Reviewed, UniProtKB/Swiss-Prot Q43827 (MASY_RAPSA)

Names and origin

Protein names

Recommended name:
    Malate synthase, glyoxysomal
    EC=2.3.3.9

Gene names

Name:

MLS

Organism

Raphanus sativus (Radish)

Taxonomic identifier

3726 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Raphanus

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Subcellular location	Glyoxysome By similarity.
Sequence similarities	Belongs to the malate synthase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Molecular function	Transferase
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Malate synthase, glyoxysomal		PRO_0000166871
Regions
	Motif	564 – 566	3	Microbody targeting signal Potential
Sites
	Active site	179	1	Proton acceptor By similarity
	Active site	465	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43827-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 807104112623FF77	FASTA	566	64,138
	10 20 30 40 50 60 MELETSVYRP NAAVYDSPDG VEVRGRYDQV FAKILTREAL GFVAELQRDV SLGMLRYAWS 70 80 90 100 110 120 AVERQNVVTT FVLSLGLTLP LSSSEIGEWV CSVCSPPAVA DRRVEITGPV ERKMIINALN 130 140 150 160 170 180 SGAKVFMADF EDALSPSWEN LMKGQVNLKD AVDGTITFHD KARNKVYKLN DQVAKLFVRP 190 200 210 220 230 240 RGWHLPEAHI LIDGEPAIGC LVDFGLYFFH NYSKFRQTQG SGYGPFFYLP KMEHSREAKI 250 260 270 280 290 300 WNSVFERAEK MAGIERGSIR ATVLIETLPA VFQMNEILYE LRDHSVGLNC GRWDYIFSYV 310 320 330 340 350 360 KTFQAHPDRL LPDRVLVGMG QHFMRSYSDL LIRTCHKRGV HAWEGMAAQI AIRDDPKAND 370 380 390 400 410 420 MALDLVKKDK LRQVRAGHDG TWAAHPGLIP ICMDAFGHMG KNPNQIKSMK RDDASAITEE 430 440 450 460 470 480 DLLQIPRGVR TLDGLRLNTR VGIQYLAAWL TGSGSVPLYN LTDEDAATAE ISRVQNWQWI 490 500 510 520 530 540 RYGVELKRRT GLEVRVSKEL FGRVVEEEME RIEKEVGKER SLREECIRKL ARCLQSSVTA 550 560 AELDDFLTLA VYDHIVAHYP INVSRL

References

[1]	"Overexpression of radish malate synthase in yeast." Vollack K.U., Bach T.J. Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Saxa Knacker. Tissue: Seedling.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X78852 mRNA. Translation: CAA55407.1.
PIR	S44186.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.3.9. 1363.
Family and domain databases
InterPro	IPR011076. Malate_synth-like_core. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 2 hits. [Graphical view]
PIRSF	PIRSF001363. Malate_synth. 1 hit.
TIGRFAMs	TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MASY_RAPSA

Accession

Primary (citable) accession number: Q43827

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	October 13, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)