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Q43846 (SSY3_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Soluble starch synthase 3, chloroplastic/amyloplastic

EC=2.4.1.21
Alternative name(s):
Soluble starch synthase III
Short name=SS III
Gene names
Name:SS3
Synonyms:SSIII
OrganismSolanum tuberosum (Potato) [Reference proteome]
Taxonomic identifier4113 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum

Protein attributes

Sequence length1230 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May account for most of the soluble starch synthase activity in the tubers. Contributes only a tiny percentage of the granule-bound activity, but may also contribute to the deposition of transient starch in chloroplasts of leaves. HAMAP-Rule MF_00484

Catalytic activity

ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1). HAMAP-Rule MF_00484

Pathway

Glycan biosynthesis; starch biosynthesis. HAMAP-Rule MF_00484

Subcellular location

Plastidchloroplast By similarity. Plastidamyloplast By similarity. Note: Amyloplast or chloroplast, granule-bound and soluble By similarity. HAMAP-Rule MF_00484

Tissue specificity

Tuber, sink and source leaves.

Induction

By sucrose under illumination. HAMAP-Rule MF_00484

Sequence similarities

Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.

Ontologies

Keywords
   Biological processStarch biosynthesis
   Cellular componentAmyloplast
Chloroplast
Plastid
   DomainTransit peptide
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processstarch biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentamyloplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

chloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionstarch binding

Inferred from electronic annotation. Source: InterPro

starch synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6060Chloroplast Potential
Chain61 – 12301170Soluble starch synthase 3, chloroplastic/amyloplastic HAMAP-Rule MF_00484
PRO_0000011146

Sites

Binding site7941ADP-glucose By similarity

Experimental info

Sequence conflict101S → P in CAA64173. Ref.2
Sequence conflict281I → F in CAA64173. Ref.2
Sequence conflict58 – 592SI → PF in CAA64173. Ref.2
Sequence conflict631F → L in CAA64173. Ref.2
Sequence conflict741P → T in CAA64173. Ref.2
Sequence conflict1081K → Q in CAA64173. Ref.2
Sequence conflict1371G → V in CAA64173. Ref.2
Sequence conflict1951G → D Ref.2
Sequence conflict1971I → L Ref.2
Sequence conflict10781N → D in CAA64173. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q43846 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3D95524F3DD9349E

FASTA1,230139,111
        10         20         30         40         50         60 
MDVPFPLHRS LSCTSVSNAI THLKIKPILG FVSHGTTSLS VQSSSWRKDG MVTGVSFSIC 

        70         80         90        100        110        120 
ANFSGRRRRK VSTPRSQGSS PKGFVPRKPS GMSTQRKVQK SNGDKESKST STSKESEISN 

       130        140        150        160        170        180 
QKTVEARVET SDDDTKGVVR DHKFLEDEDE INGSTKSISM SPVRVSSQFV ESEETGGDDK 

       190        200        210        220        230        240 
DAVKLNKSKR SEESGFIIDS VIREQSGSQG ETNASSKGSH AVGTKLYEIL QVDVEPQQLK 

       250        260        270        280        290        300 
ENNAGNVEYK GPVASKLLEI TKASDVEHTE SNEIDDLDTN SFFKSDLIEE DEPLAAGTVE 

       310        320        330        340        350        360 
TGDSSLNLRL EMEANLRRQA IERLAEENLL QGIRLFCFPE VVKPDEDVEI FLNRGLSTLK 

       370        380        390        400        410        420 
NESDVLIMGA FNEWRYRSFT TRLTETHLNG DWWSCKIHVP KEAYRADFVF FNGQDVYDNN 

       430        440        450        460        470        480 
DGNDFSITVK GGMQIIDFEN FLLEEKWREQ EKLAKEQAER ERLAEEQRRI EAEKAEIEAD 

       490        500        510        520        530        540 
RAQAKEEAAK KKKVLRELMV KATKTRDITW YIEPSEFKCE DKVRLYYNKS SGPLSHAKDL 

       550        560        570        580        590        600 
WIHGGYNNWK DGLSIVKKLV KSERIDGDWW YTEVVIPDQA LFLDWVFADG PPKHAIAYDN 

       610        620        630        640        650        660 
NHRQDFHAIV PNHIPEELYW VEEEHQIFKT LQEERRLREA AMRAKVEKTA LLKTETKERT 

       670        680        690        700        710        720 
MKSFLLSQKH VVYTEPLDIQ AGSSVTVYYN PANTVLNGKP EIWFRCSFNR WTHRLGPLPP 

       730        740        750        760        770        780 
QKMSPAENGT HVRATVKVPL DAYMMDFVFS EREDGGIFDN KSGMDYHIPV FGGVAKEPPM 

       790        800        810        820        830        840 
HIVHIAVEMA PIAKVGGLGD VVTSLSRAVQ DLNHNVDIIL PKYDCLKMNN VKDFRFHKNY 

       850        860        870        880        890        900 
FWGGTEIKVW FGKVEGLSVY FLEPQNGLFS KGCVYGCSND GERFGFFCHA ALEFLLQGGF 

       910        920        930        940        950        960 
SPDIIHCHDW SSAPVAWLFK EQYTHYGLSK SRIVFTIHNL EFGADLIGRA MTNADKATTV 

       970        980        990       1000       1010       1020 
SPTYSQEVSG NPVIAPHLHK FHGIVNGIDP DIWDPLNDKF IPIPYTSENV VEGKTAAKEA 

      1030       1040       1050       1060       1070       1080 
LQRKLGLKQA DLPLVGIITR LTHQKGIHLI KHAIWRTLER NGQVVLLGSA PDPRVQNNFV 

      1090       1100       1110       1120       1130       1140 
NLANQLHSKY NDRARLCLTY DEPLSHLIYA GADFILVPSI FEPCGLTQLT AMRYGSIPVV 

      1150       1160       1170       1180       1190       1200 
RKTGGLYDTV FDVDHDKERA QQCGLEPNGF SFDGADAGGV DYALNRALSA WYDGRDWFNS 

      1210       1220       1230 
LCKQVMEQDW SWNRPALDYL ELYHAARKLE 

« Hide

References

[1]"Identification of the major starch synthase in the soluble fraction of potato tubers."
Marshall J., Sidebottom C., Debet M., Martin C., Smith A.M., Edwards A.
Plant Cell 8:1121-1135(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 767-773 AND 1000-1014.
Strain: cv. Desiree.
Tissue: Tuber.
[2]"Cloning and functional analysis of a cDNA encoding a novel 139 kDa starch synthase from potato (Solanum tuberosum L.)."
Abel G.J.W., Springer F., Willmitzer L., Kossmann J.
Plant J. 10:981-991(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Desiree.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X95759 mRNA. Translation: CAA65065.1.
X94400 mRNA. Translation: CAA64173.1.
PIRT07663.
UniGeneStu.198.

3D structure databases

ProteinModelPortalQ43846.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM53. Carbohydrate-Binding Module Family 53.
GT5. Glycosyltransferase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-1884.
UniPathwayUPA00152.

Family and domain databases

HAMAPMF_00484. Glycogen_synth.
InterProIPR005085. CBM_fam25.
IPR001296. Glyco_trans_1.
IPR011835. Glycogen/starch_synth.
IPR013534. Starch_synth_cat_dom.
[Graphical view]
PfamPF03423. CBM_25. 3 hits.
PF08323. Glyco_transf_5. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
SMARTSM01066. CBM_25. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSSY3_SOLTU
AccessionPrimary (citable) accession number: Q43846
Secondary accession number(s): O04842
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways