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Q43785

- GLNA3_MEDSA

UniProt

Q43785 - GLNA3_MEDSA

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Protein

Glutamine synthetase nodule isozyme

Gene

GS1

Organism
Medicago sativa (Alfalfa)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase nodule isozyme (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase
Gene namesi
Name:GS1
OrganismiMedicago sativa (Alfalfa)
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Glutamine synthetase nodule isozymePRO_0000153184Add
BLAST

Expressioni

Tissue specificityi

Found at highest levels in root nodules.

Interactioni

Subunit structurei

Homooctamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ43785.
SMRiQ43785. Positions 4-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q43785-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLLSDLINL NLSESSEKII AEYIWVGGSG MDLRSKARTL PGPVSDPAKL
60 70 80 90 100
PKWNYDGSST NQAPGQDSEV ILYPQAIFKD PFRQGNNILV ICDVYTPAGE
110 120 130 140 150
PLPTNKRHNA AKIFSHPDVA AEVPWYGIEQ EYTLLQKDTN WPLGWPIGGF
160 170 180 190 200
PGPQGPYYCG IGADKAYGRD IVDAHYKACL YAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPSVGISA GDEIWAARYI LERITEIAGV VVSFDPKPIP GDWNGAGAHT
260 270 280 290 300
NYSTKSMRED GGYEIIKKAI EKLGLRHKEH IAAYGEGNER RLTGKHETTD
310 320 330 340 350
INTFSWGVAN RGASVRVGRD TEKDGKGYFE DRRPSSNMDP YVVTSMIAET

TLLWKP
Length:356
Mass (Da):39,152
Last modified:November 1, 1996 - v1
Checksum:i5368C4BD10C96ECB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15591 mRNA. Translation: AAB41554.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15591 mRNA. Translation: AAB41554.1 .

3D structure databases

ProteinModelPortali Q43785.
SMRi Q43785. Positions 4-355.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of a nodule-enhanced glutamine synthetase from alfalfa: nucleotide sequence, in situ localization, and transcript analysis."
    Temple S.J., Heard J., Ganter G., Dunn K., Sengupta-Gopalan C.
    Mol. Plant Microbe Interact. 8:218-227(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Iroquois.
    Tissue: Root nodule.
  2. "Developmental regulation of nodule-specific genes in alfalfa root nodules."
    Dunn K., Dickstein R., Feinbaum R., Burnett B.K., Peterman T.K., Thoidis G., Goodman H.M., Ausubel F.M.
    Mol. Plant Microbe Interact. 1:66-74(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE.
    Strain: cv. Iroquois.
    Tissue: Root nodule.

Entry informationi

Entry nameiGLNA3_MEDSA
AccessioniPrimary (citable) accession number: Q43785
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3