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Protein

Alpha-glucosidase

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei437 – 4371By similarity
Active sitei440 – 4401By similarity
Active sitei534 – 5341Proton donorBy similarity

GO - Molecular functioni

  1. alpha-1,4-glucosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. maltose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-glucosidase (EC:3.2.1.20)
Alternative name(s):
Maltase
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiQ43763.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 877854Alpha-glucosidasePRO_0000018583Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi338 – 3381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi391 – 3911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi471 – 4711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi570 – 5701N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

High levels seen in the aleurone and scutellum after germination, while low levels are found in developing seeds.

Developmental stagei

Levels increase steadily throughout imbibition reaching maximum levels at day 7. During germination, levels increase from day 2, reach maximum levels at day 3 and decline after day 5.

Inductioni

By gibberellin A3 (GA).

Gene expression databases

ExpressionAtlasiQ43763. baseline.
GenevestigatoriQ43763.

Structurei

3D structure databases

ProteinModelPortaliQ43763.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43763-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATVGVLLLC LCLCLFAPRL CSSKEEGPLA ARTVLAVAVT MEGALRAEAA
60 70 80 90 100
TGGRSSTGDV QRLAVYASLE TDSRLRVRIT DADHPRWEVP QDIIPRPAPG
110 120 130 140 150
DVLHDAPPAS SAPLQGRVLS PAGSDLVLTV HASPFRFTVS RRSTGDTLFD
160 170 180 190 200
TAPGLVFRDK YLEVTSALPA GRASLYGLGE HTKSSFRLRH NDSFTLWNAD
210 220 230 240 250
IGASYVDVNL YGSHPFYMDV RAPGTAHGVL LLSSNGMDVL YGGSYVTYKV
260 270 280 290 300
IGGVLDFYFF AGPNPLAVVD QYTQLIARPA PMPYWSFGFH QCRYGYLNVS
310 320 330 340 350
DLERVVARYA KARIPLEVMW TDIDYMDGFK DFTLDRVNFT AAELRPFVDR
360 370 380 390 400
LHRNAQKYVL ILDPGIRVDP IDATYGTFVR GMQQDIFLKR NGTNFVGNVW
410 420 430 440 450
PGDVYFPDFM HPAAAEFWAR EISLFRRTIP VDGLWIDMNE ISNFYNPEPM
460 470 480 490 500
NALDDPPYRI NNDGTGRPIN NKTVRPLAVH YGGVTEYEEH NLFGLLEARA
510 520 530 540 550
TGRGVLRDTG RRPFVLSRST FVGSGRYTAY WTGDNAATWG DLRYSINTML
560 570 580 590 600
SFGLFGMPMI GADICGFNGN TTEELCGRWI QLGAFYPFSR DHSAIFTVRR
610 620 630 640 650
ELYLWPSVAA SGRKALGLRY QLLPYFYTLM YEAHMTGAPI ARPLFFSYPH
660 670 680 690 700
DVATYGVDRQ FLLGRGVLVS PVLEPGPTTV DAYFPAGRWY RLYDYSLAVA
710 720 730 740 750
TRTGKHVRLP APADTVNVHL TGGTILPLQQ SALTTSRARR TAFHLLVALA
760 770 780 790 800
EDGTASGYLF LDDGDSPEYG RRSDWSMVRF NYKIPNNKGA IKVKSEVVHN
810 820 830 840 850
SYAQSRTLVI SKVVLMGHRS PAAPKKLTVH VNSAEVEASS SAGTRYQNAG
860 870
GLGGVAHIGG LSLVVGEEFE LKVAMSY
Length:877
Mass (Da):96,933
Last modified:November 1, 1996 - v1
Checksum:iE3B5C16E4588C492
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22450 mRNA. Translation: AAB02985.1.
PIRiS65057.
UniGeneiHv.20758.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22450 mRNA. Translation: AAB02985.1.
PIRiS65057.
UniGeneiHv.20758.

3D structure databases

ProteinModelPortaliQ43763.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiQ43763.

Gene expression databases

ExpressionAtlasiQ43763. baseline.
GenevestigatoriQ43763.

Family and domain databases

InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of a gibberellin-inducible, putative alpha-glucosidase gene from barley."
    Tibbot B.K., Skadsen R.W.
    Plant Mol. Biol. 30:229-241(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Morex.
    Tissue: Aleurone.

Entry informationi

Entry nameiAGLU_HORVU
AccessioniPrimary (citable) accession number: Q43763
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.