Reviewed, UniProtKB/Swiss-Prot Q43793 (G6PDC_TOBAC)

Names and origin

Protein names	Recommended name:     Glucose-6-phosphate 1-dehydrogenase, chloroplastic       Short name=G6PD     EC=1.1.1.49
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division By similarity.
Catalytic activity	D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH.
Enzyme regulation	Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 593		Glucose-6-phosphate 1-dehydrogenase, chloroplastic		PRO_0000010441
Sites
	Active site	345	1	Proton acceptor By similarity
	Binding site	118	1	NADP By similarity
	Binding site	150	1	NADP By similarity
	Binding site	283	1	Substrate By similarity
	Binding site	287	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	168 ↔ 176		Redox modulation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43793-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 228A7732E510E571	FASTA	593	67,369
	10 20 30 40 50 60 MVTLYSSPST HSSGPVASYS NSSIGLYNYH HNKQIAVSSI LSRKFGSLQI NQKPFWNAVR 70 80 90 100 110 120 MQDGAVATPP SKIENETPLK KLKNGILPVA PPKEQKDTID FDSNKAKSTV SITVVGASGD 130 140 150 160 170 180 LAKKKIFPAL FALYYEGCLP EHFTIFGYAR SKMTDAELRN MVSKTLTCRI DKRENCGEKM 190 200 210 220 230 240 EQFLERCFYH SGQYDSLENF AELDKKLKEH EAGRFSNRLF YLSIPPNIFI NAVRCASLSA 250 260 270 280 290 300 SSAHGWTRVI VEKPFGRDSE SSAALTRSLK QYLNEDQIFR IDHYLGKELV ENLSVLRFSN 310 320 330 340 350 360 LIFEPLWSRQ CIRNVQFIFS EDFGTEGRGG YFDHYGIIRD IMQNHLLQIL ALFAMETPVS 370 380 390 400 410 420 LDAEDIRNEK VKVLRSMRPL QLDDVIIGQY KCHTKGDVTY PGYTDDKTVP KDSLTPTFAA 430 440 450 460 470 480 AALFIDNARW DGVPFLMKAG KALHTRSAEI RVQFRHVPGN LYNKNFGSDL DQATNELVIR 490 500 510 520 530 540 VQPNEAIYLK INNKVPGLGM RLDRSNLNLL YSARYSKEIP DAYERLLLDA IEGERRLFIR 550 560 570 580 590 SDELDAAWSL FTPVLKELED KKIVPEYYPY GSRGPIGAHY LAARYKVRWG DLV

References

[1]	"Isolation of a tobacco chloroplast glucose-6-phosphate dehydrogenase cDNA." Knight J.S., Emes M.J. Plant Gene Register PGR96-076 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Samsun. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X99405 mRNA. Translation: CAA67782.1.
PIR	T03244.
3D structure databases
HSSP	HSSP built from PDB template 1QKI based on UniProtKB P11413.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.49. 298.
Family and domain databases
InterPro	IPR001282. Glc-6-P_DH. IPR019796. Glc-6-P_DH_AS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
ProDom	PD001129. G6PD. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PDC_TOBAC

Accession

Primary (citable) accession number: Q43793

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)