Reviewed, UniProtKB/Swiss-Prot Q43914 (DLHH_AZOBR)

Names and origin

Protein names	Recommended name:     Putative carboxymethylenebutenolidase     EC=3.1.1.45 Alternative name(s):     Dienelactone hydrolase       Short name=DLH
Organism	Azospirillum brasilense
Taxonomic identifier	192 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Azospirillum

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate.
Sequence similarities	Belongs to the dienelactone hydrolase family.

Ontologies

Keywords
Molecular function	Hydrolase
Gene Ontology (GO)
Molecular function	carboxymethylenebutenolidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Putative carboxymethylenebutenolidase		PRO_0000161577
Sites
	Active site	169	1	By similarity
	Active site	200	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43914-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B7B76E567F9E0D1E	FASTA	231	24,866
	10 20 30 40 50 60 MTDISIPAGD DGSFSAYVAK PAGGGPAPGL VVIQEIFGVN QVMRDLCDAF AAQGWLAVCP 70 80 90 100 110 120 DLFWRQEPGV QITDKTQEEW NRAFALMNGM DQDKRWTTSR PPCRGCARIR IAPAKRVGRL 130 140 150 160 170 180 LLGRRLAFMM AARSDSDANV SYYGVGLDGL VGEAASITKP LLMHIAEKDQ FVPAEAREKV 190 200 210 220 230 LAAVKGNPNV TAHVYPGVDH AFARAGGAHF EPEAAELANG RTAAFFKQHL G

References

[1]	"Identification and sequencing of pyrG, the CTP synthetase gene of Azospirillum brasilense Sp7." Zimmer W., Hundeshagen B. FEMS Microbiol. Lett. 115:273-278(1994) [PubMed: 8138139] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29145 / Sp7 / DSM 1690 / IMET 11303.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X67216 Genomic DNA. Translation: CAA47657.1.
PIR	I39497.
3D structure databases
HSSP	HSSP built from PDB template 1DIN based on UniProtKB P11453.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.1.45. 1315.
Family and domain databases
InterPro	IPR002925. Dienelactn_hydro. [Graphical view]
Pfam	PF01738. DLH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DLHH_AZOBR

Accession

Primary (citable) accession number: Q43914

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 20, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)