Beta-fructofuranosidase, insoluble isoenzyme CWINV1 precursor - Arabidopsis thaliana (Mouse-ear cress)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Beta-fructofuranosidase, insoluble isoenzyme CWINV1
EC=3.2.1.26

Alternative name(s):
Cell wall beta-fructosidase 1
Short name=AtbetaFRUCT1
Cell wall invertase 1
Short name=AtcwINV1
Sucrose hydrolase 1

Gene names

Name:	CWINV1
Synonyms:	BFRUCT1
Ordered Locus Names:	At3g13790
ORF Names:	MMM17.26

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Beta-fructofuranosidase that can use sucrose and 1-kestose, and, to a lower extent, neokestose and levan, as substrates, but not inuline. Ref.7 Ref.9
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › extracellular space › apoplast Probable. Secreted › cell wall Probable. Note: Associated to the cell wall Probable.
Tissue specificity	Expressed in seedlings, leaves, flowers, and seeds. Ref.5 Ref.6 Ref.8
Induction	By wounding, aeroponic growth condition, darkness, sucrose, glucose and mannitol. Ref.5 Ref.8
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.
Biophysicochemical properties	Kinetic parameters: K_M=0.35 mM for sucrose (at pH 5 and 30 degrees Celsius) Ref.9 K_M=1 mM for 1-kestose (at pH 5 and 30 degrees Celsius)
Sequence caution	The sequence BAB01930.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Apoplast Cell wall Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to karrikin Inferred from expression pattern PubMed 20351290. Source: TAIR response to wounding Inferred from mutant phenotype Ref.8. Source: TAIR sucrose biosynthetic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from direct assay PubMed 15593128. Source: TAIR plasma membrane Inferred from direct assay PubMed 22923678. Source: TAIR
Molecular_function	beta-fructofuranosidase activity Inferred from mutant phenotype Ref.8. Source: TAIR sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q43866-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

28

Potential

Chain

29 – 584

556

Beta-fructofuranosidase, insoluble isoenzyme CWINV1

PRO_0000348347

Regions

Region

63 – 66

4

Substrate binding

Region

125 – 126

2

Substrate binding

Region

191 – 192

2

Substrate binding

Sites

Active site

66

1

Binding site

82

1

Substrate

Binding site

90

1

Substrate

Binding site

246

1

Substrate

Binding site

282

1

Substrate

Amino acid modifications

Glycosylation

159

1

N-linked (GlcNAc...)

Glycosylation

186

1

N-linked (GlcNAc...)

Glycosylation

342

1

N-linked (GlcNAc...)

Glycosylation

446

1

N-linked (GlcNAc...) Potential

Disulfide bond

442 ↔ 491

Experimental info

Mutagenesis

63

1

W → L: Loss of activity. Ref.9

Mutagenesis

66

1

D → A: Loss of activity. Ref.9 Ref.12

Mutagenesis

90

1

W → L: Loss of activity. Ref.9

Mutagenesis

125

1

W → L: Loss of activity. Ref.9

Mutagenesis

246

1

E → A or Q: Loss of activity. Ref.9 Ref.12 Ref.13

Mutagenesis

282

1

D → A: Impaired beta-fructofuranosidase activity but enhanced 1-fructan exohydrolase activity. Ref.9 Ref.12

Mutagenesis

282

1

D → F: Loss of activity. Ref.9 Ref.12

Mutagenesis

282

1

D → N: Normal activity. Ref.9 Ref.12

Mutagenesis

285

1

K → L: Slightly reduced activity. Ref.9

Mutagenesis

342

1

N → D: Reduced activity and glycosylation. Ref.10 Ref.11

Secondary structure

1

.

584

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C571359FB1825E36
Show »

FASTA

584

66,280

        10         20         30         40         50         60 
MTKEVCSNIG LWLLLTLLIG NYVVNLEASH HVYKRLTQST NTKSPSVNQP YRTGFHFQPP 

        70         80         90        100        110        120 
KNWMNDPNGP MIYKGIYHLF YQWNPKGAVW GNIVWAHSTS TDLINWDPHP PAIFPSAPFD 

       130        140        150        160        170        180 
INGCWSGSAT ILPNGKPVIL YTGIDPKNQQ VQNIAEPKNL SDPYLREWKK SPLNPLMAPD 

       190        200        210        220        230        240 
AVNGINASSF RDPTTAWLGQ DKKWRVIIGS KIHRRGLAIT YTSKDFLKWE KSPEPLHYDD 

       250        260        270        280        290        300 
GSGMWECPDF FPVTRFGSNG VETSSFGEPN EILKHVLKIS LDDTKHDYYT IGTYDRVKDK 

       310        320        330        340        350        360 
FVPDNGFKMD GTAPRYDYGK YYASKTFFDS AKNRRILWGW TNESSSVEDD VEKGWSGIQT 

       370        380        390        400        410        420 
IPRKIWLDRS GKQLIQWPVR EVERLRTKQV KNLRNKVLKS GSRLEVYGVT AAQADVEVLF 

       430        440        450        460        470        480 
KVRDLEKADV IEPSWTDPQL ICSKMNVSVK SGLGPFGLMV LASKNLEEYT SVYFRIFKAR 

       490        500        510        520        530        540 
QNSNKYVVLM CSDQSRSSLK EDNDKTTYGA FVDINPHQPL SLRALIDHSV VESFGGKGRA 

       550        560        570        580 
CITSRVYPKL AIGKSSHLFA FNYGYQSVDV LNLNAWSMNS AQIS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis gene and cDNA encoding cell-wall invertase." Schwebel-Dugue N., el Mtili N., Krivitzky M., Jean-Jacques I., Williams J.H., Thomas M., Kreis M., Lecharny A. Plant Physiol. 104:809-810(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Expression of the Arabidopsis thaliana invertase gene family." Tymowska-Lalanne Z., Kreis M. Planta 207:259-265(1998) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[6]	"Roles of cell-wall invertases and monosaccharide transporters in the growth and development of Arabidopsis." Sherson S.M., Alford H.L., Forbes S.M., Wallace G., Smith S.M. J. Exp. Bot. 54:525-531(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[7]	"Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities." de Coninck B., Le Roy K., Francis I., Clerens S., Vergauwen R., Halliday A.M., Smith S.M., Van Laere A., Van Den Ende W. Plant Cell Environ. 28:432-443(2005) Cited for: FUNCTION.
[8]	"Imaging photosynthesis in wounded leaves of Arabidopsis thaliana." Quilliam R.S., Swarbrick P.J., Scholes J.D., Rolfe S.A. J. Exp. Bot. 57:55-69(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION BY WOUNDING.
[9]	"Unraveling the difference between invertases and fructan exohydrolases: a single amino acid (Asp-239) substitution transforms Arabidopsis cell wall invertase1 into a fructan 1-exohydrolase." Le Roy K., Lammens W., Verhaest M., De Coninck B., Rabijns A., Van Laere A., Van den Ende W. Plant Physiol. 145:616-625(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF TRP-63; ASP-66; TRP-90; TRP-125; GLU-246; ASP-282 AND LYS-285, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE BINDING.
[10]	"N-glycosylation affects substrate specificity of chicory fructan 1-exohydrolase: evidence for the presence of an inulin binding cleft." Le Roy K., Verhaest M., Rabijns A., Clerens S., Van Laere A., Van den Ende W. New Phytol. 176:317-324(2007) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF ASN-342.
[11]	"X-ray diffraction structure of a cell-wall invertase from Arabidopsis thaliana." Verhaest M., Lammens W., Le Roy K., De Coninck B., De Ranter C.J., Van Laere A., Van den Ende W., Rabijns A. Acta Crystallogr. D 62:1555-1563(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 44-584 IN COMPLEX WITH SUBSTRATES, MUTAGENESIS OF ASN-342, GLYCOSYLATION.
[12]	"Crystal structures of Arabidopsis thaliana cell-wall invertase mutants in complex with sucrose." Lammens W., Le Roy K., Van Laere A., Rabijns A., Van den Ende W. J. Mol. Biol. 377:378-385(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 48-584 IN COMPLEX WITH SUBSTRATES, MUTAGENESIS OF ASP-66; GLU-246 AND ASP-282, GLYCOSYLATION.
[13]	"An alternate sucrose binding mode in the E203Q Arabidopsis invertase mutant: an X-ray crystallography and docking study." Matrai J., Lammens W., Jonckheer A., Le Roy K., Rabijns A., Van den Ende W., De Maeyer M. Proteins 71:552-564(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 48-584 IN COMPLEX WITH SUBSTRATES, MUTAGENESIS OF GLU-246, GLYCOSYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X74514 mRNA. Translation: CAA52619.1.
X74515 mRNA. Translation: CAA52620.1.
AP001307 Genomic DNA. Translation: BAB01930.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75414.1.
AY045776 mRNA. Translation: AAK76450.1.
AY079422 mRNA. Translation: AAL85153.1.

IPI

IPI00518427.

PIR

S37212.

RefSeq

NP_001189881.1. NM_001202952.1.
NP_566464.1. NM_112232.3.

UniGene

At.24308.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2AC1	X-ray	2.15	A	44-584	[»]
2OXB	X-ray	2.60	A	48-584	[»]
2QQU	X-ray	2.84	A	48-582	[»]
2QQV	X-ray	3.01	A	48-584	[»]
2QQW	X-ray	2.80	A	48-584	[»]
2XQR	X-ray	2.58	A/C/E/G/I/K	48-584	[»]

ProteinModelPortal

Q43866.

SMR

Q43866. Positions 48-584.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59391N.

STRING

3702.AT3G13790.1-P.

Protein family/group databases

CAZy

GH32. Glycoside Hydrolase Family 32.

Proteomic databases

PaxDb

Q43866.

PRIDE

Q43866.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT3G13790.1; AT3G13790.1; AT3G13790.

GeneID

820591.

KEGG

ath:AT3G13790.

Organism-specific databases

TAIR

At3g13790.

Phylogenomic databases

eggNOG

COG1621.

HOGENOM

HOG000181427.

InParanoid

Q43866.

KO

K01193.

OMA

CIANPPK.

PhylomeDB

Q43866.

ProtClustDB

CLSN2914992.

Gene expression databases

ArrayExpress

Q43866.

Genevestigator

Q43866.

Family and domain databases

Gene3D

2.115.10.20. 1 hit.

InterPro

IPR008985. ConA-like_lec_gl_sf.
IPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_beta-prop.
[Graphical view]

Pfam

PF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]

SMART

SM00640. Glyco_32. 1 hit.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.
SSF75005. Glyco_hydro_43_beta-prop. 1 hit.

PROSITE

PS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q43866.

Entry information

Entry name

INV1_ARATH

Accession

Primary (citable) accession number: Q43866
Secondary accession number(s): Q9LIB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families