Reviewed, UniProtKB/Swiss-Prot Q44088 (GYRB_ACILW)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit B
    EC=5.99.1.3

Gene names

Name:

gyrB

Organism

Acinetobacter lwoffii

Taxonomic identifier

28090 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	390 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the type II topoisomerase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›390	›390	DNA gyrase subunit B		PRO_0000145279
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	390	1

Sequences

	Sequence		Length	Mass (Da)
	Q44088-1 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 0BEC6AB97F6F989B	FASTA	390	43,147
	10 20 30 40 50 60 DNSYKVSGGL HGVGVSVVNA LSEKLELTIH RAGKIHEQEY RHGDSQYPLK VVGDTNRTGT 70 80 90 100 110 120 RVRFWPSAET FSQTIFNVDI LARRLRELSF LNAGVRIVLR DERINAEHVF DYEGGLSEFV 130 140 150 160 170 180 KYINEGKTHL NDIFHFTAAQ ADNGITVEVA LQWNDSYQEN VRCFTNNIPQ KDGGTHLAGF 190 200 210 220 230 240 RAALTRGLNN YMDSENILKK EKVAVSGDDA REGLTAIVSV KVPDPKFSSQ TKEKLVSSEV 250 260 270 280 290 300 KTAVEQAMNK AFSEYLLENP QAAKAIAGKI IDAARARDAA RKAREMTRRK SALDIAGLPG 310 320 330 340 350 360 KLADCQEKDP ALSELYLVEG DSAGGSAKQG RNRKMQAILP LKGKILNVER ARFDRMISSA 370 380 390 EVGTLITALG CGIGREEYNP DKLRYHKIII

References

[1]	"Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization." Yamamoto S., Bouvet P.J.M., Harayama S. Int. J. Syst. Bacteriol. 49:87-95(1999) [PubMed: 10028249] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15309 / NCTC 5866 / CIP 64.10.
[2]	"Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products." Yamamoto S., Harayama S. Int. J. Syst. Bacteriol. 46:506-511(1996) [PubMed: 8934907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-118 AND 290-389. Strain: ATCC 15309 / NCTC 5866 / CIP 64.10.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB008694 Genomic DNA. Translation: BAA75411.1. D73434 Genomic DNA. Translation: BAA11159.1. D73419 Genomic DNA. Translation: BAA11144.1.
3D structure databases
HSSP	HSSP built from PDB template 1EI1 based on UniProtKB P06982.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.99.1.3. 96146.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA_B/N. IPR013759. Topo_IIA_B/N_ab. IPR013506. Topo_IIA_B_2. IPR018522. TopoIIA_CS. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
ProDom	PD149633. DNA_gyrase_B. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00433. TOP2c. 1 hit. [Graphical view]
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRB_ACILW

Accession

Primary (citable) accession number: Q44088
Secondary accession number(s): Q59125, Q9ZA00

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 11, 2001
Last modified:	September 22, 2009
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)