Q43729 (PER57_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxidase 57
Short name=Atperox P57
EC=1.11.1.7

Alternative name(s):
ATP13a
PRXR10

Gene names

Name:	PER57
Synonyms:	P57
Ordered Locus Names:	At5g17820
ORF Names:	MVA3.18, MVA3.170

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Catalytic activity	2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity. Binds 2 calcium ions per subunit By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Mainly expressed in roots. Ref.8
Induction	Positively light-regulated during the firt stage of development. Up-regulated transiently by a cold treatment. Induced in shoots of plants subjected to a long Fe deficiency stress. Down-regulated by salicylic acid, a plant defense-related signaling molecule. Ref.9 Ref.10 Ref.11 Ref.12
Miscellaneous	There are 73 peroxidase genes in A.thaliana.
Sequence similarities	Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.
Sequence caution	The sequence AAM65434.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from direct assay. Source: TAIR plant-type cell wall Inferred from direct assay. Source: TAIR plasmodesma Inferred from direct assay. Source: TAIR
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 313	291	Peroxidase 57		PRO_0000023722
Sites
	Active site	64	1	Proton acceptor By similarity
	Metal binding	65	1	Calcium 1 By similarity
	Metal binding	68	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	70	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	72	1	Calcium 1 By similarity
	Metal binding	74	1	Calcium 1 By similarity
	Metal binding	185	1	Iron (heme axial ligand) By similarity
	Metal binding	186	1	Calcium 2 By similarity
	Metal binding	233	1	Calcium 2 By similarity
	Metal binding	236	1	Calcium 2 By similarity
	Metal binding	241	1	Calcium 2 By similarity
	Binding site	155	1	Substrate; via carbonyl oxygen By similarity
	Site	60	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	23	1	Pyrrolidone carboxylic acid By similarity
	Disulfide bond	33 ↔ 109		By similarity
	Disulfide bond	66 ↔ 71		By similarity
	Disulfide bond	115 ↔ 309		By similarity
	Disulfide bond	192 ↔ 224		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43729 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BFD2855EC45DFF26	FASTA	313	34,098
	10 20 30 40 50 60 MMKGAKFSSL LVLFFIFPIA FAQLRVGFYS QSCPQAETIV RNLVRQRFGV TPTVTAALLR 70 80 90 100 110 120 MHFHDCFVKG CDASLLIDST NSEKTAGPNG SVREFDLIDR IKAQLEAACP STVSCADIVT 130 140 150 160 170 180 LATRDSVALA GGPSYSIPTG RRDGRVSNNL DVTLPGPTIS VSGAVSLFTN KGMNTFDAVA 190 200 210 220 230 240 LLGAHTVGQG NCGLFSDRIT SFQGTGRPDP SMDPALVTSL RNTCRNSATA ALDQSSPLRF 250 260 270 280 290 300 DNQFFKQIRK RRGVLQVDQR LASDPQTRGI VARYANNNAF FKRQFVRAMV KMGAVDVLTG 310 RNGEIRRNCR RFN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Eleven cDNA clones from Arabidopsis thaliana encoding isoperoxidases." Capelli N., Tognolli M., Flach J., Overney S., Penel C., Greppin H., Simon P. Plant Gene Register PGR96-066 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"From expressed sequence tags to structure, function, evolution and expression of 28 ER-targeted Arabidopsis peroxidases." Welinder K.G., Jespersen H.M., Kjaersgaard I.V.H., Justesen A.F., Oestergaard L., Abelskov A.K., Jensen R.B., Hansen L.N., Rasmussen S.K. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones." Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:291-300(1997) [PubMed: 9405937] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Computational analyses and annotations of the Arabidopsis peroxidase gene family." Oestergaard L., Pedersen A.G., Jespersen H.M., Brunak S., Welinder K.G. FEBS Lett. 433:98-102(1998) [PubMed: 9738941] [Abstract] Cited for: CHARACTERIZATION. Strain: cv. Columbia.
[8]	"Toward elucidating the global gene expression patterns of developing Arabidopsis: parallel analysis of 8300 genes by a high-density oligonucleotide probe array." Zhu T., Budworth P., Han B., Brown D., Chang H.-S., Zou G., Wang X. Plant Physiol. Biochem. 39:221-242(2001) Cited for: TISSUE SPECIFICITY. Strain: cv. Columbia.
[9]	"Differential gene expression in Arabidopsis monitored using cDNA arrays." Desprez T., Amselem J., Caboche M., Hoefte H. Plant J. 14:643-652(1998) [PubMed: 9675907] [Abstract] Cited for: INDUCTION. Strain: cv. Columbia.
[10]	"Coordinated plant defense responses in Arabidopsis revealed by microarray analysis." Schenk P.M., Kazan K., Wilson I., Anderson J.P., Richmond T., Somerville S.C., Manners J.M. Proc. Natl. Acad. Sci. U.S.A. 97:11655-11660(2000) [PubMed: 11027363] [Abstract] Cited for: INDUCTION. Strain: cv. Columbia.
[11]	"Response of Arabidopsis to iron deficiency stress as revealed by microarray analysis." Thimm O., Essigmann B., Kloska S., Altmann T., Buckhout T.J. Plant Physiol. 127:1030-1043(2001) [PubMed: 11706184] [Abstract] Cited for: INDUCTION. Strain: cv. Columbia.
[12]	"Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway." Fowler S., Thomashow M.F. Plant Cell 14:1675-1690(2002) [PubMed: 12172015] [Abstract] Cited for: INDUCTION. Strain: cv. Columbia.
[13]	"Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana." Tognolli M., Penel C., Greppin H., Simon P. Gene 288:129-138(2002) [PubMed: 12034502] [Abstract] Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98322 mRNA. Translation: CAA66966.1. X98776 mRNA. Translation: CAA67312.1. AB006706 Genomic DNA. Translation: BAB09581.1. CP002688 Genomic DNA. Translation: AED92474.1. BT002958 mRNA. Translation: AAO22769.2. AY087882 mRNA. Translation: AAM65434.1. Different initiation.
IPI	IPI00544734.
RefSeq	NP_197284.1. NM_121788.3.
UniGene	At.25535.
3D structure databases
ProteinModelPortal	Q43729.
SMR	Q43729. Positions 23-313.
ModBase	Search...
Protein-protein interaction databases
STRING	Q43729.
Protein family/group databases
PeroxiBase	223. AtPrx57.
Proteomic databases
PRIDE	Q43729.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G17820.1; AT5G17820.1; AT5G17820.
GeneID	831650.
GenomeReviews	Gene locus AT5G17820 in contig BA000015_GR.
KEGG	ath:AT5G17820.
Organism-specific databases
GeneFarm	1912. 61.
TAIR	At5g17820.
Phylogenomic databases
HOGENOM	HBG597790.
InParanoid	Q43729.
OMA	ARYANNN.
PhylomeDB	Q43729.
ProtClustDB	CLSN2913407.
Gene expression databases
ArrayExpress	Q43729.
Genevestigator	Q43729.
GermOnline	AT5G17820. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR000823. Peroxidase_pln. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K00430.
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00458. PEROXIDASE. PR00461. PLPEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PER57_ARATH

Accession

Primary (citable) accession number: Q43729
Secondary accession number(s): Q84WN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program