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Protein

Aminoglycoside N(6')-acetyltransferase type 1

Gene
N/A
Organism
Acinetobacter baumannii
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of an acetyl group from acetyl-CoA to the 6'-amino group of aminoglycoside molecules conferring resistance to antibiotics containing the purpurosamine ring including amikacin, kanamycin, tobramycin and netilmicin.1 Publication

Catalytic activityi

Acetyl-CoA + kanamycin-B = CoA + N(6')-acetylkanamycin-B.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22SubstrateBy similarity1
Binding sitei25SubstrateBy similarity1
Binding sitei66SubstrateBy similarity1
Binding sitei79SubstrateBy similarity1
Binding sitei115SubstrateBy similarity1
Binding sitei120Acetyl-CoABy similarity1
Binding sitei136SubstrateBy similarity1

GO - Molecular functioni

  • aminoglycoside 6'-N-acetyltransferase activity Source: UniProtKB

GO - Biological processi

  • response to antibiotic Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoglycoside N(6')-acetyltransferase type 11 Publication (EC:2.3.1.821 Publication)
Alternative name(s):
AAC(6')-IhImported
Aminoglycoside resistance proteinBy similarity
Encoded oniPlasmid pIP18581 Publication
OrganismiAcinetobacter baumannii
Taxonomic identifieri470 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004168331 – 146Aminoglycoside N(6')-acetyltransferase type 1Add BLAST146

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

Secondary structure

1146
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi11 – 21Combined sources11
Helixi26 – 37Combined sources12
Beta strandi42 – 48Combined sources7
Beta strandi54 – 63Combined sources10
Beta strandi72 – 83Combined sources12
Helixi85 – 90Combined sources6
Helixi92 – 105Combined sources14
Turni106 – 108Combined sources3
Beta strandi111 – 117Combined sources7
Helixi121 – 129Combined sources9
Beta strandi133 – 144Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4E8OX-ray2.14A/B1-146[»]
ProteinModelPortaliQ43899.
SMRiQ43899.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 146N-acetyltransferasePROSITE-ProRule annotationAdd BLAST146

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni81 – 83Acetyl-CoA bindingBy similarity3

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK18816.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR024170. Aminoglycoside_N6-AcTrfrase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000452. 6-N-acetyltransf. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q43899-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIMPISESQ LSDWLALRCL LWPDHEDVHL QEMRQLITQA HRLQLLAYTD
60 70 80 90 100
TQQAIAMLEA SIRYEYVNGT QTSPVAFLEG IFVLPEYRRS GIATGLVQQV
110 120 130 140
EIWAKQFACT EFASDAALDN QISHAMHQAL GFHETERVVY FKKNIG
Length:146
Mass (Da):16,709
Last modified:November 1, 1996 - v1
Checksum:iFAA8FBF093B7F1EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29044 Genomic DNA. Translation: AAC41391.1.
PIRiI39503.
RefSeqiWP_016541245.1. NG_047297.1.

Genome annotation databases

GeneIDi23000000.
KEGGiag:AAC41391.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29044 Genomic DNA. Translation: AAC41391.1.
PIRiI39503.
RefSeqiWP_016541245.1. NG_047297.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4E8OX-ray2.14A/B1-146[»]
ProteinModelPortaliQ43899.
SMRiQ43899.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23000000.
KEGGiag:AAC41391.

Phylogenomic databases

KOiK18816.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR024170. Aminoglycoside_N6-AcTrfrase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000452. 6-N-acetyltransf. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAAC6_ACIBA
AccessioniPrimary (citable) accession number: Q43899
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.