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Protein

Ribulose-phosphate 3-epimerase, chloroplastic

Gene
N/A
Organism
Solanum tuberosum (Potato)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.

Catalytic activityi

D-ribulose 5-phosphate = D-xylulose 5-phosphate.

Cofactori

Co2+By similarity, Fe2+By similarity, Mn2+By similarity, Zn2+By similarityNote: Binds 1 divalent metal cation per subunit. Active with Co2+, Fe2+, Mn2+ and Zn2+.By similarity

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62SubstrateBy similarity1
Metal bindingi87Divalent metal cationBy similarity1
Active sitei89Proton acceptorBy similarity1
Metal bindingi89Divalent metal cationBy similarity1
Metal bindingi120Divalent metal cationBy similarity1
Binding sitei120SubstrateBy similarity1
Active sitei231Proton donorBy similarity1
Metal bindingi231Divalent metal cationBy similarity1
Binding sitei233Substrate; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Calvin cycle, Carbohydrate metabolism, Pentose shunt

Keywords - Ligandi

Cobalt, Iron, Manganese, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose-phosphate 3-epimerase, chloroplastic (EC:5.1.3.1)
Alternative name(s):
Pentose-5-phosphate 3-epimerase
Short name:
PPE
R5P3E
Short name:
RPE
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
Proteomesi
  • UP000011115 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei‹1 – 45ChloroplastAdd BLAST›45
ChainiPRO_000002541746 – 280Ribulose-phosphate 3-epimerase, chloroplasticAdd BLAST235

Proteomic databases

PRIDEiQ43843.

Expressioni

Tissue specificityi

Highest level of expression in leaves, whereas it is low in roots, tubers, and stems.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

STRINGi4113.PGSC0003DMT400050256.

Structurei

Secondary structure

1280
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi48 – 51Combined sources4
Beta strandi58 – 62Combined sources5
Helixi63 – 65Combined sources3
Helixi68 – 70Combined sources3
Helixi71 – 80Combined sources10
Beta strandi86 – 97Combined sources12
Helixi103 – 109Combined sources7
Helixi110 – 112Combined sources3
Beta strandi117 – 125Combined sources9
Helixi126 – 135Combined sources10
Beta strandi139 – 144Combined sources6
Turni147 – 149Combined sources3
Helixi153 – 162Combined sources10
Beta strandi165 – 171Combined sources7
Helixi177 – 180Combined sources4
Turni181 – 186Combined sources6
Beta strandi188 – 195Combined sources8
Helixi208 – 222Combined sources15
Beta strandi227 – 233Combined sources7
Turni236 – 238Combined sources3
Helixi239 – 245Combined sources7
Beta strandi249 – 253Combined sources5
Helixi254 – 257Combined sources4
Helixi262 – 270Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RPXX-ray2.30A/B/C47-276[»]
ProteinModelPortaliQ43843.
SMRiQ43843.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ43843.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni198 – 201Substrate bindingBy similarity4
Regioni253 – 254Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3111. Eukaryota.
COG0036. LUCA.

Family and domain databases

CDDicd00429. RPE. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PANTHERiPTHR11749. PTHR11749. 1 hit.
PfamiPF00834. Ribul_P_3_epim. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01163. rpe. 1 hit.
PROSITEiPS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SLGSSTLLQS QISGFGGSQK LQKISFSNPN SLTFTRRRIQ TVVNASSRVD
60 70 80 90 100
KFSKSDIIVS PSILSANFSK LGEQVKAIEQ AGCDWIHVDV MDGRFVPNIT
110 120 130 140 150
IGPLVVDSLR PITDLPLDVH LMIVEPDQRV PDFIKAGADI VSVHCEQSST
160 170 180 190 200
IHLHRTINQI KSLGAKAGVV LNPGTPLTAI EYVLDAVDLV LIMSVNPGFG
210 220 230 240 250
GQSFIESQVK KISDLRKICA ERGLNPWIEV DGGVGPKNAY KVIEAGANAL
260 270 280
VAGSAVFGAP DYAEAIKGIK TSKRPEAVAV
Length:280
Mass (Da):29,881
Last modified:November 1, 1997 - v1
Checksum:i3381A798F056491C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50098 mRNA. Translation: CAA90426.1.
PIRiS68407.
UniGeneiStu.14340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50098 mRNA. Translation: CAA90426.1.
PIRiS68407.
UniGeneiStu.14340.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RPXX-ray2.30A/B/C47-276[»]
ProteinModelPortaliQ43843.
SMRiQ43843.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4113.PGSC0003DMT400050256.

Proteomic databases

PRIDEiQ43843.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG3111. Eukaryota.
COG0036. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00116.

Miscellaneous databases

EvolutionaryTraceiQ43843.

Family and domain databases

CDDicd00429. RPE. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PANTHERiPTHR11749. PTHR11749. 1 hit.
PfamiPF00834. Ribul_P_3_epim. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01163. rpe. 1 hit.
PROSITEiPS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPE_SOLTU
AccessioniPrimary (citable) accession number: Q43843
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.