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Protein

Glutamate--tRNA ligase, chloroplastic/mitochondrial

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69ATPBy similarity1
Binding sitei95GlutamateBy similarity1
Binding sitei265GlutamateBy similarity1
Binding sitei268ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi303 – 307ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase, chloroplastic/mitochondrial (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana
Proteomesi
  • UP000084051 Componenti: Genome assembly

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000119737? – 569Glutamate--tRNA ligase, chloroplastic/mitochondrial
Transit peptidei1 – ?Chloroplast and mitochondrionSequence analysis

Proteomic databases

PRIDEiQ43794

Structurei

3D structure databases

ProteinModelPortaliQ43794
SMRiQ43794
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 61Glutamate bindingBy similarity3
Regioni247 – 251Glutamate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi62 – 72"HIGH" regionAdd BLAST11
Motifi303 – 307"KMSKS" region5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK01885

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43794-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLAAAPWF RVRLIPELKN SQSLLYCRGN HSYRQSLCSR RRSFSVYASA
60 70 80 90 100
GDGGDVRVRF APSPTGNLHV GGARTALFNY LYARAKGGKF ILRIEDTDLE
110 120 130 140 150
RSTKESEEAV LRDLSWLGPA WDEGPGIGGE YGPYRQSERN ALYKQFAEKL
160 170 180 190 200
LQSGHVYRCF CSNEELEKMK EIAKLKQLPP VYTGRWASAT EEEVVEELAK
210 220 230 240 250
GTPYTYRFRV PKEGSLKIDD LIRGEVSWNL DTLGDFVIMR SNGQPVYNFC
260 270 280 290 300
VTVDDATMAI SHVIRAEEHL PNTLRQALIY KALGFPMPHF AHVSLILAPD
310 320 330 340 350
RSKLSKRHGA TSVGQFRDMG YLPQAMVNYL ALLGWGDGTE NEFFTLEQLV
360 370 380 390 400
EKFTIERVNK SGAIFDSTKL RWMNGQHLRS LPSEELNRII GERWKDAGIA
410 420 430 440 450
TESQGIFIQD AVLLLKDGID LITDSEKALS SLLSYPLYET LASAEGKPIL
460 470 480 490 500
EDGVSEVAKS LLAAYDSGEL SGALAEGQPG WQKWAKNFGK LLKRKGKSLF
510 520 530 540 550
MPLRVLLTGK LHGPDIGATT VLLYKAGTSG SVVPQAGFVT FDERFKILRE
560
VQWESFSTDV PLSAGAVTR
Length:569
Mass (Da):63,338
Last modified:November 1, 1996 - v1
Checksum:iF2E81D73460A1844
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83524 mRNA Translation: CAA58506.1
PIRiS51685
RefSeqiNP_001312310.1, NM_001325381.1
UniGeneiNta.4209

Genome annotation databases

GeneIDi107784556
KEGGinta:107784556

Similar proteinsi

Entry informationi

Entry nameiSYE_TOBAC
AccessioniPrimary (citable) accession number: Q43794
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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