Reviewed, UniProtKB/Swiss-Prot Q43963 (TRMD_ACIAD)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	ACIAD3311

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060313
Regions
	Region	137 – 142	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	117	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43963-1 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: 215139F2B65A195C	FASTA	249	28,287
	10 20 30 40 50 60 MFFAVITLFP EMFEAITAYG ISGRATKRQI ATVSCINPRD FAEGNYKRVD ERPFGGGPGM 70 80 90 100 110 120 VMMAEPLAKA IIHAKQLAEQ AGCVHAPVVY MSPQGKTLNE HAVQQFVDYD GLIVLCGRYE 130 140 150 160 170 180 GVDERLIQHY VDQEWSIGDY VLSGGELPAM VLLDSIIRRL PDAMSDEQSH IQDSFVDGLL 190 200 210 220 230 240 DCPQYTKPDH FEGLDVPEVL KSGHHANIEK WRFLQRYQRT LDRRPELVEK VTLTKQQRKW LTFLDDSKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Characterization of lipase-deficient mutants of Acinetobacter calcoaceticus BD413: identification of a periplasmic lipase chaperone essential for the production of extracellular lipase." Kok R.G., Thor J.J., Nugteren-Roodzant I.M., Vosman B., Hellingwerf K.J. J. Bacteriol. 177:3295-3307(1995) [PubMed: 7768830] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 132-249.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG69984.1. X80800 Genomic DNA. Translation: CAA56781.1.
PIR	S61928.
RefSeq	YP_047806.1.
3D structure databases
HSSP	HSSP built from PDB template 1UAJ based on UniProtKB P43912.
ModBase	Search...
Protein-protein interaction databases
STRING	Q43963.
Genome annotation databases
GeneID	2880730.
GenomeReviews	Gene locus ACIAD3311 in contig CR543861_GR.
KEGG	aci:ACIAD3311.
NMPDR	fig|62977.3.peg.2999.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q43963.
OMA	HRSVDDT.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD3311-MON.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_ACIAD

Accession

Primary (citable) accession number: Q43963
Secondary accession number(s): Q6F7I1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 31, 2004
Last modified:	November 3, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)