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Protein

Peroxidase 73

Gene

PER73

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei63 – 631Transition state stabilizerPROSITE-ProRule annotation
Active sitei67 – 671Proton acceptorPROSITE-ProRule annotation
Metal bindingi68 – 681Calcium 1PROSITE-ProRule annotation
Metal bindingi71 – 711Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi73 – 731Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi75 – 751Calcium 1PROSITE-ProRule annotation
Metal bindingi77 – 771Calcium 1PROSITE-ProRule annotation
Binding sitei167 – 1671Substrate; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi197 – 1971Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi198 – 1981Calcium 2PROSITE-ProRule annotation
Metal bindingi249 – 2491Calcium 2PROSITE-ProRule annotation
Metal bindingi252 – 2521Calcium 2PROSITE-ProRule annotation
Metal bindingi257 – 2571Calcium 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G67400-MONOMER.

Protein family/group databases

PeroxiBasei239. AtPrx73.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase 73 (EC:1.11.1.7)
Short name:
Atperox P73
Alternative name(s):
ATP10a
PRXR11
Gene namesi
Name:PER73
Synonyms:P73
Ordered Locus Names:At5g67400
ORF Names:K8K14.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G67400.

Subcellular locationi

  • Secreted PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 329304Peroxidase 73PRO_0000023738Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 119PROSITE-ProRule annotation
Disulfide bondi69 ↔ 74PROSITE-ProRule annotation
Disulfide bondi125 ↔ 325PROSITE-ProRule annotation
Disulfide bondi204 ↔ 236PROSITE-ProRule annotation
Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ43873.
PRIDEiQ43873.

Expressioni

Tissue specificityi

Expressed in the whole plant, with the highest expression in roots.

Inductioni

Up-regulated transiently by a cold treatment.1 Publication

Gene expression databases

ExpressionAtlasiQ43873. differential.
GenevisibleiQ43873. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G67400.1.

Structurei

3D structure databases

ProteinModelPortaliQ43873.
SMRiQ43873. Positions 26-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IJ72. Eukaryota.
ENOG4111FDS. LUCA.
HOGENOMiHOG000237556.
InParanoidiQ43873.
KOiK00430.
OMAiHAFDNQY.
PhylomeDBiQ43873.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43873-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARFSLVVVV TLSLAISMFP DTTTAQLKTN FYGNSCPNVE QIVKKVVQEK
60 70 80 90 100
IKQTFVTIPA TLRLFFHDCF VNGCDASVMI QSTPTNKAEK DHPDNISLAG
110 120 130 140 150
DGFDVVIKAK KALDAIPSCK NKVSCADILA LATRDVVVAA KGPSYAVELG
160 170 180 190 200
RFDGLVSTAA SVNGNLPGPN NKVTELNKLF AKNKLTQEDM IALSAAHTLG
210 220 230 240 250
FAHCGKVFNR IYNFNLTHAV DPTLNKAYAK ELQLACPKTV DPRIAINMDP
260 270 280 290 300
TTPRQFDNIY FKNLQQGKGL FTSDQVLFTD GRSKPTVNDW AKNSVAFNKA
310 320
FVTAMTKLGR VGVKTRRNGN IRRDCGAFN
Length:329
Mass (Da):35,927
Last modified:November 1, 1996 - v1
Checksum:i20869E19AD16E987
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98323 mRNA. Translation: CAA66967.1.
X98928 mRNA. Translation: CAA67428.1.
AB007645 Genomic DNA. Translation: BAB09025.1.
CP002688 Genomic DNA. Translation: AED98339.1.
RefSeqiNP_201541.1. NM_126140.2.
UniGeneiAt.66740.
At.95.

Genome annotation databases

EnsemblPlantsiAT5G67400.1; AT5G67400.1; AT5G67400.
GeneIDi836876.
GrameneiAT5G67400.1; AT5G67400.1; AT5G67400.
KEGGiath:AT5G67400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98323 mRNA. Translation: CAA66967.1.
X98928 mRNA. Translation: CAA67428.1.
AB007645 Genomic DNA. Translation: BAB09025.1.
CP002688 Genomic DNA. Translation: AED98339.1.
RefSeqiNP_201541.1. NM_126140.2.
UniGeneiAt.66740.
At.95.

3D structure databases

ProteinModelPortaliQ43873.
SMRiQ43873. Positions 26-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G67400.1.

Protein family/group databases

PeroxiBasei239. AtPrx73.

Proteomic databases

PaxDbiQ43873.
PRIDEiQ43873.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G67400.1; AT5G67400.1; AT5G67400.
GeneIDi836876.
GrameneiAT5G67400.1; AT5G67400.1; AT5G67400.
KEGGiath:AT5G67400.

Organism-specific databases

TAIRiAT5G67400.

Phylogenomic databases

eggNOGiENOG410IJ72. Eukaryota.
ENOG4111FDS. LUCA.
HOGENOMiHOG000237556.
InParanoidiQ43873.
KOiK00430.
OMAiHAFDNQY.
PhylomeDBiQ43873.

Enzyme and pathway databases

BioCyciARA:AT5G67400-MONOMER.

Miscellaneous databases

PROiQ43873.

Gene expression databases

ExpressionAtlasiQ43873. differential.
GenevisibleiQ43873. AT.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Eleven cDNA clones from Arabidopsis thaliana encoding isoperoxidases."
    Capelli N., Tognolli M., Flach J., Overney S., Penel C., Greppin H., Simon P.
    Plant Gene Register PGR96-066
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Flower, Leaf, Root, Silique and Stem.
  2. "From expressed sequence tags to structure, function, evolution and expression of 28 ER-targeted Arabidopsis peroxidases."
    Welinder K.G., Jespersen H.M., Kjaersgaard I.V.H., Justesen A.F., Oestergaard L., Abelskov A.K., Jensen R.B., Hansen L.N., Rasmussen S.K.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Computational analyses and annotations of the Arabidopsis peroxidase gene family."
    Oestergaard L., Pedersen A.G., Jespersen H.M., Brunak S., Welinder K.G.
    FEBS Lett. 433:98-102(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: cv. Columbia.
  6. "Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway."
    Fowler S., Thomashow M.F.
    Plant Cell 14:1675-1690(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: cv. Columbia.
  7. "Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana."
    Tognolli M., Penel C., Greppin H., Simon P.
    Gene 288:129-138(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.

Entry informationi

Entry nameiPER73_ARATH
AccessioniPrimary (citable) accession number: Q43873
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.