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Q44233 (BGAL1_ARTSP) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
OrganismArthrobacter sp.
Taxonomic identifier1667 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Protein attributes

Sequence length690 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Highly specific towards beta-D-galactoside substrates. Hydrolyzes 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and o-nitrophenyl-beta-D-galactopyranoside (ONPG). Has activity against p-nitrophenyl(pNP)-beta-D-galactoside, but not significantly at all towards pNP-alpha-D-galactoside, pNP-beta-D-glucoside, pNP-beta-D-mannoside, pNP-beta-L-fucoside, pNP-beta-D-xyloside, pNP-beta-L-arabinoside, pNP-beta-D-galuronide, pNP-beta-D-glucuronide, pNP-beta-D-lactoside or pNP-beta-D-cellobioside. Ref.1

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Ref.1

Enzyme regulation

Activity stimulated by beta-mercaptoethanol. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 42 family.

Biophysicochemical properties

Kinetic parameters:

KM=0.57 mM for ONPG Ref.1

KM=4.81 mM for lactose

Vmax=254 µmol/min/mg enzyme with ONPG as substrate

Vmax=3.97 µmol/min/mg enzyme with lactose as substrate

pH dependence:

Optimum pH is 6.6. Maintains activity over a broad range of pH values from 6 to 9.

Temperature dependence:

Optimum temperature is 45-50 degrees Celsius. Activity declines rapidly above 50 degrees Celsius. Stable for at least 70 hours at temperatures 35 degrees Celsius and below. At 50 degrees Celsius loses all activity in less than 15 minutes.

Sequence caution

The sequence AAA75601.1 differs from that shown. Reason: Frameshift at positions 111, 158 and 637.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processgalactose metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 690690Beta-galactosidase
PRO_0000407680

Sites

Active site1741Proton donor By similarity
Binding site1731Substrate By similarity
Binding site3451Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q44233 [UniParc].

Last modified May 3, 2011. Version 2.
Checksum: 83262445ECE19128

FASTA69076,115
        10         20         30         40         50         60 
MPVPTPLSEG TTPDTAAQEL RTNRLWEALP GLSYGGDYIP NSGRNRSARK IYRSCRKPEC 

        70         80         90        100        110        120 
RPSALASSPG LGLEPVEGSY DFTWLDEVMD NLAATGIKVA LATATAAPPA GWLRKHPEIL 

       130        140        150        160        170        180 
PVTAEGSTLG PARAHYLVVG MVLFCRPVCG EDDPRLGERY KDHPALALWH VDNELGCHVS 

       190        200        210        220        230        240 
EFYGPRRHRR FPSMAEPTLR HDRGPQRGLG TAFWSQRYSC FEEILTPRPA PTTLNPTQQL 

       250        260        270        280        290        300 
DFQRFSSWGL IDFYSMLARG HFARSHPRCP PRQIWWPQAP PCLWDYFDWA KKLECHRQWS 

       310        320        330        340        350        360 
LPGGRRYRCV TSELAFRRRS DSEAIAGGKP WSPDGALSPC RPCNWLASQH DSRTPGEMAR 

       370        380        390        400        410        420 
NSLVHVGRGI WMLSCFSSGD RASRVRRNST RPWCRTPEPT REYGVKLLSW AQAQSLVRGS 

       430        440        450        460        470        480 
RRRGGITHRN RLRLRTLVGK RTGLHPAPMW KYLELLRAFH APCSCPASPP IWSIPALTLT 

       490        500        510        520        530        540 
AMTWWSSRPC TPSPMPRPAI LRQRQNAEPQ CSSATSVDID ENDAVRLGGY PGAFRDLLGV 

       550        560        570        580        590        600 
NVEEFHPLPE NSTVSLDAGW SGRIWSEHVH LTGAEAKVSF TEAPLTGVPA VTRHAVGTGA 

       610        620        630        640        650        660 
AWYLATFPDA TGLESLLDSL IAESGVRAPA MAAAGVELSR RSHADGRSYL FAINHNVTEA 

       670        680        690 
AVSAQGTELI SGTPFNGTVP AGAVAVIAEG 

« Hide

References

[1]"Analysis of a novel gene and beta-galactosidase isozyme from a psychrotrophic Arthrobacter isolate."
Gutshall K.R., Trimbur D.E., Kasmir J.J., Brenchley J.E.
J. Bacteriol. 177:1981-1988(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 9-16, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
Strain: B7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U17417 Genomic DNA. Translation: AAA75601.1. Frameshift.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFPIRSF001084. B-galactosidase. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGAL1_ARTSP
AccessionPrimary (citable) accession number: Q44233
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 3, 2011
Last modified: October 16, 2013
This is version 49 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries