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Q44002 (DHET_ACEEU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 27, 2011. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alcohol dehydrogenase [cytochrome c]

EC=1.1.2.8
Gene names
Name:adh
OrganismAcetobacter europaeus (Gluconacetobacter europaeus)
Taxonomic identifier33995 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconacetobacter

Protein attributes

Sequence length739 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of primary alcohols except for methanol that is not a substrate By similarity.

Catalytic activity

A primary alcohol + 2 cytochrome c = an aldehyde + 2 reduced cytochrome c.

Cofactor

Binds 1 PQQ group per subunit. PQQ is inserted between disulfide Cys-143-Cys-144 and the indole ring of Trp-281 By similarity.

Binds 1 calcium ion per subunit By similarity.

Binds 1 heme group per subunit By similarity.

Subunit structure

Heterotetramer (dehydrogenase, cytochrome, and two smaller unknown subunits) that forms the alcohol dehydrogenase complex By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein; Periplasmic side Potential.

Sequence similarities

Belongs to the bacterial PQQ dehydrogenase family.

Contains 1 cytochrome c domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3535 Potential
Chain36 – 739704Alcohol dehydrogenase [cytochrome c]
PRO_0000025560

Regions

Domain635 – 739105Cytochrome c

Sites

Active site3441Proton acceptor Potential
Metal binding2171Calcium By similarity
Metal binding2991Calcium By similarity
Metal binding6551Iron (heme axial ligand) By similarity
Binding site6511Heme (covalent) By similarity
Binding site6541Heme (covalent) By similarity

Amino acid modifications

Disulfide bond143 ↔ 144 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q44002 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E681BB237ACB91F4

FASTA73980,945
        10         20         30         40         50         60 
MISAVFGKRR SLSRTLTAGT ICAALISGYA TMASADDGQG ATGEAIIHAD DHPGNWMTYG 

        70         80         90        100        110        120 
RTYSEQRYSP LDQINRSNVG NLKLAWYLDL DTNRGQEGTP LVIDGVMYAT TNWSMMKAVD 

       130        140        150        160        170        180 
AATGKLLWSY DPRVPGNIAD KGCCDTVNRG AAYWNGKVYF GTFDGRLIAL DAKTGKLVWS 

       190        200        210        220        230        240 
VNTIPPEAEL GKQRSYTVDG APRIAKGRVI IGNGGSEFGA RGFVTAFDAE TGKVDWRFFT 

       250        260        270        280        290        300 
APNPKNEPDH TASDSVLMNK AYQTWSPTGA WTRQGGGGTV WDSIVYDPVA DLVYLGVGNG 

       310        320        330        340        350        360 
SPWNYKYRSE GKGDNLFLGS IVALKPETGE YVWHFQETPM DQWDFTSVQQ IMTLDLPING 

       370        380        390        400        410        420 
ETRHVIVHAP KNGFFYIIDA KTGEFISGKN YVYVNWASGL DPKTGRPIYN PDALYTLTGK 

       430        440        450        460        470        480 
EWYGIPGDLG GHNFAAMAFS PKTGLVYIPA QQVPFLYTNQ VGGFTPHPDS WNLGLDMNKV 

       490        500        510        520        530        540 
GIPDSPEAKQ AFVKDLKGWI VAWDPQKQAE AWRVDHKGPW NGGILATGGD LLFQGLANGE 

       550        560        570        580        590        600 
FHAYDATNGS DLFHFAADSG IIAPPVTYLA NGKQYVAVEV GWGGIYPFFL GGLARTSGWT 

       610        620        630        640        650        660 
VNHSRIIAFS LDGKSGPLPK QNDQGFLPVK PPAQFDSKRT DNGYFQFQTY CAACHGDNAE 

       670        680        690        700        710        720 
GAGVLPDLRW SGSIRHEDAF YNVVGRGALT AYGMDRFDGN MNPTEIEDIR QFLIKRANET 

       730 
YQREVDARKN ADGIPEQLP 

« Hide

References

[1]Thurner C.A.K.
Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 51845 / DSM 6160 / DES11 / LMG 18890.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X82894 Genomic DNA. Translation: CAA58066.1.
Y09480 Genomic DNA. Translation: CAA70688.1.

3D structure databases

ProteinModelPortalQ44002.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR009056. Cyt_c_dom.
IPR003088. Cyt_c_I.
IPR019556. PQQ-dependent_C.
IPR019551. PQQ-dependent_N.
IPR018391. PQQ_beta_propeller_repeat.
IPR017512. PQQ_MeOH/EtOH_DH.
IPR002372. PQQ_repeat.
IPR011047. Quinonprotein_ADH-like.
IPR001479. Quinoprotein_DH_CS.
[Graphical view]
Gene3DG3DSA:1.10.760.10. Cytochrome_c_R. 1 hit.
G3DSA:2.140.10.10. Quinoprotein_alc_DH-like. 1 hit.
PfamPF00034. Cytochrom_C. 1 hit.
PF01011. PQQ. 4 hits.
PF10535. PQQ_C. 1 hit.
PF10527. PQQ_N. 1 hit.
[Graphical view]
SMARTSM00564. PQQ. 5 hits.
[Graphical view]
SUPFAMSSF46626. Cytochrome_c. 1 hit.
SSF50998. Quin_alc_DH_like. 1 hit.
TIGRFAMsTIGR03075. PQQ_enz_alc_DH. 1 hit.
PROSITEPS00363. BACTERIAL_PQQ_1. 1 hit.
PS00364. BACTERIAL_PQQ_2. 1 hit.
PS51007. CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHET_ACEEU
AccessionPrimary (citable) accession number: Q44002
Secondary accession number(s): O07952
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: July 27, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families