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Q43843

- RPE_SOLTU

UniProt

Q43843 - RPE_SOLTU

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Protein
Ribulose-phosphate 3-epimerase, chloroplastic
Gene
N/A
Organism
Solanum tuberosum (Potato)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.

Catalytic activityi

D-ribulose 5-phosphate = D-xylulose 5-phosphate.

Cofactori

Binds 1 divalent metal cation per subunit. Active with Co2+, Fe2+, Mn2+ and Zn2+ By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621Substrate By similarity
Metal bindingi87 – 871Divalent metal cation By similarity
Active sitei89 – 891Proton acceptor By similarity
Metal bindingi89 – 891Divalent metal cation By similarity
Metal bindingi120 – 1201Divalent metal cation By similarity
Binding sitei120 – 1201Substrate By similarity
Active sitei231 – 2311Proton donor By similarity
Metal bindingi231 – 2311Divalent metal cation By similarity
Binding sitei233 – 2331Substrate; via amide nitrogen By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribulose-phosphate 3-epimerase activity Source: UniProtKB

GO - Biological processi

  1. pentose-phosphate shunt Source: UniProtKB-KW
  2. reductive pentose-phosphate cycle Source: UniProtKB-UniPathway
  3. response to cold Source: EnsemblPlants/Gramene
  4. response to nematode Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Calvin cycle, Carbohydrate metabolism, Pentose shunt

Keywords - Ligandi

Cobalt, Iron, Manganese, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose-phosphate 3-epimerase, chloroplastic (EC:5.1.3.1)
Alternative name(s):
Pentose-5-phosphate 3-epimerase
Short name:
PPE
R5P3E
Short name:
RPE
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
ProteomesiUP000011115: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: EnsemblPlants/Gramene
  2. chloroplast envelope Source: EnsemblPlants/Gramene
  3. chloroplast stroma Source: EnsemblPlants/Gramene
  4. chloroplast thylakoid membrane Source: UniProtKB
  5. stromule Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei‹1 – 45›45Chloroplast
Add
BLAST
Chaini46 – 280235Ribulose-phosphate 3-epimerase, chloroplastic
PRO_0000025417Add
BLAST

Proteomic databases

PRIDEiQ43843.

Expressioni

Tissue specificityi

Highest level of expression in leaves, whereas it is low in roots, tubers, and stems.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi48 – 514
Beta strandi58 – 625
Helixi63 – 653
Helixi68 – 703
Helixi71 – 8010
Beta strandi86 – 9712
Helixi103 – 1097
Helixi110 – 1123
Beta strandi117 – 1259
Helixi126 – 13510
Beta strandi139 – 1446
Turni147 – 1493
Helixi153 – 16210
Beta strandi165 – 1717
Helixi177 – 1804
Turni181 – 1866
Beta strandi188 – 1958
Helixi208 – 22215
Beta strandi227 – 2337
Turni236 – 2383
Helixi239 – 2457
Beta strandi249 – 2535
Helixi254 – 2574
Helixi262 – 2709

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RPXX-ray2.30A/B/C47-276[»]
ProteinModelPortaliQ43843.
SMRiQ43843. Positions 47-276.

Miscellaneous databases

EvolutionaryTraceiQ43843.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni198 – 2014Substrate binding By similarity
Regioni253 – 2542Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PANTHERiPTHR11749. PTHR11749. 1 hit.
PfamiPF00834. Ribul_P_3_epim. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01163. rpe. 1 hit.
PROSITEiPS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43843-1 [UniParc]FASTAAdd to Basket

« Hide

SLGSSTLLQS QISGFGGSQK LQKISFSNPN SLTFTRRRIQ TVVNASSRVD    50
KFSKSDIIVS PSILSANFSK LGEQVKAIEQ AGCDWIHVDV MDGRFVPNIT 100
IGPLVVDSLR PITDLPLDVH LMIVEPDQRV PDFIKAGADI VSVHCEQSST 150
IHLHRTINQI KSLGAKAGVV LNPGTPLTAI EYVLDAVDLV LIMSVNPGFG 200
GQSFIESQVK KISDLRKICA ERGLNPWIEV DGGVGPKNAY KVIEAGANAL 250
VAGSAVFGAP DYAEAIKGIK TSKRPEAVAV 280
Length:280
Mass (Da):29,881
Last modified:November 1, 1997 - v1
Checksum:i3381A798F056491C
GO

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50098 mRNA. Translation: CAA90426.1.
PIRiS68407.
UniGeneiStu.14340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50098 mRNA. Translation: CAA90426.1 .
PIRi S68407.
UniGenei Stu.14340.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1RPX X-ray 2.30 A/B/C 47-276 [» ]
ProteinModelPortali Q43843.
SMRi Q43843. Positions 47-276.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q43843.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00116 .

Miscellaneous databases

EvolutionaryTracei Q43843.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view ]
PANTHERi PTHR11749. PTHR11749. 1 hit.
Pfami PF00834. Ribul_P_3_epim. 1 hit.
[Graphical view ]
SUPFAMi SSF51366. SSF51366. 1 hit.
TIGRFAMsi TIGR01163. rpe. 1 hit.
PROSITEi PS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Chloroplast pentose-5-phosphate 3-epimerase from potato: cloning, cDNA sequence, and tissue-specific enzyme accumulation."
    Teige M., Kopriva S., Bauwe H., Suess K.-H.
    FEBS Lett. 377:349-352(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Desiree.
    Tissue: Leaf.
  2. "Structure and mechanism of the amphibolic enzyme D-ribulose-5-phosphate 3-epimerase from potato chloroplasts."
    Kopp J., Kopriva S., Suess K.-H., Schulz G.E.
    J. Mol. Biol. 287:761-771(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiRPE_SOLTU
AccessioniPrimary (citable) accession number: Q43843
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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