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Q44052

- IMD_ARTGO

UniProt

Q44052 - IMD_ARTGO

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Protein

Isomalto-dextranase

Gene

imd

Organism
Arthrobacter globiformis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in polysaccharides, to remove successive isomaltose units from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei227 – 2271NucleophileBy similarity
Active sitei288 – 2881Proton donorBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glucan 1,6-alpha-isomaltosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiCBM35. Carbohydrate-Binding Module Family 35.
GH27. Glycoside Hydrolase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Isomalto-dextranase (EC:3.2.1.94)
Alternative name(s):
Exo-isomaltohydrolase
Glucan 1,6-alpha-isomaltosidase
Gene namesi
Name:imd
OrganismiArthrobacter globiformis
Taxonomic identifieri1665 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3939Tat-type signalAdd
BLAST
Chaini40 – 641602Isomalto-dextranasePRO_0000001022Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Structurei

3D structure databases

ProteinModelPortaliQ44052.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini500 – 640141CBM6PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated
Contains 1 CBM6 (carbohydrate binding type-6) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR005084. CMB_fam6.
IPR008979. Galactose-bd-like.
IPR013780. Glyco_hydro_13_b.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
IPR006311. TAT_signal.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 2 hits.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
PS51175. CBM6. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q44052-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMNLSRRTLL TTGSAATLAY ALGMAGSAQA ATAVTARPGV PVTAAPPLRL
60 70 80 90 100
ASRNSVFTRS GAGPRYWNIY GYSFPHNAPI PENEWKANID WLAGNFADFG
110 120 130 140 150
YDIACTDGWI EGSSRTTGNG YITSYNDSWQ HDWAYWANYL AARKMKLGVY
160 170 180 190 200
YNPLWVHRAA VEDASKTVLG RPDVKIADLV VPGDFFARDI GGNQLYWLDV
210 220 230 240 250
TKSGAKEYVQ GYVRYFKDLG VPYLRIDFLS WYEDGRDANI GQVNAPHGRA
260 270 280 290 300
NYELALSWIN EAAGEDMEVS LVMPHMFQDG SAELANGDLV RINADADKGG
310 320 330 340 350
WDRLSGMRQN WQDAWPNWAN PFCGFTGWSH RNGRGQLILD GDFMRASTFA
360 370 380 390 400
SDEERKTMMN LMVAAGSPLA IADTYQQIGN NAWVYTNKEV LQLNADGLVG
410 420 430 440 450
KPLYRSATPF SKDPGSRDTE RWAGQLPDGS WGVALFNRSD TETVTKTIDF
460 470 480 490 500
AKDLGLATGG NVRDLWEHRN LGMDSRATAA LAPHASAIFR VTPPKMHGTT
510 520 530 540 550
RYPAAFAAWG GGAGFNNNHP GYDGNGFVDG LQAGSGSADP LVTFAVQVPH
560 570 580 590 600
RAATPSGYRY ANATDDNTTS KTTTKKANPE KADRSTVDGP VHVSFPGLAT
610 620 630 640
WDTWGVAAGT ITLDAGLNLV TIGRGATDKG AINLNWIELD M
Length:641
Mass (Da):69,765
Last modified:November 1, 1996 - v1
Checksum:iB7F8F278F4D88350
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30761 Genomic DNA. Translation: BAA06424.1.
PIRiA55549.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30761 Genomic DNA. Translation: BAA06424.1 .
PIRi A55549.

3D structure databases

ProteinModelPortali Q44052.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM35. Carbohydrate-Binding Module Family 35.
GH27. Glycoside Hydrolase Family 27.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.70. 2 hits.
InterProi IPR013785. Aldolase_TIM.
IPR005084. CMB_fam6.
IPR008979. Galactose-bd-like.
IPR013780. Glyco_hydro_13_b.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
IPR006311. TAT_signal.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 2 hits.
PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
PS51175. CBM6. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of an isomalto-dextranase gene from Arthrobacter globiformis T6."
    Iwai A., Ito H., Mizuno T., Mori H., Matsui H., Honma M., Okada G., Chiba S.
    J. Bacteriol. 176:7730-7734(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: T6.

Entry informationi

Entry nameiIMD_ARTGO
AccessioniPrimary (citable) accession number: Q44052
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3