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Q43793 (G6PDC_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate 1-dehydrogenase, chloroplastic

Short name=G6PD
EC=1.1.1.49
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length593 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division By similarity. HAMAP-Rule MF_00966

Catalytic activity

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH. HAMAP-Rule MF_00966

Enzyme regulation

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity. HAMAP-Rule MF_00966

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. HAMAP-Rule MF_00966

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00966

Subcellular location

Plastidchloroplast HAMAP-Rule MF_00966.

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandNADP
   Molecular functionOxidoreductase
   PTMDisulfide bond
Gene Ontology (GO)
   Biological_processpentose-phosphate shunt

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADP binding

Inferred from electronic annotation. Source: InterPro

glucose-6-phosphate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 593Glucose-6-phosphate 1-dehydrogenase, chloroplastic HAMAP-Rule MF_00966PRO_0000010441

Regions

Nucleotide binding116 – 1238NADP By similarity
Region283 – 2875Substrate binding By similarity

Sites

Active site3451Proton acceptor By similarity
Binding site1501NADP By similarity
Binding site2531NADP; via carbonyl oxygen By similarity
Binding site2531Substrate By similarity
Binding site3211Substrate By similarity
Binding site3401Substrate By similarity
Binding site4411Substrate By similarity
Binding site4461Substrate By similarity
Binding site4821Substrate By similarity

Amino acid modifications

Disulfide bond168 ↔ 176Redox modulation By similarity

Sequences

Sequence LengthMass (Da)Tools
Q43793 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 228A7732E510E571

FASTA59367,369
        10         20         30         40         50         60 
MVTLYSSPST HSSGPVASYS NSSIGLYNYH HNKQIAVSSI LSRKFGSLQI NQKPFWNAVR 

        70         80         90        100        110        120 
MQDGAVATPP SKIENETPLK KLKNGILPVA PPKEQKDTID FDSNKAKSTV SITVVGASGD 

       130        140        150        160        170        180 
LAKKKIFPAL FALYYEGCLP EHFTIFGYAR SKMTDAELRN MVSKTLTCRI DKRENCGEKM 

       190        200        210        220        230        240 
EQFLERCFYH SGQYDSLENF AELDKKLKEH EAGRFSNRLF YLSIPPNIFI NAVRCASLSA 

       250        260        270        280        290        300 
SSAHGWTRVI VEKPFGRDSE SSAALTRSLK QYLNEDQIFR IDHYLGKELV ENLSVLRFSN 

       310        320        330        340        350        360 
LIFEPLWSRQ CIRNVQFIFS EDFGTEGRGG YFDHYGIIRD IMQNHLLQIL ALFAMETPVS 

       370        380        390        400        410        420 
LDAEDIRNEK VKVLRSMRPL QLDDVIIGQY KCHTKGDVTY PGYTDDKTVP KDSLTPTFAA 

       430        440        450        460        470        480 
AALFIDNARW DGVPFLMKAG KALHTRSAEI RVQFRHVPGN LYNKNFGSDL DQATNELVIR 

       490        500        510        520        530        540 
VQPNEAIYLK INNKVPGLGM RLDRSNLNLL YSARYSKEIP DAYERLLLDA IEGERRLFIR 

       550        560        570        580        590 
SDELDAAWSL FTPVLKELED KKIVPEYYPY GSRGPIGAHY LAARYKVRWG DLV 

« Hide

References

[1]"Isolation of a tobacco chloroplast glucose-6-phosphate dehydrogenase cDNA."
Knight J.S., Emes M.J.
Plant Gene Register PGR96-076
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Samsun.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X99405 mRNA. Translation: CAA67782.1.
PIRT03244.

3D structure databases

ProteinModelPortalQ43793.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ43793.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00115; UER00408.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00966. G6PD.
InterProIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23429. PTHR23429. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFPIRSF000110. G6PD. 1 hit.
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR00871. zwf. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG6PDC_TOBAC
AccessionPrimary (citable) accession number: Q43793
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways