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Q43880

- THER_ALIAC

UniProt

Q43880 - THER_ALIAC

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Protein

Thermolysin

Gene
N/A
Organism
Alicyclobacillus acidocaldarius (Bacillus acidocaldarius)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+Note: Binds 4 Ca(2+) ions per subunit.
  • Zn2+Note: Binds 1 zinc ion per subunit.

Temperature dependencei

Thermostable.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi287 – 2871Calcium 1By similarity
Metal bindingi289 – 2891Calcium 1By similarity
Metal bindingi291 – 2911Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi368 – 3681Calcium 2By similarity
Metal bindingi372 – 3721Zinc; catalyticPROSITE-ProRule annotation
Active sitei373 – 3731PROSITE-ProRule annotation
Metal bindingi376 – 3761Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi396 – 3961Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi407 – 4071Calcium 2By similarity
Metal bindingi407 – 4071Calcium 3By similarity
Metal bindingi413 – 4131Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi415 – 4151Calcium 2By similarity
Metal bindingi415 – 4151Calcium 3By similarity
Metal bindingi417 – 4171Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi420 – 4201Calcium 2By similarity
Metal bindingi420 – 4201Calcium 3By similarity
Metal bindingi423 – 4231Calcium 4; via carbonyl oxygenBy similarity
Metal bindingi424 – 4241Calcium 4By similarity
Metal bindingi427 – 4271Calcium 4; via carbonyl oxygenBy similarity
Metal bindingi430 – 4301Calcium 4By similarity
Active sitei461 – 4611Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermolysin (EC:3.4.24.27)
Alternative name(s):
Thermostable neutral proteinase
OrganismiAlicyclobacillus acidocaldarius (Bacillus acidocaldarius)
Taxonomic identifieri1388 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesAlicyclobacillaceaeAlicyclobacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Propeptidei26 – 228203Activation peptidePRO_0000028584Add
BLAST
Chaini229 – 546318ThermolysinPRO_0000028585Add
BLAST

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ43880.
SMRiQ43880. Positions 228-545.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43880-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKRAMLGAI GLAFGLMAWP FGASAKEKSM VWNEQWKTPS FVSGSLLKGE
60 70 80 90 100
DAPEELVYRY LDQEKNTFQL GGQARERLSL IGKQTDELGH TVMRFEQRYR
110 120 130 140 150
GIPVYGAVLV AHVNDGELSS LSGTLIPNLD KRTLKTEAAI SIQQAEMIAK
160 170 180 190 200
QDVADAVTKE RPAAEEGKPT RLVIYPDGET PRLAYEVNVR FLTPVPGNWI
210 220 230 240 250
YMIDAADGKV LNKWNQMDEA KPGGGQPVAG TSTVGVGRGV LGDQKYINTT
260 270 280 290 300
YSSYYGYYYL QDNTRGSGIF TYDGRNRTVL PGSLWADGDN QFFASYDAAA
310 320 330 340 350
VDAHYYAGVV YDYYKNVHGR LSYDGSNAAI RSTVHYGRGY NNAFWNGSQM
360 370 380 390 400
VYGDGDGQTF LPFSGGIDVV GHELTHAVTD YTAGLVYQNE SGAINEAMSD
410 420 430 440 450
IFGTLVEFYA NRNPDWEIGE DIYTPGIAGD ALRSMSDPAK YGDPDHYSKR
460 470 480 490 500
YTGTQDNGGV HTNSGIINKA AYLLSQGGVH YGVSVTGIGR DKMGKIFYRA
510 520 530 540
LVYYLTPTSN FSQLRAACVQ AAADLYGSTS QEVNSVKQAF NAVGVY
Length:546
Mass (Da):59,770
Last modified:November 1, 1996 - v1
Checksum:i3AC33A611BD2B071
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07824 Genomic DNA. Translation: AAC43402.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07824 Genomic DNA. Translation: AAC43402.1 .

3D structure databases

ProteinModelPortali Q43880.
SMRi Q43880. Positions 228-545.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M04.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of the neutral protease-encoding gene from a thermophilic strain of Bacillus sp."
    Vecerek B., Kyslik P.
    Gene 158:147-148(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BT1.

Entry informationi

Entry nameiTHER_ALIAC
AccessioniPrimary (citable) accession number: Q43880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3