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Q43744

- MDHM_BRANA

UniProt

Q43744 - MDHM_BRANA

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Protein

Malate dehydrogenase, mitochondrial

Gene

MDH

Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621NADBy similarity
Binding sitei109 – 1091SubstrateBy similarity
Binding sitei115 – 1151SubstrateBy similarity
Binding sitei122 – 1221NADBy similarity
Binding sitei147 – 1471SubstrateBy similarity
Binding sitei181 – 1811SubstrateBy similarity
Binding sitei256 – 2561NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 427NADBy similarity
Nucleotide bindingi145 – 1473NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, mitochondrial (EC:1.1.1.37)
Gene namesi
Name:MDH
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2222MitochondrionSequence AnalysisAdd
BLAST
Chaini23 – 341319Malate dehydrogenase, mitochondrialPRO_0000018623Add
BLAST

Proteomic databases

PRIDEiQ43744.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ43744.
SMRiQ43744. Positions 31-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43744 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFRSALVRSS ASAKQSLLRR SFSSGSVPER KVAILGAAGG IGQPLALLMK
60 70 80 90 100
LNPLVSSLSL YDIANTPGVA ADVGHINTRS QVVGYMGDDN LAKALEGADL
110 120 130 140 150
VIIPAGVPRK PGMTRDDLFN INAGIVKNLW SAIAKYCPHA LVNMISNPVN
160 170 180 190 200
STVPIAAEIF KKAGMYDEKK LFGVTTLDVV RVKTSYAGKA NVPVAEVNVP
210 220 230 240 250
AIVGHAGVTI LPLFSQATPQ AILSGDALTV TTKRTQDGGT EVEEAKAGKG
260 270 280 290 300
SATLSMAYAG ALFADACLKG LNGVPDVVEC SYVQSTITEL PFFASKVRLG
310 320 330 340
KNGVEEVLDL GPLSDFEKEG LEALRPGIKS TIEKGVKFAN Q
Length:341
Mass (Da):35,711
Last modified:November 1, 1997 - v1
Checksum:iF27AC7D2680601C6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89451 mRNA. Translation: CAA61621.1.
PIRiS57958.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89451 mRNA. Translation: CAA61621.1 .
PIRi S57958.

3D structure databases

ProteinModelPortali Q43744.
SMRi Q43744. Positions 31-338.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q43744.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A full-length cDNA coding for mitochondrial malate dehydrogenase from Brassica napus L."
    Witt U., Gruneberg-Seiler M., Abel W.O.
    Plant Gene Register PGR95-068
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Andor.
    Tissue: Leaf.

Entry informationi

Entry nameiMDHM_BRANA
AccessioniPrimary (citable) accession number: Q43744
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3