Q43857 (INVA_VICFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acid beta-fructofuranosidase
EC=3.2.1.26

Alternative name(s):
Acid invertase
Short name=AI
Acid sucrose hydrolase
Vacuolar invertase

Gene names

Name:

VCINV

Organism

Vicia faba (Broad bean) (Faba vulgaris)

Taxonomic identifier

3906 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Vicia

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subcellular location	Vacuole Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Vacuole
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological_process	sucrose metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 38	38	Potential
	Propeptide	39 – 95	57	By similarity		PRO_0000033391
	Chain	96 – 642	547	Acid beta-fructofuranosidase		PRO_0000033392
Regions
	Region	119 – 122	4	Substrate binding By similarity
	Region	181 – 182	2	Substrate binding By similarity
	Region	245 – 246	2	Substrate binding By similarity
Sites
	Active site	122	1	By similarity
	Binding site	138	1	Substrate By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	300	1	Substrate By similarity
	Binding site	333	1	Substrate By similarity
Amino acid modifications
	Glycosylation	12	1	N-linked (GlcNAc...) Potential
	Glycosylation	100	1	N-linked (GlcNAc...) Potential
	Glycosylation	267	1	N-linked (GlcNAc...) Potential
	Glycosylation	491	1	N-linked (GlcNAc...) Potential
	Glycosylation	615	1	N-linked (GlcNAc...) Potential
	Disulfide bond	490 ↔ 538		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43857 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 099466BFD288D972	FASTA	642	72,029
	10 20 30 40 50 60 MRNDSPYTPL LNASHNNHRR RELLLLFSGL LLLASIIAFS AYIAQPHADA DVSSSSSILS 70 80 90 100 110 120 DEATRPTTLS RGVSSGVSEK SNTFLLSGNL VGEGGSFPWN NTMLSWQRTA FHFQPEKNWM 130 140 150 160 170 180 NDPNGPLYYK GWYHFFYQYN PNGAVWGDIV WGHAVSRDLI HWLHLPLAMV ADQWYDSNGV 190 200 210 220 230 240 WTGSATILPD GQVIMLYTGS TNEFVQVQNL AYPADLNDPL LVDWIKYPSN PVLVPPPGIL 250 260 270 280 290 300 PKDFRDPTTA WLTTEGKWRI TIGSKINKTG VALVYDTVDF KTYERKDMLL NAVPGTGMWE 310 320 330 340 350 360 CVDFFPVSMK SENGLDTSFT GDEVKHVMKV SLDDDRHDYY ALGTYDEKKV KFIADDFEND 370 380 390 400 410 420 VGIGLRYDYG IFYASKTFYD QKKDRRVLWG WIGESDSEYA DVAKGWASVQ SIPRIVKLDK 430 440 450 460 470 480 KTGSNLLQWP VAEVESLRLR SDEFQNLKVK PGAVVSVDIE TATQLDIVAE FEIDKEALEK 490 500 510 520 530 540 TAQSNVEYEC NTSGGASRRG ALGPFGLYVL ADNGLSEYTP VYFYVVKGIN GKLHTSFCSD 550 560 570 580 590 600 QSRSSLANDV HKQIYGSVVP VLEGEKLSLR ILVDHSIVES FAQGGRTCIT SRVYPTRAIY 610 620 630 640 GAARLFLFNN AIETNVTASL KVWQMNSAFI RPYHPDQKRQ TS

References

[1]

"Seed coat-associated invertases of fava bean control both unloading and storage functions: cloning of cDNAs and cell type-specific expression." Weber H., Borisjuk L., Heim U., Buchner P., Wobus U. Plant Cell 7:1835-1846(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Fribo. Tissue: Seed.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49831 mRNA. Translation: CAA89992.1.
PIR	S55521.
3D structure databases
ProteinModelPortal	Q43857.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00238.
Family and domain databases
Gene3D	2.115.10.20. 1 hit.
InterPro	IPR021792. Beta-fructofuranosidase. IPR008985. ConA-like_lec_gl_sf. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
Pfam	PF11837. DUF3357. 1 hit. PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INVA_VICFA

Accession

Primary (citable) accession number: Q43857

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program