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Q43785 (GLNA3_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase nodule isozyme

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase
Gene names
Name:GS1
OrganismMedicago sativa (Alfalfa)
Taxonomic identifier3879 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer By similarity.

Subcellular location

Cytoplasm.

Tissue specificity

Found at highest levels in root nodules.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Biological processNitrogen fixation
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Glutamine synthetase nodule isozyme
PRO_0000153184

Sequences

Sequence LengthMass (Da)Tools
Q43785 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 5368C4BD10C96ECB

FASTA35639,152
        10         20         30         40         50         60 
MSLLSDLINL NLSESSEKII AEYIWVGGSG MDLRSKARTL PGPVSDPAKL PKWNYDGSST 

        70         80         90        100        110        120 
NQAPGQDSEV ILYPQAIFKD PFRQGNNILV ICDVYTPAGE PLPTNKRHNA AKIFSHPDVA 

       130        140        150        160        170        180 
AEVPWYGIEQ EYTLLQKDTN WPLGWPIGGF PGPQGPYYCG IGADKAYGRD IVDAHYKACL 

       190        200        210        220        230        240 
YAGINISGIN GEVMPGQWEF QVGPSVGISA GDEIWAARYI LERITEIAGV VVSFDPKPIP 

       250        260        270        280        290        300 
GDWNGAGAHT NYSTKSMRED GGYEIIKKAI EKLGLRHKEH IAAYGEGNER RLTGKHETTD 

       310        320        330        340        350 
INTFSWGVAN RGASVRVGRD TEKDGKGYFE DRRPSSNMDP YVVTSMIAET TLLWKP 

« Hide

References

[1]"Characterization of a nodule-enhanced glutamine synthetase from alfalfa: nucleotide sequence, in situ localization, and transcript analysis."
Temple S.J., Heard J., Ganter G., Dunn K., Sengupta-Gopalan C.
Mol. Plant Microbe Interact. 8:218-227(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Iroquois.
Tissue: Root nodule.
[2]"Developmental regulation of nodule-specific genes in alfalfa root nodules."
Dunn K., Dickstein R., Feinbaum R., Burnett B.K., Peterman T.K., Thoidis G., Goodman H.M., Ausubel F.M.
Mol. Plant Microbe Interact. 1:66-74(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE.
Strain: cv. Iroquois.
Tissue: Root nodule.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15591 mRNA. Translation: AAB41554.1.

3D structure databases

ProteinModelPortalQ43785.
SMRQ43785. Positions 4-355.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA3_MEDSA
AccessionPrimary (citable) accession number: Q43785
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families