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Q43727

- G6PD1_ARATH

UniProt

Q43727 - G6PD1_ARATH

Protein

Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic

Gene

APG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 2 (11 Jul 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

    Catalytic activityi

    D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

    Enzyme regulationi

    Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei131 – 1311NADPBy similarity
    Binding sitei234 – 2341NADP; via carbonyl oxygenBy similarity
    Binding sitei234 – 2341SubstrateBy similarity
    Binding sitei302 – 3021SubstrateBy similarity
    Binding sitei321 – 3211SubstrateBy similarity
    Active sitei326 – 3261Proton acceptorBy similarity
    Binding sitei422 – 4221SubstrateBy similarity
    Binding sitei427 – 4271SubstrateBy similarity
    Binding sitei463 – 4631SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi97 – 1048NADPBy similarity

    GO - Molecular functioni

    1. glucose-6-phosphate dehydrogenase activity Source: TAIR
    2. NADP binding Source: InterPro
    3. protein binding Source: TAIR

    GO - Biological processi

    1. glucose metabolic process Source: TAIR
    2. pentose-phosphate shunt, oxidative branch Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00115; UER00408.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic (EC:1.1.1.49)
    Short name:
    G6PD1
    Short name:
    G6PDH1
    Gene namesi
    Name:APG1
    Ordered Locus Names:At5g35790
    ORF Names:MIK22.2, MWP19.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G35790.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5050ChloroplastSequence AnalysisAdd
    BLAST
    Chaini51 – 576526Glucose-6-phosphate 1-dehydrogenase 1, chloroplasticPRO_0000010435Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi149 ↔ 157Redox modulationBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ43727.
    PRIDEiQ43727.

    Expressioni

    Developmental stagei

    Increase of activity in the apex linked to the early stages of the transition from vegetative to reproductive growth.

    Gene expression databases

    GenevestigatoriQ43727.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi18812. 3 interactions.
    IntActiQ43727. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ43727.
    SMRiQ43727. Positions 91-556.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni264 – 2685Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0364.
    HOGENOMiHOG000046192.
    InParanoidiQ43727.
    KOiK00036.
    OMAiKGGKTYP.
    PhylomeDBiQ43727.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00966. G6PD.
    InterProiIPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000110. G6PD. 1 hit.
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR00871. zwf. 1 hit.
    PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q43727-1 [UniParc]FASTAAdd to Basket

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    MATHSMIIPS PSSSSSSLAT AASPFKETLP LFSRSLTFPR KSLFSQVRLR    50
    FFAEKHSQLD TSNGCATNFA SLQDSGDQLT EEHVTKGEST LSITVVGASG 100
    DLAKKKIFPA LFALFYEGCL PQDFSVFGYA RTKLTHEELR DMISSTLTCR 150
    IDQREKCGDK MEQFLKRCFY HSGQYNSEED FAELNKKLKE KEAGKISNRL 200
    YYLSIPPNIF VDVVRCASLR ASSENGWTRV IVEKPFGRDS ESSGELTRCL 250
    KQYLTEEQIF RIDHYLGKEL VENLSVLRFS NLVFEPLWSR NYIRNVQLIF 300
    SEDFGTEGRG GYFDQYGIIR DIMQNHLLQI LALFAMETPV SLDAEDIRSE 350
    KVKVLRSMKP LRLEDVVVGQ YKGHNKGGKT YPGYTDDPTV PNHSLTPTFA 400
    AAAMFINNAR WDGVPFLMKA GKALHTRGAE IRVQFRHVPG NLYKKSFATN 450
    LDNATNELVI RVQPDEGIYL RINNKVPGLG MRLDRSDLNL LYRSRYPREI 500
    PDAYERLLLD AIEGERRLFI RSDELDAAWD LFTPALKELE EKKIIPELYP 550
    YGSRGPVGAH YLASKYNVRW GDLGEA 576
    Length:576
    Mass (Da):65,428
    Last modified:July 11, 2002 - v2
    Checksum:i6559FFCCF76F44DF
    GO

    Sequence cautioni

    The sequence CAA59012.1 differs from that shown. Reason: Frameshift at position 65.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti110 – 1101A → D in CAA59012. 1 PublicationCurated
    Sequence conflicti205 – 2139IPPNIFVDV → STPKLLVDE in CAA04696. (PubMed:10437832)Curated
    Sequence conflicti297 – 2971Q → E in CAA59012. 1 PublicationCurated
    Sequence conflicti383 – 3831G → A in CAA59012. 1 PublicationCurated
    Sequence conflicti485 – 4862RS → PR in CAA04696. (PubMed:10437832)Curated
    Sequence conflicti514 – 5141G → A in CAA04696. (PubMed:10437832)Curated
    Sequence conflicti530 – 5301D → E in CAA04696. (PubMed:10437832)Curated
    Sequence conflicti539 – 5391L → R in CAA04696. (PubMed:10437832)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001359 mRNA. Translation: CAA04696.1.
    AB005236 Genomic DNA. Translation: BAB09918.1.
    CP002688 Genomic DNA. Translation: AED94018.1.
    AY099561 mRNA. Translation: AAM20413.1.
    BT002133 mRNA. Translation: AAN72144.1.
    AY086213 mRNA. Translation: AAM64291.1.
    X84230 mRNA. Translation: CAA59012.1. Frameshift.
    RefSeqiNP_198428.1. NM_122970.5.
    UniGeneiAt.60.

    Genome annotation databases

    EnsemblPlantsiAT5G35790.1; AT5G35790.1; AT5G35790.
    GeneIDi833559.
    KEGGiath:AT5G35790.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001359 mRNA. Translation: CAA04696.1 .
    AB005236 Genomic DNA. Translation: BAB09918.1 .
    CP002688 Genomic DNA. Translation: AED94018.1 .
    AY099561 mRNA. Translation: AAM20413.1 .
    BT002133 mRNA. Translation: AAN72144.1 .
    AY086213 mRNA. Translation: AAM64291.1 .
    X84230 mRNA. Translation: CAA59012.1 . Frameshift.
    RefSeqi NP_198428.1. NM_122970.5.
    UniGenei At.60.

    3D structure databases

    ProteinModelPortali Q43727.
    SMRi Q43727. Positions 91-556.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 18812. 3 interactions.
    IntActi Q43727. 1 interaction.

    Proteomic databases

    PaxDbi Q43727.
    PRIDEi Q43727.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G35790.1 ; AT5G35790.1 ; AT5G35790 .
    GeneIDi 833559.
    KEGGi ath:AT5G35790.

    Organism-specific databases

    TAIRi AT5G35790.

    Phylogenomic databases

    eggNOGi COG0364.
    HOGENOMi HOG000046192.
    InParanoidi Q43727.
    KOi K00036.
    OMAi KGGKTYP.
    PhylomeDBi Q43727.

    Enzyme and pathway databases

    UniPathwayi UPA00115 ; UER00408 .

    Gene expression databases

    Genevestigatori Q43727.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00966. G6PD.
    InterProi IPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23429. PTHR23429. 1 hit.
    Pfami PF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000110. G6PD. 1 hit.
    PRINTSi PR00079. G6PDHDRGNASE.
    TIGRFAMsi TIGR00871. zwf. 1 hit.
    PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evidence for functional convergence of redox regulation in G6PDH isoforms of cyanobacteria and higher plants."
      Wendt U.K., Hauschild R., Lange C., Pietersma M., Wenderoth I., von Schaewen A.
      Plant Mol. Biol. 40:487-494(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
      Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
      DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Nucleotide sequence of a cDNA encoding the glucose-6-phosphate dehydrogenase from Arabidopsis thaliana."
      Fink A., Greppin H., Tacchini P.
      Plant Gene Register PGR95-021
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-576.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiG6PD1_ARATH
    AccessioniPrimary (citable) accession number: Q43727
    Secondary accession number(s): O65577, Q9FFM5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: July 11, 2002
    Last modified: October 1, 2014
    This is version 133 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3