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Protein

Aminoglycoside N(6')-acetyltransferase type 1

Gene
N/A
Organism
Acinetobacter baumannii
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of an acetyl group from acetyl-CoA to the 6'-amino group of aminoglycoside molecules conferring resistance to antibiotics containing the purpurosamine ring including amikacin, kanamycin, tobramycin and netilmicin.1 Publication

Catalytic activityi

Acetyl-CoA + kanamycin-B = CoA + N(6')-acetylkanamycin-B.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221SubstrateBy similarity
Binding sitei25 – 251SubstrateBy similarity
Binding sitei66 – 661SubstrateBy similarity
Binding sitei79 – 791SubstrateBy similarity
Binding sitei115 – 1151SubstrateBy similarity
Binding sitei120 – 1201Acetyl-CoABy similarity
Binding sitei136 – 1361SubstrateBy similarity

GO - Molecular functioni

  • aminoglycoside 6'-N-acetyltransferase activity Source: UniProtKB

GO - Biological processi

  • response to antibiotic Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoglycoside N(6')-acetyltransferase type 11 Publication (EC:2.3.1.821 Publication)
Alternative name(s):
AAC(6')-IhImported
Aminoglycoside resistance proteinBy similarity
Encoded oniPlasmid pIP18581 Publication
OrganismiAcinetobacter baumannii
Taxonomic identifieri470 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 146146Aminoglycoside N(6')-acetyltransferase type 1PRO_0000416833Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

Secondary structure

1
146
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 54Combined sources
Helixi11 – 2111Combined sources
Helixi26 – 3712Combined sources
Beta strandi42 – 487Combined sources
Beta strandi54 – 6310Combined sources
Beta strandi72 – 8312Combined sources
Helixi85 – 906Combined sources
Helixi92 – 10514Combined sources
Turni106 – 1083Combined sources
Beta strandi111 – 1177Combined sources
Helixi121 – 1299Combined sources
Beta strandi133 – 14412Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4E8OX-ray2.14A/B1-146[»]
ProteinModelPortaliQ43899.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 146146N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni81 – 833Acetyl-CoA bindingBy similarity

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK18816.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR024170. Aminoglycoside_N6-AcTrfrase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000452. 6-N-acetyltransf. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q43899-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIMPISESQ LSDWLALRCL LWPDHEDVHL QEMRQLITQA HRLQLLAYTD
60 70 80 90 100
TQQAIAMLEA SIRYEYVNGT QTSPVAFLEG IFVLPEYRRS GIATGLVQQV
110 120 130 140
EIWAKQFACT EFASDAALDN QISHAMHQAL GFHETERVVY FKKNIG
Length:146
Mass (Da):16,709
Last modified:November 1, 1996 - v1
Checksum:iFAA8FBF093B7F1EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29044 Genomic DNA. Translation: AAC41391.1.
PIRiI39503.

Genome annotation databases

KEGGiag:AAC41391.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29044 Genomic DNA. Translation: AAC41391.1.
PIRiI39503.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4E8OX-ray2.14A/B1-146[»]
ProteinModelPortaliQ43899.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAC41391.

Phylogenomic databases

KOiK18816.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR024170. Aminoglycoside_N6-AcTrfrase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000452. 6-N-acetyltransf. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of the chromosomal aac(6')-Ij gene of Acinetobacter sp. 13 and the aac(6')-Ih plasmid gene of Acinetobacter baumannii."
    Lambert T., Gerbaud G., Courvalin P.
    Antimicrob. Agents Chemother. 38:1883-1889(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY.
    Strain: BM2686Imported.
    Plasmid: pIP1858

Entry informationi

Entry nameiAAC6_ACIBA
AccessioniPrimary (citable) accession number: Q43899
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: November 1, 1996
Last modified: November 11, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.