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Q43832

- RBS2_SPIOL

UniProt

Q43832 - RBS2_SPIOL

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Protein
Ribulose bisphosphate carboxylase small chain 2, chloroplastic
Gene
RBCS2
Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

GO - Molecular functioni

  1. monooxygenase activity Source: UniProtKB-KW
  2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. carbon fixation Source: UniProtKB-KW
  2. photorespiration Source: UniProtKB-KW
  3. photosynthesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Carbon dioxide fixation, Photorespiration, Photosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase small chain 2, chloroplastic (EC:4.1.1.39)
Short name:
RuBisCO small subunit 2
Gene namesi
Name:RBCS2
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5757Chloroplast By similarity
Add
BLAST
Chaini58 – 180123Ribulose bisphosphate carboxylase small chain 2, chloroplastic
PRO_0000031558Add
BLAST

Interactioni

Subunit structurei

8 large chains + 8 small chains.

Protein-protein interaction databases

IntActiQ43832. 1 interaction.
MINTiMINT-1522619.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni71 – 744
Helixi80 – 9213
Beta strandi96 – 1049
Beta strandi125 – 1284
Helixi137 – 15014
Beta strandi154 – 1629
Turni163 – 1664
Beta strandi167 – 1759

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AA1X-ray2.20C/F/I/S58-180[»]
1AUSX-ray2.20S/T/U/V58-180[»]
1IR1X-ray1.80S/T/U/V58-180[»]
1UPMX-ray2.30C/F/I/M/P/S/T/W58-180[»]
1UPPX-ray2.30I/J/K/L58-180[»]
1UZDX-ray2.40C/F/I/J/M/P/T/W103-121[»]
ProteinModelPortaliQ43832.
SMRiQ43832. Positions 59-180.

Miscellaneous databases

EvolutionaryTraceiQ43832.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.30.190.10. 1 hit.
InterProiIPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
IPR024680. RuBisCO_ssu_N.
[Graphical view]
PfamiPF12338. RbcS. 1 hit.
PF00101. RuBisCO_small. 1 hit.
[Graphical view]
PRINTSiPR00152. RUBISCOSMALL.
SMARTiSM00961. RuBisCO_small. 1 hit.
[Graphical view]
SUPFAMiSSF55239. SSF55239. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43832-1 [UniParc]FASTAAdd to Basket

« Hide

MASSVLSSAA VATVSRTPAQ ASMVAPFTGL KSTVGFPATK KNDDITSLAS    50
NGGRVQCMKV WPTQNMKRYE TLSYLPPLTT DQLARQVDYL LNNKWVPCLE 100
FETDHGFVYR EHHNSPGYYD GRYWTMWKLP MFGCTDPAQV LNELEECKKE 150
YPNAFIRIIG FDSNRQVQCV SFIAYKPAGY 180
Length:180
Mass (Da):20,326
Last modified:November 1, 1996 - v1
Checksum:i651FF2F212CFD64A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X97600 mRNA. Translation: CAA66201.1.
PIRiS78083.

Cross-referencesi

Web resourcesi

Protein Spotlight

The Plant Kingdom's sloth - Issue 38 of September 2003

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X97600 mRNA. Translation: CAA66201.1 .
PIRi S78083.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AA1 X-ray 2.20 C/F/I/S 58-180 [» ]
1AUS X-ray 2.20 S/T/U/V 58-180 [» ]
1IR1 X-ray 1.80 S/T/U/V 58-180 [» ]
1UPM X-ray 2.30 C/F/I/M/P/S/T/W 58-180 [» ]
1UPP X-ray 2.30 I/J/K/L 58-180 [» ]
1UZD X-ray 2.40 C/F/I/J/M/P/T/W 103-121 [» ]
ProteinModelPortali Q43832.
SMRi Q43832. Positions 59-180.
ModBasei Search...

Protein-protein interaction databases

IntActi Q43832. 1 interaction.
MINTi MINT-1522619.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q43832.

Family and domain databases

Gene3Di 3.30.190.10. 1 hit.
InterProi IPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
IPR024680. RuBisCO_ssu_N.
[Graphical view ]
Pfami PF12338. RbcS. 1 hit.
PF00101. RuBisCO_small. 1 hit.
[Graphical view ]
PRINTSi PR00152. RUBISCOSMALL.
SMARTi SM00961. RuBisCO_small. 1 hit.
[Graphical view ]
SUPFAMi SSF55239. SSF55239. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning and expression of a ribulose-1,5-bisphophate carboxylase small subunit precursor in Spinacia oleracea."
    Diogon T., Capelli N., Greppin H., Simon P.
    Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nobel.
    Tissue: Leaf.
  2. "Reexamination of the three-dimensional structure of the small subunit of RuBisCo from higher plants."
    Knight S., Andersson I., Braenden C.-I.
    Science 244:702-705(1989)
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
  3. "Crystallographic analysis of ribulose 1,5-bisphosphate carboxylase from spinach at 2.4-A resolution. Subunit interactions and active site."
    Knight S., Andersson I., Braenden C.-I.
    J. Mol. Biol. 215:113-160(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
  4. "A common structural basis for the inhibition of ribulose 1,5-bisphosphate carboxylase by 4-carboxyarabinitol 1,5-bisphosphate and xylulose 1,5-bisphosphate."
    Taylor T.C., Fothergill M.D., Andersson I.
    J. Biol. Chem. 271:32894-32899(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 13-123 IN COMPLEX WITH 2 INHIBITORS (4-CABP AND XUBP).
  5. "Large structures at high resolution: the 1.6-A crystal structure of spinach ribulose-1,5-bisphosphate carboxylase/oxygenase complexed with 2-carboxyarabinitol bisphosphate."
    Andersson I.
    J. Mol. Biol. 259:160-174(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) IN COMPLEX WITH THE TRANSITION-STATE ANALOGUE 2-CABP.
  6. "The structure of the complex between rubisco and its natural substrate ribulose 1,5-bisphosphate."
    Taylor T.C., Andersson I.
    J. Mol. Biol. 265:432-444(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH ITS NATURAL SUBSTRATE.
  7. "Crystal structure of activated ribulose-1,5-bisphosphate carboxylase/oxygenase from green alga Chlamydomonas reinhardtii complexed with 2-carboxyarabinitol-1,5-bisphosphate."
    Mizohata E., Matsumura H., Okano Y., Kumei M., Takuma H., Onodera J., Kato K., Shibata N., Inoue T., Yokota A., Kai Y.
    J. Mol. Biol. 316:679-691(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH THE TRANSITION-STATE ANALOGUE 2-CABP.

Entry informationi

Entry nameiRBS2_SPIOL
AccessioniPrimary (citable) accession number: Q43832
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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