Reviewed, UniProtKB/Swiss-Prot Q44080 (GYRB_ACIJU)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit B
    EC=5.99.1.3

Gene names

Name:

gyrB

Organism

Acinetobacter junii

Taxonomic identifier

40215 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	388 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the type II topoisomerase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›388	›388	DNA gyrase subunit B		PRO_0000145278
Experimental info
	Sequence conflict	12	1	G → R in BAA11156. Ref.2
	Sequence conflict	303	1	D → G in BAA11141. Ref.2
	Non-terminal residue	1	1
	Non-terminal residue	388	1

Sequences

	Sequence		Length	Mass (Da)
	Q44080-1 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 7CDCB5E1659EE2B5	FASTA	388	42,970
	10 20 30 40 50 60 DNSYKVSGGL HGVGVSVVNA LSKKLHLTIQ RAGQIHEQEY CHGDPQYPLK VVGETDKTGT 70 80 90 100 110 120 TVRFWPSELT FSQTIFSVDI LARRLRELSF LNAGVRIVLR DERVNLENVY DYEGGLSEFV 130 140 150 160 170 180 KYINEGKTHL NEIFHFTTDA DNGIGVEVAL QWNDSYQENV RCFTNNIPQK DGGTHLAGFR 190 200 210 220 230 240 AALTRGLNSY MENENLLKKE KVAVSGDDAR EGLTAIISVK VPDPKFSSQT KEKLVSSEVK 250 260 270 280 290 300 PAVEQAMNKE FSAYLLENPQ AAKSIAGKII DAARARDAAR RAREMTRRKS ALDIAGLPGK 310 320 330 340 350 360 LADCQEKDPA LSELYLVEGD SAGGSAKQGR NRKMQAILPL KGKILNVERA RFDKMISSQE 370 380 VGTLITALGC GIGREEYNPD KLRYHKII

References

[1]	"Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization." Yamamoto S., Bouvet P.J.M., Harayama S. Int. J. Syst. Bacteriol. 49:87-95(1999) [PubMed: 10028249] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 17908 / NCTC 10307 / CIP 64.5 / B10 and SEIP 14.81.
[2]	"Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products." Yamamoto S., Harayama S. Int. J. Syst. Bacteriol. 46:506-511(1996) [PubMed: 8934907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-118 AND 289-388. Strain: ATCC 17908 / NCTC 10307 / CIP 64.5 / B10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB008693 Genomic DNA. Translation: BAA75410.1. AB008727 Genomic DNA. Translation: BAA75444.1. D73431 Genomic DNA. Translation: BAA11156.1. D73416 Genomic DNA. Translation: BAA11141.1.
PIR	T43906.
3D structure databases
SMR	Q44080. Positions 1-286, 294-388.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.99.1.3. 191731.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA_B/N. IPR013759. Topo_IIA_B/N_ab. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
Pfam	PF00204. DNA_gyraseB. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00433. TOP2c. 1 hit. [Graphical view]
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRB_ACIJU

Accession

Primary (citable) accession number: Q44080
Secondary accession number(s): Q59122, Q9ZA01

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 11, 2001
Last modified:	February 9, 2010
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)