Q43754 (CFI_DIACA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chalcone--flavonone isomerase
Short name=Chalcone isomerase
EC=5.5.1.6

Gene names

Name:

CHI

Organism

Dianthus caryophyllus (Carnation) (Clove pink)

Taxonomic identifier

3570 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Dianthus

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin By similarity.
Catalytic activity	A chalcone = a flavanone.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Miscellaneous	Part of the biosynthetic pathway for all classes of flavonoids, a large class of secondary plant metabolites, many of which are brightly colored.
Sequence similarities	Belongs to the chalcone isomerase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	cellular modified amino acid biosynthetic process Inferred from electronic annotation. Source: InterPro flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chalcone isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Chalcone--flavonone isomerase		PRO_0000166430
Sites
	Binding site	47	1	Substrate By similarity
	Binding site	112	1	Substrate By similarity
	Binding site	189	1	Substrate By similarity
	Site	105	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43754 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 184FDD5496D03888	FASTA	221	24,088
	10 20 30 40 50 60 MAEITQIQVE SHVFPPEVKP PGSDKTFFLG GAGVRGLEIQ GKFIKFTAIA VYIEDDAVSS 70 80 90 100 110 120 LAVKWKGKTA DELTDSVDFF KDIVTGPFEK FTQVTMILPL TGQQYSEKVV ENCVAHWKSV 130 140 150 160 170 180 GTYTDAESEA TQKFLQVFKN VSNPPGASIL FTQSPLGSLT ISFSKDGSLP ENGKEVIVNK 190 200 210 220 QLSEAVLQSI IGKHGVSPEA KKSLSARLSD LINQHENPKK E

References

[1]	Henkel J., Ruhnau-Brich B., Dedio J., Wassenegger M., Sommer H., Forkmann G. Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Tanga. Tissue: Petal.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z67989 mRNA. Translation: CAA91931.1.
PIR	T10712.
3D structure databases
ProteinModelPortal	Q43754.
SMR	Q43754. Positions 4-214.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016087. Chalcone_isomerase. IPR016088. Chalcone_isomerase_3-sand. IPR003466. Chalcone_isomerase_subgr. [Graphical view]
Gene3D	G3DSA:3.50.70.10. Chalcone. 1 hit.
Pfam	PF02431. Chalcone. 1 hit. [Graphical view]
SUPFAM	SSF54626. Chalcone. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CFI_DIACA

Accession

Primary (citable) accession number: Q43754

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 31, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program