Q43907 (GYRB_ACIBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit B
EC=5.99.1.3

Gene names

Name:

gyrB

Organism

Acinetobacter baumannii

Taxonomic identifier

470 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	388 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01898
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01898
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01898.
Sequence similarities	Belongs to the type II topoisomerase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›388	›388	DNA gyrase subunit B HAMAP MF_01898		PRO_0000145274
Experimental info
	Sequence conflict	110	1	Y → F in BAA11153. Ref.2
	Non-terminal residue	1	1
	Non-terminal residue	388	1

Sequences

	Sequence		Length	Mass (Da)
	Q43907 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 826C99662039D591	FASTA	388	43,111
	10 20 30 40 50 60 DNSYKVSGGL HGVGVSVVNA LSSKLHLTIY RAGQIHEQEY HHGDPQYPLR VIGETDNTGT 70 80 90 100 110 120 TVRFWPSAET FSQTIFNVEI LARRLRELSF LNAGVRIVLR DERINLEHVY DYEGGLSEFV 130 140 150 160 170 180 KYINEGKNHL NEIFHFTADA DNGIAVEVAL QWNDSYQENV RCFTNNIPQK DGGTHLAGFR 190 200 210 220 230 240 AALTRGLNQY LENENILKKE KVNVTGDDAR EGLTAIISVK VPDPKFSSQT KEKLVSSEVK 250 260 270 280 290 300 PAVEQAMNKE FSAYLLENPQ AAKSIAGKII DAARARDAAR KAREMTRRKS ALDIAGLPGK 310 320 330 340 350 360 LADCQEKDPA LSELYLVEGD SAGGSAKQGR NRKMQAILPL KGKILNVERA RFDKMISSQE 370 380 VGTLITALGC GIGREEYNPD KLRYHKII

References

[1]	"Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization." Yamamoto S., Bouvet P.J.M., Harayama S. Int. J. Syst. Bacteriol. 49:87-95(1999) [PubMed: 10028249] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 17945 / CIP 70.22, ATCC 17961 / CIP 70.32 / B55 and ATCC 19606 / NCTC 12156 / CIP 70.34.
[2]	"Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products." Yamamoto S., Harayama S. Int. J. Syst. Bacteriol. 46:506-511(1996) [PubMed: 8934907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-118 AND 289-388. Strain: ATCC 19606 / NCTC 12156 / CIP 70.34.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB008684 Genomic DNA. Translation: BAA75401.1. AB008700 Genomic DNA. Translation: BAA75417.1. AB008699 Genomic DNA. Translation: BAA75416.1. D73428 Genomic DNA. Translation: BAA11153.1. D73413 Genomic DNA. Translation: BAA11138.1.
PIR	T43897.
3D structure databases
ProteinModelPortal	Q43907.
SMR	Q43907. Positions 1-286.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01898. GyrB. [Tree]
InterPro	IPR003594. ATPase-like_ATP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR013759. Topo_IIA_B/N_ab. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
Pfam	PF00204. DNA_gyraseB. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00433. TOP2c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF56719. Topo_IIA_cen. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRB_ACIBA

Accession

Primary (citable) accession number: Q43907
Secondary accession number(s): Q59090, Q9ZA10

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 11, 2001
Last modified:	January 25, 2012
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program