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Protein

Cysteine synthase, mitochondrial

Gene

OASC

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a cysteine synthase. Plays a role in the sulfide detoxification in mitochondria.3 Publications

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.2 Publications

Cofactori

Kineticsi

  1. KM=0.98 mM for O(3)-acetyl-L-serine for the cysteine synthase activity2 Publications
  2. KM=0.43 mM for O(3)-acetyl-L-serine for the cysteine synthase activity2 Publications
  3. KM=4.7 µM for H2S for the cysteine synthase activity2 Publications
  1. Vmax=159 µmol/min/mg enzyme for L-cysteine for the cysteine synthase activity2 Publications
  2. Vmax=534 µmol/min/mg enzyme for L-cysteine for the cysteine synthase activity2 Publications
  3. Vmax=0.33 µmol/min/mg enzyme for H2S for the L-3-cyanoalanine synthase activity2 Publications

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serine acetyltransferase 3, mitochondrial (SAT3), Serine acetyltransferase 5 (SAT5), Serine acetyltransferase 1, chloroplastic (SAT1), Serine acetyltransferase 2 (SAT2), Serine acetyltransferase 4 (SAT4)
  2. Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2 (CYSD2), Bifunctional cystathionine gamma-lyase/cysteine synthase (DES1), Cysteine synthase, mitochondrial (OASC), Bifunctional L-3-cyanoalanine synthase/cysteine synthase D1 (CYSD1), Cysteine synthase, chloroplastic/chromoplastic (OASB), Cysteine synthase 1 (OASA1)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei185Pyridoxal phosphateBy similarity1
Binding sitei377Pyridoxal phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:AT3G59760-MONOMER.
UniPathwayiUPA00136; UER00200.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine synthase, mitochondrial (EC:2.5.1.47)
Alternative name(s):
Beta-substituted Ala synthase 2;2
Short name:
ARAth-Bsas2;2
CSase C
Short name:
AtCS-C
Short name:
CS-C
O-acetylserine (thiol)-lyase
O-acetylserine sulfhydrylase
OAS-TL C
Gene namesi
Name:OASC
Synonyms:ACS 1
Ordered Locus Names:At3g59760
ORF Names:F24G16.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G59760.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

No visible phenotype but displays lower levels of thiols in roots (PubMed:18024555). Growth retardation (PubMed:18223034). Defects in root hair formation (PubMed:22511607).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000006353? – 430Cysteine synthase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei154N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ43725.
PRIDEiQ43725.

PTM databases

SwissPalmiQ43725.

Expressioni

Gene expression databases

ExpressionAtlasiQ43725. baseline and differential.
GenevisibleiQ43725. AT.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with SAT3. Component of the cysteine synthase complex (CSC) composed of two OAS-TL dimers and one SAT hexamer.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SAT3Q392184EBI-1633616,EBI-1633440

Protein-protein interaction databases

BioGridi10459. 4 interactors.
DIPiDIP-40500N.
IntActiQ43725. 3 interactors.
STRINGi3702.AT3G59760.1.

Structurei

Secondary structure

1430
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi114 – 116Combined sources3
Helixi117 – 120Combined sources4
Beta strandi126 – 128Combined sources3
Helixi131 – 133Combined sources3
Beta strandi137 – 144Combined sources8
Helixi145 – 147Combined sources3
Helixi155 – 167Combined sources13
Turni173 – 175Combined sources3
Beta strandi177 – 181Combined sources5
Helixi185 – 197Combined sources13
Beta strandi200 – 206Combined sources7
Helixi211 – 219Combined sources9
Beta strandi223 – 227Combined sources5
Helixi229 – 231Combined sources3
Helixi232 – 246Combined sources15
Beta strandi250 – 252Combined sources3
Turni255 – 257Combined sources3
Helixi260 – 267Combined sources8
Helixi269 – 276Combined sources8
Beta strandi281 – 287Combined sources7
Beta strandi289 – 291Combined sources3
Helixi292 – 304Combined sources13
Beta strandi308 – 315Combined sources8
Helixi316 – 318Combined sources3
Helixi320 – 322Combined sources3
Turni345 – 347Combined sources3
Beta strandi349 – 354Combined sources6
Helixi356 – 370Combined sources15
Helixi376 – 388Combined sources13
Helixi392 – 394Combined sources3
Beta strandi398 – 403Combined sources6
Helixi407 – 410Combined sources4
Helixi414 – 422Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AECX-ray2.40A/B1-430[»]
ProteinModelPortaliQ43725.
SMRiQ43725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni289 – 293Pyridoxal phosphate bindingBy similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi49 – 54Poly-Ser6
Compositional biasi380 – 385Poly-Ala6

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1252. Eukaryota.
COG0031. LUCA.
HOGENOMiHOG000217394.
InParanoidiQ43725.
KOiK01738.
PhylomeDBiQ43725.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005859. CysK.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01139. cysK. 1 hit.
TIGR01136. cysKM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q43725-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVAMIMASRF NREAKLASQI LSTLLGNRSC YTSMAATSSS ALLLNPLTSS
60 70 80 90 100
SSSSTLRRFR CSPEISSLSF SSASDFSLAM KRQSRSFADG SERDPSVVCE
110 120 130 140 150
AVKRETGPDG LNIADNVSQL IGKTPMVYLN SIAKGCVANI AAKLEIMEPC
160 170 180 190 200
CSVKDRIGYS MVTDAEQKGF ISPGKSVLVE PTSGNTGIGL AFIAASRGYR
210 220 230 240 250
LILTMPASMS MERRVLLKAF GAELVLTDPA KGMTGAVQKA EEILKNTPDA
260 270 280 290 300
YMLQQFDNPA NPKIHYETTG PEIWDDTKGK VDIFVAGIGT GGTITGVGRF
310 320 330 340 350
IKEKNPKTQV IGVEPTESDI LSGGKPGPHK IQGIGAGFIP KNLDQKIMDE
360 370 380 390 400
VIAISSEEAI ETAKQLALKE GLMVGISSGA AAAAAIKVAK RPENAGKLIA
410 420 430
VVFPSFGERY LSTPLFQSIR EEVEKMQPEV
Length:430
Mass (Da):45,815
Last modified:January 16, 2004 - v3
Checksum:i9DDEB1B8E314E143
GO

Sequence cautioni

The sequence CAA57498 differs from that shown. Reason: Frameshift at positions 5, 424 and 426.Curated
The sequence CAB75795 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49Missing in CAA57498 (PubMed:10319184).Curated1
Sequence conflicti49Missing in CAB71290 (PubMed:10940562).Curated1
Sequence conflicti73A → G in CAA57498 (PubMed:10319184).Curated1
Sequence conflicti195Missing in CAA57498 (PubMed:10319184).Curated1
Sequence conflicti314E → R in CAA57498 (PubMed:10319184).Curated1
Sequence conflicti356 – 357SE → ISKL in CAA57498 (PubMed:10319184).Curated2
Sequence conflicti385Missing in CAA57498 (PubMed:10319184).Curated1
Sequence conflicti409Missing in CAA57498 (PubMed:10319184).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81973 mRNA. Translation: CAA57498.1. Frameshift.
AL138647 Genomic DNA. Translation: CAB75795.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79963.1.
AY099721 mRNA. Translation: AAM20572.1.
AY128885 mRNA. Translation: AAM91285.1.
AJ271727 mRNA. Translation: CAB71290.1.
PIRiT47800.
T52650.
RefSeqiNP_191535.2. NM_115838.6. [Q43725-1]
UniGeneiAt.25336.

Genome annotation databases

EnsemblPlantsiAT3G59760.3; AT3G59760.3; AT3G59760. [Q43725-1]
GeneIDi825145.
GrameneiAT3G59760.3; AT3G59760.3; AT3G59760.
KEGGiath:AT3G59760.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81973 mRNA. Translation: CAA57498.1. Frameshift.
AL138647 Genomic DNA. Translation: CAB75795.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79963.1.
AY099721 mRNA. Translation: AAM20572.1.
AY128885 mRNA. Translation: AAM91285.1.
AJ271727 mRNA. Translation: CAB71290.1.
PIRiT47800.
T52650.
RefSeqiNP_191535.2. NM_115838.6. [Q43725-1]
UniGeneiAt.25336.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AECX-ray2.40A/B1-430[»]
ProteinModelPortaliQ43725.
SMRiQ43725.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10459. 4 interactors.
DIPiDIP-40500N.
IntActiQ43725. 3 interactors.
STRINGi3702.AT3G59760.1.

PTM databases

SwissPalmiQ43725.

Proteomic databases

PaxDbiQ43725.
PRIDEiQ43725.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G59760.3; AT3G59760.3; AT3G59760. [Q43725-1]
GeneIDi825145.
GrameneiAT3G59760.3; AT3G59760.3; AT3G59760.
KEGGiath:AT3G59760.

Organism-specific databases

TAIRiAT3G59760.

Phylogenomic databases

eggNOGiKOG1252. Eukaryota.
COG0031. LUCA.
HOGENOMiHOG000217394.
InParanoidiQ43725.
KOiK01738.
PhylomeDBiQ43725.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00200.
BioCyciMetaCyc:AT3G59760-MONOMER.

Miscellaneous databases

PROiQ43725.

Gene expression databases

ExpressionAtlasiQ43725. baseline and differential.
GenevisibleiQ43725. AT.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005859. CysK.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01139. cysK. 1 hit.
TIGR01136. cysKM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSKM_ARATH
AccessioniPrimary (citable) accession number: Q43725
Secondary accession number(s): Q8L4A2, Q9M1Z8, Q9M440
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 16, 2004
Last modified: November 30, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.