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Protein

Aminoglycoside N(6')-acetyltransferase type 1

Gene
N/A
Organism
Acinetobacter haemolyticus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of an acetyl group from acetyl-CoA to the 6'-amino group of aminoglycoside molecules conferring resistance to antibiotics containing the purpurosamine ring including amikacin, kanamycin, tobramycin and netilmicin.1 Publication

Catalytic activityi

Acetyl-CoA + kanamycin-B = CoA + N(6')-acetylkanamycin-B.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221SubstrateBy similarity
Binding sitei65 – 651SubstrateBy similarity
Binding sitei78 – 781SubstrateBy similarity
Binding sitei114 – 1141SubstrateBy similarity
Binding sitei119 – 1191Acetyl-CoABy similarity
Binding sitei135 – 1351SubstrateBy similarity

GO - Molecular functioni

  • aminoglycoside 6'-N-acetyltransferase activity Source: UniProtKB

GO - Biological processi

  • response to antibiotic Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoglycoside N(6')-acetyltransferase type 11 Publication (EC:2.3.1.821 Publication)
Alternative name(s):
AAC(6')-Ig1 Publication
Aminoglycoside resistance protein1 Publication
OrganismiAcinetobacter haemolyticus
Taxonomic identifieri29430 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 145145Aminoglycoside N(6')-acetyltransferase type 1PRO_0000416830Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

Secondary structure

145
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 55Combined sources
Helixi8 – 103Combined sources
Helixi11 – 2111Combined sources
Helixi26 – 3712Combined sources
Beta strandi42 – 498Combined sources
Beta strandi52 – 6211Combined sources
Beta strandi70 – 8213Combined sources
Helixi84 – 863Combined sources
Helixi91 – 10515Combined sources
Beta strandi110 – 1167Combined sources
Helixi120 – 1289Combined sources
Beta strandi132 – 14312Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EVYX-ray1.77A/B1-145[»]
4F0YX-ray2.56A/B1-145[»]
ProteinModelPortaliQ44057.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 145145N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 823Acetyl-CoA bindingBy similarity

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK18816.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR024170. Aminoglycoside_N6-AcTrfrase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000452. 6-N-acetyltransf. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q44057-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIKPASEAS LKDWLELRNK LWSDSEASHL QEMHQLLAEK YALQLLAYSD
60 70 80 90 100
HQAIAMLEAS IRFEYVNGTE TSPVGFLEGI YVLPAHRRSG VATMLIRQAE
110 120 130 140
VWAKQFSCTE FASDAALDNV ISHAMHRSLG FQETEKVVYF SKKID
Length:145
Mass (Da):16,448
Last modified:November 1, 1996 - v1
Checksum:iBAE955A9EABFFA40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09246 Genomic DNA. Translation: AAA21889.1.
PIRiI39502.
RefSeqiWP_005081764.1. NZ_BBSE01000020.1.

Genome annotation databases

GeneIDi23298302.
KEGGiag:AAA21889.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09246 Genomic DNA. Translation: AAA21889.1.
PIRiI39502.
RefSeqiWP_005081764.1. NZ_BBSE01000020.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EVYX-ray1.77A/B1-145[»]
4F0YX-ray2.56A/B1-145[»]
ProteinModelPortaliQ44057.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23298302.
KEGGiag:AAA21889.

Phylogenomic databases

KOiK18816.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR024170. Aminoglycoside_N6-AcTrfrase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000452. 6-N-acetyltransf. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAAC6_ACIHA
AccessioniPrimary (citable) accession number: Q44057
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: November 1, 1996
Last modified: November 11, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.