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Protein

Peroxidase 27

Gene

PER27

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei62 – 621Transition state stabilizerPROSITE-ProRule annotation
Active sitei66 – 661Proton acceptor
Metal bindingi67 – 671Calcium 1PROSITE-ProRule annotation
Metal bindingi70 – 701Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi72 – 721Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi74 – 741Calcium 1PROSITE-ProRule annotation
Metal bindingi76 – 761Calcium 1PROSITE-ProRule annotation
Binding sitei159 – 1591Substrate; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi189 – 1891Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi190 – 1901Calcium 2PROSITE-ProRule annotation
Metal bindingi240 – 2401Calcium 2PROSITE-ProRule annotation
Metal bindingi243 – 2431Calcium 2PROSITE-ProRule annotation
Metal bindingi248 – 2481Calcium 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G01190-MONOMER.

Protein family/group databases

PeroxiBasei120. AtPrx27.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase 27 (EC:1.11.1.7)
Short name:
Atperox P27
Alternative name(s):
ATP12a
PRXR7
Gene namesi
Name:PER27
Synonyms:P27
Ordered Locus Names:At3g01190
ORF Names:T4P13.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G01190.

Subcellular locationi

  • Secreted PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 321298Peroxidase 27PRO_0000023693Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi35 ↔ 113PROSITE-ProRule annotation
Disulfide bondi68 ↔ 73PROSITE-ProRule annotation
Disulfide bondi119 ↔ 317PROSITE-ProRule annotation
Glycosylationi164 – 1641N-linked (GlcNAc...)Sequence analysis
Disulfide bondi196 ↔ 228PROSITE-ProRule annotation
Glycosylationi205 – 2051N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ43735.
PRIDEiQ43735.

Expressioni

Tissue specificityi

Expressed in the whole plant, but preferentially in roots and flowers.2 Publications

Inductioni

Up-regulated transiently by a cold treatment.1 Publication

Gene expression databases

GenevisibleiQ43735. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G01190.1.

Structurei

3D structure databases

ProteinModelPortaliQ43735.
SMRiQ43735. Positions 30-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IK6H. Eukaryota.
ENOG410YCC5. LUCA.
HOGENOMiHOG000237556.
InParanoidiQ43735.
KOiK00430.
OMAiTMLVVMC.
OrthoDBiEOG09360FKB.
PhylomeDBiQ43735.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43735-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASKRLVVS CLFLVLLFAQ ANSQGLKVGF YSKTCPQLEG IVKKVVFDAM
60 70 80 90 100
NKAPTLGAPL LRMFFHDCFV RGCDGSVLLD KPNNQGEKSA VPNLSLRGFG
110 120 130 140 150
IIDDSKAALE KVCPGIVSCS DILALVARDA MVALEGPSWE VETGRRDGRV
160 170 180 190 200
SNINEVNLPS PFDNITKLIS DFRSKGLNEK DLVILSGGHT IGMGHCPLLT
210 220 230 240 250
NRLYNFTGKG DSDPSLDSEY AAKLRKKCKP TDTTTALEMD PGSFKTFDLS
260 270 280 290 300
YFTLVAKRRG LFQSDAALLD NSKTRAYVLQ QIRTHGSMFF NDFGVSMVKM
310 320
GRTGVLTGKA GEIRKTCRSA N
Length:321
Mass (Da):34,950
Last modified:November 1, 1996 - v1
Checksum:i500BB40AF2F04F1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98319 mRNA. Translation: CAA66963.1.
X98775 mRNA. Translation: CAA67311.1.
AC008261 Genomic DNA. Translation: AAF26155.1.
CP002686 Genomic DNA. Translation: AEE73623.1.
AY062810 mRNA. Translation: AAL32888.1.
AY081573 mRNA. Translation: AAM10135.1.
AY087679 mRNA. Translation: AAM65216.1.
RefSeqiNP_186768.1. NM_110985.2.
UniGeneiAt.22518.

Genome annotation databases

EnsemblPlantsiAT3G01190.1; AT3G01190.1; AT3G01190.
GeneIDi821314.
GrameneiAT3G01190.1; AT3G01190.1; AT3G01190.
KEGGiath:AT3G01190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98319 mRNA. Translation: CAA66963.1.
X98775 mRNA. Translation: CAA67311.1.
AC008261 Genomic DNA. Translation: AAF26155.1.
CP002686 Genomic DNA. Translation: AEE73623.1.
AY062810 mRNA. Translation: AAL32888.1.
AY081573 mRNA. Translation: AAM10135.1.
AY087679 mRNA. Translation: AAM65216.1.
RefSeqiNP_186768.1. NM_110985.2.
UniGeneiAt.22518.

3D structure databases

ProteinModelPortaliQ43735.
SMRiQ43735. Positions 30-321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G01190.1.

Protein family/group databases

PeroxiBasei120. AtPrx27.

Proteomic databases

PaxDbiQ43735.
PRIDEiQ43735.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G01190.1; AT3G01190.1; AT3G01190.
GeneIDi821314.
GrameneiAT3G01190.1; AT3G01190.1; AT3G01190.
KEGGiath:AT3G01190.

Organism-specific databases

TAIRiAT3G01190.

Phylogenomic databases

eggNOGiENOG410IK6H. Eukaryota.
ENOG410YCC5. LUCA.
HOGENOMiHOG000237556.
InParanoidiQ43735.
KOiK00430.
OMAiTMLVVMC.
OrthoDBiEOG09360FKB.
PhylomeDBiQ43735.

Enzyme and pathway databases

BioCyciARA:AT3G01190-MONOMER.

Miscellaneous databases

PROiQ43735.

Gene expression databases

GenevisibleiQ43735. AT.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPER27_ARATH
AccessioniPrimary (citable) accession number: Q43735
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.