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Q43847

- SSY2_SOLTU

UniProt

Q43847 - SSY2_SOLTU

Protein

Granule-bound starch synthase 2, chloroplastic/amyloplastic

Gene

SS2

Organism
Solanum tuberosum (Potato)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 3 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    Accounts for only 10 to 15% of the total soluble starch synthase activity in tubers.1 Publication

    Catalytic activityi

    ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1).

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei290 – 2901ADP-glucoseBy similarity

    GO - Molecular functioni

    1. starch synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. amylopectin biosynthetic process Source: EnsemblPlants/Gramene
    2. starch biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Starch biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00152.

    Protein family/group databases

    CAZyiGT5. Glycosyltransferase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Granule-bound starch synthase 2, chloroplastic/amyloplastic (EC:2.4.1.21)
    Alternative name(s):
    Granule-bound starch synthase II
    Short name:
    GBSS-II
    Short name:
    SS II
    Gene namesi
    Name:SS2
    Synonyms:SSII
    OrganismiSolanum tuberosum (Potato)
    Taxonomic identifieri4113 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
    ProteomesiUP000011115: Unplaced

    Subcellular locationi

    Plastidchloroplast By similarity. Plastidamyloplast By similarity
    Note: Amyloplast or chloroplast, granule-bound and soluble.By similarity

    GO - Cellular componenti

    1. amyloplast Source: UniProtKB-SubCell
    2. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Amyloplast, Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4545Chloroplast1 PublicationAdd
    BLAST
    Chaini46 – 767722Granule-bound starch synthase 2, chloroplastic/amyloplasticPRO_0000011145Add
    BLAST

    Expressioni

    Inductioni

    Constant expression in light and darkness.

    Structurei

    3D structure databases

    ProteinModelPortaliQ43847.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK00703.

    Family and domain databases

    HAMAPiMF_00484. Glycogen_synth.
    InterProiIPR001296. Glyco_trans_1.
    IPR011835. Glycogen/starch_synth.
    IPR013534. Starch_synth_cat_dom.
    [Graphical view]
    PfamiPF08323. Glyco_transf_5. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02095. glgA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q43847-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENSILLHSG NQFHPNLPLL ALRPKKLSLI HGSSREQMWR IKRVKATGEN    50
    SGEAASADES NDALQVTIEK SKKVLAMQQD LLQQIAERRK VVSSIKSSLA 100
    NAKGTYDGGS GSLSDVDIPD VDKDYNVTVP STAATPITDV DKNTPPAISQ 150
    DFVESKREIK RDLADERAPP LSRSSITASS QISSTVSSKR TLNVPPETPK 200
    SSQETLLDVN SRKSLVDVPG KKIQSYMPSL RKESSASHVE QRNENLEGSS 250
    AEANEETEDP VNIDEKPPPL AGTNVMNIIL VASECAPWSK TGGLGDVAGA 300
    LPKALARRGH RVMVVAPRYD NYPEPQDSGV RKIYKVDGQD VEVTYFQAFI 350
    DGVDFVFIDS HMFRHIGNNI YGGNRVDILK RMVLFCKAAI EVPWHVPCGG 400
    VCYGDGNLVF IANDWHTALL PVYLKAYYRD NGIMNYTRSV LVIHNIAHQG 450
    RGPLEDFSYV DLPPHYMDPF KLYDPVGGEH FNIFAAGLKT ADRVVTVSHG 500
    YSWELKTSQG GWGLHQIINE NDWKLQGIVN GIDTKEWNPE LDVHLQSDGY 550
    MNYSLDTLQT GKPQCKAALQ KELGLPVRDD VPLIGFIGRL DPQKGVDLIA 600
    EAVPWMMGQD VQLVMLGTGR RDLEQMLRQF ECQHNDKIRG WVGFSVKTSH 650
    RITAGADILL MPSRFEPCGL NQLYAMKYGT IPVVHAVGGL RDTVQPFDPF 700
    NESGLGWTFS RAEASQLIHA LGNCLLTYRE YKKSWEGIQT RCMTQDLSWD 750
    NAAQNYEEVL IAAKYQW 767
    Length:767
    Mass (Da):85,222
    Last modified:January 4, 2005 - v3
    Checksum:i7C6405995FEC996B
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti51 – 511S → D.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87988 mRNA. Translation: CAA61241.2.
    PIRiT07667.
    RefSeqiNP_001274977.1. NM_001288048.1.
    UniGeneiStu.662.

    Genome annotation databases

    EnsemblPlantsiPGSC0003DMT400003356; PGSC0003DMT400003356; PGSC0003DMG400001328.
    GeneIDi102583115.
    KEGGisot:102583115.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87988 mRNA. Translation: CAA61241.2 .
    PIRi T07667.
    RefSeqi NP_001274977.1. NM_001288048.1.
    UniGenei Stu.662.

    3D structure databases

    ProteinModelPortali Q43847.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT5. Glycosyltransferase Family 5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi PGSC0003DMT400003356 ; PGSC0003DMT400003356 ; PGSC0003DMG400001328 .
    GeneIDi 102583115.
    KEGGi sot:102583115.

    Phylogenomic databases

    KOi K00703.

    Enzyme and pathway databases

    UniPathwayi UPA00152 .

    Family and domain databases

    HAMAPi MF_00484. Glycogen_synth.
    InterProi IPR001296. Glyco_trans_1.
    IPR011835. Glycogen/starch_synth.
    IPR013534. Starch_synth_cat_dom.
    [Graphical view ]
    Pfami PF08323. Glyco_transf_5. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02095. glgA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Biochemical and molecular characterization of a novel starch synthase from potato tubers."
      Edwards A., Marshall J., Sidebottom C., Visser R.G.F., Smith A.M., Martin C.
      Plant J. 8:283-294(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 46-55, VARIANT ASP-51.
      Strain: cv. Desiree.
      Tissue: Tuber.
    2. "Resequencing, expression and purification of His-tagged potato soluble starch synthase II in Escherichia coli."
      Shearer N., Edwards E.A., Martin C., Bornemann S.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Genome sequence and analysis of the tuber crop potato."
      The Potato Genome Sequencing Consortium
      Nature 475:189-195(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. DM1-3 516 R44.
    4. "Cloning and functional analysis of a cDNA encoding a starch synthase from potato (Solanum tuberosum L.) that is predominantly expressed in leaf tissue."
      Kossmann J., Abel G.J.W., Springer F., Lloyd J.R., Willmitzer L.
      Planta 208:503-511(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiSSY2_SOLTU
    AccessioniPrimary (citable) accession number: Q43847
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 77 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    May be the main starch synthase responsible for the incorporation of phosphorylated precursors into growing alpha-1,4-glucans.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3