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Protein

Glutamate--tRNA ligase, chloroplastic/mitochondrial

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571ATPBy similarity
Binding sitei83 – 831GlutamateBy similarity
Binding sitei253 – 2531GlutamateBy similarity
Binding sitei256 – 2561ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi291 – 2955ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase, chloroplastic/mitochondrial (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiQ43768.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 560Glutamate--tRNA ligase, chloroplastic/mitochondrialPRO_0000119736
Transit peptidei1 – ?Chloroplast and mitochondrionSequence Analysis

Expressioni

Gene expression databases

GenevestigatoriQ43768.

Structurei

3D structure databases

ProteinModelPortaliQ43768.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni47 – 493Glutamate bindingBy similarity
Regioni235 – 2395Glutamate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionAdd
BLAST
Motifi291 – 2955"KMSKS" region

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43768-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASNFMGSS ARLRVGLLPS VTPRLSRRAL ATRASADSGG SGPVRVRFAP
60 70 80 90 100
SPTGNLHVGG ARTALFNYLF ARSRGGKFVL RVEDTDLERS TKKSEEAVLT
110 120 130 140 150
DLSWLGLDWD EGPDIGGDFG PYRQSERNAL YKEHAQKLME SGAVYRCFCS
160 170 180 190 200
NEELEKMKET ANRMKIPPVY MGKWATASDA EVQQELEKGT PYTYRFRVPK
210 220 230 240 250
EGSLKINDLI RGEVSWNLNT LGDFVIMRSN GQPVYNFCVT VDDATMRISH
260 270 280 290 300
VIRAEEHLPN TLRQALIYKA LGFAMPLFAH VSLILAPDKS KLSKRHGATS
310 320 330 340 350
VGQYKEMGYL PQAMVNYLAL LGWGDGTENE FFTIDDLVEK FTIDRVNKSG
360 370 380 390 400
AVFDATKLKW MNGQHLRSLP SDLLIKDFED QWRSTGILLE SESGFAKEAA
410 420 430 440 450
ELLKEGIDLI TDADAALCKL LSYPLHETLS SDEAKSVVED KLSEVASGLI
460 470 480 490 500
SAYDSGELDQ ALAEGHDGWK KWVKSFGKTH KRKGKSLFMP LRVLLTGKLH
510 520 530 540 550
GPAMDSTVIL VHKAGTSGAV APQSGFVSLD ERFKILKEVN WESLQKQQES
560
PVESAVPAAS
Length:560
Mass (Da):61,862
Last modified:November 1, 1996 - v1
Checksum:i2F9F830D3A457B9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83523 mRNA. Translation: CAA58505.1.
PIRiS51684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83523 mRNA. Translation: CAA58505.1.
PIRiS51684.

3D structure databases

ProteinModelPortaliQ43768.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiQ43768.

Gene expression databases

GenevestigatoriQ43768.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Andersen R.V.
    Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Svalofs Bonus.
    Tissue: Leaf.

Entry informationi

Entry nameiSYE_HORVU
AccessioniPrimary (citable) accession number: Q43768
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 27, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.