Reviewed, UniProtKB/Swiss-Prot Q43727 (G6PD1_ARATH)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic
      Short name=G6PDH1
      Short name=G6PD1
    EC=1.1.1.49

Gene names

Name:	APG1
Ordered Locus Names:	At5g35790
ORF Names:	MIK22.2, MWP19.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.
Catalytic activity	D-glucose 6-phosphate + NADP(+) = D-glucono-1,5-lactone 6-phosphate + NADPH.
Enzyme regulation	Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast.
Developmental stage	Increase of activity in the apex linked to the early stages of the transition from vegetative to reproductive growth.
Miscellaneous	There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.
Sequence caution	The sequence CAA59012.1 differs from that shown. Reason: Frameshift at position 65.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt, oxidative branch Inferred from direct assay. Source: TAIR
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 50	50	Chloroplast Potential
	Chain	51 – 576	526	Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic		PRO_0000010435
Sites
	Active site	326	1	Proton acceptor By similarity
	Binding site	99	1	NADP By similarity
	Binding site	131	1	NADP By similarity
	Binding site	264	1	Substrate By similarity
	Binding site	268	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	149 ↔ 157		Redox modulation By similarity
Experimental info
	Sequence conflict	110	1	A → D in CAA59012. Ref.5
	Sequence conflict	205 – 213	9	IPPNIFVDV → STPKLLVDE in CAA04696. Ref.1
	Sequence conflict	297	1	Q → E in CAA59012. Ref.5
	Sequence conflict	383	1	G → A in CAA59012. Ref.5
	Sequence conflict	485 – 486	2	RS → PR in CAA04696. Ref.1
	Sequence conflict	514	1	G → A in CAA04696. Ref.1
	Sequence conflict	530	1	D → E in CAA04696. Ref.1
	Sequence conflict	539	1	L → R in CAA04696. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q43727-1 [UniParc]. Last modified July 11, 2002. Version 2. Checksum: 6559FFCCF76F44DF	FASTA	576	65,428
	10 20 30 40 50 60 MATHSMIIPS PSSSSSSLAT AASPFKETLP LFSRSLTFPR KSLFSQVRLR FFAEKHSQLD 70 80 90 100 110 120 TSNGCATNFA SLQDSGDQLT EEHVTKGEST LSITVVGASG DLAKKKIFPA LFALFYEGCL 130 140 150 160 170 180 PQDFSVFGYA RTKLTHEELR DMISSTLTCR IDQREKCGDK MEQFLKRCFY HSGQYNSEED 190 200 210 220 230 240 FAELNKKLKE KEAGKISNRL YYLSIPPNIF VDVVRCASLR ASSENGWTRV IVEKPFGRDS 250 260 270 280 290 300 ESSGELTRCL KQYLTEEQIF RIDHYLGKEL VENLSVLRFS NLVFEPLWSR NYIRNVQLIF 310 320 330 340 350 360 SEDFGTEGRG GYFDQYGIIR DIMQNHLLQI LALFAMETPV SLDAEDIRSE KVKVLRSMKP 370 380 390 400 410 420 LRLEDVVVGQ YKGHNKGGKT YPGYTDDPTV PNHSLTPTFA AAAMFINNAR WDGVPFLMKA 430 440 450 460 470 480 GKALHTRGAE IRVQFRHVPG NLYKKSFATN LDNATNELVI RVQPDEGIYL RINNKVPGLG 490 500 510 520 530 540 MRLDRSDLNL LYRSRYPREI PDAYERLLLD AIEGERRLFI RSDELDAAWD LFTPALKELE 550 560 570 EKKIIPELYP YGSRGPVGAH YLASKYNVRW GDLGEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for functional convergence of redox regulation in G6PDH isoforms of cyanobacteria and higher plants." Wendt U.K., Hauschild R., Lange C., Pietersma M., Wenderoth I., von Schaewen A. Plant Mol. Biol. 40:487-494(1999) [PubMed: 10437832] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Nucleotide sequence of a cDNA encoding the glucose-6-phosphate dehydrogenase from Arabidopsis thaliana." Fink A., Greppin H., Tacchini P. (er) Plant Gene Register PGR95-021 Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-576. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ001359 mRNA. Translation: CAA04696.1. AB005236 Genomic DNA. Translation: BAB09918.1. AY099561 mRNA. Translation: AAM20413.1. BT002133 mRNA. Translation: AAN72144.1. AY086213 mRNA. Translation: AAM64291.1. X84230 mRNA. Translation: CAA59012.1. Frameshift.
RefSeq	NP_198428.1.
UniGene	At.60
3D structure databases
HSSP	HSSP built from PDB template 1QKI based on UniProtKB P11413.
ModBase	Search...
Proteomic databases
PRIDE	Q43727.
Genome annotation databases
GeneID	833559.
GenomeReviews	Gene locus AT5G35790 in contig BA000015_GR.
KEGG	ath:AT5G35790.
NMPDR	fig|3702.1.peg.25285.
Organism-specific databases
TAIR	At5g35790.
Gene expression databases
ArrayExpress	Q43727.
GermOnline	AT5G35790. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001282. Glc-6-P_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
ProDom	PD001129. G6PD. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PD1_ARATH

Accession

Primary (citable) accession number: Q43727
Secondary accession number(s): O65577, Q9FFM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 11, 2002
Last modified:	December 16, 2008
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)