Q43727 (G6PD1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic
Short name=G6PD1
Short name=G6PDH1
EC=1.1.1.49

Gene names

Name:	APG1
Ordered Locus Names:	At5g35790
ORF Names:	MIK22.2, MWP19.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.
Catalytic activity	D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.
Enzyme regulation	Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast.
Developmental stage	Increase of activity in the apex linked to the early stages of the transition from vegetative to reproductive growth.
Miscellaneous	There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.
Sequence caution	The sequence CAA59012.1 differs from that shown. Reason: Frameshift at position 65.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pentose-phosphate shunt, oxidative branch Inferred from direct assay PubMed 15634201. Source: TAIR
Cellular_component	chloroplast stroma Inferred from direct assay PubMed 20061580. Source: TAIR
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activity Inferred from direct assay PubMed 15634201. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 50	50	Chloroplast Potential
	Chain	51 – 576	526	Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic		PRO_0000010435
Sites
	Active site	326	1	Proton acceptor By similarity
	Binding site	99	1	NADP By similarity
	Binding site	131	1	NADP By similarity
	Binding site	264	1	Substrate By similarity
	Binding site	268	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	149 ↔ 157		Redox modulation By similarity
Experimental info
	Sequence conflict	110	1	A → D in CAA59012. Ref.6
	Sequence conflict	205 – 213	9	IPPNIFVDV → STPKLLVDE in CAA04696. Ref.1
	Sequence conflict	297	1	Q → E in CAA59012. Ref.6
	Sequence conflict	383	1	G → A in CAA59012. Ref.6
	Sequence conflict	485 – 486	2	RS → PR in CAA04696. Ref.1
	Sequence conflict	514	1	G → A in CAA04696. Ref.1
	Sequence conflict	530	1	D → E in CAA04696. Ref.1
	Sequence conflict	539	1	L → R in CAA04696. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q43727 [UniParc]. Last modified July 11, 2002. Version 2. Checksum: 6559FFCCF76F44DF	FASTA	576	65,428
	10 20 30 40 50 60 MATHSMIIPS PSSSSSSLAT AASPFKETLP LFSRSLTFPR KSLFSQVRLR FFAEKHSQLD 70 80 90 100 110 120 TSNGCATNFA SLQDSGDQLT EEHVTKGEST LSITVVGASG DLAKKKIFPA LFALFYEGCL 130 140 150 160 170 180 PQDFSVFGYA RTKLTHEELR DMISSTLTCR IDQREKCGDK MEQFLKRCFY HSGQYNSEED 190 200 210 220 230 240 FAELNKKLKE KEAGKISNRL YYLSIPPNIF VDVVRCASLR ASSENGWTRV IVEKPFGRDS 250 260 270 280 290 300 ESSGELTRCL KQYLTEEQIF RIDHYLGKEL VENLSVLRFS NLVFEPLWSR NYIRNVQLIF 310 320 330 340 350 360 SEDFGTEGRG GYFDQYGIIR DIMQNHLLQI LALFAMETPV SLDAEDIRSE KVKVLRSMKP 370 380 390 400 410 420 LRLEDVVVGQ YKGHNKGGKT YPGYTDDPTV PNHSLTPTFA AAAMFINNAR WDGVPFLMKA 430 440 450 460 470 480 GKALHTRGAE IRVQFRHVPG NLYKKSFATN LDNATNELVI RVQPDEGIYL RINNKVPGLG 490 500 510 520 530 540 MRLDRSDLNL LYRSRYPREI PDAYERLLLD AIEGERRLFI RSDELDAAWD LFTPALKELE 550 560 570 EKKIIPELYP YGSRGPVGAH YLASKYNVRW GDLGEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for functional convergence of redox regulation in G6PDH isoforms of cyanobacteria and higher plants." Wendt U.K., Hauschild R., Lange C., Pietersma M., Wenderoth I., von Schaewen A. Plant Mol. Biol. 40:487-494(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Nucleotide sequence of a cDNA encoding the glucose-6-phosphate dehydrogenase from Arabidopsis thaliana." Fink A., Greppin H., Tacchini P. Plant Gene Register PGR95-021 Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-576. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ001359 mRNA. Translation: CAA04696.1. AB005236 Genomic DNA. Translation: BAB09918.1. CP002688 Genomic DNA. Translation: AED94018.1. AY099561 mRNA. Translation: AAM20413.1. BT002133 mRNA. Translation: AAN72144.1. AY086213 mRNA. Translation: AAM64291.1. X84230 mRNA. Translation: CAA59012.1. Frameshift.
IPI	IPI00537871.
RefSeq	NP_198428.1. NM_122970.5.
UniGene	At.60.
3D structure databases
ProteinModelPortal	Q43727.
SMR	Q43727. Positions 91-556.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q43727. 1 interaction.
Proteomic databases
PaxDb	Q43727.
PRIDE	Q43727.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G35790.1; AT5G35790.1; AT5G35790.
GeneID	833559.
KEGG	ath:AT5G35790.
Organism-specific databases
TAIR	At5g35790.
Phylogenomic databases
eggNOG	COG0364.
HOGENOM	HOG000046192.
InParanoid	Q43727.
KO	K00036.
OMA	AMEPPGH.
PhylomeDB	Q43727.
ProtClustDB	PLN02640.
Enzyme and pathway databases
UniPathway	UPA00115; UER00408.
Gene expression databases
ArrayExpress	Q43727.
Genevestigator	Q43727.
GermOnline	AT5G35790. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR001282. G6P_DH. IPR019796. G6P_DH_AS. IPR022675. G6P_DH_C. IPR022674. G6P_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR23429. PTHR23429. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PD1_ARATH

Accession

Primary (citable) accession number: Q43727
Secondary accession number(s): O65577, Q9FFM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 11, 2002
Last modified:	May 1, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program