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Q44233

- BGAL1_ARTSP

UniProt

Q44233 - BGAL1_ARTSP

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Protein

Beta-galactosidase

Gene
N/A
Organism
Arthrobacter sp.
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Highly specific towards beta-D-galactoside substrates. Hydrolyzes 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and o-nitrophenyl-beta-D-galactopyranoside (ONPG). Has activity against p-nitrophenyl(pNP)-beta-D-galactoside, but not significantly at all towards pNP-alpha-D-galactoside, pNP-beta-D-glucoside, pNP-beta-D-mannoside, pNP-beta-L-fucoside, pNP-beta-D-xyloside, pNP-beta-L-arabinoside, pNP-beta-D-galuronide, pNP-beta-D-glucuronide, pNP-beta-D-lactoside or pNP-beta-D-cellobioside.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Enzyme regulationi

Activity stimulated by beta-mercaptoethanol.1 Publication

Kineticsi

  1. KM=0.57 mM for ONPG1 Publication
  2. KM=4.81 mM for lactose1 Publication

Vmax=254 µmol/min/mg enzyme with ONPG as substrate1 Publication

Vmax=3.97 µmol/min/mg enzyme with lactose as substrate1 Publication

pH dependencei

Optimum pH is 6.6. Maintains activity over a broad range of pH values from 6 to 9.1 Publication

Temperature dependencei

Optimum temperature is 45-50 degrees Celsius. Activity declines rapidly above 50 degrees Celsius. Stable for at least 70 hours at temperatures 35 degrees Celsius and below. At 50 degrees Celsius loses all activity in less than 15 minutes.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei173 – 1731SubstrateBy similarity
Active sitei174 – 1741Proton donorBy similarity
Binding sitei345 – 3451SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-galBy similarity
OrganismiArthrobacter sp.
Taxonomic identifieri1667 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690Beta-galactosidasePRO_0000407680Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q44233 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPVPTPLSEG TTPDTAAQEL RTNRLWEALP GLSYGGDYIP NSGRNRSARK
60 70 80 90 100
IYRSCRKPEC RPSALASSPG LGLEPVEGSY DFTWLDEVMD NLAATGIKVA
110 120 130 140 150
LATATAAPPA GWLRKHPEIL PVTAEGSTLG PARAHYLVVG MVLFCRPVCG
160 170 180 190 200
EDDPRLGERY KDHPALALWH VDNELGCHVS EFYGPRRHRR FPSMAEPTLR
210 220 230 240 250
HDRGPQRGLG TAFWSQRYSC FEEILTPRPA PTTLNPTQQL DFQRFSSWGL
260 270 280 290 300
IDFYSMLARG HFARSHPRCP PRQIWWPQAP PCLWDYFDWA KKLECHRQWS
310 320 330 340 350
LPGGRRYRCV TSELAFRRRS DSEAIAGGKP WSPDGALSPC RPCNWLASQH
360 370 380 390 400
DSRTPGEMAR NSLVHVGRGI WMLSCFSSGD RASRVRRNST RPWCRTPEPT
410 420 430 440 450
REYGVKLLSW AQAQSLVRGS RRRGGITHRN RLRLRTLVGK RTGLHPAPMW
460 470 480 490 500
KYLELLRAFH APCSCPASPP IWSIPALTLT AMTWWSSRPC TPSPMPRPAI
510 520 530 540 550
LRQRQNAEPQ CSSATSVDID ENDAVRLGGY PGAFRDLLGV NVEEFHPLPE
560 570 580 590 600
NSTVSLDAGW SGRIWSEHVH LTGAEAKVSF TEAPLTGVPA VTRHAVGTGA
610 620 630 640 650
AWYLATFPDA TGLESLLDSL IAESGVRAPA MAAAGVELSR RSHADGRSYL
660 670 680 690
FAINHNVTEA AVSAQGTELI SGTPFNGTVP AGAVAVIAEG
Length:690
Mass (Da):76,115
Last modified:May 3, 2011 - v2
Checksum:i83262445ECE19128
GO

Sequence cautioni

The sequence AAA75601.1 differs from that shown. Reason: Frameshift at positions 111, 158 and 637.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17417 Genomic DNA. Translation: AAA75601.1. Frameshift.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17417 Genomic DNA. Translation: AAA75601.1 . Frameshift.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Analysis of a novel gene and beta-galactosidase isozyme from a psychrotrophic Arthrobacter isolate."
    Gutshall K.R., Trimbur D.E., Kasmir J.J., Brenchley J.E.
    J. Bacteriol. 177:1981-1988(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 9-16, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
    Strain: B71 Publication.

Entry informationi

Entry nameiBGAL1_ARTSP
AccessioniPrimary (citable) accession number: Q44233
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 3, 2011
Last modified: October 1, 2014
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3