.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
IPR010197. OSB_synthase_MenC_2.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PTHR13794:SF8. PTHR13794:SF8. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01928. menC_lowGC/arch. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQ44244_9PSEU
AccessioniPrimary (citable) accession number: Q44244
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.