Reviewed, UniProtKB/Swiss-Prot Q44056 (BLA1_AERHY)

Names and origin

Protein names

Recommended name:
    Beta-lactamase AER-1
    EC=3.5.2.6
Alternative name(s):
    Penicillinase

Gene names

Name:

aer1

Organism

Aeromonas hydrophila

Taxonomic identifier

644 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes carbenicillin. Methicillin and oxacillin are weakly hydrolyzed.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Transposable element
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 37	37	Potential
	Chain	38 – 304	267	Beta-lactamase AER-1		PRO_0000017048
Regions
	Region	248 – 250	3	Substrate binding By similarity
Sites
	Active site	83	1	Acyl-ester intermediate By similarity
Amino acid modifications
	Disulfide bond	90 ↔ 137		Potential

Sequences

	Sequence		Length	Mass (Da)
	Q44056-1 [UniParc]. Last modified August 1, 1998. Version 2. Checksum: 66AD56ED45D02A41	FASTA	304	32,428
	10 20 30 40 50 60 MYVLSVEKPT LRNKFAAGIG VVLVCVVASF IPTPVFALDT TKLIQAVQSE ESALHARVGM 70 80 90 100 110 120 TVFDSNTGTT WNYRGDERFP LNSTHKTFSC AALLAKVDGK SLSLGQSVSI SKEMLVTYSP 130 140 150 160 170 180 ITEKSLSPET VTFGKICQAA VSYSDNTAAN VVFDAIGGAT GFNAYMRSIG DEETQLDRKE 190 200 210 220 230 240 PELNEGTPGD VRDTTTPNAM VNSLRKILLG DALSASSRSQ LTQWMLDDQV AGALLRASLP 250 260 270 280 290 300 SDWKIADKTG AGGYGSRSIV AVIWPPSKQP LVVGIYITQT KASMQASNQA IARIGVVLKD TVAP

References

[1]	"Structure of CARB-4 and AER-1 carbenicillin-hydrolyzing beta-lactamases." Sanschagrin F., Bejaoui N., Levesque R.C. Antimicrob. Agents Chemother. 42:1966-1972(1998) [PubMed: 9687391] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: VL7711. Transposon: Omega7711.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U14748 Genomic DNA. Translation: AAC09015.1.
3D structure databases
HSSP	HSSP built from PDB template 1JTG based on UniProtKB P00810.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 3060.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA1_AERHY

Accession

Primary (citable) accession number: Q44056

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	September 22, 2009
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)