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Q43744 (MDHM_BRANA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase, mitochondrial

EC=1.1.1.37
Gene names
Name:MDH
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion matrix.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2222Mitochondrion Potential
Chain23 – 341319Malate dehydrogenase, mitochondrial
PRO_0000018623

Regions

Nucleotide binding36 – 427NAD By similarity
Nucleotide binding145 – 1473NAD By similarity

Sites

Binding site621NAD By similarity
Binding site1091Substrate By similarity
Binding site1151Substrate By similarity
Binding site1221NAD By similarity
Binding site1471Substrate By similarity
Binding site1811Substrate By similarity
Binding site2561NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q43744 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: F27AC7D2680601C6

FASTA34135,711
        10         20         30         40         50         60 
MFRSALVRSS ASAKQSLLRR SFSSGSVPER KVAILGAAGG IGQPLALLMK LNPLVSSLSL 

        70         80         90        100        110        120 
YDIANTPGVA ADVGHINTRS QVVGYMGDDN LAKALEGADL VIIPAGVPRK PGMTRDDLFN 

       130        140        150        160        170        180 
INAGIVKNLW SAIAKYCPHA LVNMISNPVN STVPIAAEIF KKAGMYDEKK LFGVTTLDVV 

       190        200        210        220        230        240 
RVKTSYAGKA NVPVAEVNVP AIVGHAGVTI LPLFSQATPQ AILSGDALTV TTKRTQDGGT 

       250        260        270        280        290        300 
EVEEAKAGKG SATLSMAYAG ALFADACLKG LNGVPDVVEC SYVQSTITEL PFFASKVRLG 

       310        320        330        340 
KNGVEEVLDL GPLSDFEKEG LEALRPGIKS TIEKGVKFAN Q 

« Hide

References

[1]"A full-length cDNA coding for mitochondrial malate dehydrogenase from Brassica napus L."
Witt U., Gruneberg-Seiler M., Abel W.O.
Plant Gene Register PGR95-068
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Andor.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89451 mRNA. Translation: CAA61621.1.
PIRS57958.

3D structure databases

ProteinModelPortalQ43744.
SMRQ43744. Positions 31-338.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ43744.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PTHR11540:SF1. PTHR11540:SF1. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDHM_BRANA
AccessionPrimary (citable) accession number: Q43744
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families