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Q44233

- BGAL1_ARTSP

UniProt

Q44233 - BGAL1_ARTSP

Protein

Beta-galactosidase

Gene
N/A
Organism
Arthrobacter sp.
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 2 (03 May 2011)
      Previous versions | rss
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    Functioni

    Highly specific towards beta-D-galactoside substrates. Hydrolyzes 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and o-nitrophenyl-beta-D-galactopyranoside (ONPG). Has activity against p-nitrophenyl(pNP)-beta-D-galactoside, but not significantly at all towards pNP-alpha-D-galactoside, pNP-beta-D-glucoside, pNP-beta-D-mannoside, pNP-beta-L-fucoside, pNP-beta-D-xyloside, pNP-beta-L-arabinoside, pNP-beta-D-galuronide, pNP-beta-D-glucuronide, pNP-beta-D-lactoside or pNP-beta-D-cellobioside.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

    Enzyme regulationi

    Activity stimulated by beta-mercaptoethanol.1 Publication

    Kineticsi

    1. KM=0.57 mM for ONPG1 Publication
    2. KM=4.81 mM for lactose1 Publication

    Vmax=254 µmol/min/mg enzyme with ONPG as substrate1 Publication

    Vmax=3.97 µmol/min/mg enzyme with lactose as substrate1 Publication

    pH dependencei

    Optimum pH is 6.6. Maintains activity over a broad range of pH values from 6 to 9.1 Publication

    Temperature dependencei

    Optimum temperature is 45-50 degrees Celsius. Activity declines rapidly above 50 degrees Celsius. Stable for at least 70 hours at temperatures 35 degrees Celsius and below. At 50 degrees Celsius loses all activity in less than 15 minutes.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei173 – 1731SubstrateBy similarity
    Active sitei174 – 1741Proton donorBy similarity
    Binding sitei345 – 3451SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. galactose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    OrganismiArthrobacter sp.
    Taxonomic identifieri1667 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 690690Beta-galactosidasePRO_0000407680Add
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q44233-1 [UniParc]FASTAAdd to Basket

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    MPVPTPLSEG TTPDTAAQEL RTNRLWEALP GLSYGGDYIP NSGRNRSARK    50
    IYRSCRKPEC RPSALASSPG LGLEPVEGSY DFTWLDEVMD NLAATGIKVA 100
    LATATAAPPA GWLRKHPEIL PVTAEGSTLG PARAHYLVVG MVLFCRPVCG 150
    EDDPRLGERY KDHPALALWH VDNELGCHVS EFYGPRRHRR FPSMAEPTLR 200
    HDRGPQRGLG TAFWSQRYSC FEEILTPRPA PTTLNPTQQL DFQRFSSWGL 250
    IDFYSMLARG HFARSHPRCP PRQIWWPQAP PCLWDYFDWA KKLECHRQWS 300
    LPGGRRYRCV TSELAFRRRS DSEAIAGGKP WSPDGALSPC RPCNWLASQH 350
    DSRTPGEMAR NSLVHVGRGI WMLSCFSSGD RASRVRRNST RPWCRTPEPT 400
    REYGVKLLSW AQAQSLVRGS RRRGGITHRN RLRLRTLVGK RTGLHPAPMW 450
    KYLELLRAFH APCSCPASPP IWSIPALTLT AMTWWSSRPC TPSPMPRPAI 500
    LRQRQNAEPQ CSSATSVDID ENDAVRLGGY PGAFRDLLGV NVEEFHPLPE 550
    NSTVSLDAGW SGRIWSEHVH LTGAEAKVSF TEAPLTGVPA VTRHAVGTGA 600
    AWYLATFPDA TGLESLLDSL IAESGVRAPA MAAAGVELSR RSHADGRSYL 650
    FAINHNVTEA AVSAQGTELI SGTPFNGTVP AGAVAVIAEG 690
    Length:690
    Mass (Da):76,115
    Last modified:May 3, 2011 - v2
    Checksum:i83262445ECE19128
    GO

    Sequence cautioni

    The sequence AAA75601.1 differs from that shown. Reason: Frameshift at positions 111, 158 and 637.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17417 Genomic DNA. Translation: AAA75601.1. Frameshift.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17417 Genomic DNA. Translation: AAA75601.1 . Frameshift.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH42. Glycoside Hydrolase Family 42.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProi IPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001084. B-galactosidase. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a novel gene and beta-galactosidase isozyme from a psychrotrophic Arthrobacter isolate."
      Gutshall K.R., Trimbur D.E., Kasmir J.J., Brenchley J.E.
      J. Bacteriol. 177:1981-1988(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 9-16, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
      Strain: B71 Publication.

    Entry informationi

    Entry nameiBGAL1_ARTSP
    AccessioniPrimary (citable) accession number: Q44233
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 51 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3