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Q44052

- IMD_ARTGO

UniProt

Q44052 - IMD_ARTGO

Protein

Isomalto-dextranase

Gene

imd

Organism
Arthrobacter globiformis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->6)-alpha-D-glucosidic linkages in polysaccharides, to remove successive isomaltose units from the non-reducing ends of the chains.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei227 – 2271NucleophileBy similarity
    Active sitei288 – 2881Proton donorBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. glucan 1,6-alpha-isomaltosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiCBM35. Carbohydrate-Binding Module Family 35.
    GH27. Glycoside Hydrolase Family 27.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isomalto-dextranase (EC:3.2.1.94)
    Alternative name(s):
    Exo-isomaltohydrolase
    Glucan 1,6-alpha-isomaltosidase
    Gene namesi
    Name:imd
    OrganismiArthrobacter globiformis
    Taxonomic identifieri1665 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3939Tat-type signalAdd
    BLAST
    Chaini40 – 641602Isomalto-dextranasePRO_0000001022Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

    Structurei

    3D structure databases

    ProteinModelPortaliQ44052.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini500 – 640141CBM6PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 27 family.Curated
    Contains 1 CBM6 (carbohydrate binding type-6) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.70. 2 hits.
    InterProiIPR013785. Aldolase_TIM.
    IPR005084. CMB_fam6.
    IPR008979. Galactose-bd-like.
    IPR013780. Glyco_hydro_13_b.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    IPR006311. TAT_signal.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 2 hits.
    PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
    PS51175. CBM6. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q44052-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMNLSRRTLL TTGSAATLAY ALGMAGSAQA ATAVTARPGV PVTAAPPLRL    50
    ASRNSVFTRS GAGPRYWNIY GYSFPHNAPI PENEWKANID WLAGNFADFG 100
    YDIACTDGWI EGSSRTTGNG YITSYNDSWQ HDWAYWANYL AARKMKLGVY 150
    YNPLWVHRAA VEDASKTVLG RPDVKIADLV VPGDFFARDI GGNQLYWLDV 200
    TKSGAKEYVQ GYVRYFKDLG VPYLRIDFLS WYEDGRDANI GQVNAPHGRA 250
    NYELALSWIN EAAGEDMEVS LVMPHMFQDG SAELANGDLV RINADADKGG 300
    WDRLSGMRQN WQDAWPNWAN PFCGFTGWSH RNGRGQLILD GDFMRASTFA 350
    SDEERKTMMN LMVAAGSPLA IADTYQQIGN NAWVYTNKEV LQLNADGLVG 400
    KPLYRSATPF SKDPGSRDTE RWAGQLPDGS WGVALFNRSD TETVTKTIDF 450
    AKDLGLATGG NVRDLWEHRN LGMDSRATAA LAPHASAIFR VTPPKMHGTT 500
    RYPAAFAAWG GGAGFNNNHP GYDGNGFVDG LQAGSGSADP LVTFAVQVPH 550
    RAATPSGYRY ANATDDNTTS KTTTKKANPE KADRSTVDGP VHVSFPGLAT 600
    WDTWGVAAGT ITLDAGLNLV TIGRGATDKG AINLNWIELD M 641
    Length:641
    Mass (Da):69,765
    Last modified:November 1, 1996 - v1
    Checksum:iB7F8F278F4D88350
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30761 Genomic DNA. Translation: BAA06424.1.
    PIRiA55549.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30761 Genomic DNA. Translation: BAA06424.1 .
    PIRi A55549.

    3D structure databases

    ProteinModelPortali Q44052.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM35. Carbohydrate-Binding Module Family 35.
    GH27. Glycoside Hydrolase Family 27.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.70. 2 hits.
    InterProi IPR013785. Aldolase_TIM.
    IPR005084. CMB_fam6.
    IPR008979. Galactose-bd-like.
    IPR013780. Glyco_hydro_13_b.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    IPR006311. TAT_signal.
    [Graphical view ]
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 2 hits.
    PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
    PS51175. CBM6. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of an isomalto-dextranase gene from Arthrobacter globiformis T6."
      Iwai A., Ito H., Mizuno T., Mori H., Matsui H., Honma M., Okada G., Chiba S.
      J. Bacteriol. 176:7730-7734(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: T6.

    Entry informationi

    Entry nameiIMD_ARTGO
    AccessioniPrimary (citable) accession number: Q44052
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3