Reviewed, UniProtKB/Swiss-Prot Q43822 (PLSB_PHAVU)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase, chloroplastic
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:

PLSB

Organism

Phaseolus vulgaris (Kidney bean) (French bean)

Taxonomic identifier

3885 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Phaseolus

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate. The enzyme from chilling-resistant plants discriminates against non-fluid palmitic acid and selects oleic acid whereas the enzyme from sensitive plants accepts both fatty acids.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Plastid › chloroplast stroma.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 96	96	Chloroplast Potential
	Chain	97 – 461	365	Glycerol-3-phosphate acyltransferase, chloroplastic		PRO_0000024699
Regions
	Motif	231 – 236	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q43822-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EAC4FC837908B38A	FASTA	461	50,697
	10 20 30 40 50 60 MSMTGSSAYY VAHAIPPFLR LSNKTMLLLS TPPTTFFPTS TTPRVTLLSS TSSSSSSSIS 70 80 90 100 110 120 LRSSTAPSPS CSSVTPKDNC LASAKHSPPN MSASVSSRTF LNAQSEQDVF AGIKKEVEAG 130 140 150 160 170 180 SLPANVAAGM EEVYNNYKKA VIQSGDPKAN EIVLSNMIAL LDRVFLDVTD PFVFQPHHKA 190 200 210 220 230 240 KREPFDYYVF GQNYIRPLVD FKNAYVGNMP LFIEMEEKLK QGHNIILMSN HQTEADPAII 250 260 270 280 290 300 SLLLETRLPY IAENLTYVAG DRVITDPLSK PFSIGRNLIC VYSKKHMLDD PALVEMKRTA 310 320 330 340 350 360 NIRALKEMAM LLRNGSQLVW IAPSGGRDRP DAQTREWVPA PFDISSVDNM RRLVEHSGPP 370 380 390 400 410 420 GHVYPLAILC HDIMPPPLKV EKEIGEKRII CFHGAGISVA PAISFSETTA TCENPEKAKE 430 440 450 460 VFSKALYNSV TEQYNVLKSA IQGKKGFEAS TPVVTLSQPW K

References

[1]	"Cloning and sequencing of a full-length cDNA coding for sn-glycerol-3-phosphate acyltransferase from Phaseolus vulgaris." Fritz M., Heinz E., Wolter F.P. Plant Physiol. 107:1039-1040(1995) [PubMed: 7716242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Annabel. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79722 mRNA. Translation: CAA56159.1.
PIR	T11819.
3D structure databases
SMR	Q43822. Positions 97-460.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.15. 8.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR016222. Glycerol-3-P_O-acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000431. Glycerol-3-P_O-acyltransfrase. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_PHAVU

Accession

Primary (citable) accession number: Q43822

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)