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Q43827 (MASY_RAPSA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 9, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Malate synthase, glyoxysomal
EC=2.3.3.9
Gene names
Name:MLS
OrganismRaphanus sativus (Radish)
Taxonomic identifier3726 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Raphanus

Protein attributes

Sequence length566 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Subcellular location

Glyoxysome By similarity.

Sequence similarities

Belongs to the malate synthase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentGlyoxysome
Peroxisome
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentglyoxysome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 566566Malate synthase, glyoxysomal
PRO_0000166871

Regions

Motif564 – 5663Microbody targeting signal Potential

Sites

Active site1791Proton acceptor By similarity
Active site4651Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q43827 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 807104112623FF77

FASTA56664,138
        10         20         30         40         50         60 
MELETSVYRP NAAVYDSPDG VEVRGRYDQV FAKILTREAL GFVAELQRDV SLGMLRYAWS 

        70         80         90        100        110        120 
AVERQNVVTT FVLSLGLTLP LSSSEIGEWV CSVCSPPAVA DRRVEITGPV ERKMIINALN 

       130        140        150        160        170        180 
SGAKVFMADF EDALSPSWEN LMKGQVNLKD AVDGTITFHD KARNKVYKLN DQVAKLFVRP 

       190        200        210        220        230        240 
RGWHLPEAHI LIDGEPAIGC LVDFGLYFFH NYSKFRQTQG SGYGPFFYLP KMEHSREAKI 

       250        260        270        280        290        300 
WNSVFERAEK MAGIERGSIR ATVLIETLPA VFQMNEILYE LRDHSVGLNC GRWDYIFSYV 

       310        320        330        340        350        360 
KTFQAHPDRL LPDRVLVGMG QHFMRSYSDL LIRTCHKRGV HAWEGMAAQI AIRDDPKAND 

       370        380        390        400        410        420 
MALDLVKKDK LRQVRAGHDG TWAAHPGLIP ICMDAFGHMG KNPNQIKSMK RDDASAITEE 

       430        440        450        460        470        480 
DLLQIPRGVR TLDGLRLNTR VGIQYLAAWL TGSGSVPLYN LTDEDAATAE ISRVQNWQWI 

       490        500        510        520        530        540 
RYGVELKRRT GLEVRVSKEL FGRVVEEEME RIEKEVGKER SLREECIRKL ARCLQSSVTA 

       550        560 
AELDDFLTLA VYDHIVAHYP INVSRL

« Hide

References

[1]"Overexpression of radish malate synthase in yeast."
Vollack K.U., Bach T.J.
Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Saxa Knacker.
Tissue: Seedling.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X78852 mRNA. Translation: CAA55407.1.
PIRS44186.

3D structure databases

ProteinModelPortalQ43827.
ModBaseSearch...

Proteomic databases

PRIDEQ43827.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00703; UER00720.

Family and domain databases

InterProIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PANTHERPTHR21631:SF1. PTHR21631:SF1. 1 hit.
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001363. Malate_synth. 1 hit.
SUPFAMSSF51645. Malat_synth_like. 1 hit.
TIGRFAMsTIGR01344. malate_syn_A. 1 hit.
PROSITEPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMASY_RAPSA
AccessionPrimary (citable) accession number: Q43827
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: January 9, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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