Q44065 (GYRB_ACIHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit B
EC=5.99.1.3

Gene names

Name:

gyrB

Organism

Acinetobacter haemolyticus

Taxonomic identifier

29430 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	388 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01898
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01898
Subunit structure	Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01898.
Sequence similarities	Belongs to the type II topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›388	›388	DNA gyrase subunit B HAMAP-Rule MF_01898		PRO_0000145276
Regions
	Domain	312 – ›388	›77	Toprim
Sites
	Site	343	1	Interaction with DNA By similarity
	Site	346	1	Interaction with DNA By similarity
Experimental info
	Sequence conflict	12	1	G → R in BAA11155. Ref.2
	Non-terminal residue	1	1
	Non-terminal residue	388	1

Sequences

	Sequence		Length	Mass (Da)
	Q44065 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 076699E07CE72FBA	FASTA	388	43,103
	10 20 30 40 50 60 DNSYKVSGGL HGVGVSVVNA LSSKLQLTIH RAGQEHQQEY HHGDPQYPLR VIGETDRTGT 70 80 90 100 110 120 IVRFWPSAET FSQTIFNVDI LARRLRELSF LNAGVRIVLR DERVNLENVY DYEGGLSEFV 130 140 150 160 170 180 RYINEGKTHL NEIFHFTTDA DNGISVEVAL QWNDSYQENV RCFTNNIPQK DGGTHLAGFR 190 200 210 220 230 240 AALTRGLNSY MENENLLKKE KVVVSGDDAR EGLTAIISVK VPDPKFSSQT KEKLVSSEVK 250 260 270 280 290 300 PAVEQAMNKE FSAYLLENPQ AAKSIAGKII DAARARDAAR KAREMTRRKS ALDIAGLPGK 310 320 330 340 350 360 LADCQEKDPA LSELYLVEGD SAGGSAKQGR NRKMQAILPL KGKILNVERA RFDKMISSQE 370 380 VGTLITALGC GIGREEYNPD KLRYHKII

References

[1]	"Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization." Yamamoto S., Bouvet P.J.M., Harayama S. Int. J. Syst. Bacteriol. 49:87-95(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 17906 / NCTC 10305 / CIP 64.3 / B40 and ATCC 17907 / CIP 64.4 / B44.
[2]	"Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products." Yamamoto S., Harayama S. Int. J. Syst. Bacteriol. 46:506-511(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-118 AND 289-388. Strain: ATCC 17906 / NCTC 10305 / CIP 64.3 / B40.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB008691 Genomic DNA. Translation: BAA75408.1. AB008724 Genomic DNA. Translation: BAA75441.1. D73430 Genomic DNA. Translation: BAA11155.1. D73415 Genomic DNA. Translation: BAA11140.1.
PIR	T43904.
3D structure databases
ProteinModelPortal	Q44065.
SMR	Q44065. Positions 1-286.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.230.10. 1 hit. 3.30.565.10. 1 hit. 3.40.50.670. 1 hit.
HAMAP	MF_01898. GyrB.
InterPro	IPR003594. HATPase_ATP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR013506. Topo_IIA_bsu_dom2. IPR013759. Topo_IIA_cen_dom. IPR013760. Topo_IIA_like_dom. IPR018522. TopoIIA_CS. IPR006171. Toprim_domain. [Graphical view]
Pfam	PF00204. DNA_gyraseB. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00433. TOP2c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF56719. Topo_IIA_cen. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. PS50880. TOPRIM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRB_ACIHA

Accession

Primary (citable) accession number: Q44065
Secondary accession number(s): Q59120, Q9ZA03

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 11, 2001
Last modified:	May 1, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program