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Q43744

- MDHM_BRANA

UniProt

Q43744 - MDHM_BRANA

Protein

Malate dehydrogenase, mitochondrial

Gene

MDH

Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei62 – 621NADBy similarity
    Binding sitei109 – 1091SubstrateBy similarity
    Binding sitei115 – 1151SubstrateBy similarity
    Binding sitei122 – 1221NADBy similarity
    Binding sitei147 – 1471SubstrateBy similarity
    Binding sitei181 – 1811SubstrateBy similarity
    Binding sitei256 – 2561NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi36 – 427NADBy similarity
    Nucleotide bindingi145 – 1473NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase, mitochondrial (EC:1.1.1.37)
    Gene namesi
    Name:MDH
    OrganismiBrassica napus (Rape)
    Taxonomic identifieri3708 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2222MitochondrionSequence AnalysisAdd
    BLAST
    Chaini23 – 341319Malate dehydrogenase, mitochondrialPRO_0000018623Add
    BLAST

    Proteomic databases

    PRIDEiQ43744.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ43744.
    SMRiQ43744. Positions 31-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q43744-1 [UniParc]FASTAAdd to Basket

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    MFRSALVRSS ASAKQSLLRR SFSSGSVPER KVAILGAAGG IGQPLALLMK    50
    LNPLVSSLSL YDIANTPGVA ADVGHINTRS QVVGYMGDDN LAKALEGADL 100
    VIIPAGVPRK PGMTRDDLFN INAGIVKNLW SAIAKYCPHA LVNMISNPVN 150
    STVPIAAEIF KKAGMYDEKK LFGVTTLDVV RVKTSYAGKA NVPVAEVNVP 200
    AIVGHAGVTI LPLFSQATPQ AILSGDALTV TTKRTQDGGT EVEEAKAGKG 250
    SATLSMAYAG ALFADACLKG LNGVPDVVEC SYVQSTITEL PFFASKVRLG 300
    KNGVEEVLDL GPLSDFEKEG LEALRPGIKS TIEKGVKFAN Q 341
    Length:341
    Mass (Da):35,711
    Last modified:November 1, 1997 - v1
    Checksum:iF27AC7D2680601C6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89451 mRNA. Translation: CAA61621.1.
    PIRiS57958.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89451 mRNA. Translation: CAA61621.1 .
    PIRi S57958.

    3D structure databases

    ProteinModelPortali Q43744.
    SMRi Q43744. Positions 31-338.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q43744.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A full-length cDNA coding for mitochondrial malate dehydrogenase from Brassica napus L."
      Witt U., Gruneberg-Seiler M., Abel W.O.
      Plant Gene Register PGR95-068
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Andor.
      Tissue: Leaf.

    Entry informationi

    Entry nameiMDHM_BRANA
    AccessioniPrimary (citable) accession number: Q43744
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3