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Protein

Pectinesterase 1

Gene

PME1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Probable pectinesterase 50 (PME50), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Probable pectinesterase 15 (PME15), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Putative pectinesterase 14 (PME14), Pectinesterase (At3g10720), Probable pectinesterase 55 (PME55), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Pectinesterase 4 (PME4), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase (At3g14310), Pectinesterase/pectinesterase inhibitor 18 (PME18), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Pectinesterase 1 (PME1), Putative pectinesterase 63 (PME63), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Putative pectinesterase 10 (PME10), Pectinesterase (F14I3.7), Pectinesterase 2 (PME2), Probable pectinesterase 29 (PME29), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Probable pectinesterase 8 (PME8), Pectinesterase, Pectinesterase/pectinesterase inhibitor 3 (PME3), Pectinesterase 31 (PME31), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Putative pectinesterase 57 (PME57), Pectinesterase (At3g62170), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Pectinesterase (T27B3.30), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase QRT1 (QRT1), Putative pectinesterase 11 (PME11), Pectinesterase PPME1 (PPME1), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 52 (PME52), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Pectinesterase (AXX17_AT1G48300), Pectinesterase (At1g53840), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase 30 (PME30), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Probable pectinesterase 68 (PME68), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Pectinesterase, Probable pectinesterase 67 (PME67), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Probable pectinesterase 56 (PME56), Pectinesterase, Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Probable pectinesterase 66 (PME66), Probable pectinesterase/pectinesterase inhibitor 6 (PME6)
  2. Pectate lyase (T26I12.20), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 18 (At4g24780), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Putative pectate lyase 14 (At4g13210), Pectate lyase (At3g55140), Probable pectate lyase 20 (At5g48900), Probable pectate lyase 6 (At2g02720), Pectate lyase (At1g14420), Pectate lyase (At3g01270), Pectate lyase (At2g02720), Probable pectate lyase 22 (At5g63180), Pectate lyase (T5E8_80), Pectate lyase (At3g01270), Pectate lyase (At3g55140), Putative pectate lyase 2 (At1g11920), Pectate lyase (At3g07010), Pectate lyase, Probable pectate lyase 8 (At3g07010), Probable pectate lyase 4 (At1g30350), Probable pectate lyase 19 (At5g15110), Pectate lyase (F11F8_12), Probable pectate lyase 13 (PMR6), Probable pectate lyase 3 (AT59), Probable pectate lyase 5 (At1g67750), Putative pectate lyase 21 (At5g55720), Pectate lyase (At4g13210), Probable pectate lyase 12 (At3g53190), Pectate lyase (At4g24780), Pectate lyase (At5g04310), Pectate lyase (At4g13710), Probable pectate lyase 15 (At4g13710), Pectate lyase (At3g53190), Pectate lyase (At3g01270), Putative pectate lyase 11 (At3g27400), Pectate lyase (At5g04310), Pectate lyase (At3g07010), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 1 (At1g04680), Probable pectate lyase 10 (At3g24670)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei355 – 3551SubstrateBy similarity
Binding sitei385 – 3851SubstrateBy similarity
Sitei407 – 4071Transition state stabilizerBy similarity
Active sitei408 – 4081Proton donorPROSITE-ProRule annotation
Active sitei429 – 4291NucleophilePROSITE-ProRule annotation
Binding sitei492 – 4921SubstrateBy similarity
Binding sitei494 – 4941SubstrateBy similarity

GO - Molecular functioni

  • aspartyl esterase activity Source: UniProtKB-KW
  • pectinesterase activity Source: TAIR
  • pectinesterase inhibitor activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT1G53840-MONOMER.
UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectinesterase 1 (EC:3.1.1.11)
Short name:
PE 1
Alternative name(s):
Pectin methylesterase 1
Short name:
AtPME1
Gene namesi
Name:PME1
Synonyms:ARATH65
Ordered Locus Names:At1g53840
ORF Names:T18A20.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G53840.

Subcellular locationi

  • Secretedcell wall 1 Publication
  • Golgi apparatus membrane 1 Publication

  • Note: Cleaved in the Golgi apparatus by SBT6.1 (S1P) after the Arg-Arg-Leu-Met (RRLM) and Arg-Arg-Leu-Leu (RRLL) motifs. This processing is required for extracellular targeting.1 Publication

GO - Cellular componenti

  • cell wall Source: UniProtKB-SubCell
  • endosome Source: TAIR
  • extracellular region Source: TAIR
  • Golgi apparatus Source: TAIR
  • Golgi membrane Source: UniProtKB-SubCell
  • membrane Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • trans-Golgi network Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4949Sequence analysisAdd
BLAST
Chaini50 – 586537Pectinesterase 1PRO_0000023474Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence analysis
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence analysis
Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence analysis
Glycosylationi201 – 2011N-linked (GlcNAc...)Sequence analysis
Glycosylationi207 – 2071N-linked (GlcNAc...)Sequence analysis
Disulfide bondi422 ↔ 442By similarity
Glycosylationi466 – 4661N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ43867.
PRIDEiQ43867.

Expressioni

Tissue specificityi

Expressed in siliques.1 Publication

Developmental stagei

Expressed throughout silique development.1 Publication

Gene expression databases

ExpressionAtlasiQ43867. baseline and differential.
GenevisibleiQ43867. AT.

Interactioni

Subunit structurei

Interacts with SBT6.1.1 Publication

Protein-protein interaction databases

STRINGi3702.AT1G53840.1.

Structurei

3D structure databases

ProteinModelPortaliQ43867.
SMRiQ43867. Positions 280-581.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi250 – 2534RRLM cleavage motif1 Publication
Motifi269 – 2724RRLL cleavage motif1 Publication

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi248 – 2514Poly-Arg

Domaini

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport. The PMEI region is cleaved by SBT6.1 (S1P) in the Golgi apparatus prior to cell wall targeting.1 Publication

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFEH. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiQ43867.
OMAiNSFKGYG.
OrthoDBiEOG093606MM.
PhylomeDBiQ43867.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43867-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSVNSFKGY GKVDEAQDLA LKKKTRKRLL LLSISVVVLI AVIIAAVVAT
60 70 80 90 100
VVHKNKNEST PSPPPELTPS TSLKAICSVT RFPESCISSI SKLPSSNTTD
110 120 130 140 150
PETLFKLSLK VIIDELDSIS DLPEKLSKET EDERIKSALR VCGDLIEDAL
160 170 180 190 200
DRLNDTVSAI DDEEKKKTLS SSKIEDLKTW LSATVTDHET CFDSLDELKQ
210 220 230 240 250
NKTEYANSTI TQNLKSAMSR STEFTSNSLA IVSKILSALS DLGIPIHRRR
260 270 280 290 300
RLMSHHHQQS VDFEKWARRR LLQTAGLKPD VTVAGDGTGD VLTVNEAVAK
310 320 330 340 350
VPKKSLKMFV IYVKSGTYVE NVVMDKSKWN VMIYGDGKGK TIISGSKNFV
360 370 380 390 400
DGTPTYETAT FAIQGKGFIM KDIGIINTAG AAKHQAVAFR SGSDFSVYYQ
410 420 430 440 450
CSFDGFQDTL YPHSNRQFYR DCDVTGTIDF IFGSAAVVFQ GCKIMPRQPL
460 470 480 490 500
SNQFNTITAQ GKKDPNQSSG MSIQRCTISA NGNVIAPTYL GRPWKEFSTT
510 520 530 540 550
VIMETVIGAV VRPSGWMSWV SGVDPPASIV YGEYKNTGPG SDVTQRVKWA
560 570 580
GYKPVMSDAE AAKFTVATLL HGADWIPATG VINQLS
Length:586
Mass (Da):64,149
Last modified:November 1, 1996 - v1
Checksum:iEF2752BCC535BE99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81585 mRNA. Translation: CAA57275.1.
U25649 Genomic DNA. Translation: AAC50024.1.
AC009324 Genomic DNA. Translation: AAF02857.1.
CP002684 Genomic DNA. Translation: AEE33008.1.
AY054197 mRNA. Translation: AAL06858.1.
PIRiJC4778.
RefSeqiNP_175787.1. NM_104261.2.
UniGeneiAt.24997.

Genome annotation databases

EnsemblPlantsiAT1G53840.1; AT1G53840.1; AT1G53840.
GeneIDi841821.
GrameneiAT1G53840.1; AT1G53840.1; AT1G53840.
KEGGiath:AT1G53840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81585 mRNA. Translation: CAA57275.1.
U25649 Genomic DNA. Translation: AAC50024.1.
AC009324 Genomic DNA. Translation: AAF02857.1.
CP002684 Genomic DNA. Translation: AEE33008.1.
AY054197 mRNA. Translation: AAL06858.1.
PIRiJC4778.
RefSeqiNP_175787.1. NM_104261.2.
UniGeneiAt.24997.

3D structure databases

ProteinModelPortaliQ43867.
SMRiQ43867. Positions 280-581.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G53840.1.

Proteomic databases

PaxDbiQ43867.
PRIDEiQ43867.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G53840.1; AT1G53840.1; AT1G53840.
GeneIDi841821.
GrameneiAT1G53840.1; AT1G53840.1; AT1G53840.
KEGGiath:AT1G53840.

Organism-specific databases

TAIRiAT1G53840.

Phylogenomic databases

eggNOGiENOG410IFEH. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiQ43867.
OMAiNSFKGYG.
OrthoDBiEOG093606MM.
PhylomeDBiQ43867.

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.
BioCyciARA:AT1G53840-MONOMER.

Miscellaneous databases

PROiQ43867.

Gene expression databases

ExpressionAtlasiQ43867. baseline and differential.
GenevisibleiQ43867. AT.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPME1_ARATH
AccessioniPrimary (citable) accession number: Q43867
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.