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Q43743 (MDHG1_BRANA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Malate dehydrogenase 1, glyoxysomal
EC=1.1.1.37
Gene names
Name:MDH1
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Glyoxysome.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentGlyoxysome
Peroxisome
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-KW

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentglyoxysome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3838Glyoxysome Potential
Chain39 – 358320Malate dehydrogenase 1, glyoxysomal
PRO_0000018636

Regions

Nucleotide binding53 – 597NAD By similarity
Nucleotide binding162 – 1643NAD By similarity

Sites

Active site2221Proton acceptor By similarity
Binding site791NAD By similarity
Binding site1261Substrate By similarity
Binding site1321Substrate By similarity
Binding site1391NAD By similarity
Binding site1641Substrate By similarity
Binding site1981Substrate By similarity
Binding site2731NAD By similarity

Experimental info

Sequence conflict1 – 88MEFRGDAY → MPH Ref.1
Sequence conflict681M → V in CAA63268. Ref.1
Sequence conflict721V → G in CAA63268. Ref.1
Sequence conflict151 – 1522AK → GG in CAA63268. Ref.1
Sequence conflict2311L → S in CAA63268. Ref.1
Sequence conflict2351K → T in CAA63268. Ref.1
Sequence conflict2511R → K in CAA63268. Ref.1
Sequence conflict2541N → H in CAA63268. Ref.1
Sequence conflict2581E → D in CAA63268. Ref.1
Sequence conflict2631K → H in CAA63268. Ref.1
Sequence conflict2661A → V in CAA63268. Ref.1
Sequence conflict269 – 27911ATLSMAYAAAK → SPLPIILAAP in CAA63268. Ref.1
Sequence conflict306 – 3116ELAFFA → DYFLC in CAA63268. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q43743 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: DC94CBC76ACEEE4A

FASTA35837,732
        10         20         30         40         50         60 
MEFRGDAYKR IAMISAHLQP SFTPQMEAKN SVMGLESCRA KGGNPGFKVA ILGAAGGIGQ 

        70         80         90        100        110        120 
SLSLLMKMNP LVSLLHLYDV VNAPGVTADV SHMDTGAVVR GFLGAKQLED ALTGMDLVII 

       130        140        150        160        170        180 
PAGVPRKPGM TRDDLFKINA GIVRTLCEGV AKCCPNAIVN LISNPVNSTV AIAAEVFKKA 

       190        200        210        220        230        240 
GTYDPKKLLG VTTLDVARAN TFVAEVLGLD PREVDVPVVG GHAGVTILPL LSQVKPPSSF 

       250        260        270        280        290        300 
TPSEIEYLTN RIQNGGTEVV EAKAGAGSAT LSMAYAAAKF ADACLRGLRG DANVIECSFV 

       310        320        330        340        350 
ASQVTELAFF ATKVRLGRTG AEEVFQLGPL NEYERVGLEK AKEELAGSIQ KGVDFIRK

« Hide

References

[1]"A full-length cDNA coding for glyoxysomal malate dehydrogenase from Brassica napus L."
Witt U., Rehberg S., Abel W.O.
Plant Gene Register PGR95-124
Cited for: NUCLEOTIDE SEQUENCE.
Strain: cv. Andor.
Tissue: Leaf.
[2]"Two genes encoding microbody malate dehydrogenase from Brassica napus."
Imhoff U., Voetz M., Wingender R., Schnabl H., Wolf N.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X92512 mRNA. Translation: CAA63268.1.
AJ242712 Genomic DNA. Translation: CAB43994.1.
PIRT08015.

3D structure databases

ProteinModelPortalQ43743.
SMRQ43743. Positions 46-357.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PTHR11540:SF1. PTHR11540:SF1. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDHG1_BRANA
AccessionPrimary (citable) accession number: Q43743
Secondary accession number(s): Q9T0L8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 1, 2000
Last modified: May 29, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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