Reviewed, UniProtKB/Swiss-Prot Q43872 (PER64_ARATH)

Names and origin

Protein names

Recommended name:
    Peroxidase 64
      Short name=Atperox P64
    EC=1.11.1.7
Alternative name(s):
    PRXR4
    ATP17a

Gene names

Name:	PER64
Synonyms:	P64
Ordered Locus Names:	At5g42180
ORF Names:	MJC20.29

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity. Binds 2 calcium ions per subunit By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in the whole plant, but preferentially in roots.
Induction	Pathogen and elicitor-induced. Up-regulated transiently by a cold treatment. Ref.6 Ref.7
Miscellaneous	There are 73 peroxidase genes in A.thaliana.
Sequence similarities	Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plant-type cell wall Inferred from direct assay. Source: TAIR
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 317	295	Peroxidase 64		PRO_0000023729
Sites
	Active site	63	1	Proton acceptor By similarity
	Metal binding	64	1	Calcium 1 By similarity
	Metal binding	67	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	69	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	71	1	Calcium 1 By similarity
	Metal binding	73	1	Calcium 1 By similarity
	Metal binding	188	1	Iron (heme axial ligand) By similarity
	Metal binding	189	1	Calcium 2 By similarity
	Metal binding	241	1	Calcium 2 By similarity
	Metal binding	243	1	Calcium 2 By similarity
	Metal binding	248	1	Calcium 2 By similarity
	Binding site	158	1	Substrate; via carbonyl oxygen By similarity
	Site	59	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	163	1	N-linked (GlcNAc...) Potential
	Disulfide bond	32 ↔ 111		By similarity
	Disulfide bond	65 ↔ 70		By similarity
	Disulfide bond	117 ↔ 313		By similarity
	Disulfide bond	195 ↔ 227		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43872-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 79536112BA918690	FASTA	317	34,706
	10 20 30 40 50 60 MNAHMLNLLV IVIFVVSFDV QALSPHYYDH TCPQADHIVT NAVKKAMSND QTVPAALLRM 70 80 90 100 110 120 HFHDCFVRGC DGSVLLDSKG KNKAEKDGPP NISLHAFYVI DNAKKALEEQ CPGIVSCADI 130 140 150 160 170 180 LSLAARDAVA LSGGPTWAVP KGRKDGRISK AIETRQLPAP TFNISQLRQN FGQRGLSMHD 190 200 210 220 230 240 LVALSGGHTL GFAHCSSFQN RLHKFNTQKE VDPTLNPSFA ARLEGVCPAH NTVKNAGSNM 250 260 270 280 290 300 DGTVTSFDNI YYKMLIQGKS LFSSDESLLA VPSTKKLVAK YANSNEEFER AFVKSMIKMS 310 SISGNGNEVR LNCRRVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Eleven cDNA clones from Arabidopsis thaliana encoding isoperoxidases." Capelli N., Tognolli M., Flach J., Overney S., Penel C., Greppin H., Simon P. Plant Gene Register PGR96-066 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"From expressed sequence tags to structure, function, evolution and expression of 28 ER-targeted Arabidopsis peroxidases." Welinder K.G., Jespersen H.M., Kjaersgaard I.V.H., Justesen A.F., Oestergaard L., Abelskov A.K., Jensen R.B., Hansen L.N., Rasmussen S.K. Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S. DNA Res. 6:183-195(1999) [PubMed: 10470850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Computational analyses and annotations of the Arabidopsis peroxidase gene family." Oestergaard L., Pedersen A.G., Jespersen H.M., Brunak S., Welinder K.G. FEBS Lett. 433:98-102(1998) [PubMed: 9738941] [Abstract] Cited for: CHARACTERIZATION. Strain: cv. Columbia.
[6]	"Isolation of putative defense-related genes from Arabidopsis thaliana and expression in fungal elicitor-treated cells." Trezzini G.F., Horrichs A., Somssich I.E. Plant Mol. Biol. 21:385-389(1993) [PubMed: 8425063] [Abstract] Cited for: INDUCTION. Strain: cv. Columbia.
[7]	"Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway." Fowler S., Thomashow M.F. Plant Cell 14:1675-1690(2002) [PubMed: 12172015] [Abstract] Cited for: INDUCTION. Strain: cv. Columbia.
[8]	"Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana." Tognolli M., Penel C., Greppin H., Simon P. Gene 288:129-138(2002) [PubMed: 12034502] [Abstract] Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X98316 mRNA. Translation: CAA66960.1. X99096 mRNA. Translation: CAA67550.1. AB017067 Genomic DNA. Translation: BAB08451.1. AY063962 mRNA. Translation: AAL36318.1. AY096403 mRNA. Translation: AAM20043.1.
IPI	IPI00517914.
RefSeq	NP_199033.1.
UniGene	At.23304
3D structure databases
HSSP	HSSP built from PDB template 1QGJ based on UniProtKB Q39034.
ModBase	Search...
Protein family/group databases
PeroxiBase	230. AtPrx64.
Proteomic databases
PRIDE	Q43872.
Genome annotation databases
GeneID	834223.
GenomeReviews	Gene locus AT5G42180 in contig BA000015_GR.
KEGG	ath:AT5G42180.
NMPDR	fig|3702.1.peg.25939.
Organism-specific databases
GeneFarm	1923. 61.
TAIR	At5g42180.
Phylogenomic databases
OMA	Q43872. CSSFQNR.
Enzyme and pathway databases
BRENDA	1.11.1.7. 302.
Gene expression databases
ArrayExpress	Q43872.
GermOnline	AT5G42180. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002016. Haem_peroxidase_pln/fun/bac. IPR000823. Peroxidase_pln. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00458. PEROXIDASE. PR00461. PLPEROXIDASE.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. False negative. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PER64_ARATH

Accession

Primary (citable) accession number: Q43872

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)