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Q43768 (SYE_HORVU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase, chloroplastic/mitochondrial

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022_B

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022_B

Subcellular location

Plastidchloroplast Potential. Mitochondrion Potential HAMAP-Rule MF_00022_B.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentChloroplast
Mitochondrion
Plastid
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
RNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast and mitochondrion Potential
Chain? – 560Glutamate--tRNA ligase, chloroplastic/mitochondrial HAMAP-Rule MF_00022_BPRO_0000119736

Regions

Nucleotide binding291 – 2955ATP By similarity
Region47 – 493Glutamate binding By similarity
Region235 – 2395Glutamate binding By similarity
Motif50 – 6011"HIGH" region HAMAP-Rule MF_00022_B
Motif291 – 2955"KMSKS" region HAMAP-Rule MF_00022_B

Sites

Binding site571ATP By similarity
Binding site831Glutamate By similarity
Binding site2531Glutamate By similarity
Binding site2561ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q43768 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 2F9F830D3A457B9B

FASTA56061,862
        10         20         30         40         50         60 
MAASNFMGSS ARLRVGLLPS VTPRLSRRAL ATRASADSGG SGPVRVRFAP SPTGNLHVGG 

        70         80         90        100        110        120 
ARTALFNYLF ARSRGGKFVL RVEDTDLERS TKKSEEAVLT DLSWLGLDWD EGPDIGGDFG 

       130        140        150        160        170        180 
PYRQSERNAL YKEHAQKLME SGAVYRCFCS NEELEKMKET ANRMKIPPVY MGKWATASDA 

       190        200        210        220        230        240 
EVQQELEKGT PYTYRFRVPK EGSLKINDLI RGEVSWNLNT LGDFVIMRSN GQPVYNFCVT 

       250        260        270        280        290        300 
VDDATMRISH VIRAEEHLPN TLRQALIYKA LGFAMPLFAH VSLILAPDKS KLSKRHGATS 

       310        320        330        340        350        360 
VGQYKEMGYL PQAMVNYLAL LGWGDGTENE FFTIDDLVEK FTIDRVNKSG AVFDATKLKW 

       370        380        390        400        410        420 
MNGQHLRSLP SDLLIKDFED QWRSTGILLE SESGFAKEAA ELLKEGIDLI TDADAALCKL 

       430        440        450        460        470        480 
LSYPLHETLS SDEAKSVVED KLSEVASGLI SAYDSGELDQ ALAEGHDGWK KWVKSFGKTH 

       490        500        510        520        530        540 
KRKGKSLFMP LRVLLTGKLH GPAMDSTVIL VHKAGTSGAV APQSGFVSLD ERFKILKEVN 

       550        560 
WESLQKQQES PVESAVPAAS 

« Hide

References

[1]Andersen R.V.
Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Svalofs Bonus.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83523 mRNA. Translation: CAA58505.1.
PIRS51684.

3D structure databases

ProteinModelPortalQ43768.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ43768.

Gene expression databases

GenevestigatorQ43768.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_HORVU
AccessionPrimary (citable) accession number: Q43768
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries