Reviewed, UniProtKB/Swiss-Prot Q43785 (GLNA3_MEDSA)

Names and origin

Protein names

Recommended name:
    Glutamine synthetase nodule isozyme
    EC=6.3.1.2
Alternative name(s):
    Glutamate--ammonia ligase

Gene names

Name:

GS1

Organism

Medicago sativa (Alfalfa)

Taxonomic identifier

3879 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
Subunit structure	Homooctamer By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Found at highest levels in root nodules.
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Biological process	Nitrogen fixation
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Glutamine synthetase nodule isozyme		PRO_0000153184

Sequences

	Sequence		Length	Mass (Da)
	Q43785-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5368C4BD10C96ECB	FASTA	356	39,152
	10 20 30 40 50 60 MSLLSDLINL NLSESSEKII AEYIWVGGSG MDLRSKARTL PGPVSDPAKL PKWNYDGSST 70 80 90 100 110 120 NQAPGQDSEV ILYPQAIFKD PFRQGNNILV ICDVYTPAGE PLPTNKRHNA AKIFSHPDVA 130 140 150 160 170 180 AEVPWYGIEQ EYTLLQKDTN WPLGWPIGGF PGPQGPYYCG IGADKAYGRD IVDAHYKACL 190 200 210 220 230 240 YAGINISGIN GEVMPGQWEF QVGPSVGISA GDEIWAARYI LERITEIAGV VVSFDPKPIP 250 260 270 280 290 300 GDWNGAGAHT NYSTKSMRED GGYEIIKKAI EKLGLRHKEH IAAYGEGNER RLTGKHETTD 310 320 330 340 350 INTFSWGVAN RGASVRVGRD TEKDGKGYFE DRRPSSNMDP YVVTSMIAET TLLWKP

References

[1]	"Characterization of a nodule-enhanced glutamine synthetase from alfalfa: nucleotide sequence, in situ localization, and transcript analysis." Temple S.J., Heard J., Ganter G., Dunn K., Sengupta-Gopalan C. Mol. Plant Microbe Interact. 8:218-227(1995) [PubMed: 7756692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Iroquois. Tissue: Root nodule.
[2]	"Developmental regulation of nodule-specific genes in alfalfa root nodules." Dunn K., Dickstein R., Feinbaum R., Burnett B.K., Peterman T.K., Thoidis G., Goodman H.M., Ausubel F.M. Mol. Plant Microbe Interact. 1:66-74(1988) [PubMed: 2908768] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE. Strain: cv. Iroquois. Tissue: Root nodule.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U15591 mRNA. Translation: AAB41554.1.
3D structure databases
SMR	Q43785. Positions 4-355.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.1.2. 815.
Family and domain databases
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat. IPR008146. Gln_synth_cat. [Graphical view]
Gene3D	G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
ProDom	PD001057. Gln_synt_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNA3_MEDSA

Accession

Primary (citable) accession number: Q43785

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)