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Protein

Malate dehydrogenase 1, glyoxysomal

Gene

MDH1

Organism
Brassica napus (Rape)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791NADBy similarity
Binding sitei126 – 1261SubstratePROSITE-ProRule annotation
Binding sitei132 – 1321SubstratePROSITE-ProRule annotation
Binding sitei139 – 1391NADBy similarity
Binding sitei164 – 1641SubstratePROSITE-ProRule annotation
Binding sitei198 – 1981SubstratePROSITE-ProRule annotation
Active sitei222 – 2221Proton acceptorBy similarity
Binding sitei273 – 2731NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi53 – 597NADBy similarity
Nucleotide bindingi162 – 1643NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase 1, glyoxysomal (EC:1.1.1.37)
Gene namesi
Name:MDH1
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3838GlyoxysomeSequence AnalysisAdd
BLAST
Chaini39 – 358320Malate dehydrogenase 1, glyoxysomalPRO_0000018636Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ43743.
SMRiQ43743. Positions 46-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43743-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEFRGDAYKR IAMISAHLQP SFTPQMEAKN SVMGLESCRA KGGNPGFKVA
60 70 80 90 100
ILGAAGGIGQ SLSLLMKMNP LVSLLHLYDV VNAPGVTADV SHMDTGAVVR
110 120 130 140 150
GFLGAKQLED ALTGMDLVII PAGVPRKPGM TRDDLFKINA GIVRTLCEGV
160 170 180 190 200
AKCCPNAIVN LISNPVNSTV AIAAEVFKKA GTYDPKKLLG VTTLDVARAN
210 220 230 240 250
TFVAEVLGLD PREVDVPVVG GHAGVTILPL LSQVKPPSSF TPSEIEYLTN
260 270 280 290 300
RIQNGGTEVV EAKAGAGSAT LSMAYAAAKF ADACLRGLRG DANVIECSFV
310 320 330 340 350
ASQVTELAFF ATKVRLGRTG AEEVFQLGPL NEYERVGLEK AKEELAGSIQ

KGVDFIRK
Length:358
Mass (Da):37,732
Last modified:December 1, 2000 - v2
Checksum:iDC94CBC76ACEEE4A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 88MEFRGDAY → MPH1 PublicationCurated
Sequence conflicti68 – 681M → V in CAA63268. 1 PublicationCurated
Sequence conflicti72 – 721V → G in CAA63268. 1 PublicationCurated
Sequence conflicti151 – 1522AK → GG in CAA63268. 1 PublicationCurated
Sequence conflicti231 – 2311L → S in CAA63268. 1 PublicationCurated
Sequence conflicti235 – 2351K → T in CAA63268. 1 PublicationCurated
Sequence conflicti251 – 2511R → K in CAA63268. 1 PublicationCurated
Sequence conflicti254 – 2541N → H in CAA63268. 1 PublicationCurated
Sequence conflicti258 – 2581E → D in CAA63268. 1 PublicationCurated
Sequence conflicti263 – 2631K → H in CAA63268. 1 PublicationCurated
Sequence conflicti266 – 2661A → V in CAA63268. 1 PublicationCurated
Sequence conflicti269 – 27911ATLSMAYAAAK → SPLPIILAAP in CAA63268. 1 PublicationCuratedAdd
BLAST
Sequence conflicti306 – 3116ELAFFA → DYFLC in CAA63268. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92512 mRNA. Translation: CAA63268.1.
AJ242712 Genomic DNA. Translation: CAB43994.1.
PIRiT08015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92512 mRNA. Translation: CAA63268.1.
AJ242712 Genomic DNA. Translation: CAB43994.1.
PIRiT08015.

3D structure databases

ProteinModelPortaliQ43743.
SMRiQ43743. Positions 46-357.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A full-length cDNA coding for glyoxysomal malate dehydrogenase from Brassica napus L."
    Witt U., Rehberg S., Abel W.O.
    Plant Gene Register PGR95-124
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: cv. Andor.
    Tissue: Leaf.
  2. "Two genes encoding microbody malate dehydrogenase from Brassica napus."
    Imhoff U., Voetz M., Wingender R., Schnabl H., Wolf N.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiMDHG1_BRANA
AccessioniPrimary (citable) accession number: Q43743
Secondary accession number(s): Q9T0L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 1, 2000
Last modified: January 7, 2015
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.