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Q43743

- MDHG1_BRANA

UniProt

Q43743 - MDHG1_BRANA

Protein

Malate dehydrogenase 1, glyoxysomal

Gene

MDH1

Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei79 – 791NADBy similarity
    Binding sitei126 – 1261SubstratePROSITE-ProRule annotation
    Binding sitei132 – 1321SubstratePROSITE-ProRule annotation
    Binding sitei139 – 1391NADBy similarity
    Binding sitei164 – 1641SubstratePROSITE-ProRule annotation
    Binding sitei198 – 1981SubstratePROSITE-ProRule annotation
    Active sitei222 – 2221Proton acceptorBy similarity
    Binding sitei273 – 2731NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi53 – 597NADBy similarity
    Nucleotide bindingi162 – 1643NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-KW
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase 1, glyoxysomal (EC:1.1.1.37)
    Gene namesi
    Name:MDH1
    OrganismiBrassica napus (Rape)
    Taxonomic identifieri3708 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

    Subcellular locationi

    GO - Cellular componenti

    1. glyoxysome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3838GlyoxysomeSequence AnalysisAdd
    BLAST
    Chaini39 – 358320Malate dehydrogenase 1, glyoxysomalPRO_0000018636Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ43743.
    SMRiQ43743. Positions 46-357.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q43743-1 [UniParc]FASTAAdd to Basket

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    MEFRGDAYKR IAMISAHLQP SFTPQMEAKN SVMGLESCRA KGGNPGFKVA    50
    ILGAAGGIGQ SLSLLMKMNP LVSLLHLYDV VNAPGVTADV SHMDTGAVVR 100
    GFLGAKQLED ALTGMDLVII PAGVPRKPGM TRDDLFKINA GIVRTLCEGV 150
    AKCCPNAIVN LISNPVNSTV AIAAEVFKKA GTYDPKKLLG VTTLDVARAN 200
    TFVAEVLGLD PREVDVPVVG GHAGVTILPL LSQVKPPSSF TPSEIEYLTN 250
    RIQNGGTEVV EAKAGAGSAT LSMAYAAAKF ADACLRGLRG DANVIECSFV 300
    ASQVTELAFF ATKVRLGRTG AEEVFQLGPL NEYERVGLEK AKEELAGSIQ 350
    KGVDFIRK 358
    Length:358
    Mass (Da):37,732
    Last modified:December 1, 2000 - v2
    Checksum:iDC94CBC76ACEEE4A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 88MEFRGDAY → MPH1 PublicationCurated
    Sequence conflicti68 – 681M → V in CAA63268. 1 PublicationCurated
    Sequence conflicti72 – 721V → G in CAA63268. 1 PublicationCurated
    Sequence conflicti151 – 1522AK → GG in CAA63268. 1 PublicationCurated
    Sequence conflicti231 – 2311L → S in CAA63268. 1 PublicationCurated
    Sequence conflicti235 – 2351K → T in CAA63268. 1 PublicationCurated
    Sequence conflicti251 – 2511R → K in CAA63268. 1 PublicationCurated
    Sequence conflicti254 – 2541N → H in CAA63268. 1 PublicationCurated
    Sequence conflicti258 – 2581E → D in CAA63268. 1 PublicationCurated
    Sequence conflicti263 – 2631K → H in CAA63268. 1 PublicationCurated
    Sequence conflicti266 – 2661A → V in CAA63268. 1 PublicationCurated
    Sequence conflicti269 – 27911ATLSMAYAAAK → SPLPIILAAP in CAA63268. 1 PublicationCuratedAdd
    BLAST
    Sequence conflicti306 – 3116ELAFFA → DYFLC in CAA63268. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92512 mRNA. Translation: CAA63268.1.
    AJ242712 Genomic DNA. Translation: CAB43994.1.
    PIRiT08015.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92512 mRNA. Translation: CAA63268.1 .
    AJ242712 Genomic DNA. Translation: CAB43994.1 .
    PIRi T08015.

    3D structure databases

    ProteinModelPortali Q43743.
    SMRi Q43743. Positions 46-357.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A full-length cDNA coding for glyoxysomal malate dehydrogenase from Brassica napus L."
      Witt U., Rehberg S., Abel W.O.
      Plant Gene Register PGR95-124
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: cv. Andor.
      Tissue: Leaf.
    2. "Two genes encoding microbody malate dehydrogenase from Brassica napus."
      Imhoff U., Voetz M., Wingender R., Schnabl H., Wolf N.
      Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.

    Entry informationi

    Entry nameiMDHG1_BRANA
    AccessioniPrimary (citable) accession number: Q43743
    Secondary accession number(s): Q9T0L8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3