Reviewed,
UniProtKB/Swiss-Prot Q43743 (MDHG1_BRANA)
Last modified
November 25, 2008.
Version 60.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Malate dehydrogenase 1, glyoxysomal EC=1.1.1.37 | ||
| Gene names |
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| Organism | Brassica napus (Rape) | ||
| Taxonomic identifier | 3708 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica |
Protein attributes
| Sequence length | 358 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | (S)-malate + NAD(+) = oxaloacetate + NADH. |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | |
| Sequence similarities | Belongs to the LDH/MDH superfamily. MDH type 1 family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Glyoxylate bypass Tricarboxylic acid cycle |
| Cellular component | Glyoxysome |
| Domain | Transit peptide |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: InterPro glyoxylate cycleInferred from electronic annotation. Source: UniProtKB-KW malate metabolic processInferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycleInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | glyoxysome Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | L-malate dehydrogenase activity Inferred from electronic annotation. Source: InterPro bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 38 | 38 | Glyoxysome Potential | ||||||
| Chain | 39 – 358 | 320 | Malate dehydrogenase 1, glyoxysomal | PRO_0000018636 | |||||
Regions | |||||||||
| Nucleotide binding | 53 – 59 | 7 | NAD By similarity | ||||||
| Nucleotide binding | 162 – 164 | 3 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 222 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 79 | 1 | NAD By similarity | ||||||
| Binding site | 126 | 1 | Substrate By similarity | ||||||
| Binding site | 132 | 1 | Substrate By similarity | ||||||
| Binding site | 139 | 1 | NAD By similarity | ||||||
| Binding site | 164 | 1 | Substrate By similarity | ||||||
| Binding site | 198 | 1 | Substrate By similarity | ||||||
| Binding site | 273 | 1 | NAD By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 1 – 8 | 8 | MEFRGDAY → MPH Ref.1 | ||||||
| Sequence conflict | 68 | 1 | M → V in CAA63268. Ref.1 | ||||||
| Sequence conflict | 72 | 1 | V → G in CAA63268. Ref.1 | ||||||
| Sequence conflict | 151 – 152 | 2 | AK → GG in CAA63268. Ref.1 | ||||||
| Sequence conflict | 231 | 1 | L → S in CAA63268. Ref.1 | ||||||
| Sequence conflict | 235 | 1 | K → T in CAA63268. Ref.1 | ||||||
| Sequence conflict | 251 | 1 | R → K in CAA63268. Ref.1 | ||||||
| Sequence conflict | 254 | 1 | N → H in CAA63268. Ref.1 | ||||||
| Sequence conflict | 258 | 1 | E → D in CAA63268. Ref.1 | ||||||
| Sequence conflict | 263 | 1 | K → H in CAA63268. Ref.1 | ||||||
| Sequence conflict | 266 | 1 | A → V in CAA63268. Ref.1 | ||||||
| Sequence conflict | 269 – 279 | 11 | ATLSMAYAAAK → SPLPIILAAP in CAA63268. Ref.1 | ||||||
| Sequence conflict | 306 – 311 | 6 | ELAFFA → DYFLC in CAA63268. Ref.1 | ||||||
Sequences
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References
| [1] | "A full-length cDNA coding for glyoxysomal malate dehydrogenase from Brassica napus L." Witt U., Rehberg S., Abel W.O. (er) Plant Gene Register PGR95-124 Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Andor. Tissue: Leaf. |
| [2] | "Two genes encoding microbody malate dehydrogenase from Brassica napus." Imhoff U., Voetz M., Wingender R., Schnabl H., Wolf N. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. |
Cross-references
Sequence databases | |
|---|---|
| X92512 mRNA. Translation: CAA63268.1. AJ242712 Genomic DNA. Translation: CAB43994.1. | |
| PIR | T08015. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1MLD based on UniProtKB P00346. |
| SMR | Q43743. Positions 46-357. |
| ModBase | Search... |
Family and domain databases | |
| InterPro | IPR001557. L-lactate/malate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR001252. Malate_DHase_AS. IPR010097. Malate_DHase_NAD-dep_euk_g_bac. IPR016040. NAD(P)-bd. [Graphical view] |
| Gene3D | G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR11540:SF1. MDH_euk_g_bac. 1 hit. |
| Pfam | PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000102. Lac_mal_DH. 1 hit. |
| TIGRFAMs | TIGR01772. MDH_euk_gproteo. 1 hit. |
| PROSITE | PS00068. MDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MDHG1_BRANA | ||||||||
| Accession | Primary (citable) accession number: Q43743 Secondary accession number(s): Q9T0L8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||

Clusters with


