Q43847 (SSY2_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Granule-bound starch synthase 2, chloroplastic/amyloplastic
EC=2.4.1.21

Alternative name(s):
Granule-bound starch synthase II
Short name=GBSS-II
Short name=SS II

Gene names

Name:	SS2
Synonyms:	SSII

Organism

Solanum tuberosum (Potato) [Reference proteome]

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accounts for only 10 to 15% of the total soluble starch synthase activity in tubers. Ref.3
Catalytic activity	ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1).
Pathway	Glycan biosynthesis; starch biosynthesis.
Subcellular location	Plastid › chloroplast By similarity. Plastid › amyloplast By similarity. Note: Amyloplast or chloroplast, granule-bound and soluble By similarity.
Induction	Constant expression in light and darkness.
Miscellaneous	May be the main starch synthase responsible for the incorporation of phosphorylated precursors into growing alpha-1,4-glucans.
Sequence similarities	Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.

Ontologies

Keywords
Biological process	Starch biosynthesis
Cellular component	Amyloplast Chloroplast Plastid
Coding sequence diversity	Polymorphism
Domain	Transit peptide
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	starch biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	amyloplast Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	starch synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 45	45	Chloroplast Ref.1
	Chain	46 – 767	722	Granule-bound starch synthase 2, chloroplastic/amyloplastic		PRO_0000011145
Sites
	Binding site	290	1	ADP-glucose By similarity
Natural variations
	Natural variant	51	1	S → D. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q43847 [UniParc]. Last modified January 4, 2005. Version 3. Checksum: 7C6405995FEC996B	FASTA	767	85,222
	10 20 30 40 50 60 MENSILLHSG NQFHPNLPLL ALRPKKLSLI HGSSREQMWR IKRVKATGEN SGEAASADES 70 80 90 100 110 120 NDALQVTIEK SKKVLAMQQD LLQQIAERRK VVSSIKSSLA NAKGTYDGGS GSLSDVDIPD 130 140 150 160 170 180 VDKDYNVTVP STAATPITDV DKNTPPAISQ DFVESKREIK RDLADERAPP LSRSSITASS 190 200 210 220 230 240 QISSTVSSKR TLNVPPETPK SSQETLLDVN SRKSLVDVPG KKIQSYMPSL RKESSASHVE 250 260 270 280 290 300 QRNENLEGSS AEANEETEDP VNIDEKPPPL AGTNVMNIIL VASECAPWSK TGGLGDVAGA 310 320 330 340 350 360 LPKALARRGH RVMVVAPRYD NYPEPQDSGV RKIYKVDGQD VEVTYFQAFI DGVDFVFIDS 370 380 390 400 410 420 HMFRHIGNNI YGGNRVDILK RMVLFCKAAI EVPWHVPCGG VCYGDGNLVF IANDWHTALL 430 440 450 460 470 480 PVYLKAYYRD NGIMNYTRSV LVIHNIAHQG RGPLEDFSYV DLPPHYMDPF KLYDPVGGEH 490 500 510 520 530 540 FNIFAAGLKT ADRVVTVSHG YSWELKTSQG GWGLHQIINE NDWKLQGIVN GIDTKEWNPE 550 560 570 580 590 600 LDVHLQSDGY MNYSLDTLQT GKPQCKAALQ KELGLPVRDD VPLIGFIGRL DPQKGVDLIA 610 620 630 640 650 660 EAVPWMMGQD VQLVMLGTGR RDLEQMLRQF ECQHNDKIRG WVGFSVKTSH RITAGADILL 670 680 690 700 710 720 MPSRFEPCGL NQLYAMKYGT IPVVHAVGGL RDTVQPFDPF NESGLGWTFS RAEASQLIHA 730 740 750 760 LGNCLLTYRE YKKSWEGIQT RCMTQDLSWD NAAQNYEEVL IAAKYQW

References

[1]	"Biochemical and molecular characterization of a novel starch synthase from potato tubers." Edwards A., Marshall J., Sidebottom C., Visser R.G.F., Smith A.M., Martin C. Plant J. 8:283-294(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 46-55, VARIANT ASP-51. Strain: cv. Desiree. Tissue: Tuber.
[2]	"Resequencing, expression and purification of His-tagged potato soluble starch synthase II in Escherichia coli." Shearer N., Edwards E.A., Martin C., Bornemann S. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Cloning and functional analysis of a cDNA encoding a starch synthase from potato (Solanum tuberosum L.) that is predominantly expressed in leaf tissue." Kossmann J., Abel G.J.W., Springer F., Lloyd J.R., Willmitzer L. Planta 208:503-511(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87988 mRNA. Translation: CAA61241.2.
PIR	T07667.
3D structure databases
ProteinModelPortal	Q43847.
ModBase	Search...
Protein family/group databases
CAZy	GT5. Glycosyltransferase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	PGSC0003DMT400003356; PGSC0003DMT400003356; PGSC0003DMG400001328.
Enzyme and pathway databases
UniPathway	UPA00152.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. IPR011835. Glycogen/starch_synth. IPR013534. Starch_synth_cat_dom. [Graphical view]
Pfam	PF08323. Glyco_transf_5. 1 hit. PF00534. Glycos_transf_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR02095. glgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SSY2_SOLTU

Accession

Primary (citable) accession number: Q43847

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program