Reviewed, UniProtKB/Swiss-Prot Q44002 (DHET_ACEEU)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase [acceptor]
    EC=1.1.99.8

Gene names

Name:

adh

Organism

Acetobacter europaeus (Gluconacetobacter europaeus)

Taxonomic identifier

33995 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconacetobacter

Protein attributes

Sequence length	739 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A primary alcohol + acceptor = an aldehyde + reduced acceptor.
Cofactor	Binds 1 PQQ group per subunit. PQQ is inserted between disulfide Cys-143-Cys-144 and the indole ring of Trp-281 By similarity. Binds 1 calcium ion per subunit By similarity. Binds 1 heme group per subunit By similarity.
Subunit structure	Heterotetramer (dehydrogenase, cytochrome, and two smaller unknown subunits) that forms the alcohol dehydrogenase complex By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein; Periplasmic side Potential.
Sequence similarities	Belongs to the bacterial PQQ dehydrogenase family. Contains 1 cytochrome c domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond PQQ
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alcohol dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 739	704	Alcohol dehydrogenase [acceptor]		PRO_0000025560
Regions
	Domain	635 – 739	105	Cytochrome c
Sites
	Active site	344	1	Proton acceptor Potential
	Metal binding	217	1	Calcium By similarity
	Metal binding	299	1	Calcium By similarity
	Metal binding	655	1	Iron (heme axial ligand) By similarity
	Binding site	651	1	Heme (covalent) By similarity
	Binding site	654	1	Heme (covalent) By similarity
Amino acid modifications
	Disulfide bond	143 ↔ 144		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q44002-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E681BB237ACB91F4	FASTA	739	80,945
	10 20 30 40 50 60 MISAVFGKRR SLSRTLTAGT ICAALISGYA TMASADDGQG ATGEAIIHAD DHPGNWMTYG 70 80 90 100 110 120 RTYSEQRYSP LDQINRSNVG NLKLAWYLDL DTNRGQEGTP LVIDGVMYAT TNWSMMKAVD 130 140 150 160 170 180 AATGKLLWSY DPRVPGNIAD KGCCDTVNRG AAYWNGKVYF GTFDGRLIAL DAKTGKLVWS 190 200 210 220 230 240 VNTIPPEAEL GKQRSYTVDG APRIAKGRVI IGNGGSEFGA RGFVTAFDAE TGKVDWRFFT 250 260 270 280 290 300 APNPKNEPDH TASDSVLMNK AYQTWSPTGA WTRQGGGGTV WDSIVYDPVA DLVYLGVGNG 310 320 330 340 350 360 SPWNYKYRSE GKGDNLFLGS IVALKPETGE YVWHFQETPM DQWDFTSVQQ IMTLDLPING 370 380 390 400 410 420 ETRHVIVHAP KNGFFYIIDA KTGEFISGKN YVYVNWASGL DPKTGRPIYN PDALYTLTGK 430 440 450 460 470 480 EWYGIPGDLG GHNFAAMAFS PKTGLVYIPA QQVPFLYTNQ VGGFTPHPDS WNLGLDMNKV 490 500 510 520 530 540 GIPDSPEAKQ AFVKDLKGWI VAWDPQKQAE AWRVDHKGPW NGGILATGGD LLFQGLANGE 550 560 570 580 590 600 FHAYDATNGS DLFHFAADSG IIAPPVTYLA NGKQYVAVEV GWGGIYPFFL GGLARTSGWT 610 620 630 640 650 660 VNHSRIIAFS LDGKSGPLPK QNDQGFLPVK PPAQFDSKRT DNGYFQFQTY CAACHGDNAE 670 680 690 700 710 720 GAGVLPDLRW SGSIRHEDAF YNVVGRGALT AYGMDRFDGN MNPTEIEDIR QFLIKRANET 730 YQREVDARKN ADGIPEQLP

References

[1]	Thurner C.A.K. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 51845 / DSM 6160 / DES11 / LMG 18890.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X82894 Genomic DNA. Translation: CAA58066.1. Y09480 Genomic DNA. Translation: CAA70688.1.
3D structure databases
HSSP	HSSP built from PDB template 1KB0 based on UniProtKB Q46444.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.99.8. 264198.
Family and domain databases
InterPro	IPR009056. Cyt_c_monohaem. IPR019556. PQQ-dependent_C. IPR019551. PQQ-dependent_N. IPR018391. PQQ_beta_propeller_repeat. IPR017512. PQQ_MeOH/EtOH_DH. IPR002372. PQQ_repeat. IPR011047. Quino_AlcDH-like. IPR001479. Quinoprotein_DH_CS. [Graphical view]
Gene3D	G3DSA:1.10.760.10. Cytochrome_c_R. 1 hit. G3DSA:2.140.10.10. Quinoprotein_alc_DH-like. 1 hit.
Pfam	PF01011. PQQ. 6 hits. PF10535. PQQ_C. 1 hit. PF10527. PQQ_N. 1 hit. [Graphical view]
SMART	SM00564. PQQ. 5 hits. [Graphical view]
TIGRFAMs	TIGR03075. PQQ_enz_alc_DH. 1 hit.
PROSITE	PS00363. BACTERIAL_PQQ_1. 1 hit. PS00364. BACTERIAL_PQQ_2. 1 hit. PS51007. CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHET_ACEEU

Accession

Primary (citable) accession number: Q44002
Secondary accession number(s): O07952

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	September 22, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)