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Q44244

- Q44244_9PSEU

UniProt

Q44244 - Q44244_9PSEU

Protein
Submitted name:

N-acylamino acid racemase

Gene

Aaar

Organism
Amycolatopsis sp.
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi189 – 1891MagnesiumImported
    Metal bindingi191 – 1911Magnesium
    Metal bindingi214 – 2141MagnesiumImported
    Metal bindingi239 – 2391MagnesiumImported

    GO - Molecular functioni

    1. catalytic activity Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Ligandi

    MagnesiumImported, Metal-bindingSAAS annotationImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    N-acylamino acid racemaseImported
    Gene namesi
    Name:AaarImported
    OrganismiAmycolatopsis sp.Imported
    Taxonomic identifieri37632 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeAmycolatopsis

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SJAX-ray2.30A/B/C/D1-368[»]
    1SJBX-ray2.20A/B/C/D1-368[»]
    1SJCX-ray2.10A/B/C/D1-368[»]
    1SJDX-ray1.87A/B/C/D1-368[»]
    4A6GX-ray2.71A/B/C/D1-368[»]
    ProteinModelPortaliQ44244.
    SMRiQ44244. Positions 1-368.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ44244.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProiIPR029065. Enolase_C-like.
    IPR029017. Enolase_N_like.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N.
    IPR001354. MR_MLE.
    IPR010197. OSB_synthase_lowGC-bac-typ.
    [Graphical view]
    PANTHERiPTHR13794. PTHR13794. 1 hit.
    PTHR13794:SF8. PTHR13794:SF8. 1 hit.
    PfamiPF01188. MR_MLE. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view]
    SMARTiSM00922. MR_MLE. 1 hit.
    [Graphical view]
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsiTIGR01928. menC_lowGC/arch. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q44244-1 [UniParc]FASTAAdd to Basket

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    MKLSGVELRR VQMPLVAPFR TSFGTQSVRE LLLLRAVTPA GEGWGECVTM    50
    AGPLYSSEYN DGAEHVLRHY LIPALLAAED ITAAKVTPLL AKFKGHRMAK 100
    GALEMAVLDA ELRAHERSFA AELGSVRDSV PCGVSVGIMD TIPQLLDVVG 150
    GYLDEGYVRI KLKIEPGWDV EPVRAVRERF GDDVLLQVDA NTAYTLGDAP 200
    QLARLDPFGL LLIEQPLEEE DVLGHAELAR RIQTPICLDE SIVSARAAAD 250
    AIKLGAVQIV NIKPGRVGGY LEARRVHDVC AAHGIPVWCG GMIETGLGRA 300
    ANVALASLPN FTLPGDTSAS DRFYKTDITE PFVLSGGHLP VPTGPGLGVA 350
    PIPELLDEVT TAKVWIGS 368
    Length:368
    Mass (Da):39,406
    Last modified:November 1, 1996 - v1
    Checksum:i623A27548DA0E8C4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30738 Genomic DNA. Translation: BAA06400.1.
    PIRiI39598.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30738 Genomic DNA. Translation: BAA06400.1 .
    PIRi I39598.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SJA X-ray 2.30 A/B/C/D 1-368 [» ]
    1SJB X-ray 2.20 A/B/C/D 1-368 [» ]
    1SJC X-ray 2.10 A/B/C/D 1-368 [» ]
    1SJD X-ray 1.87 A/B/C/D 1-368 [» ]
    4A6G X-ray 2.71 A/B/C/D 1-368 [» ]
    ProteinModelPortali Q44244.
    SMRi Q44244. Positions 1-368.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q44244.

    Family and domain databases

    Gene3Di 3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProi IPR029065. Enolase_C-like.
    IPR029017. Enolase_N_like.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N.
    IPR001354. MR_MLE.
    IPR010197. OSB_synthase_lowGC-bac-typ.
    [Graphical view ]
    PANTHERi PTHR13794. PTHR13794. 1 hit.
    PTHR13794:SF8. PTHR13794:SF8. 1 hit.
    Pfami PF01188. MR_MLE. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view ]
    SMARTi SM00922. MR_MLE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsi TIGR01928. menC_lowGC/arch. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, DNA sequencing and heterologous expression of the gene for thermostable N-acylamino acid racemase from Amycolatopsis sp. TS-1-60 in Escherichia coli."
      Tokuyama S., Hatano K.
      Appl. Microbiol. Biotechnol. 42:884-889(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: TS-1-60Imported.
    2. "Evolution of enzymatic activity in the enolase superfamily: structural studies of the promiscuous o-succinylbenzoate synthase from Amycolatopsis."
      Thoden J.B., Taylor Ringia E.A., Garrett J.B., Gerlt J.A., Holden H.M., Rayment I.
      Biochemistry 43:5716-5727(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.87 ANGSTROMS) IN COMPLEX WITH MAGNESIUM.
    3. "An improved racemase/acylase biotransformation for the preparation of enantiomerically pure amino acids."
      Baxter S., Royer S., Grogan G., Brown F., Holt-Tiffin K.E., Taylor I.N., Fotheringham I.G., Campopiano D.J.
      J. Am. Chem. Soc. 134:19310-19313(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.71 ANGSTROMS) IN COMPLEX WITH MAGNESIUM.

    Entry informationi

    Entry nameiQ44244_9PSEU
    AccessioniPrimary (citable) accession number: Q44244
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 1, 1996
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3