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Protein

Glucose-6-phosphate 1-dehydrogenase, chloroplastic

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Enzyme regulationi

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei150 – 1501NADPBy similarity
Binding sitei253 – 2531NADP; via carbonyl oxygenBy similarity
Binding sitei253 – 2531SubstrateBy similarity
Binding sitei321 – 3211SubstrateBy similarity
Binding sitei340 – 3401SubstrateBy similarity
Active sitei345 – 3451Proton acceptorBy similarity
Binding sitei441 – 4411SubstrateBy similarity
Binding sitei446 – 4461SubstrateBy similarity
Binding sitei482 – 4821SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi116 – 1238NADPBy similarity

GO - Molecular functioni

  1. glucose-6-phosphate dehydrogenase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glucose metabolic process Source: UniProtKB-KW
  2. pentose-phosphate shunt Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase, chloroplastic (EC:1.1.1.49)
Short name:
G6PD
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 593Glucose-6-phosphate 1-dehydrogenase, chloroplasticPRO_0000010441
Transit peptidei1 – ?ChloroplastSequence Analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi168 ↔ 176Redox modulationBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ43793.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ43793.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni283 – 2875Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43793-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTLYSSPST HSSGPVASYS NSSIGLYNYH HNKQIAVSSI LSRKFGSLQI
60 70 80 90 100
NQKPFWNAVR MQDGAVATPP SKIENETPLK KLKNGILPVA PPKEQKDTID
110 120 130 140 150
FDSNKAKSTV SITVVGASGD LAKKKIFPAL FALYYEGCLP EHFTIFGYAR
160 170 180 190 200
SKMTDAELRN MVSKTLTCRI DKRENCGEKM EQFLERCFYH SGQYDSLENF
210 220 230 240 250
AELDKKLKEH EAGRFSNRLF YLSIPPNIFI NAVRCASLSA SSAHGWTRVI
260 270 280 290 300
VEKPFGRDSE SSAALTRSLK QYLNEDQIFR IDHYLGKELV ENLSVLRFSN
310 320 330 340 350
LIFEPLWSRQ CIRNVQFIFS EDFGTEGRGG YFDHYGIIRD IMQNHLLQIL
360 370 380 390 400
ALFAMETPVS LDAEDIRNEK VKVLRSMRPL QLDDVIIGQY KCHTKGDVTY
410 420 430 440 450
PGYTDDKTVP KDSLTPTFAA AALFIDNARW DGVPFLMKAG KALHTRSAEI
460 470 480 490 500
RVQFRHVPGN LYNKNFGSDL DQATNELVIR VQPNEAIYLK INNKVPGLGM
510 520 530 540 550
RLDRSNLNLL YSARYSKEIP DAYERLLLDA IEGERRLFIR SDELDAAWSL
560 570 580 590
FTPVLKELED KKIVPEYYPY GSRGPIGAHY LAARYKVRWG DLV
Length:593
Mass (Da):67,369
Last modified:November 1, 1996 - v1
Checksum:i228A7732E510E571
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99405 mRNA. Translation: CAA67782.1.
PIRiT03244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99405 mRNA. Translation: CAA67782.1.
PIRiT03244.

3D structure databases

ProteinModelPortaliQ43793.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ43793.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation of a tobacco chloroplast glucose-6-phosphate dehydrogenase cDNA."
    Knight J.S., Emes M.J.
    Plant Gene Register PGR96-076
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Samsun.
    Tissue: Leaf.

Entry informationi

Entry nameiG6PDC_TOBAC
AccessioniPrimary (citable) accession number: Q43793
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.