N-acylamino acid racemase - Amycolatopsis sp.

Preferred order of sections

Names and origin

Protein names

Submitted name:
N-acylamino acid racemase EMBL BAA06400.1

Gene names

Name:

Aaar EMBL BAA06400.1

Organism

Amycolatopsis sp. EMBL BAA06400.1

Taxonomic identifier

37632 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Magnesium PDB 1SJA PDB 1SJB PDB 1SJC Metal-binding SAAS SAAS013342 PDB 1SJA PDB 1SJB PDB 1SJC
Technical term	3D-structure PDB 1SJA PDB 1SJD PDB 1SJB PDB 1SJC PDB 4A6G
Gene Ontology (GO)
Molecular_function	catalytic activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

189

1

Magnesium PDB 1SJA PDB 1SJB PDB 1SJC

Metal binding

191

1

Magnesium PDB 1SJB

Metal binding

214

1

Magnesium PDB 1SJA PDB 1SJB PDB 1SJC

Metal binding

239

1

Magnesium PDB 1SJA PDB 1SJB PDB 1SJC

Sequences

Sequence

Length

Mass (Da)

Tools

Q44244 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 623A27548DA0E8C4
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FASTA

368

39,406

        10         20         30         40         50         60 
MKLSGVELRR VQMPLVAPFR TSFGTQSVRE LLLLRAVTPA GEGWGECVTM AGPLYSSEYN 

        70         80         90        100        110        120 
DGAEHVLRHY LIPALLAAED ITAAKVTPLL AKFKGHRMAK GALEMAVLDA ELRAHERSFA 

       130        140        150        160        170        180 
AELGSVRDSV PCGVSVGIMD TIPQLLDVVG GYLDEGYVRI KLKIEPGWDV EPVRAVRERF 

       190        200        210        220        230        240 
GDDVLLQVDA NTAYTLGDAP QLARLDPFGL LLIEQPLEEE DVLGHAELAR RIQTPICLDE 

       250        260        270        280        290        300 
SIVSARAAAD AIKLGAVQIV NIKPGRVGGY LEARRVHDVC AAHGIPVWCG GMIETGLGRA 

       310        320        330        340        350        360 
ANVALASLPN FTLPGDTSAS DRFYKTDITE PFVLSGGHLP VPTGPGLGVA PIPELLDEVT 


TAKVWIGS

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References

[1]	"Cloning, DNA sequencing and heterologous expression of the gene for thermostable N-acylamino acid racemase from Amycolatopsis sp. TS-1-60 in Escherichia coli." Tokuyama S., Hatano K. Appl. Microbiol. Biotechnol. 42:884-889(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: TS-1-60 EMBL BAA06400.1.
[2]	"Evolution of enzymatic activity in the enolase superfamily: structural studies of the promiscuous o-succinylbenzoate synthase from Amycolatopsis." Thoden J.B., Taylor Ringia E.A., Garrett J.B., Gerlt J.A., Holden H.M., Rayment I. Biochemistry 43:5716-5727(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.87 ANGSTROMS) IN COMPLEX WITH MAGNESIUM.
[3]	"An improved racemase/acylase biotransformation for the preparation of enantiomerically pure amino acids." Baxter S., Royer S., Grogan G., Brown F., Holt-Tiffin K.E., Taylor I.N., Fotheringham I.G., Campopiano D.J. J. Am. Chem. Soc. 134:19310-19313(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.71 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D30738 Genomic DNA. Translation: BAA06400.1.

PIR

I39598.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SJA	X-ray	2.30	A/B/C/D	1-368	[»]
1SJB	X-ray	2.20	A/B/C/D	1-368	[»]
1SJC	X-ray	2.10	A/B/C/D	1-368	[»]
1SJD	X-ray	1.87	A/B/C/D	1-368	[»]
4A6G	X-ray	2.71	A/B/C/D	1-368	[»]

ProteinModelPortal

Q44244.

SMR

Q44244. Positions 1-368.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
IPR010197. OSB_synthase_lowGC-bac/arc.
[Graphical view]

PANTHER

PTHR13794. PTHR13794. 1 hit.
PTHR13794:SF8. PTHR13794:SF8. 1 hit.

Pfam

PF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]

SMART

SM00922. MR_MLE. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01928. menC_lowGC/arch. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q44244.

Entry information

Entry name

Q44244_9PSEU

Accession

Primary (citable) accession number: Q44244

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)