Reviewed, UniProtKB/Swiss-Prot Q43990 (SYK_ACIAD)

Names and origin

Protein names

Recommended name:
    Lysyl-tRNA synthetase
    EC=6.1.1.6
Alternative name(s):
    Lysine--tRNA ligase
      Short name=LysRS

Gene names

Name:	lysS
Ordered Locus Names:	ACIAD1069

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00252
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Lysyl-tRNA synthetase HAMAP MF_00252		PRO_0000152592
Sites
	Metal binding	418	1	Magnesium 1 By similarity
	Metal binding	425	1	Magnesium 1 By similarity
	Metal binding	425	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43990-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 95ED1AA43DC3D2F6	FASTA	509	58,079
	10 20 30 40 50 60 MAQPNVQSTS EPILSENDLI AQRHAKLKQI QDKAKETGKS VWPNTFKREH YAADLQEQFK 70 80 90 100 110 120 DIDKAQIEGS DKTYVKVAGR VMLNRGSFMV IQDMTGRIQL YVDRKGLPAD TLETIKSLDL 130 140 150 160 170 180 GDIIAAEGYI GRSGKGDLYV HLEGFELLTK SLRPLPDKFH GLTDTEAKYR KRYLDLIVNE 190 200 210 220 230 240 ETRKTFEIRA QVVAGIRAFL TNQRFMEVET PMMHVIPGGA SAQPFVTHHN ALDMELYLRI 250 260 270 280 290 300 APELYLKRLV VGGFERVFEI NRNFRNEGVS TRHNPEFTMI EFYQAYADYK DLMQLTENML 310 320 330 340 350 360 EKLALDILGT TDVPYGDEVY SFKGPFKKIS MFDAILEHNP DFTPENVNDR EFLAKFTQDV 370 380 390 400 410 420 LKEQVKPGFG LGKLQTIVFE ETVETKLRQP TFITEYPAET SPLARRNDDN PHITDRFEFF 430 440 450 460 470 480 IGGRELANGF SELNDPIDQA ERFQAQVAEK DAGDDEAMHY DADFIEALEY GLPPTAGQGI 490 500 GIDRLVMIFA NAPSIRDVLL FPHMRRKDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a putative periplasmic Mn superoxide dismutase from Acinetobacter calcoaceticus ADP1." Geissdoerfer W., Ratajczak A., Hillen W. Gene 186:305-308(1997) [PubMed: 9074511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46863 Genomic DNA. Translation: CAA86924.1. CR543861 Genomic DNA. Translation: CAG67956.1.
RefSeq	YP_045778.1.
3D structure databases
HSSP	HSSP built from PDB template 1BBW based on UniProtKB P13030.
ModBase	Search...
Genome annotation databases
GeneID	2878610.
GenomeReviews	Gene locus ACIAD1069 in contig CR543861_GR.
KEGG	aci:ACIAD1069.
NMPDR	fig|62977.3.peg.1217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q43990.
OMA	Q43990. ANEESRH.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD1069-MON.
Family and domain databases
HAMAP	MF_00252. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002313. Lys-tRNA-synth_II. IPR018149. Lys-tRNA-synth_II_C. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF4. tRNA-synt_lys_2. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
TIGRFAMs	TIGR00499. lysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK_ACIAD

Accession

Primary (citable) accession number: Q43990

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)