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Q43794 (SYE_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase, chloroplastic/mitochondrial

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length569 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022_B

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022_B

Subcellular location

Plastidchloroplast Potential. Mitochondrion Potential HAMAP-Rule MF_00022_B.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentChloroplast
Mitochondrion
Plastid
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
RNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast and mitochondrion Potential
Chain? – 569Glutamate--tRNA ligase, chloroplastic/mitochondrial HAMAP-Rule MF_00022_BPRO_0000119737

Regions

Nucleotide binding303 – 3075ATP By similarity
Region59 – 613Glutamate binding By similarity
Region247 – 2515Glutamate binding By similarity
Motif62 – 7211"HIGH" region HAMAP-Rule MF_00022_B
Motif303 – 3075"KMSKS" region HAMAP-Rule MF_00022_B

Sites

Binding site691ATP By similarity
Binding site951Glutamate By similarity
Binding site2651Glutamate By similarity
Binding site2681ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q43794 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F2E81D73460A1844

FASTA56963,338
        10         20         30         40         50         60 
MATLAAAPWF RVRLIPELKN SQSLLYCRGN HSYRQSLCSR RRSFSVYASA GDGGDVRVRF 

        70         80         90        100        110        120 
APSPTGNLHV GGARTALFNY LYARAKGGKF ILRIEDTDLE RSTKESEEAV LRDLSWLGPA 

       130        140        150        160        170        180 
WDEGPGIGGE YGPYRQSERN ALYKQFAEKL LQSGHVYRCF CSNEELEKMK EIAKLKQLPP 

       190        200        210        220        230        240 
VYTGRWASAT EEEVVEELAK GTPYTYRFRV PKEGSLKIDD LIRGEVSWNL DTLGDFVIMR 

       250        260        270        280        290        300 
SNGQPVYNFC VTVDDATMAI SHVIRAEEHL PNTLRQALIY KALGFPMPHF AHVSLILAPD 

       310        320        330        340        350        360 
RSKLSKRHGA TSVGQFRDMG YLPQAMVNYL ALLGWGDGTE NEFFTLEQLV EKFTIERVNK 

       370        380        390        400        410        420 
SGAIFDSTKL RWMNGQHLRS LPSEELNRII GERWKDAGIA TESQGIFIQD AVLLLKDGID 

       430        440        450        460        470        480 
LITDSEKALS SLLSYPLYET LASAEGKPIL EDGVSEVAKS LLAAYDSGEL SGALAEGQPG 

       490        500        510        520        530        540 
WQKWAKNFGK LLKRKGKSLF MPLRVLLTGK LHGPDIGATT VLLYKAGTSG SVVPQAGFVT 

       550        560 
FDERFKILRE VQWESFSTDV PLSAGAVTR 

« Hide

References

[1]Andersen R.V.
Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. SR1.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83524 mRNA. Translation: CAA58506.1.
PIRS51685.

3D structure databases

ProteinModelPortalQ43794.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_TOBAC
AccessionPrimary (citable) accession number: Q43794
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries