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Q44088 (GYRB_ACILW) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA gyrase subunit B

EC=5.99.1.3
Gene names
Name:gyrB
OrganismAcinetobacter lwoffii
Taxonomic identifier28090 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter

Protein attributes

Sequence length390 AA.
Sequence statusFragment.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01898

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01898

Subunit structure

Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.

Subcellular location

Cytoplasm Potential HAMAP MF_01898.

Sequence similarities

Belongs to the type II topoisomerase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionIsomerase
Topoisomerase
Gene Ontology (GO)
   Biological processDNA topological change

Inferred from electronic annotation. Source: InterPro

   Cellular componentchromosome

Inferred from electronic annotation. Source: InterPro

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase (ATP-hydrolyzing) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›390›390DNA gyrase subunit B HAMAP MF_01898
PRO_0000145279

Experimental info

Non-terminal residue11
Non-terminal residue3901

Sequences

Sequence LengthMass (Da)Tools
Q44088 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: 0BEC6AB97F6F989B

FASTA39043,147
        10         20         30         40         50         60 
DNSYKVSGGL HGVGVSVVNA LSEKLELTIH RAGKIHEQEY RHGDSQYPLK VVGDTNRTGT 

        70         80         90        100        110        120 
RVRFWPSAET FSQTIFNVDI LARRLRELSF LNAGVRIVLR DERINAEHVF DYEGGLSEFV 

       130        140        150        160        170        180 
KYINEGKTHL NDIFHFTAAQ ADNGITVEVA LQWNDSYQEN VRCFTNNIPQ KDGGTHLAGF 

       190        200        210        220        230        240 
RAALTRGLNN YMDSENILKK EKVAVSGDDA REGLTAIVSV KVPDPKFSSQ TKEKLVSSEV 

       250        260        270        280        290        300 
KTAVEQAMNK AFSEYLLENP QAAKAIAGKI IDAARARDAA RKAREMTRRK SALDIAGLPG 

       310        320        330        340        350        360 
KLADCQEKDP ALSELYLVEG DSAGGSAKQG RNRKMQAILP LKGKILNVER ARFDRMISSA 

       370        380        390 
EVGTLITALG CGIGREEYNP DKLRYHKIII 

« Hide

References

[1]"Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization."
Yamamoto S., Bouvet P.J.M., Harayama S.
Int. J. Syst. Bacteriol. 49:87-95(1999) [PubMed: 10028249] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 15309 / NCTC 5866 / CIP 64.10.
[2]"Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products."
Yamamoto S., Harayama S.
Int. J. Syst. Bacteriol. 46:506-511(1996) [PubMed: 8934907] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-118 AND 290-389.
Strain: ATCC 15309 / NCTC 5866 / CIP 64.10.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB008694 Genomic DNA. Translation: BAA75411.1.
D73434 Genomic DNA. Translation: BAA11159.1.
D73419 Genomic DNA. Translation: BAA11144.1.

3D structure databases

ProteinModelPortalQ44088.
SMRQ44088. Positions 1-287.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

HAMAPMF_01898. GyrB.
[Tree]
InterProIPR003594. ATPase-like_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013759. Topo_IIA_B/N_ab.
IPR013506. Topo_IIA_bsu_dom2.
IPR013760. Topo_IIA_cen.
IPR018522. TopoIIA_CS.
[Graphical view]
Gene3DG3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
PfamPF00204. DNA_gyraseB. 1 hit.
[Graphical view]
PRINTSPR00418. TPI2FAMILY.
SMARTSM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMSSF55874. ATP_bd_ATPase. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
SSF56719. Topo_IIA_cen. 1 hit.
PROSITEPS00177. TOPOISOMERASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGYRB_ACILW
AccessionPrimary (citable) accession number: Q44088
Secondary accession number(s): Q59125, Q9ZA00
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 11, 2001
Last modified: January 25, 2012
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families