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Q43832

- RBS2_SPIOL

UniProt

Q43832 - RBS2_SPIOL

Protein

Ribulose bisphosphate carboxylase small chain 2, chloroplastic

Gene

RBCS2

Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

    GO - Molecular functioni

    1. monooxygenase activity Source: UniProtKB-KW
    2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbon fixation Source: UniProtKB-KW
    2. photorespiration Source: UniProtKB-KW
    3. photosynthesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase, Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Carbon dioxide fixation, Photorespiration, Photosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylase small chain 2, chloroplastic (EC:4.1.1.39)
    Short name:
    RuBisCO small subunit 2
    Gene namesi
    Name:RBCS2
    OrganismiSpinacia oleracea (Spinach)
    Taxonomic identifieri3562 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5757ChloroplastBy similarityAdd
    BLAST
    Chaini58 – 180123Ribulose bisphosphate carboxylase small chain 2, chloroplasticPRO_0000031558Add
    BLAST

    Interactioni

    Subunit structurei

    8 large chains + 8 small chains.4 Publications

    Protein-protein interaction databases

    IntActiQ43832. 1 interaction.
    MINTiMINT-1522619.

    Structurei

    Secondary structure

    1
    180
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni71 – 744
    Helixi80 – 9213
    Beta strandi96 – 1049
    Beta strandi125 – 1284
    Helixi137 – 15014
    Beta strandi154 – 1629
    Turni163 – 1664
    Beta strandi167 – 1759

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AA1X-ray2.20C/F/I/S58-180[»]
    1AUSX-ray2.20S/T/U/V58-180[»]
    1IR1X-ray1.80S/T/U/V58-180[»]
    1UPMX-ray2.30C/F/I/M/P/S/T/W58-180[»]
    1UPPX-ray2.30I/J/K/L58-180[»]
    1UZDX-ray2.40C/F/I/J/M/P/T/W103-121[»]
    ProteinModelPortaliQ43832.
    SMRiQ43832. Positions 59-180.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ43832.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuBisCO small chain family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.30.190.10. 1 hit.
    InterProiIPR024681. RuBisCO_sc.
    IPR000894. RuBisCO_sc_dom.
    IPR024680. RuBisCO_ssu_N.
    [Graphical view]
    PfamiPF12338. RbcS. 1 hit.
    PF00101. RuBisCO_small. 1 hit.
    [Graphical view]
    PRINTSiPR00152. RUBISCOSMALL.
    SMARTiSM00961. RuBisCO_small. 1 hit.
    [Graphical view]
    SUPFAMiSSF55239. SSF55239. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q43832-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASSVLSSAA VATVSRTPAQ ASMVAPFTGL KSTVGFPATK KNDDITSLAS    50
    NGGRVQCMKV WPTQNMKRYE TLSYLPPLTT DQLARQVDYL LNNKWVPCLE 100
    FETDHGFVYR EHHNSPGYYD GRYWTMWKLP MFGCTDPAQV LNELEECKKE 150
    YPNAFIRIIG FDSNRQVQCV SFIAYKPAGY 180
    Length:180
    Mass (Da):20,326
    Last modified:November 1, 1996 - v1
    Checksum:i651FF2F212CFD64A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97600 mRNA. Translation: CAA66201.1.
    PIRiS78083.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    The Plant Kingdom's sloth - Issue 38 of September 2003

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97600 mRNA. Translation: CAA66201.1 .
    PIRi S78083.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AA1 X-ray 2.20 C/F/I/S 58-180 [» ]
    1AUS X-ray 2.20 S/T/U/V 58-180 [» ]
    1IR1 X-ray 1.80 S/T/U/V 58-180 [» ]
    1UPM X-ray 2.30 C/F/I/M/P/S/T/W 58-180 [» ]
    1UPP X-ray 2.30 I/J/K/L 58-180 [» ]
    1UZD X-ray 2.40 C/F/I/J/M/P/T/W 103-121 [» ]
    ProteinModelPortali Q43832.
    SMRi Q43832. Positions 59-180.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q43832. 1 interaction.
    MINTi MINT-1522619.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q43832.

    Family and domain databases

    Gene3Di 3.30.190.10. 1 hit.
    InterProi IPR024681. RuBisCO_sc.
    IPR000894. RuBisCO_sc_dom.
    IPR024680. RuBisCO_ssu_N.
    [Graphical view ]
    Pfami PF12338. RbcS. 1 hit.
    PF00101. RuBisCO_small. 1 hit.
    [Graphical view ]
    PRINTSi PR00152. RUBISCOSMALL.
    SMARTi SM00961. RuBisCO_small. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55239. SSF55239. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and expression of a ribulose-1,5-bisphophate carboxylase small subunit precursor in Spinacia oleracea."
      Diogon T., Capelli N., Greppin H., Simon P.
      Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Nobel.
      Tissue: Leaf.
    2. "Reexamination of the three-dimensional structure of the small subunit of RuBisCo from higher plants."
      Knight S., Andersson I., Braenden C.-I.
      Science 244:702-705(1989)
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    3. "Crystallographic analysis of ribulose 1,5-bisphosphate carboxylase from spinach at 2.4-A resolution. Subunit interactions and active site."
      Knight S., Andersson I., Braenden C.-I.
      J. Mol. Biol. 215:113-160(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
    4. "A common structural basis for the inhibition of ribulose 1,5-bisphosphate carboxylase by 4-carboxyarabinitol 1,5-bisphosphate and xylulose 1,5-bisphosphate."
      Taylor T.C., Fothergill M.D., Andersson I.
      J. Biol. Chem. 271:32894-32899(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 13-123 IN COMPLEX WITH 2 INHIBITORS (4-CABP AND XUBP).
    5. "Large structures at high resolution: the 1.6-A crystal structure of spinach ribulose-1,5-bisphosphate carboxylase/oxygenase complexed with 2-carboxyarabinitol bisphosphate."
      Andersson I.
      J. Mol. Biol. 259:160-174(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) IN COMPLEX WITH THE TRANSITION-STATE ANALOGUE 2-CABP.
    6. "The structure of the complex between rubisco and its natural substrate ribulose 1,5-bisphosphate."
      Taylor T.C., Andersson I.
      J. Mol. Biol. 265:432-444(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH ITS NATURAL SUBSTRATE.
    7. "Crystal structure of activated ribulose-1,5-bisphosphate carboxylase/oxygenase from green alga Chlamydomonas reinhardtii complexed with 2-carboxyarabinitol-1,5-bisphosphate."
      Mizohata E., Matsumura H., Okano Y., Kumei M., Takuma H., Onodera J., Kato K., Shibata N., Inoue T., Yokota A., Kai Y.
      J. Mol. Biol. 316:679-691(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH THE TRANSITION-STATE ANALOGUE 2-CABP.

    Entry informationi

    Entry nameiRBS2_SPIOL
    AccessioniPrimary (citable) accession number: Q43832
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3