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Q43743

- MDHG1_BRANA

UniProt

Q43743 - MDHG1_BRANA

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Protein

Malate dehydrogenase 1, glyoxysomal

Gene
MDH1
Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791NAD By similarity
Binding sitei126 – 1261Substrate By similarity
Binding sitei132 – 1321Substrate By similarity
Binding sitei139 – 1391NAD By similarity
Binding sitei164 – 1641Substrate By similarity
Binding sitei198 – 1981Substrate By similarity
Active sitei222 – 2221Proton acceptor By similarity
Binding sitei273 – 2731NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi53 – 597NAD By similarity
Nucleotide bindingi162 – 1643NAD By similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase 1, glyoxysomal (EC:1.1.1.37)
Gene namesi
Name:MDH1
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3838Glyoxysome Reviewed predictionAdd
BLAST
Chaini39 – 358320Malate dehydrogenase 1, glyoxysomalPRO_0000018636Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ43743.
SMRiQ43743. Positions 46-357.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43743-1 [UniParc]FASTAAdd to Basket

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MEFRGDAYKR IAMISAHLQP SFTPQMEAKN SVMGLESCRA KGGNPGFKVA    50
ILGAAGGIGQ SLSLLMKMNP LVSLLHLYDV VNAPGVTADV SHMDTGAVVR 100
GFLGAKQLED ALTGMDLVII PAGVPRKPGM TRDDLFKINA GIVRTLCEGV 150
AKCCPNAIVN LISNPVNSTV AIAAEVFKKA GTYDPKKLLG VTTLDVARAN 200
TFVAEVLGLD PREVDVPVVG GHAGVTILPL LSQVKPPSSF TPSEIEYLTN 250
RIQNGGTEVV EAKAGAGSAT LSMAYAAAKF ADACLRGLRG DANVIECSFV 300
ASQVTELAFF ATKVRLGRTG AEEVFQLGPL NEYERVGLEK AKEELAGSIQ 350
KGVDFIRK 358
Length:358
Mass (Da):37,732
Last modified:December 1, 2000 - v2
Checksum:iDC94CBC76ACEEE4A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 88MEFRGDAY → MPH1 Publication
Sequence conflicti68 – 681M → V in CAA63268. 1 Publication
Sequence conflicti72 – 721V → G in CAA63268. 1 Publication
Sequence conflicti151 – 1522AK → GG in CAA63268. 1 Publication
Sequence conflicti231 – 2311L → S in CAA63268. 1 Publication
Sequence conflicti235 – 2351K → T in CAA63268. 1 Publication
Sequence conflicti251 – 2511R → K in CAA63268. 1 Publication
Sequence conflicti254 – 2541N → H in CAA63268. 1 Publication
Sequence conflicti258 – 2581E → D in CAA63268. 1 Publication
Sequence conflicti263 – 2631K → H in CAA63268. 1 Publication
Sequence conflicti266 – 2661A → V in CAA63268. 1 Publication
Sequence conflicti269 – 27911ATLSMAYAAAK → SPLPIILAAP in CAA63268. 1 PublicationAdd
BLAST
Sequence conflicti306 – 3116ELAFFA → DYFLC in CAA63268. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X92512 mRNA. Translation: CAA63268.1.
AJ242712 Genomic DNA. Translation: CAB43994.1.
PIRiT08015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X92512 mRNA. Translation: CAA63268.1 .
AJ242712 Genomic DNA. Translation: CAB43994.1 .
PIRi T08015.

3D structure databases

ProteinModelPortali Q43743.
SMRi Q43743. Positions 46-357.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A full-length cDNA coding for glyoxysomal malate dehydrogenase from Brassica napus L."
    Witt U., Rehberg S., Abel W.O.
    Plant Gene Register PGR95-124
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: cv. Andor.
    Tissue: Leaf.
  2. "Two genes encoding microbody malate dehydrogenase from Brassica napus."
    Imhoff U., Voetz M., Wingender R., Schnabl H., Wolf N.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiMDHG1_BRANA
AccessioniPrimary (citable) accession number: Q43743
Secondary accession number(s): Q9T0L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 1, 2000
Last modified: June 11, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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