Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q43776 (SYK_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysine--tRNA ligase

EC=6.1.1.6
Alternative name(s):
Lysyl-tRNA synthetase
Short name=LysRS
Gene names
Name:LYSRS
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Protein attributes

Sequence length588 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00252

Subcellular location

Cytoplasm HAMAP-Rule MF_00252.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlysyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

lysine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 588588Lysine--tRNA ligase HAMAP-Rule MF_00252
PRO_0000152769

Sequences

Sequence LengthMass (Da)Tools
Q43776 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 66590227238C20CF

FASTA58867,116
        10         20         30         40         50         60 
MDSSVSTEPL SKNALKREKK AKEKEQLEQE KKAAAVAKRQ MEQHNLPEND DLDPTQYLAN 

        70         80         90        100        110        120 
RLRNIESLRE SGINPYPHKF FITMSIPEFI SRYAHLNTGE FPEDIDMSLA GRVISKRASS 

       130        140        150        160        170        180 
SKLYFYELLG GGARVQVLAS ARDSDVDAVQ FSNYQSGVKR GDIIGVRGYP GKSKRGELSI 

       190        200        210        220        230        240 
FAKPFIVLAP CLHMLPRRLT SSIVDETRTQ NFQGITAYDT WTPGDLRNPE SYVLRDQETR 

       250        260        270        280        290        300 
YRQRYLDLMM NPEVRALFRT RARIISYIRS FLDNLEFLEV ETPSMNLTAG GASARPFITH 

       310        320        330        340        350        360 
HNELDTELLI RVSPELYLKK LVVGGFDRVY ELGKHFRNEG MDLTHSPEFT MCELYMAYAD 

       370        380        390        400        410        420 
YNDLMDLTEQ LLSGMVKDLT GSYKIRYHAN GLDNEPIEID FTPPFRKIDM LSELEKVANI 

       430        440        450        460        470        480 
SIPRDLSSES ANKHLVDVCE KFDVKCPPPH TTTRLLDKLV GHFIEVNCIN PTFIINHPEI 

       490        500        510        520        530        540 
MSPLAKSRRS EPGLTERFNL FVNRRELCDA YTELNDPTAQ RERFAEQLKD RQLGDDEAMD 

       550        560        570        580 
LDESFITALE YGLPPTGGLG MGIDRLTMLL TDSQNVKEVI LFPAMRLQ 

« Hide

References

[1]"A root-specific iron-regulated gene of tomato encodes a lysyl-tRNA-synthetase-like protein."
Giritch A., Herbik A., Balzer H.-J., Ganal M., Stephan U.W., Baumlein H.
Eur. J. Biochem. 244:310-317(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Bonner Beste.
Tissue: Root.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X94451 mRNA. Translation: CAA64223.1.
PIRT07085.
RefSeqNP_001234285.1. NM_001247356.1.
UniGeneLes.3641.

3D structure databases

ProteinModelPortalQ43776.
SMRQ43776. Positions 52-588.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID778357.
KEGGsly:778357.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
HAMAPMF_00252. Lys_tRNA_synth_class2.
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF4. PTHR22594:SF4. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSPR00982. TRNASYNTHLYS.
SUPFAMSSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00499. lysS_bact. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYK_SOLLC
AccessionPrimary (citable) accession number: Q43776
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries