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Protein

Beta-fructofuranosidase, insoluble isoenzyme CWINV1

Gene

CWINV1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-fructofuranosidase that can use sucrose and 1-kestose, and, to a lower extent, neokestose and levan, as substrates, but not inuline.2 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.PROSITE-ProRule annotation

Kineticsi

  1. KM=0.35 mM for sucrose (at pH 5 and 30 degrees Celsius)1 Publication
  2. KM=1 mM for 1-kestose (at pH 5 and 30 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei66 – 661
    Binding sitei82 – 821Substrate
    Binding sitei90 – 901Substrate
    Binding sitei246 – 2461Substrate
    Binding sitei282 – 2821Substrate

    GO - Molecular functioni

    • beta-fructofuranosidase activity Source: TAIR
    • sucrose alpha-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    • carbohydrate metabolic process Source: InterPro
    • response to karrikin Source: TAIR
    • response to wounding Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT3G13790-MONOMER.
    ARA:GQT-2630-MONOMER.
    BRENDAi3.2.1.26. 399.

    Protein family/group databases

    CAZyiGH32. Glycoside Hydrolase Family 32.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-fructofuranosidase, insoluble isoenzyme CWINV1 (EC:3.2.1.26)
    Alternative name(s):
    Cell wall beta-fructosidase 1
    Short name:
    AtbetaFRUCT1
    Cell wall invertase 1
    Short name:
    AtcwINV1
    Sucrose hydrolase 1
    Gene namesi
    Name:CWINV1
    Synonyms:BFRUCT1
    Ordered Locus Names:At3g13790
    ORF Names:MMM17.26
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    TAIRiAT3G13790.

    Subcellular locationi

    GO - Cellular componenti

    • apoplast Source: UniProtKB-SubCell
    • cell wall Source: TAIR
    • plasma membrane Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi63 – 631W → L: Loss of activity. 1 Publication
    Mutagenesisi66 – 661D → A: Loss of activity. 2 Publications
    Mutagenesisi90 – 901W → L: Loss of activity. 1 Publication
    Mutagenesisi125 – 1251W → L: Loss of activity. 1 Publication
    Mutagenesisi246 – 2461E → A or Q: Loss of activity. 3 Publications
    Mutagenesisi282 – 2821D → A: Impaired beta-fructofuranosidase activity but enhanced 1-fructan exohydrolase activity. 2 Publications
    Mutagenesisi282 – 2821D → F: Loss of activity. 2 Publications
    Mutagenesisi282 – 2821D → N: Normal activity. 2 Publications
    Mutagenesisi285 – 2851K → L: Slightly reduced activity. 1 Publication
    Mutagenesisi342 – 3421N → D: Reduced activity and glycosylation. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence analysisAdd
    BLAST
    Chaini29 – 584556Beta-fructofuranosidase, insoluble isoenzyme CWINV1PRO_0000348347Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi159 – 1591N-linked (GlcNAc...)
    Glycosylationi186 – 1861N-linked (GlcNAc...)
    Glycosylationi342 – 3421N-linked (GlcNAc...)
    Disulfide bondi442 ↔ 491
    Glycosylationi446 – 4461N-linked (GlcNAc...)Sequence analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ43866.
    PRIDEiQ43866.

    Expressioni

    Tissue specificityi

    Expressed in seedlings, leaves, flowers, and seeds.3 Publications

    Inductioni

    By wounding, aeroponic growth condition, darkness, sucrose, glucose and mannitol.2 Publications

    Gene expression databases

    ExpressionAtlasiQ43866. baseline and differential.
    GenevisibleiQ43866. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi5925. 1 interaction.
    DIPiDIP-59391N.
    STRINGi3702.AT3G13790.1.

    Structurei

    Secondary structure

    1
    584
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi50 – 523Combined sources
    Beta strandi54 – 563Combined sources
    Beta strandi60 – 7314Combined sources
    Beta strandi76 – 838Combined sources
    Beta strandi88 – 903Combined sources
    Beta strandi94 – 11320Combined sources
    Helixi118 – 1203Combined sources
    Beta strandi124 – 1318Combined sources
    Beta strandi137 – 1448Combined sources
    Beta strandi150 – 1589Combined sources
    Beta strandi168 – 1703Combined sources
    Turni181 – 1833Combined sources
    Beta strandi189 – 1913Combined sources
    Beta strandi200 – 2023Combined sources
    Beta strandi204 – 2129Combined sources
    Beta strandi215 – 22814Combined sources
    Beta strandi236 – 2405Combined sources
    Beta strandi245 – 25612Combined sources
    Beta strandi258 – 2603Combined sources
    Beta strandi269 – 2713Combined sources
    Beta strandi273 – 2819Combined sources
    Turni282 – 2843Combined sources
    Beta strandi287 – 2959Combined sources
    Turni296 – 2994Combined sources
    Beta strandi300 – 3034Combined sources
    Beta strandi309 – 3124Combined sources
    Beta strandi318 – 3203Combined sources
    Beta strandi322 – 3298Combined sources
    Turni330 – 3334Combined sources
    Beta strandi334 – 3418Combined sources
    Helixi347 – 3537Combined sources
    Beta strandi354 – 3563Combined sources
    Beta strandi363 – 3675Combined sources
    Beta strandi371 – 3788Combined sources
    Helixi380 – 3856Combined sources
    Beta strandi391 – 3988Combined sources
    Beta strandi402 – 4054Combined sources
    Beta strandi413 – 4219Combined sources
    Helixi425 – 4273Combined sources
    Beta strandi428 – 4303Combined sources
    Helixi438 – 4447Combined sources
    Beta strandi452 – 46211Combined sources
    Beta strandi466 – 4683Combined sources
    Beta strandi470 – 48011Combined sources
    Beta strandi486 – 4938Combined sources
    Beta strandi501 – 5033Combined sources
    Beta strandi508 – 5125Combined sources
    Beta strandi520 – 5278Combined sources
    Beta strandi530 – 5356Combined sources
    Turni536 – 5394Combined sources
    Beta strandi540 – 5456Combined sources
    Beta strandi549 – 5513Combined sources
    Helixi553 – 5553Combined sources
    Beta strandi557 – 5626Combined sources
    Beta strandi564 – 5663Combined sources
    Beta strandi568 – 57811Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AC1X-ray2.15A44-584[»]
    2OXBX-ray2.60A48-584[»]
    2QQUX-ray2.84A48-582[»]
    2QQVX-ray3.01A48-584[»]
    2QQWX-ray2.80A48-584[»]
    2XQRX-ray2.58A/C/E/G/I/K48-584[»]
    ProteinModelPortaliQ43866.
    SMRiQ43866. Positions 48-584.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ43866.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni63 – 664Substrate binding
    Regioni125 – 1262Substrate binding
    Regioni191 – 1922Substrate binding

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 32 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG0228. Eukaryota.
    COG1621. LUCA.
    HOGENOMiHOG000181427.
    InParanoidiQ43866.
    KOiK01193.
    OMAiKHDYYTI.
    OrthoDBiEOG093604TK.
    PhylomeDBiQ43866.

    Family and domain databases

    Gene3Di2.115.10.20. 1 hit.
    2.60.120.560. 2 hits.
    InterProiIPR013320. ConA-like_dom.
    IPR001362. Glyco_hydro_32.
    IPR018053. Glyco_hydro_32_AS.
    IPR013189. Glyco_hydro_32_C.
    IPR013148. Glyco_hydro_32_N.
    IPR023296. Glyco_hydro_beta-prop.
    [Graphical view]
    PfamiPF08244. Glyco_hydro_32C. 1 hit.
    PF00251. Glyco_hydro_32N. 1 hit.
    [Graphical view]
    SMARTiSM00640. Glyco_32. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 2 hits.
    SSF75005. SSF75005. 1 hit.
    PROSITEiPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: Q43866-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MTKEVCSNIG LWLLLTLLIG NYVVNLEASH HVYKRLTQST NTKSPSVNQP
    60 70 80 90 100
    YRTGFHFQPP KNWMNDPNGP MIYKGIYHLF YQWNPKGAVW GNIVWAHSTS
    110 120 130 140 150
    TDLINWDPHP PAIFPSAPFD INGCWSGSAT ILPNGKPVIL YTGIDPKNQQ
    160 170 180 190 200
    VQNIAEPKNL SDPYLREWKK SPLNPLMAPD AVNGINASSF RDPTTAWLGQ
    210 220 230 240 250
    DKKWRVIIGS KIHRRGLAIT YTSKDFLKWE KSPEPLHYDD GSGMWECPDF
    260 270 280 290 300
    FPVTRFGSNG VETSSFGEPN EILKHVLKIS LDDTKHDYYT IGTYDRVKDK
    310 320 330 340 350
    FVPDNGFKMD GTAPRYDYGK YYASKTFFDS AKNRRILWGW TNESSSVEDD
    360 370 380 390 400
    VEKGWSGIQT IPRKIWLDRS GKQLIQWPVR EVERLRTKQV KNLRNKVLKS
    410 420 430 440 450
    GSRLEVYGVT AAQADVEVLF KVRDLEKADV IEPSWTDPQL ICSKMNVSVK
    460 470 480 490 500
    SGLGPFGLMV LASKNLEEYT SVYFRIFKAR QNSNKYVVLM CSDQSRSSLK
    510 520 530 540 550
    EDNDKTTYGA FVDINPHQPL SLRALIDHSV VESFGGKGRA CITSRVYPKL
    560 570 580
    AIGKSSHLFA FNYGYQSVDV LNLNAWSMNS AQIS
    Length:584
    Mass (Da):66,280
    Last modified:November 1, 1996 - v1
    Checksum:iC571359FB1825E36
    GO

    Sequence cautioni

    The sequence BAB01930 differs from that shown. Reason: Erroneous gene model prediction. Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X74514 mRNA. Translation: CAA52619.1.
    X74515 mRNA. Translation: CAA52620.1.
    AP001307 Genomic DNA. Translation: BAB01930.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75414.1.
    AY045776 mRNA. Translation: AAK76450.1.
    AY079422 mRNA. Translation: AAL85153.1.
    PIRiS37212.
    RefSeqiNP_001189881.1. NM_001202952.1.
    NP_566464.1. NM_112232.3. [Q43866-1]
    UniGeneiAt.24308.

    Genome annotation databases

    EnsemblPlantsiAT3G13790.1; AT3G13790.1; AT3G13790. [Q43866-1]
    GeneIDi820591.
    KEGGiath:AT3G13790.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X74514 mRNA. Translation: CAA52619.1.
    X74515 mRNA. Translation: CAA52620.1.
    AP001307 Genomic DNA. Translation: BAB01930.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75414.1.
    AY045776 mRNA. Translation: AAK76450.1.
    AY079422 mRNA. Translation: AAL85153.1.
    PIRiS37212.
    RefSeqiNP_001189881.1. NM_001202952.1.
    NP_566464.1. NM_112232.3. [Q43866-1]
    UniGeneiAt.24308.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AC1X-ray2.15A44-584[»]
    2OXBX-ray2.60A48-584[»]
    2QQUX-ray2.84A48-582[»]
    2QQVX-ray3.01A48-584[»]
    2QQWX-ray2.80A48-584[»]
    2XQRX-ray2.58A/C/E/G/I/K48-584[»]
    ProteinModelPortaliQ43866.
    SMRiQ43866. Positions 48-584.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi5925. 1 interaction.
    DIPiDIP-59391N.
    STRINGi3702.AT3G13790.1.

    Protein family/group databases

    CAZyiGH32. Glycoside Hydrolase Family 32.

    Proteomic databases

    PaxDbiQ43866.
    PRIDEiQ43866.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT3G13790.1; AT3G13790.1; AT3G13790. [Q43866-1]
    GeneIDi820591.
    KEGGiath:AT3G13790.

    Organism-specific databases

    TAIRiAT3G13790.

    Phylogenomic databases

    eggNOGiKOG0228. Eukaryota.
    COG1621. LUCA.
    HOGENOMiHOG000181427.
    InParanoidiQ43866.
    KOiK01193.
    OMAiKHDYYTI.
    OrthoDBiEOG093604TK.
    PhylomeDBiQ43866.

    Enzyme and pathway databases

    BioCyciARA:AT3G13790-MONOMER.
    ARA:GQT-2630-MONOMER.
    BRENDAi3.2.1.26. 399.

    Miscellaneous databases

    EvolutionaryTraceiQ43866.
    PROiQ43866.

    Gene expression databases

    ExpressionAtlasiQ43866. baseline and differential.
    GenevisibleiQ43866. AT.

    Family and domain databases

    Gene3Di2.115.10.20. 1 hit.
    2.60.120.560. 2 hits.
    InterProiIPR013320. ConA-like_dom.
    IPR001362. Glyco_hydro_32.
    IPR018053. Glyco_hydro_32_AS.
    IPR013189. Glyco_hydro_32_C.
    IPR013148. Glyco_hydro_32_N.
    IPR023296. Glyco_hydro_beta-prop.
    [Graphical view]
    PfamiPF08244. Glyco_hydro_32C. 1 hit.
    PF00251. Glyco_hydro_32N. 1 hit.
    [Graphical view]
    SMARTiSM00640. Glyco_32. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 2 hits.
    SSF75005. SSF75005. 1 hit.
    PROSITEiPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiINV1_ARATH
    AccessioniPrimary (citable) accession number: Q43866
    Secondary accession number(s): Q9LIB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: November 1, 1996
    Last modified: September 7, 2016
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.