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Protein

Malate synthase, glyoxysomal

Gene

MLS

Organism
Raphanus sativus (Radish)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei179 – 1791Proton acceptorBy similarity
Active sitei465 – 4651Proton donorBy similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-UniPathway
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase, glyoxysomal (EC:2.3.3.9)
Gene namesi
Name:MLS
OrganismiRaphanus sativus (Radish)
Taxonomic identifieri3726 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeRaphanus

Subcellular locationi

  1. Glyoxysome By similarity

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566Malate synthase, glyoxysomalPRO_0000166871Add
BLAST

Proteomic databases

PRIDEiQ43827.

Structurei

3D structure databases

ProteinModelPortaliQ43827.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi564 – 5663Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q43827-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELETSVYRP NAAVYDSPDG VEVRGRYDQV FAKILTREAL GFVAELQRDV
60 70 80 90 100
SLGMLRYAWS AVERQNVVTT FVLSLGLTLP LSSSEIGEWV CSVCSPPAVA
110 120 130 140 150
DRRVEITGPV ERKMIINALN SGAKVFMADF EDALSPSWEN LMKGQVNLKD
160 170 180 190 200
AVDGTITFHD KARNKVYKLN DQVAKLFVRP RGWHLPEAHI LIDGEPAIGC
210 220 230 240 250
LVDFGLYFFH NYSKFRQTQG SGYGPFFYLP KMEHSREAKI WNSVFERAEK
260 270 280 290 300
MAGIERGSIR ATVLIETLPA VFQMNEILYE LRDHSVGLNC GRWDYIFSYV
310 320 330 340 350
KTFQAHPDRL LPDRVLVGMG QHFMRSYSDL LIRTCHKRGV HAWEGMAAQI
360 370 380 390 400
AIRDDPKAND MALDLVKKDK LRQVRAGHDG TWAAHPGLIP ICMDAFGHMG
410 420 430 440 450
KNPNQIKSMK RDDASAITEE DLLQIPRGVR TLDGLRLNTR VGIQYLAAWL
460 470 480 490 500
TGSGSVPLYN LTDEDAATAE ISRVQNWQWI RYGVELKRRT GLEVRVSKEL
510 520 530 540 550
FGRVVEEEME RIEKEVGKER SLREECIRKL ARCLQSSVTA AELDDFLTLA
560
VYDHIVAHYP INVSRL
Length:566
Mass (Da):64,138
Last modified:November 1, 1996 - v1
Checksum:i807104112623FF77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78852 mRNA. Translation: CAA55407.1.
PIRiS44186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78852 mRNA. Translation: CAA55407.1.
PIRiS44186.

3D structure databases

ProteinModelPortaliQ43827.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ43827.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Overexpression of radish malate synthase in yeast."
    Vollack K.U., Bach T.J.
    Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Saxa Knacker.
    Tissue: Seedling.

Entry informationi

Entry nameiMASY_RAPSA
AccessioniPrimary (citable) accession number: Q43827
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.