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Q43880

- THER_ALIAC

UniProt

Q43880 - THER_ALIAC

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Protein
Thermolysin
Gene
N/A
Organism
Alicyclobacillus acidocaldarius (Bacillus acidocaldarius)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe.

Cofactori

Binds 4 calcium ions per subunit.
Binds 1 zinc ion per subunit.

Temperature dependencei

Thermostable.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi287 – 2871Calcium 1 By similarity
Metal bindingi289 – 2891Calcium 1 By similarity
Metal bindingi291 – 2911Calcium 1; via carbonyl oxygen By similarity
Metal bindingi368 – 3681Calcium 2 By similarity
Metal bindingi372 – 3721Zinc; catalytic By similarity
Active sitei373 – 3731 By similarity
Metal bindingi376 – 3761Zinc; catalytic By similarity
Metal bindingi396 – 3961Zinc; catalytic By similarity
Metal bindingi407 – 4071Calcium 2 By similarity
Metal bindingi407 – 4071Calcium 3 By similarity
Metal bindingi413 – 4131Calcium 3; via carbonyl oxygen By similarity
Metal bindingi415 – 4151Calcium 2 By similarity
Metal bindingi415 – 4151Calcium 3 By similarity
Metal bindingi417 – 4171Calcium 2; via carbonyl oxygen By similarity
Metal bindingi420 – 4201Calcium 2 By similarity
Metal bindingi420 – 4201Calcium 3 By similarity
Metal bindingi423 – 4231Calcium 4; via carbonyl oxygen By similarity
Metal bindingi424 – 4241Calcium 4 By similarity
Metal bindingi427 – 4271Calcium 4; via carbonyl oxygen By similarity
Metal bindingi430 – 4301Calcium 4 By similarity
Active sitei461 – 4611Proton donor By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermolysin (EC:3.4.24.27)
Alternative name(s):
Thermostable neutral proteinase
OrganismiAlicyclobacillus acidocaldarius (Bacillus acidocaldarius)
Taxonomic identifieri1388 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesAlicyclobacillaceaeAlicyclobacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed prediction
Add
BLAST
Propeptidei26 – 228203Activation peptide
PRO_0000028584Add
BLAST
Chaini229 – 546318Thermolysin
PRO_0000028585Add
BLAST

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ43880.
SMRiQ43880. Positions 228-545.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43880-1 [UniParc]FASTAAdd to Basket

« Hide

MNKRAMLGAI GLAFGLMAWP FGASAKEKSM VWNEQWKTPS FVSGSLLKGE    50
DAPEELVYRY LDQEKNTFQL GGQARERLSL IGKQTDELGH TVMRFEQRYR 100
GIPVYGAVLV AHVNDGELSS LSGTLIPNLD KRTLKTEAAI SIQQAEMIAK 150
QDVADAVTKE RPAAEEGKPT RLVIYPDGET PRLAYEVNVR FLTPVPGNWI 200
YMIDAADGKV LNKWNQMDEA KPGGGQPVAG TSTVGVGRGV LGDQKYINTT 250
YSSYYGYYYL QDNTRGSGIF TYDGRNRTVL PGSLWADGDN QFFASYDAAA 300
VDAHYYAGVV YDYYKNVHGR LSYDGSNAAI RSTVHYGRGY NNAFWNGSQM 350
VYGDGDGQTF LPFSGGIDVV GHELTHAVTD YTAGLVYQNE SGAINEAMSD 400
IFGTLVEFYA NRNPDWEIGE DIYTPGIAGD ALRSMSDPAK YGDPDHYSKR 450
YTGTQDNGGV HTNSGIINKA AYLLSQGGVH YGVSVTGIGR DKMGKIFYRA 500
LVYYLTPTSN FSQLRAACVQ AAADLYGSTS QEVNSVKQAF NAVGVY 546
Length:546
Mass (Da):59,770
Last modified:November 1, 1996 - v1
Checksum:i3AC33A611BD2B071
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U07824 Genomic DNA. Translation: AAC43402.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U07824 Genomic DNA. Translation: AAC43402.1 .

3D structure databases

ProteinModelPortali Q43880.
SMRi Q43880. Positions 228-545.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M04.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of the neutral protease-encoding gene from a thermophilic strain of Bacillus sp."
    Vecerek B., Kyslik P.
    Gene 158:147-148(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BT1.

Entry informationi

Entry nameiTHER_ALIAC
AccessioniPrimary (citable) accession number: Q43880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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