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Q43907 (GYRB_ACIBA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA gyrase subunit B

EC=5.99.1.3
Gene names
Name:gyrB
OrganismAcinetobacter baumannii
Taxonomic identifier470 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length388 AA.
Sequence statusFragment.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01898

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01898

Subunit structure

Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.

Subcellular location

Cytoplasm Potential HAMAP MF_01898.

Sequence similarities

Belongs to the type II topoisomerase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionIsomerase
Topoisomerase
Gene Ontology (GO)
   Biological processDNA topological change

Inferred from electronic annotation. Source: InterPro

   Cellular componentchromosome

Inferred from electronic annotation. Source: InterPro

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase (ATP-hydrolyzing) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›388›388DNA gyrase subunit B HAMAP MF_01898
PRO_0000145274

Experimental info

Sequence conflict1101Y → F in BAA11153. Ref.2
Non-terminal residue11
Non-terminal residue3881

Sequences

Sequence LengthMass (Da)Tools
Q43907 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: 826C99662039D591

FASTA38843,111
        10         20         30         40         50         60 
DNSYKVSGGL HGVGVSVVNA LSSKLHLTIY RAGQIHEQEY HHGDPQYPLR VIGETDNTGT 

        70         80         90        100        110        120 
TVRFWPSAET FSQTIFNVEI LARRLRELSF LNAGVRIVLR DERINLEHVY DYEGGLSEFV 

       130        140        150        160        170        180 
KYINEGKNHL NEIFHFTADA DNGIAVEVAL QWNDSYQENV RCFTNNIPQK DGGTHLAGFR 

       190        200        210        220        230        240 
AALTRGLNQY LENENILKKE KVNVTGDDAR EGLTAIISVK VPDPKFSSQT KEKLVSSEVK 

       250        260        270        280        290        300 
PAVEQAMNKE FSAYLLENPQ AAKSIAGKII DAARARDAAR KAREMTRRKS ALDIAGLPGK 

       310        320        330        340        350        360 
LADCQEKDPA LSELYLVEGD SAGGSAKQGR NRKMQAILPL KGKILNVERA RFDKMISSQE 

       370        380 
VGTLITALGC GIGREEYNPD KLRYHKII 

« Hide

References

[1]"Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization."
Yamamoto S., Bouvet P.J.M., Harayama S.
Int. J. Syst. Bacteriol. 49:87-95(1999) [PubMed: 10028249] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 17945 / CIP 70.22, ATCC 17961 / CIP 70.32 / B55 and ATCC 19606 / NCTC 12156 / CIP 70.34.
[2]"Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products."
Yamamoto S., Harayama S.
Int. J. Syst. Bacteriol. 46:506-511(1996) [PubMed: 8934907] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-118 AND 289-388.
Strain: ATCC 19606 / NCTC 12156 / CIP 70.34.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB008684 Genomic DNA. Translation: BAA75401.1.
AB008700 Genomic DNA. Translation: BAA75417.1.
AB008699 Genomic DNA. Translation: BAA75416.1.
D73428 Genomic DNA. Translation: BAA11153.1.
D73413 Genomic DNA. Translation: BAA11138.1.
PIRT43897.

3D structure databases

ProteinModelPortalQ43907.
SMRQ43907. Positions 1-286.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

HAMAPMF_01898. GyrB.
[Tree]
InterProIPR003594. ATPase-like_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013759. Topo_IIA_B/N_ab.
IPR013506. Topo_IIA_bsu_dom2.
IPR013760. Topo_IIA_cen.
IPR018522. TopoIIA_CS.
[Graphical view]
Gene3DG3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
PfamPF00204. DNA_gyraseB. 1 hit.
[Graphical view]
PRINTSPR00418. TPI2FAMILY.
SMARTSM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMSSF55874. ATP_bd_ATPase. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
SSF56719. Topo_IIA_cen. 1 hit.
PROSITEPS00177. TOPOISOMERASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGYRB_ACIBA
AccessionPrimary (citable) accession number: Q43907
Secondary accession number(s): Q59090, Q9ZA10
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 11, 2001
Last modified: January 25, 2012
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families