Q44233 (BGAL1_ARTSP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-galactosidase Short name=Beta-gal EC=3.2.1.23
Organism	Arthrobacter sp.
Taxonomic identifier	1667 [NCBI]
Taxonomic lineage	Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Highly specific towards beta-D-galactoside substrates. Hydrolyzes 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and o-nitrophenyl-beta-D-galactopyranoside (ONPG). Has activity against p-nitrophenyl(pNP)-beta-D-galactoside, but not significantly at all towards pNP-alpha-D-galactoside, pNP-beta-D-glucoside, pNP-beta-D-mannoside, pNP-beta-L-fucoside, pNP-beta-D-xyloside, pNP-beta-L-arabinoside, pNP-beta-D-galuronide, pNP-beta-D-glucuronide, pNP-beta-D-lactoside or pNP-beta-D-cellobioside. Ref.1
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Ref.1
Enzyme regulation	Activity stimulated by beta-mercaptoethanol. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 42 family.
Biophysicochemical properties	Kinetic parameters: KM=0.57 mM for ONPG Ref.1 KM=4.81 mM for lactose Vmax=254 µmol/min/mg enzyme with ONPG as substrate Vmax=3.97 µmol/min/mg enzyme with lactose as substrate pH dependence: Optimum pH is 6.6. Maintains activity over a broad range of pH values from 6 to 9. Temperature dependence: Optimum temperature is 45-50 degrees Celsius. Activity declines rapidly above 50 degrees Celsius. Stable for at least 70 hours at temperatures 35 degrees Celsius and below. At 50 degrees Celsius loses all activity in less than 15 minutes.
Sequence caution	The sequence AAA75601.1 differs from that shown. Reason: Frameshift at positions 111, 158 and 637.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 690	690	Beta-galactosidase		PRO_0000407680
Sites
	Active site	174	1	Proton donor By similarity
	Binding site	173	1	Substrate By similarity
	Binding site	345	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q44233 [UniParc]. Last modified May 3, 2011. Version 2. Checksum: 83262445ECE19128	FASTA	690	76,115
	10 20 30 40 50 60 MPVPTPLSEG TTPDTAAQEL RTNRLWEALP GLSYGGDYIP NSGRNRSARK IYRSCRKPEC 70 80 90 100 110 120 RPSALASSPG LGLEPVEGSY DFTWLDEVMD NLAATGIKVA LATATAAPPA GWLRKHPEIL 130 140 150 160 170 180 PVTAEGSTLG PARAHYLVVG MVLFCRPVCG EDDPRLGERY KDHPALALWH VDNELGCHVS 190 200 210 220 230 240 EFYGPRRHRR FPSMAEPTLR HDRGPQRGLG TAFWSQRYSC FEEILTPRPA PTTLNPTQQL 250 260 270 280 290 300 DFQRFSSWGL IDFYSMLARG HFARSHPRCP PRQIWWPQAP PCLWDYFDWA KKLECHRQWS 310 320 330 340 350 360 LPGGRRYRCV TSELAFRRRS DSEAIAGGKP WSPDGALSPC RPCNWLASQH DSRTPGEMAR 370 380 390 400 410 420 NSLVHVGRGI WMLSCFSSGD RASRVRRNST RPWCRTPEPT REYGVKLLSW AQAQSLVRGS 430 440 450 460 470 480 RRRGGITHRN RLRLRTLVGK RTGLHPAPMW KYLELLRAFH APCSCPASPP IWSIPALTLT 490 500 510 520 530 540 AMTWWSSRPC TPSPMPRPAI LRQRQNAEPQ CSSATSVDID ENDAVRLGGY PGAFRDLLGV 550 560 570 580 590 600 NVEEFHPLPE NSTVSLDAGW SGRIWSEHVH LTGAEAKVSF TEAPLTGVPA VTRHAVGTGA 610 620 630 640 650 660 AWYLATFPDA TGLESLLDSL IAESGVRAPA MAAAGVELSR RSHADGRSYL FAINHNVTEA 670 680 690 AVSAQGTELI SGTPFNGTVP AGAVAVIAEG

References

[1]

"Analysis of a novel gene and beta-galactosidase isozyme from a psychrotrophic Arthrobacter isolate." Gutshall K.R., Trimbur D.E., Kasmir J.J., Brenchley J.E. J. Bacteriol. 177:1981-1988(1995) [PubMed: 7721689] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 9-16, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY. Strain: B7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17417 Genomic DNA. Translation: AAA75601.1. Frameshift.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH42. Glycoside Hydrolase Family 42.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013739. Beta_galactosidase_C. IPR013738. Beta_galactosidase_Trimer. IPR003476. Glyco_hydro_42. IPR013529. Glyco_hydro_42_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02449. Glyco_hydro_42. 1 hit. PF08533. Glyco_hydro_42C. 1 hit. PF08532. Glyco_hydro_42M. 1 hit. [Graphical view]
PIRSF	PIRSF001084. B-galactosidase. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BGAL1_ARTSP

Accession

Primary (citable) accession number: Q44233

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	May 3, 2011
Last modified:	July 27, 2011
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program