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Protein

Peroxidase 50

Gene

PER50

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Exhibits a Ca2+-pectate binding affinity which could be interpreted in vivo as a specificity to interact with the pectic structure of the cell wall.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei63Transition state stabilizerPROSITE-ProRule annotation1
Active sitei67Proton acceptorPROSITE-ProRule annotation1
Metal bindingi68Calcium 1PROSITE-ProRule annotation1
Metal bindingi71Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi73Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi75Calcium 1PROSITE-ProRule annotation1
Metal bindingi77Calcium 1PROSITE-ProRule annotation1
Binding sitei167Substrate; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi197Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi198Calcium 2PROSITE-ProRule annotation1
Metal bindingi249Calcium 2PROSITE-ProRule annotation1
Metal bindingi252Calcium 2PROSITE-ProRule annotation1
Metal bindingi257Calcium 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Biological rhythms, Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT4G37520-MONOMER.

Protein family/group databases

PeroxiBasei216. AtPrx50.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase 50 (EC:1.11.1.7)
Short name:
Atperox P50
Alternative name(s):
ATP9a
PRXR2
Gene namesi
Name:PER50
Synonyms:P50
Ordered Locus Names:At4g37520
ORF Names:F19F18.10, F6G17.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G37520.

Subcellular locationi

  • Secreted PROSITE-ProRule annotation

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000002371526 – 329Peroxidase 50Add BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 119PROSITE-ProRule annotation
Disulfide bondi69 ↔ 74PROSITE-ProRule annotation
Disulfide bondi125 ↔ 325PROSITE-ProRule annotation
Disulfide bondi204 ↔ 236PROSITE-ProRule annotation
Glycosylationi215N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ43731.

Expressioni

Tissue specificityi

Expressed in roots and leaves.

Developmental stagei

Up-regulated during leaf development.1 Publication

Inductioni

Enhanced expression in response of mechanical wounding. Induced either by incompatible fungal pathogen attack, or by methyl jasmonate, a plant defense-related signaling molecule. Expressed under a diurnal rhythm (circadian clock control).2 Publications

Gene expression databases

ExpressionAtlasiQ43731. baseline and differential.
GenevisibleiQ43731. AT.

Interactioni

Protein-protein interaction databases

BioGridi15188. 1 interactor.
IntActiQ43731. 1 interactor.
STRINGi3702.AT4G37520.1.

Structurei

3D structure databases

ProteinModelPortaliQ43731.
SMRiQ43731.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IJ72. Eukaryota.
ENOG4111FDS. LUCA.
HOGENOMiHOG000237556.
InParanoidiQ43731.
KOiK00430.
OMAiNSMIKLG.
OrthoDBiEOG09360FB9.
PhylomeDBiQ43731.

Family and domain databases

CDDicd00693. secretory_peroxidase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019793. Peroxidases_heam-ligand_BS.
IPR033905. Secretory_peroxidase.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q43731-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVVVNKTNLL LLLLSLCLTL DLSSAQLRRN FYAGSCPNVE QIVRNAVQKK
60 70 80 90 100
VQQTFTTIPA TLRLYFHDCF VNGCDASVMI ASTNNNKAEK DHEENLSLAG
110 120 130 140 150
DGFDTVIKAK EALDAVPNCR NKVSCADILT MATRDVVNLA GGPQYDVELG
160 170 180 190 200
RLDGLSSTAA SVGGKLPHPT DDVNKLTSLF AKNGLSLNDM IALSGAHTLG
210 220 230 240 250
FAHCTKVFNR IYTFNKTTKV DPTVNKDYVT ELKASCPRNI DPRVAINMDP
260 270 280 290 300
TTPRQFDNVY YKNLQQGKGL FTSDQVLFTD RRSKPTVDLW ANNGQLFNQA
310 320
FINSMIKLGR VGVKTGSNGN IRRDCGAFN
Length:329
Mass (Da):36,076
Last modified:November 1, 1996 - v1
Checksum:i167FDDA36B279B1C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109A → T in AAM63630 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98314 mRNA. Translation: CAA66958.1.
AL035601 Genomic DNA. No translation available.
AL035605 Genomic DNA. Translation: CAB38291.1.
AL161591 Genomic DNA. Translation: CAB80417.1.
CP002687 Genomic DNA. Translation: AEE86804.1.
AY062816 mRNA. Translation: AAL32894.1.
AY081577 mRNA. Translation: AAM10139.1.
AY086567 mRNA. Translation: AAM63630.1.
X98856 Genomic DNA. Translation: CAA67362.1.
AJ006960 Genomic DNA. Translation: CAA07352.1.
AF083762 mRNA. Translation: AAN60320.1.
PIRiT04709.
RefSeqiNP_195468.1. NM_119916.4. [Q43731-1]
UniGeneiAt.22541.

Genome annotation databases

EnsemblPlantsiAT4G37520.1; AT4G37520.1; AT4G37520. [Q43731-1]
GeneIDi829907.
GrameneiAT4G37520.1; AT4G37520.1; AT4G37520.
KEGGiath:AT4G37520.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98314 mRNA. Translation: CAA66958.1.
AL035601 Genomic DNA. No translation available.
AL035605 Genomic DNA. Translation: CAB38291.1.
AL161591 Genomic DNA. Translation: CAB80417.1.
CP002687 Genomic DNA. Translation: AEE86804.1.
AY062816 mRNA. Translation: AAL32894.1.
AY081577 mRNA. Translation: AAM10139.1.
AY086567 mRNA. Translation: AAM63630.1.
X98856 Genomic DNA. Translation: CAA67362.1.
AJ006960 Genomic DNA. Translation: CAA07352.1.
AF083762 mRNA. Translation: AAN60320.1.
PIRiT04709.
RefSeqiNP_195468.1. NM_119916.4. [Q43731-1]
UniGeneiAt.22541.

3D structure databases

ProteinModelPortaliQ43731.
SMRiQ43731.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15188. 1 interactor.
IntActiQ43731. 1 interactor.
STRINGi3702.AT4G37520.1.

Protein family/group databases

PeroxiBasei216. AtPrx50.

Proteomic databases

PaxDbiQ43731.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G37520.1; AT4G37520.1; AT4G37520. [Q43731-1]
GeneIDi829907.
GrameneiAT4G37520.1; AT4G37520.1; AT4G37520.
KEGGiath:AT4G37520.

Organism-specific databases

TAIRiAT4G37520.

Phylogenomic databases

eggNOGiENOG410IJ72. Eukaryota.
ENOG4111FDS. LUCA.
HOGENOMiHOG000237556.
InParanoidiQ43731.
KOiK00430.
OMAiNSMIKLG.
OrthoDBiEOG09360FB9.
PhylomeDBiQ43731.

Enzyme and pathway databases

BioCyciARA:AT4G37520-MONOMER.

Miscellaneous databases

PROiQ43731.

Gene expression databases

ExpressionAtlasiQ43731. baseline and differential.
GenevisibleiQ43731. AT.

Family and domain databases

CDDicd00693. secretory_peroxidase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019793. Peroxidases_heam-ligand_BS.
IPR033905. Secretory_peroxidase.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPER50_ARATH
AccessioniPrimary (citable) accession number: Q43731
Secondary accession number(s): Q96523, Q9SBA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.