Reviewed,
UniProtKB/Swiss-Prot Q43727 (G6PD1_ARATH)
Last modified
December 16, 2008.
Version 85.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
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Names and origin
| Protein names | Recommended name: Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic Short name=G6PDH1 Short name=G6PD1 EC=1.1.1.49 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 576 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division. |
| Catalytic activity | D-glucose 6-phosphate + NADP(+) = D-glucono-1,5-lactone 6-phosphate + NADPH. |
| Enzyme regulation | Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity. |
| Pathway | |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | |
| Developmental stage | Increase of activity in the apex linked to the early stages of the transition from vegetative to reproductive growth. |
| Miscellaneous | There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana. |
| Sequence similarities | Belongs to the glucose-6-phosphate dehydrogenase family. |
| Sequence caution | The sequence CAA59012.1 differs from that shown. Reason: Frameshift at position 65. |
Ontologies
Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Glucose metabolism |
| Cellular component | Chloroplast Plastid |
| Domain | Transit peptide |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt, oxidative branchInferred from direct assay. Source: TAIR |
| Cellular component | chloroplast Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 50 | 50 | Chloroplast Potential | ||||||||
| Chain | 51 – 576 | 526 | Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic | PRO_0000010435 | |||||||
Sites | |||||||||||
| Active site | 326 | 1 | Proton acceptor By similarity | ||||||||
| Binding site | 99 | 1 | NADP By similarity | ||||||||
| Binding site | 131 | 1 | NADP By similarity | ||||||||
| Binding site | 264 | 1 | Substrate By similarity | ||||||||
| Binding site | 268 | 1 | Substrate By similarity | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 149 ↔ 157 | Redox modulation By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 110 | 1 | A → D in CAA59012. Ref.5 | ||||||||
| Sequence conflict | 205 – 213 | 9 | IPPNIFVDV → STPKLLVDE in CAA04696. Ref.1 | ||||||||
| Sequence conflict | 297 | 1 | Q → E in CAA59012. Ref.5 | ||||||||
| Sequence conflict | 383 | 1 | G → A in CAA59012. Ref.5 | ||||||||
| Sequence conflict | 485 – 486 | 2 | RS → PR in CAA04696. Ref.1 | ||||||||
| Sequence conflict | 514 | 1 | G → A in CAA04696. Ref.1 | ||||||||
| Sequence conflict | 530 | 1 | D → E in CAA04696. Ref.1 | ||||||||
| Sequence conflict | 539 | 1 | L → R in CAA04696. Ref.1 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Evidence for functional convergence of redox regulation in G6PDH isoforms of cyanobacteria and higher plants." Wendt U.K., Hauschild R., Lange C., Pietersma M., Wenderoth I., von Schaewen A. Plant Mol. Biol. 40:487-494(1999) [PubMed: 10437832] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [4] | "Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [5] | "Nucleotide sequence of a cDNA encoding the glucose-6-phosphate dehydrogenase from Arabidopsis thaliana." Fink A., Greppin H., Tacchini P. (er) Plant Gene Register PGR95-021 Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-576. Strain: cv. Columbia. |
Cross-references
Sequence databases | |
|---|---|
| AJ001359 mRNA. Translation: CAA04696.1. AB005236 Genomic DNA. Translation: BAB09918.1. AY099561 mRNA. Translation: AAM20413.1. BT002133 mRNA. Translation: AAN72144.1. AY086213 mRNA. Translation: AAM64291.1. X84230 mRNA. Translation: CAA59012.1. Frameshift. | |
| RefSeq | NP_198428.1. |
| UniGene | At.60 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1QKI based on UniProtKB P11413. |
| ModBase | Search... |
Proteomic databases | |
| PRIDE | Q43727. |
Genome annotation databases | |
| GeneID | 833559. |
| GenomeReviews | Gene locus AT5G35790 in contig BA000015_GR. |
| KEGG | ath:AT5G35790. |
| NMPDR | fig|3702.1.peg.25285. |
Organism-specific databases | |
| TAIR | At5g35790. |
Gene expression databases | |
| ArrayExpress | Q43727. |
| GermOnline | AT5G35790. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR001282. Glc-6-P_DHase. IPR016040. NAD(P)-bd. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR23429. G6PDH. 1 hit. |
| Pfam | PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000110. G6PD. 1 hit. |
| PRINTS | PR00079. G6PDHDRGNASE. |
| ProDom | PD001129. G6PD. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR00871. zwf. 1 hit. |
| PROSITE | PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | G6PD1_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q43727 Secondary accession number(s): O65577, Q9FFM5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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