SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q44233

- BGAL1_ARTSP

UniProt

Q44233 - BGAL1_ARTSP

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Beta-galactosidase

Gene
N/A
Organism
Arthrobacter sp.
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Highly specific towards beta-D-galactoside substrates. Hydrolyzes 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and o-nitrophenyl-beta-D-galactopyranoside (ONPG). Has activity against p-nitrophenyl(pNP)-beta-D-galactoside, but not significantly at all towards pNP-alpha-D-galactoside, pNP-beta-D-glucoside, pNP-beta-D-mannoside, pNP-beta-L-fucoside, pNP-beta-D-xyloside, pNP-beta-L-arabinoside, pNP-beta-D-galuronide, pNP-beta-D-glucuronide, pNP-beta-D-lactoside or pNP-beta-D-cellobioside.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Enzyme regulationi

Activity stimulated by beta-mercaptoethanol.1 Publication

Kineticsi

  1. KM=0.57 mM for ONPG1 Publication
  2. KM=4.81 mM for lactose

Vmax=254 µmol/min/mg enzyme with ONPG as substrate

Vmax=3.97 µmol/min/mg enzyme with lactose as substrate

pH dependencei

Optimum pH is 6.6. Maintains activity over a broad range of pH values from 6 to 9.

Temperature dependencei

Optimum temperature is 45-50 degrees Celsius. Activity declines rapidly above 50 degrees Celsius. Stable for at least 70 hours at temperatures 35 degrees Celsius and below. At 50 degrees Celsius loses all activity in less than 15 minutes.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei173 – 1731Substrate By similarity
Active sitei174 – 1741Proton donor By similarity
Binding sitei345 – 3451Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
OrganismiArthrobacter sp.
Taxonomic identifieri1667 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690Beta-galactosidasePRO_0000407680Add
BLAST

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q44233-1 [UniParc]FASTAAdd to Basket

« Hide

MPVPTPLSEG TTPDTAAQEL RTNRLWEALP GLSYGGDYIP NSGRNRSARK    50
IYRSCRKPEC RPSALASSPG LGLEPVEGSY DFTWLDEVMD NLAATGIKVA 100
LATATAAPPA GWLRKHPEIL PVTAEGSTLG PARAHYLVVG MVLFCRPVCG 150
EDDPRLGERY KDHPALALWH VDNELGCHVS EFYGPRRHRR FPSMAEPTLR 200
HDRGPQRGLG TAFWSQRYSC FEEILTPRPA PTTLNPTQQL DFQRFSSWGL 250
IDFYSMLARG HFARSHPRCP PRQIWWPQAP PCLWDYFDWA KKLECHRQWS 300
LPGGRRYRCV TSELAFRRRS DSEAIAGGKP WSPDGALSPC RPCNWLASQH 350
DSRTPGEMAR NSLVHVGRGI WMLSCFSSGD RASRVRRNST RPWCRTPEPT 400
REYGVKLLSW AQAQSLVRGS RRRGGITHRN RLRLRTLVGK RTGLHPAPMW 450
KYLELLRAFH APCSCPASPP IWSIPALTLT AMTWWSSRPC TPSPMPRPAI 500
LRQRQNAEPQ CSSATSVDID ENDAVRLGGY PGAFRDLLGV NVEEFHPLPE 550
NSTVSLDAGW SGRIWSEHVH LTGAEAKVSF TEAPLTGVPA VTRHAVGTGA 600
AWYLATFPDA TGLESLLDSL IAESGVRAPA MAAAGVELSR RSHADGRSYL 650
FAINHNVTEA AVSAQGTELI SGTPFNGTVP AGAVAVIAEG 690
Length:690
Mass (Da):76,115
Last modified:May 3, 2011 - v2
Checksum:i83262445ECE19128
GO

Sequence cautioni

The sequence AAA75601.1 differs from that shown. Reason: Frameshift at positions 111, 158 and 637.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17417 Genomic DNA. Translation: AAA75601.1. Frameshift.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17417 Genomic DNA. Translation: AAA75601.1 . Frameshift.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Analysis of a novel gene and beta-galactosidase isozyme from a psychrotrophic Arthrobacter isolate."
    Gutshall K.R., Trimbur D.E., Kasmir J.J., Brenchley J.E.
    J. Bacteriol. 177:1981-1988(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 9-16, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
    Strain: B7.

Entry informationi

Entry nameiBGAL1_ARTSP
AccessioniPrimary (citable) accession number: Q44233
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 3, 2011
Last modified: June 11, 2014
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3