Reviewed, UniProtKB/Swiss-Prot Q44052 (IMD_ARTGO)

Names and origin

Protein names

Recommended name:
    Isomalto-dextranase
    EC=3.2.1.94
Alternative name(s):
    Glucan 1,6-alpha-isomaltosidase
    Exo-isomaltohydrolase

Gene names

Name:

imd

Organism

Arthrobacter globiformis

Taxonomic identifier

1665 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->6)-alpha-D-glucosidic linkages in polysaccharides, to remove successive isomaltose units from the non-reducing ends of the chains.
Subcellular location	Secreted.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family. Contains 1 CBM6 (carbohydrate binding type-6) domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro glucan 1,6-alpha-isomaltosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	Tat-type signal
	Chain	40 – 641	602	Isomalto-dextranase		PRO_0000001022
Regions
	Domain	500 – 640	141	CBM6
Sites
	Active site	227	1	Nucleophile By similarity
	Active site	288	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q44052-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B7F8F278F4D88350	FASTA	641	69,765
	10 20 30 40 50 60 MMNLSRRTLL TTGSAATLAY ALGMAGSAQA ATAVTARPGV PVTAAPPLRL ASRNSVFTRS 70 80 90 100 110 120 GAGPRYWNIY GYSFPHNAPI PENEWKANID WLAGNFADFG YDIACTDGWI EGSSRTTGNG 130 140 150 160 170 180 YITSYNDSWQ HDWAYWANYL AARKMKLGVY YNPLWVHRAA VEDASKTVLG RPDVKIADLV 190 200 210 220 230 240 VPGDFFARDI GGNQLYWLDV TKSGAKEYVQ GYVRYFKDLG VPYLRIDFLS WYEDGRDANI 250 260 270 280 290 300 GQVNAPHGRA NYELALSWIN EAAGEDMEVS LVMPHMFQDG SAELANGDLV RINADADKGG 310 320 330 340 350 360 WDRLSGMRQN WQDAWPNWAN PFCGFTGWSH RNGRGQLILD GDFMRASTFA SDEERKTMMN 370 380 390 400 410 420 LMVAAGSPLA IADTYQQIGN NAWVYTNKEV LQLNADGLVG KPLYRSATPF SKDPGSRDTE 430 440 450 460 470 480 RWAGQLPDGS WGVALFNRSD TETVTKTIDF AKDLGLATGG NVRDLWEHRN LGMDSRATAA 490 500 510 520 530 540 LAPHASAIFR VTPPKMHGTT RYPAAFAAWG GGAGFNNNHP GYDGNGFVDG LQAGSGSADP 550 560 570 580 590 600 LVTFAVQVPH RAATPSGYRY ANATDDNTTS KTTTKKANPE KADRSTVDGP VHVSFPGLAT 610 620 630 640 WDTWGVAAGT ITLDAGLNLV TIGRGATDKG AINLNWIELD M

References

[1]

"Molecular cloning and expression of an isomalto-dextranase gene from Arthrobacter globiformis T6." Iwai A., Ito H., Mizuno T., Mori H., Matsui H., Honma M., Okada G., Chiba S. J. Bacteriol. 176:7730-7734(1994) [PubMed: 8002600] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: T6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D30761 Genomic DNA. Translation: BAA06424.1.
PIR	A55549.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	CBM35. Carbohydrate-Binding Module Family 35. GH27. Glycoside Hydrolase Family 27.
Enzyme and pathway databases
BRENDA	3.2.1.94. 1012.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR005084. CBM_6. IPR000111. Glyco_hydro_GHD. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00512. ALPHA_GALACTOSIDASE. 1 hit. PS51175. CBM6. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IMD_ARTGO

Accession

Primary (citable) accession number: Q44052

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)