Reviewed, UniProtKB/Swiss-Prot Q44241 (NDHJ_ANASP)

Names and origin

Protein names

Recommended name:
    NAD(P)H-quinone oxidoreductase subunit J
    EC=1.6.5.-
Alternative name(s):
    NAD(P)H dehydrogenase subunit J
    NADH-plastoquinone oxidoreductase subunit J
    NDH-1 subunit J
      Short name=NDH-J

Gene names

Name:	ndhJ
Ordered Locus Names:	all3840

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient. Cyanobacterial NDH-1 also plays a role in inorganic carbon-concentration By similarity.
Catalytic activity	NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol. HAMAP MF_01357
Subunit structure	NDH-1 can be composed of about 15 different subunits; different subcomplexes with different compositions have been identified which probably have different functions By similarity.
Subcellular location	Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the complex I 30 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Thylakoid
Ligand	NAD NADP Plastoquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 175	175	NAD(P)H-quinone oxidoreductase subunit J HAMAP MF_01357		PRO_0000118665
Experimental info
	Sequence conflict	7	1	Q → K Ref.3
	Sequence conflict	13 – 14	2	EA → AE Ref.3
	Sequence conflict	50	1	A → P in AAC44354. Ref.3
	Sequence conflict	53	1	L → M in AAC44354. Ref.3
	Sequence conflict	74	1	V → I in AAC44354. Ref.3
	Sequence conflict	101	1	I → V in AAC44354. Ref.3
	Sequence conflict	152	1	E → G in AAC44354. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q44241-1 [UniParc]. Last modified January 23, 2002. Version 3. Checksum: FA257DBD9F76D3A4	FASTA	175	19,893
	10 20 30 40 50 60 MADEELQPVP AAEAAIVPSG PTSQWLTENG FAHESLAADK NGVEIIKVEA DFLLPIATAL 70 80 90 100 110 120 YAYGFNYLQF QGGVDLGPGQ DLVSVYHLVK VSDNADKPEE IRVKVFLPRE NPVVPSVYWI 130 140 150 160 170 WKTADWQERE SYDMFGIIYE GHPNLKRILM PEDWVGWPLR KDYISPDFYE LQDAY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterisation of the ndhCKJ-cluster of the cyanobacteria Anabaena sp. PCC 7120." Happe T., Schiefer W., Boehme H. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Cloning, analysis and inactivation of the ndhK gene encoding a subunit of NADH quinone oxidoreductase from Anabaena PCC 7120." Howitt C.A., Whelan J., Price G.D., Day D.A. Eur. J. Biochem. 240:173-180(1996) [PubMed: 8797851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-155.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ012180 Genomic DNA. Translation: CAB45641.1. BA000019 Genomic DNA. Translation: BAB75539.1. U31208 Genomic DNA. Translation: AAC44354.1. Different initiation.
PIR	AI2285. S74217.
RefSeq	NP_487880.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1107438.
GenomeReviews	Gene locus all3840 in contig BA000019_GR.
KEGG	ana:all3840.
NMPDR	fig|103690.1.peg.4147.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q44241.
OMA	Q44241. YELQDAY.
Enzyme and pathway databases
BioCyc	NSP103690:ALL3840-MON.
Family and domain databases
HAMAP	MF_01357. [Tree]
InterPro	IPR001268. NADH_UbQ_OxRdtase_30kDa_su. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00542. COMPLEX1_30K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDHJ_ANASP

Accession

Primary (citable) accession number: Q44241
Secondary accession number(s): Q9WWN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2002
Last modified:	June 16, 2009
This is version 55 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)