Q43763 (AGLU_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alpha-glucosidase EC=3.2.1.20 Alternative name(s): Maltase
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	877 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Tissue specificity	High levels seen in the aleurone and scutellum after germination, while low levels are found in developing seeds.
Developmental stage	Levels increase steadily throughout imbibition reaching maximum levels at day 7. During germination, levels increase from day 2, reach maximum levels at day 3 and decline after day 5.
Induction	By gibberellin A3 (GA).
Sequence similarities	Belongs to the glycosyl hydrolase 31 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro maltose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 877	854	Alpha-glucosidase		PRO_0000018583
Sites
	Active site	437	1	By similarity
	Active site	440	1	By similarity
	Active site	534	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
	Glycosylation	338	1	N-linked (GlcNAc...) Potential
	Glycosylation	391	1	N-linked (GlcNAc...) Potential
	Glycosylation	471	1	N-linked (GlcNAc...) Potential
	Glycosylation	570	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q43763 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E3B5C16E4588C492	FASTA	877	96,933
	10 20 30 40 50 60 MATVGVLLLC LCLCLFAPRL CSSKEEGPLA ARTVLAVAVT MEGALRAEAA TGGRSSTGDV 70 80 90 100 110 120 QRLAVYASLE TDSRLRVRIT DADHPRWEVP QDIIPRPAPG DVLHDAPPAS SAPLQGRVLS 130 140 150 160 170 180 PAGSDLVLTV HASPFRFTVS RRSTGDTLFD TAPGLVFRDK YLEVTSALPA GRASLYGLGE 190 200 210 220 230 240 HTKSSFRLRH NDSFTLWNAD IGASYVDVNL YGSHPFYMDV RAPGTAHGVL LLSSNGMDVL 250 260 270 280 290 300 YGGSYVTYKV IGGVLDFYFF AGPNPLAVVD QYTQLIARPA PMPYWSFGFH QCRYGYLNVS 310 320 330 340 350 360 DLERVVARYA KARIPLEVMW TDIDYMDGFK DFTLDRVNFT AAELRPFVDR LHRNAQKYVL 370 380 390 400 410 420 ILDPGIRVDP IDATYGTFVR GMQQDIFLKR NGTNFVGNVW PGDVYFPDFM HPAAAEFWAR 430 440 450 460 470 480 EISLFRRTIP VDGLWIDMNE ISNFYNPEPM NALDDPPYRI NNDGTGRPIN NKTVRPLAVH 490 500 510 520 530 540 YGGVTEYEEH NLFGLLEARA TGRGVLRDTG RRPFVLSRST FVGSGRYTAY WTGDNAATWG 550 560 570 580 590 600 DLRYSINTML SFGLFGMPMI GADICGFNGN TTEELCGRWI QLGAFYPFSR DHSAIFTVRR 610 620 630 640 650 660 ELYLWPSVAA SGRKALGLRY QLLPYFYTLM YEAHMTGAPI ARPLFFSYPH DVATYGVDRQ 670 680 690 700 710 720 FLLGRGVLVS PVLEPGPTTV DAYFPAGRWY RLYDYSLAVA TRTGKHVRLP APADTVNVHL 730 740 750 760 770 780 TGGTILPLQQ SALTTSRARR TAFHLLVALA EDGTASGYLF LDDGDSPEYG RRSDWSMVRF 790 800 810 820 830 840 NYKIPNNKGA IKVKSEVVHN SYAQSRTLVI SKVVLMGHRS PAAPKKLTVH VNSAEVEASS 850 860 870 SAGTRYQNAG GLGGVAHIGG LSLVVGEEFE LKVAMSY

References

[1]	"Molecular cloning and characterization of a gibberellin-inducible, putative alpha-glucosidase gene from barley." Tibbot B.K., Skadsen R.W. Plant Mol. Biol. 30:229-241(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Morex. Tissue: Aleurone.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22450 mRNA. Translation: AAB02985.1.
PIR	S65057.
UniGene	Hv.20758.
3D structure databases
ProteinModelPortal	Q43763.
ModBase	Search...
Protein family/group databases
CAZy	GH31. Glycoside Hydrolase Family 31.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q43763.
Gene expression databases
Genevestigator	Q43763.
Family and domain databases
InterPro	IPR011013. Gal_mutarotase_SF_dom. IPR000322. Glyco_hydro_31. IPR025887. Glyco_hydro_31_N_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR22762. PTHR22762. 1 hit.
Pfam	PF13802. Gal_mutarotas_2. 1 hit. PF01055. Glyco_hydro_31. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit. PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGLU_HORVU

Accession

Primary (citable) accession number: Q43763

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program