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Protein

Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic

Gene

APG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Enzyme regulationi

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation (By similarity).By similarity

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic (At1g24280), Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic (APG1), Glucose-6-phosphate 1-dehydrogenase (At3g27300), Glucose-6-phosphate 1-dehydrogenase (AXX17_At3g29760), Glucose-6-phosphate 1-dehydrogenase (G6PD4), Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic (At5g13110), Glucose-6-phosphate 1-dehydrogenase (AXX17_At1g25530), Glucose-6-phosphate 1-dehydrogenase (At5g35790), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g33080), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g33080), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g12520), Glucose-6-phosphate 1-dehydrogenase (G6PD5), Glucose-6-phosphate 1-dehydrogenase (G6PD5), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2 (ACG12), Glucose-6-phosphate 1-dehydrogenase (At1g09420), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g38480), Glucose-6-phosphate 1-dehydrogenase (AXX17_At1g09330), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 (ACG9), Glucose-6-phosphate 1-dehydrogenase (At5g40760), Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic (At1g09420)
  2. Probable 6-phosphogluconolactonase 5, chloroplastic (EMB2024), Probable 6-phosphogluconolactonase 3 (At5g24420), Probable 6-phosphogluconolactonase 4 (At5g24410), Probable 6-phosphogluconolactonase 1 (At1g13700), Probable 6-phosphogluconolactonase 2 (At3g49360)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At5g39410), 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At3g01580), 6-phosphogluconate dehydrogenase, decarboxylating 2, chloroplastic (At5g41670), 6-phosphogluconate dehydrogenase, decarboxylating 3 (At3g02360), 6-phosphogluconate dehydrogenase, decarboxylating 1, chloroplastic (At1g64190), 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At1g57660)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei131NADPBy similarity1
Binding sitei234NADP; via carbonyl oxygenBy similarity1
Binding sitei234SubstrateBy similarity1
Binding sitei302SubstrateBy similarity1
Binding sitei321SubstrateBy similarity1
Active sitei326Proton acceptorBy similarity1
Binding sitei422SubstrateBy similarity1
Binding sitei427SubstrateBy similarity1
Binding sitei463SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi97 – 104NADPBy similarity8

GO - Molecular functioni

  • glucose-6-phosphate dehydrogenase activity Source: TAIR
  • NADP binding Source: InterPro

GO - Biological processi

  • glucose metabolic process Source: TAIR
  • pentose-phosphate shunt, oxidative branch Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
LigandNADP

Enzyme and pathway databases

BRENDAi1.1.1.49 399
UniPathwayiUPA00115; UER00408

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic (EC:1.1.1.49)
Short name:
G6PD1
Short name:
G6PDH1
Gene namesi
Name:APG1
Ordered Locus Names:At5g35790
ORF Names:MIK22.2, MWP19.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G35790
TAIRilocus:2165154 AT5G35790

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 50ChloroplastSequence analysisAdd BLAST50
ChainiPRO_000001043551 – 576Glucose-6-phosphate 1-dehydrogenase 1, chloroplasticAdd BLAST526

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi149 ↔ 157Redox modulationBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ43727
PRIDEiQ43727

Expressioni

Developmental stagei

Increase of activity in the apex linked to the early stages of the transition from vegetative to reproductive growth.

Gene expression databases

ExpressionAtlasiQ43727 baseline and differential
GenevisibleiQ43727 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi18812, 4 interactors
IntActiQ43727, 1 interactor
STRINGi3702.AT5G35790.1

Structurei

3D structure databases

ProteinModelPortaliQ43727
SMRiQ43727
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni264 – 268Substrate bindingBy similarity5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0563 Eukaryota
COG0364 LUCA
HOGENOMiHOG000046192
InParanoidiQ43727
KOiK00036
OMAiTHTMIAH
OrthoDBiEOG093605BG
PhylomeDBiQ43727

Family and domain databases

HAMAPiMF_00966 G6PD, 1 hit
InterProiView protein in InterPro
IPR001282 G6P_DH
IPR019796 G6P_DH_AS
IPR022675 G6P_DH_C
IPR022674 G6P_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23429 PTHR23429, 1 hit
PfamiView protein in Pfam
PF02781 G6PD_C, 1 hit
PF00479 G6PD_N, 1 hit
PIRSFiPIRSF000110 G6PD, 1 hit
PRINTSiPR00079 G6PDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00871 zwf, 1 hit
PROSITEiView protein in PROSITE
PS00069 G6P_DEHYDROGENASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATHSMIIPS PSSSSSSLAT AASPFKETLP LFSRSLTFPR KSLFSQVRLR
60 70 80 90 100
FFAEKHSQLD TSNGCATNFA SLQDSGDQLT EEHVTKGEST LSITVVGASG
110 120 130 140 150
DLAKKKIFPA LFALFYEGCL PQDFSVFGYA RTKLTHEELR DMISSTLTCR
160 170 180 190 200
IDQREKCGDK MEQFLKRCFY HSGQYNSEED FAELNKKLKE KEAGKISNRL
210 220 230 240 250
YYLSIPPNIF VDVVRCASLR ASSENGWTRV IVEKPFGRDS ESSGELTRCL
260 270 280 290 300
KQYLTEEQIF RIDHYLGKEL VENLSVLRFS NLVFEPLWSR NYIRNVQLIF
310 320 330 340 350
SEDFGTEGRG GYFDQYGIIR DIMQNHLLQI LALFAMETPV SLDAEDIRSE
360 370 380 390 400
KVKVLRSMKP LRLEDVVVGQ YKGHNKGGKT YPGYTDDPTV PNHSLTPTFA
410 420 430 440 450
AAAMFINNAR WDGVPFLMKA GKALHTRGAE IRVQFRHVPG NLYKKSFATN
460 470 480 490 500
LDNATNELVI RVQPDEGIYL RINNKVPGLG MRLDRSDLNL LYRSRYPREI
510 520 530 540 550
PDAYERLLLD AIEGERRLFI RSDELDAAWD LFTPALKELE EKKIIPELYP
560 570
YGSRGPVGAH YLASKYNVRW GDLGEA
Length:576
Mass (Da):65,428
Last modified:July 11, 2002 - v2
Checksum:i6559FFCCF76F44DF
GO

Sequence cautioni

The sequence CAA59012 differs from that shown. Reason: Frameshift at position 65.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110A → D in CAA59012 (Ref. 6) Curated1
Sequence conflicti205 – 213IPPNIFVDV → STPKLLVDE in CAA04696 (PubMed:10437832).Curated9
Sequence conflicti297Q → E in CAA59012 (Ref. 6) Curated1
Sequence conflicti383G → A in CAA59012 (Ref. 6) Curated1
Sequence conflicti485 – 486RS → PR in CAA04696 (PubMed:10437832).Curated2
Sequence conflicti514G → A in CAA04696 (PubMed:10437832).Curated1
Sequence conflicti530D → E in CAA04696 (PubMed:10437832).Curated1
Sequence conflicti539L → R in CAA04696 (PubMed:10437832).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001359 mRNA Translation: CAA04696.1
AB005236 Genomic DNA Translation: BAB09918.1
CP002688 Genomic DNA Translation: AED94018.1
AY099561 mRNA Translation: AAM20413.1
BT002133 mRNA Translation: AAN72144.1
AY086213 mRNA Translation: AAM64291.1
X84230 mRNA Translation: CAA59012.1 Frameshift.
RefSeqiNP_198428.1, NM_122970.6
UniGeneiAt.60

Genome annotation databases

EnsemblPlantsiAT5G35790.1; AT5G35790.1; AT5G35790
GeneIDi833559
GrameneiAT5G35790.1; AT5G35790.1; AT5G35790
KEGGiath:AT5G35790

Similar proteinsi

Entry informationi

Entry nameiG6PD1_ARATH
AccessioniPrimary (citable) accession number: Q43727
Secondary accession number(s): O65577, Q9FFM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 11, 2002
Last modified: April 25, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health