Reviewed, UniProtKB/Swiss-Prot Q44007 (CZCS_RALME)

Names and origin

Protein names

Recommended name:
    Sensor protein czcS
    EC=2.7.13.3

Gene names

Name:	czcS
Ordered Locus Names:	Rmet_5977

Encoded on

Plasmid pMOL30

Organism

Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) [Complete proteome] [HAMAP]

Taxonomic identifier

266264 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Member of the two-component regulatory system czcS/czcR involved in the control of cobalt, zinc and cadmium homeostasis. Probably activates czcR by phosphorylation.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Single-pass membrane protein Potential.
Induction	By cadmium, lead, and zinc.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal Transmembrane
Ligand	ATP-binding Cadmium Cobalt Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro sensory perception Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cadmium ion binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 476	441	Sensor protein czcS		PRO_0000074746
Regions
	Topological domain	37 – 158	122	Periplasmic Potential
	Transmembrane	159 – 179	21	Potential
	Topological domain	180 – 476	297	Cytoplasmic Potential
	Domain	181 – 234	54	HAMP
	Domain	242 – 455	214	Histidine kinase
Amino acid modifications
	Modified residue	245	1	Phosphohistidine; by autocatalysis By similarity
Experimental info
	Sequence conflict	377 – 379	3	EAA → DR in CAA67087. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q44007-1 [UniParc]. Last modified July 11, 2006. Version 2. Checksum: 657329F4162C6326	FASTA	476	52,010
	10 20 30 40 50 60 MRPGTSITPL SLTRRLGLFF ALVLSIALAS MGAFAYYSLA AQLEARDDEV VKGKLEQVEH 70 80 90 100 110 120 FLREVDGVQG VPAAQHRFDD LVRGYSDLIV RVTALDGRLL FRTGNDALLE GTDQAAVTGK 130 140 150 160 170 180 SSLMFQSADA VLGRDGTRAT VFVAKSGEDR KQVTARFRTT LVLGTTVGVI LTALVGAAIT 190 200 210 220 230 240 RRELEPAHVL IKQINRISVE RLSYRVDMPP KPTEVRDIAS AFNAMLQRLE DGYQKLSRFS 250 260 270 280 290 300 ADLAHDLRTP LNNLIGHAEV ALSRDRTGPE YVALVEESLV EYQRLARMID AMLFLARADS 310 320 330 340 350 360 ANVALELTEL QLNAELRKLS AYFSVLAEER SVVIRVSGDA TLVADAILFQ RAINNVLSNA 370 380 390 400 410 420 VRHAWPNSMI DLVVRREAAH CCIDITNVGD PIPERELSLI FDRFFRGDRA RSNSSQSTGL 430 440 450 460 470 GLAIVLSIME LHGGDASAVS GLDGKTRFTL RFPLNGAEAS ARVSVGRPSQ DRPVVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two-component regulatory system involved in transcriptional control of heavy-metal homoeostasis in Alcaligenes eutrophus." van der Lelie D., Schwuchow T., Schwidetzky T., Wuertz S., Baeyens W., Mergeay M., Nies D.H. Mol. Microbiol. 23:493-503(1997) [PubMed: 9044283] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and features of the Ralstonia metallidurans CH34 heavy metals plasmids pMOL28 and pMOL30." Monchy S., van der Lelie D., Vallaeys T., Taghavi S., Benotmane M., McCorkle S., Dunn J., Lapidus A., Mergeay M. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Complete sequence of plasmid pMOL30 of Ralstonia metallidurans CH34." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Martinez M., Goltsman E., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Mergeay M., Benotmane M.A., Vallaeys T., Michaux A., Monchy S., Dunn J., McCorkle S., Taghavi S., van der Lelie D., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X71400 Genomic DNA. Translation: CAI11247.1. X98451 Genomic DNA. Translation: CAA67087.1. CP000354 Genomic DNA. Translation: ABF12836.1.
RefSeq	YP_145598.1. YP_581786.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3170507. 4036512.
GenomeReviews	Gene locus Rmet_5977 in contig CP000354_GR.
KEGG	rme:Rmet_5977.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q44007.
OMA	Q44007. DSNIEEC.
Enzyme and pathway databases
BioCyc	RMET266264:RMET_5977-MON.
BRENDA	2.7.13.3. 1263.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR006290. Sig_transdc_His_kin_metal-sen. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01386. cztS_silS_copS. 1 hit.
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CZCS_RALME

Accession

Primary (citable) accession number: Q44007
Secondary accession number(s): Q58AM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 11, 2006
Last modified:	June 16, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)