Reviewed, UniProtKB/Swiss-Prot Q43743 (MDHG1_BRANA)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase 1, glyoxysomal
    EC=1.1.1.37

Gene names

Name:

MDH1

Organism

Brassica napus (Rape)

Taxonomic identifier

3708 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Glyoxysome.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: InterPro binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 38	38	Glyoxysome Potential
	Chain	39 – 358	320	Malate dehydrogenase 1, glyoxysomal		PRO_0000018636
Regions
	Nucleotide binding	53 – 59	7	NAD By similarity
	Nucleotide binding	162 – 164	3	NAD By similarity
Sites
	Active site	222	1	Proton acceptor By similarity
	Binding site	79	1	NAD By similarity
	Binding site	126	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	139	1	NAD By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	198	1	Substrate By similarity
	Binding site	273	1	NAD By similarity
Experimental info
	Sequence conflict	1 – 8	8	MEFRGDAY → MPH Ref.1
	Sequence conflict	68	1	M → V in CAA63268. Ref.1
	Sequence conflict	72	1	V → G in CAA63268. Ref.1
	Sequence conflict	151 – 152	2	AK → GG in CAA63268. Ref.1
	Sequence conflict	231	1	L → S in CAA63268. Ref.1
	Sequence conflict	235	1	K → T in CAA63268. Ref.1
	Sequence conflict	251	1	R → K in CAA63268. Ref.1
	Sequence conflict	254	1	N → H in CAA63268. Ref.1
	Sequence conflict	258	1	E → D in CAA63268. Ref.1
	Sequence conflict	263	1	K → H in CAA63268. Ref.1
	Sequence conflict	266	1	A → V in CAA63268. Ref.1
	Sequence conflict	269 – 279	11	ATLSMAYAAAK → SPLPIILAAP in CAA63268. Ref.1
	Sequence conflict	306 – 311	6	ELAFFA → DYFLC in CAA63268. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q43743-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: DC94CBC76ACEEE4A	FASTA	358	37,732
	10 20 30 40 50 60 MEFRGDAYKR IAMISAHLQP SFTPQMEAKN SVMGLESCRA KGGNPGFKVA ILGAAGGIGQ 70 80 90 100 110 120 SLSLLMKMNP LVSLLHLYDV VNAPGVTADV SHMDTGAVVR GFLGAKQLED ALTGMDLVII 130 140 150 160 170 180 PAGVPRKPGM TRDDLFKINA GIVRTLCEGV AKCCPNAIVN LISNPVNSTV AIAAEVFKKA 190 200 210 220 230 240 GTYDPKKLLG VTTLDVARAN TFVAEVLGLD PREVDVPVVG GHAGVTILPL LSQVKPPSSF 250 260 270 280 290 300 TPSEIEYLTN RIQNGGTEVV EAKAGAGSAT LSMAYAAAKF ADACLRGLRG DANVIECSFV 310 320 330 340 350 ASQVTELAFF ATKVRLGRTG AEEVFQLGPL NEYERVGLEK AKEELAGSIQ KGVDFIRK

References

[1]	"A full-length cDNA coding for glyoxysomal malate dehydrogenase from Brassica napus L." Witt U., Rehberg S., Abel W.O. (er) Plant Gene Register PGR95-124 Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Andor. Tissue: Leaf.
[2]	"Two genes encoding microbody malate dehydrogenase from Brassica napus." Imhoff U., Voetz M., Wingender R., Schnabl H., Wolf N. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X92512 mRNA. Translation: CAA63268.1. AJ242712 Genomic DNA. Translation: CAB43994.1.
PIR	T08015.
3D structure databases
HSSP	HSSP built from PDB template 1MLD based on UniProtKB P00346.
SMR	Q43743. Positions 46-357.
ModBase	Search...
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR001252. Malate_DHase_AS. IPR010097. Malate_DHase_NAD-dep_euk_g_bac. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF1. MDH_euk_g_bac. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDHG1_BRANA

Accession

Primary (citable) accession number: Q43743
Secondary accession number(s): Q9T0L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)