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Protein

Malate dehydrogenase 1, glyoxysomal

Gene

MDH1

Organism
Brassica napus (Rape)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79NADBy similarity1
Binding sitei126SubstratePROSITE-ProRule annotation1
Binding sitei132SubstratePROSITE-ProRule annotation1
Binding sitei139NADBy similarity1
Binding sitei164SubstratePROSITE-ProRule annotation1
Binding sitei198SubstratePROSITE-ProRule annotation1
Active sitei222Proton acceptorBy similarity1
Binding sitei273NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 59NADBy similarity7
Nucleotide bindingi162 – 164NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase 1, glyoxysomal (EC:1.1.1.37)
Gene namesi
Name:MDH1
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 38GlyoxysomeSequence analysisAdd BLAST38
ChainiPRO_000001863639 – 358Malate dehydrogenase 1, glyoxysomalAdd BLAST320

Proteomic databases

PRIDEiQ43743.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ43743.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43743-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFRGDAYKR IAMISAHLQP SFTPQMEAKN SVMGLESCRA KGGNPGFKVA
60 70 80 90 100
ILGAAGGIGQ SLSLLMKMNP LVSLLHLYDV VNAPGVTADV SHMDTGAVVR
110 120 130 140 150
GFLGAKQLED ALTGMDLVII PAGVPRKPGM TRDDLFKINA GIVRTLCEGV
160 170 180 190 200
AKCCPNAIVN LISNPVNSTV AIAAEVFKKA GTYDPKKLLG VTTLDVARAN
210 220 230 240 250
TFVAEVLGLD PREVDVPVVG GHAGVTILPL LSQVKPPSSF TPSEIEYLTN
260 270 280 290 300
RIQNGGTEVV EAKAGAGSAT LSMAYAAAKF ADACLRGLRG DANVIECSFV
310 320 330 340 350
ASQVTELAFF ATKVRLGRTG AEEVFQLGPL NEYERVGLEK AKEELAGSIQ

KGVDFIRK
Length:358
Mass (Da):37,732
Last modified:December 1, 2000 - v2
Checksum:iDC94CBC76ACEEE4A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 8MEFRGDAY → MPH (Ref. 1) Curated8
Sequence conflicti68M → V in CAA63268 (Ref. 1) Curated1
Sequence conflicti72V → G in CAA63268 (Ref. 1) Curated1
Sequence conflicti151 – 152AK → GG in CAA63268 (Ref. 1) Curated2
Sequence conflicti231L → S in CAA63268 (Ref. 1) Curated1
Sequence conflicti235K → T in CAA63268 (Ref. 1) Curated1
Sequence conflicti251R → K in CAA63268 (Ref. 1) Curated1
Sequence conflicti254N → H in CAA63268 (Ref. 1) Curated1
Sequence conflicti258E → D in CAA63268 (Ref. 1) Curated1
Sequence conflicti263K → H in CAA63268 (Ref. 1) Curated1
Sequence conflicti266A → V in CAA63268 (Ref. 1) Curated1
Sequence conflicti269 – 279ATLSMAYAAAK → SPLPIILAAP in CAA63268 (Ref. 1) CuratedAdd BLAST11
Sequence conflicti306 – 311ELAFFA → DYFLC in CAA63268 (Ref. 1) Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92512 mRNA. Translation: CAA63268.1.
AJ242712 Genomic DNA. Translation: CAB43994.1.
PIRiT08015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92512 mRNA. Translation: CAA63268.1.
AJ242712 Genomic DNA. Translation: CAB43994.1.
PIRiT08015.

3D structure databases

ProteinModelPortaliQ43743.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ43743.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDHG1_BRANA
AccessioniPrimary (citable) accession number: Q43743
Secondary accession number(s): Q9T0L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.