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Q43785

- GLNA3_MEDSA

UniProt

Q43785 - GLNA3_MEDSA

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Protein
Glutamine synthetase nodule isozyme
Gene
GS1
Organism
Medicago sativa (Alfalfa)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase nodule isozyme (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase
Gene namesi
Name:GS1
OrganismiMedicago sativa (Alfalfa)
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Glutamine synthetase nodule isozyme
PRO_0000153184Add
BLAST

Expressioni

Tissue specificityi

Found at highest levels in root nodules.

Interactioni

Subunit structurei

Homooctamer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ43785.
SMRiQ43785. Positions 4-355.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q43785-1 [UniParc]FASTAAdd to Basket

« Hide

MSLLSDLINL NLSESSEKII AEYIWVGGSG MDLRSKARTL PGPVSDPAKL    50
PKWNYDGSST NQAPGQDSEV ILYPQAIFKD PFRQGNNILV ICDVYTPAGE 100
PLPTNKRHNA AKIFSHPDVA AEVPWYGIEQ EYTLLQKDTN WPLGWPIGGF 150
PGPQGPYYCG IGADKAYGRD IVDAHYKACL YAGINISGIN GEVMPGQWEF 200
QVGPSVGISA GDEIWAARYI LERITEIAGV VVSFDPKPIP GDWNGAGAHT 250
NYSTKSMRED GGYEIIKKAI EKLGLRHKEH IAAYGEGNER RLTGKHETTD 300
INTFSWGVAN RGASVRVGRD TEKDGKGYFE DRRPSSNMDP YVVTSMIAET 350
TLLWKP 356
Length:356
Mass (Da):39,152
Last modified:November 1, 1996 - v1
Checksum:i5368C4BD10C96ECB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15591 mRNA. Translation: AAB41554.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15591 mRNA. Translation: AAB41554.1 .

3D structure databases

ProteinModelPortali Q43785.
SMRi Q43785. Positions 4-355.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of a nodule-enhanced glutamine synthetase from alfalfa: nucleotide sequence, in situ localization, and transcript analysis."
    Temple S.J., Heard J., Ganter G., Dunn K., Sengupta-Gopalan C.
    Mol. Plant Microbe Interact. 8:218-227(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Iroquois.
    Tissue: Root nodule.
  2. "Developmental regulation of nodule-specific genes in alfalfa root nodules."
    Dunn K., Dickstein R., Feinbaum R., Burnett B.K., Peterman T.K., Thoidis G., Goodman H.M., Ausubel F.M.
    Mol. Plant Microbe Interact. 1:66-74(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE.
    Strain: cv. Iroquois.
    Tissue: Root nodule.

Entry informationi

Entry nameiGLNA3_MEDSA
AccessioniPrimary (citable) accession number: Q43785
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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