Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q43768

- SYE_HORVU

UniProt

Q43768 - SYE_HORVU

Protein

Glutamate--tRNA ligase, chloroplastic/mitochondrial

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei57 – 571ATPBy similarity
    Binding sitei83 – 831GlutamateBy similarity
    Binding sitei253 – 2531GlutamateBy similarity
    Binding sitei256 – 2561ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi291 – 2955ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-tRNA ligase activity Source: UniProtKB-EC
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligase, chloroplastic/mitochondrial (EC:6.1.1.17)
    Alternative name(s):
    Glutamyl-tRNA synthetase
    Short name:
    GluRS
    OrganismiHordeum vulgare (Barley)
    Taxonomic identifieri4513 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

    Organism-specific databases

    GrameneiQ43768.

    Subcellular locationi

    Plastidchloroplast Curated. Mitochondrion Curated

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell
    2. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Mitochondrion, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 560Glutamate--tRNA ligase, chloroplastic/mitochondrialPRO_0000119736
    Transit peptidei1 – ?Chloroplast and mitochondrionSequence Analysis

    Expressioni

    Gene expression databases

    GenevestigatoriQ43768.

    Structurei

    3D structure databases

    ProteinModelPortaliQ43768.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni47 – 493Glutamate bindingBy similarity
    Regioni235 – 2395Glutamate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi50 – 6011"HIGH" regionAdd
    BLAST
    Motifi291 – 2955"KMSKS" region

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q43768-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAASNFMGSS ARLRVGLLPS VTPRLSRRAL ATRASADSGG SGPVRVRFAP    50
    SPTGNLHVGG ARTALFNYLF ARSRGGKFVL RVEDTDLERS TKKSEEAVLT 100
    DLSWLGLDWD EGPDIGGDFG PYRQSERNAL YKEHAQKLME SGAVYRCFCS 150
    NEELEKMKET ANRMKIPPVY MGKWATASDA EVQQELEKGT PYTYRFRVPK 200
    EGSLKINDLI RGEVSWNLNT LGDFVIMRSN GQPVYNFCVT VDDATMRISH 250
    VIRAEEHLPN TLRQALIYKA LGFAMPLFAH VSLILAPDKS KLSKRHGATS 300
    VGQYKEMGYL PQAMVNYLAL LGWGDGTENE FFTIDDLVEK FTIDRVNKSG 350
    AVFDATKLKW MNGQHLRSLP SDLLIKDFED QWRSTGILLE SESGFAKEAA 400
    ELLKEGIDLI TDADAALCKL LSYPLHETLS SDEAKSVVED KLSEVASGLI 450
    SAYDSGELDQ ALAEGHDGWK KWVKSFGKTH KRKGKSLFMP LRVLLTGKLH 500
    GPAMDSTVIL VHKAGTSGAV APQSGFVSLD ERFKILKEVN WESLQKQQES 550
    PVESAVPAAS 560
    Length:560
    Mass (Da):61,862
    Last modified:November 1, 1996 - v1
    Checksum:i2F9F830D3A457B9B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83523 mRNA. Translation: CAA58505.1.
    PIRiS51684.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83523 mRNA. Translation: CAA58505.1 .
    PIRi S51684.

    3D structure databases

    ProteinModelPortali Q43768.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei Q43768.

    Gene expression databases

    Genevestigatori Q43768.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Andersen R.V.
      Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Svalofs Bonus.
      Tissue: Leaf.

    Entry informationi

    Entry nameiSYE_HORVU
    AccessioniPrimary (citable) accession number: Q43768
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3