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Protein

Beta-fructofuranosidase, insoluble isoenzyme CWINV1

Gene

CWINV1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-fructofuranosidase that can use sucrose and 1-kestose, and, to a lower extent, neokestose and levan, as substrates, but not inuline.2 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.PROSITE-ProRule annotation

Kineticsi

  1. KM=0.35 mM for sucrose (at pH 5 and 30 degrees Celsius)1 Publication
  2. KM=1 mM for 1-kestose (at pH 5 and 30 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei661
    Binding sitei82Substrate1
    Binding sitei90Substrate1
    Binding sitei246Substrate1
    Binding sitei282Substrate1

    GO - Molecular functioni

    • beta-fructofuranosidase activity Source: TAIR
    • sucrose alpha-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    • carbohydrate metabolic process Source: InterPro
    • response to karrikin Source: TAIR
    • response to wounding Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT3G13790-MONOMER.
    BRENDAi3.2.1.26. 399.

    Protein family/group databases

    CAZyiGH32. Glycoside Hydrolase Family 32.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-fructofuranosidase, insoluble isoenzyme CWINV1 (EC:3.2.1.26)
    Alternative name(s):
    Cell wall beta-fructosidase 1
    Short name:
    AtbetaFRUCT1
    Cell wall invertase 1
    Short name:
    AtcwINV1
    Sucrose hydrolase 1
    Gene namesi
    Name:CWINV1
    Synonyms:BFRUCT1
    Ordered Locus Names:At3g13790
    ORF Names:MMM17.26
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    TAIRiAT3G13790.

    Subcellular locationi

    GO - Cellular componenti

    • apoplast Source: UniProtKB-SubCell
    • cell wall Source: TAIR
    • plasma membrane Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi63W → L: Loss of activity. 1 Publication1
    Mutagenesisi66D → A: Loss of activity. 2 Publications1
    Mutagenesisi90W → L: Loss of activity. 1 Publication1
    Mutagenesisi125W → L: Loss of activity. 1 Publication1
    Mutagenesisi246E → A or Q: Loss of activity. 3 Publications1
    Mutagenesisi282D → A: Impaired beta-fructofuranosidase activity but enhanced 1-fructan exohydrolase activity. 2 Publications1
    Mutagenesisi282D → F: Loss of activity. 2 Publications1
    Mutagenesisi282D → N: Normal activity. 2 Publications1
    Mutagenesisi285K → L: Slightly reduced activity. 1 Publication1
    Mutagenesisi342N → D: Reduced activity and glycosylation. 2 Publications1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 28Sequence analysisAdd BLAST28
    ChainiPRO_000034834729 – 584Beta-fructofuranosidase, insoluble isoenzyme CWINV1Add BLAST556

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi159N-linked (GlcNAc...)1
    Glycosylationi186N-linked (GlcNAc...)1
    Glycosylationi342N-linked (GlcNAc...)1
    Disulfide bondi442 ↔ 491
    Glycosylationi446N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ43866.
    PRIDEiQ43866.

    Expressioni

    Tissue specificityi

    Expressed in seedlings, leaves, flowers, and seeds.3 Publications

    Inductioni

    By wounding, aeroponic growth condition, darkness, sucrose, glucose and mannitol.2 Publications

    Gene expression databases

    ExpressionAtlasiQ43866. baseline and differential.
    GenevisibleiQ43866. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi5925. 1 interactor.
    DIPiDIP-59391N.
    STRINGi3702.AT3G13790.1.

    Structurei

    Secondary structure

    1584
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi50 – 52Combined sources3
    Beta strandi54 – 56Combined sources3
    Beta strandi60 – 73Combined sources14
    Beta strandi76 – 83Combined sources8
    Beta strandi88 – 90Combined sources3
    Beta strandi94 – 113Combined sources20
    Helixi118 – 120Combined sources3
    Beta strandi124 – 131Combined sources8
    Beta strandi137 – 144Combined sources8
    Beta strandi150 – 158Combined sources9
    Beta strandi168 – 170Combined sources3
    Turni181 – 183Combined sources3
    Beta strandi189 – 191Combined sources3
    Beta strandi200 – 202Combined sources3
    Beta strandi204 – 212Combined sources9
    Beta strandi215 – 228Combined sources14
    Beta strandi236 – 240Combined sources5
    Beta strandi245 – 256Combined sources12
    Beta strandi258 – 260Combined sources3
    Beta strandi269 – 271Combined sources3
    Beta strandi273 – 281Combined sources9
    Turni282 – 284Combined sources3
    Beta strandi287 – 295Combined sources9
    Turni296 – 299Combined sources4
    Beta strandi300 – 303Combined sources4
    Beta strandi309 – 312Combined sources4
    Beta strandi318 – 320Combined sources3
    Beta strandi322 – 329Combined sources8
    Turni330 – 333Combined sources4
    Beta strandi334 – 341Combined sources8
    Helixi347 – 353Combined sources7
    Beta strandi354 – 356Combined sources3
    Beta strandi363 – 367Combined sources5
    Beta strandi371 – 378Combined sources8
    Helixi380 – 385Combined sources6
    Beta strandi391 – 398Combined sources8
    Beta strandi402 – 405Combined sources4
    Beta strandi413 – 421Combined sources9
    Helixi425 – 427Combined sources3
    Beta strandi428 – 430Combined sources3
    Helixi438 – 444Combined sources7
    Beta strandi452 – 462Combined sources11
    Beta strandi466 – 468Combined sources3
    Beta strandi470 – 480Combined sources11
    Beta strandi486 – 493Combined sources8
    Beta strandi501 – 503Combined sources3
    Beta strandi508 – 512Combined sources5
    Beta strandi520 – 527Combined sources8
    Beta strandi530 – 535Combined sources6
    Turni536 – 539Combined sources4
    Beta strandi540 – 545Combined sources6
    Beta strandi549 – 551Combined sources3
    Helixi553 – 555Combined sources3
    Beta strandi557 – 562Combined sources6
    Beta strandi564 – 566Combined sources3
    Beta strandi568 – 578Combined sources11

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2AC1X-ray2.15A44-584[»]
    2OXBX-ray2.60A48-584[»]
    2QQUX-ray2.84A48-582[»]
    2QQVX-ray3.01A48-584[»]
    2QQWX-ray2.80A48-584[»]
    2XQRX-ray2.58A/C/E/G/I/K48-584[»]
    ProteinModelPortaliQ43866.
    SMRiQ43866.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ43866.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni63 – 66Substrate binding4
    Regioni125 – 126Substrate binding2
    Regioni191 – 192Substrate binding2

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 32 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG0228. Eukaryota.
    COG1621. LUCA.
    HOGENOMiHOG000181427.
    InParanoidiQ43866.
    KOiK01193.
    OMAiKHDYYTI.
    OrthoDBiEOG093604TK.
    PhylomeDBiQ43866.

    Family and domain databases

    Gene3Di2.115.10.20. 1 hit.
    2.60.120.560. 2 hits.
    InterProiIPR013320. ConA-like_dom.
    IPR001362. Glyco_hydro_32.
    IPR018053. Glyco_hydro_32_AS.
    IPR013189. Glyco_hydro_32_C.
    IPR013148. Glyco_hydro_32_N.
    IPR023296. Glyco_hydro_beta-prop.
    [Graphical view]
    PfamiPF08244. Glyco_hydro_32C. 1 hit.
    PF00251. Glyco_hydro_32N. 1 hit.
    [Graphical view]
    SMARTiSM00640. Glyco_32. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 2 hits.
    SSF75005. SSF75005. 1 hit.
    PROSITEiPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: Q43866-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MTKEVCSNIG LWLLLTLLIG NYVVNLEASH HVYKRLTQST NTKSPSVNQP
    60 70 80 90 100
    YRTGFHFQPP KNWMNDPNGP MIYKGIYHLF YQWNPKGAVW GNIVWAHSTS
    110 120 130 140 150
    TDLINWDPHP PAIFPSAPFD INGCWSGSAT ILPNGKPVIL YTGIDPKNQQ
    160 170 180 190 200
    VQNIAEPKNL SDPYLREWKK SPLNPLMAPD AVNGINASSF RDPTTAWLGQ
    210 220 230 240 250
    DKKWRVIIGS KIHRRGLAIT YTSKDFLKWE KSPEPLHYDD GSGMWECPDF
    260 270 280 290 300
    FPVTRFGSNG VETSSFGEPN EILKHVLKIS LDDTKHDYYT IGTYDRVKDK
    310 320 330 340 350
    FVPDNGFKMD GTAPRYDYGK YYASKTFFDS AKNRRILWGW TNESSSVEDD
    360 370 380 390 400
    VEKGWSGIQT IPRKIWLDRS GKQLIQWPVR EVERLRTKQV KNLRNKVLKS
    410 420 430 440 450
    GSRLEVYGVT AAQADVEVLF KVRDLEKADV IEPSWTDPQL ICSKMNVSVK
    460 470 480 490 500
    SGLGPFGLMV LASKNLEEYT SVYFRIFKAR QNSNKYVVLM CSDQSRSSLK
    510 520 530 540 550
    EDNDKTTYGA FVDINPHQPL SLRALIDHSV VESFGGKGRA CITSRVYPKL
    560 570 580
    AIGKSSHLFA FNYGYQSVDV LNLNAWSMNS AQIS
    Length:584
    Mass (Da):66,280
    Last modified:November 1, 1996 - v1
    Checksum:iC571359FB1825E36
    GO

    Sequence cautioni

    The sequence BAB01930 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X74514 mRNA. Translation: CAA52619.1.
    X74515 mRNA. Translation: CAA52620.1.
    AP001307 Genomic DNA. Translation: BAB01930.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75414.1.
    AY045776 mRNA. Translation: AAK76450.1.
    AY079422 mRNA. Translation: AAL85153.1.
    PIRiS37212.
    RefSeqiNP_001189881.1. NM_001202952.1.
    NP_566464.1. NM_112232.4. [Q43866-1]
    UniGeneiAt.24308.

    Genome annotation databases

    EnsemblPlantsiAT3G13790.1; AT3G13790.1; AT3G13790. [Q43866-1]
    GeneIDi820591.
    GrameneiAT3G13790.1; AT3G13790.1; AT3G13790.
    KEGGiath:AT3G13790.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X74514 mRNA. Translation: CAA52619.1.
    X74515 mRNA. Translation: CAA52620.1.
    AP001307 Genomic DNA. Translation: BAB01930.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75414.1.
    AY045776 mRNA. Translation: AAK76450.1.
    AY079422 mRNA. Translation: AAL85153.1.
    PIRiS37212.
    RefSeqiNP_001189881.1. NM_001202952.1.
    NP_566464.1. NM_112232.4. [Q43866-1]
    UniGeneiAt.24308.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2AC1X-ray2.15A44-584[»]
    2OXBX-ray2.60A48-584[»]
    2QQUX-ray2.84A48-582[»]
    2QQVX-ray3.01A48-584[»]
    2QQWX-ray2.80A48-584[»]
    2XQRX-ray2.58A/C/E/G/I/K48-584[»]
    ProteinModelPortaliQ43866.
    SMRiQ43866.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi5925. 1 interactor.
    DIPiDIP-59391N.
    STRINGi3702.AT3G13790.1.

    Protein family/group databases

    CAZyiGH32. Glycoside Hydrolase Family 32.

    Proteomic databases

    PaxDbiQ43866.
    PRIDEiQ43866.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT3G13790.1; AT3G13790.1; AT3G13790. [Q43866-1]
    GeneIDi820591.
    GrameneiAT3G13790.1; AT3G13790.1; AT3G13790.
    KEGGiath:AT3G13790.

    Organism-specific databases

    TAIRiAT3G13790.

    Phylogenomic databases

    eggNOGiKOG0228. Eukaryota.
    COG1621. LUCA.
    HOGENOMiHOG000181427.
    InParanoidiQ43866.
    KOiK01193.
    OMAiKHDYYTI.
    OrthoDBiEOG093604TK.
    PhylomeDBiQ43866.

    Enzyme and pathway databases

    BioCyciARA:AT3G13790-MONOMER.
    BRENDAi3.2.1.26. 399.

    Miscellaneous databases

    EvolutionaryTraceiQ43866.
    PROiQ43866.

    Gene expression databases

    ExpressionAtlasiQ43866. baseline and differential.
    GenevisibleiQ43866. AT.

    Family and domain databases

    Gene3Di2.115.10.20. 1 hit.
    2.60.120.560. 2 hits.
    InterProiIPR013320. ConA-like_dom.
    IPR001362. Glyco_hydro_32.
    IPR018053. Glyco_hydro_32_AS.
    IPR013189. Glyco_hydro_32_C.
    IPR013148. Glyco_hydro_32_N.
    IPR023296. Glyco_hydro_beta-prop.
    [Graphical view]
    PfamiPF08244. Glyco_hydro_32C. 1 hit.
    PF00251. Glyco_hydro_32N. 1 hit.
    [Graphical view]
    SMARTiSM00640. Glyco_32. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 2 hits.
    SSF75005. SSF75005. 1 hit.
    PROSITEiPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiINV1_ARATH
    AccessioniPrimary (citable) accession number: Q43866
    Secondary accession number(s): Q9LIB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: November 1, 1996
    Last modified: November 30, 2016
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.