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Reviewed, UniProtKB/Swiss-Prot Q43725 (CYSKM_ARATH)

Last modified November 3, 2009. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cysteine synthase, mitochondrial
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase
    CSase C
      Short name=CS-C
      Short name=AtCS-C
    O-acetylserine (thiol)-lyase
    OAS-TL C
Gene names
Name: OASC
Synonyms: ACS 1
Ordered Locus Names: At3g59760
ORF Names: F24G16.30
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length430 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.

Cofactor

Pyridoxal phosphate.

Pathway

Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.

Subcellular location

Mitochondrion. Ref.5

Sequence similarities

Belongs to the cysteine synthase/cystathionine beta-synthase family.

Sequence caution

The sequence CAA57498.1 differs from that shown. Reason: Frameshift at positions 5, 424 and 426.

The sequence CAB75795.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q43725-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 430Cysteine synthase, mitochondrialPRO_0000006353

Regions

Region289 – 2935Pyridoxal phosphate binding By similarity
Compositional bias49 – 546Poly-Ser
Compositional bias380 – 3856Poly-Ala

Sites

Binding site1851Pyridoxal phosphate By similarity
Binding site3771Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue1541N6-(pyridoxal phosphate)lysine By similarity

Experimental info

Sequence conflict491Missing in CAA57498. Ref.1
Sequence conflict491Missing in CAB71290. Ref.4
Sequence conflict731A → G in CAA57498. Ref.1
Sequence conflict1951Missing Ref.1
Sequence conflict3141E → R in CAA57498. Ref.1
Sequence conflict356 – 3572SE → ISKL in CAA57498. Ref.1
Sequence conflict3851Missing in CAA57498. Ref.1
Sequence conflict4091Missing in CAA57498. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 16, 2004. Version 3.
Checksum: 9DDEB1B8E314E143

FASTA43045,815
        10         20         30         40         50         60 
MVAMIMASRF NREAKLASQI LSTLLGNRSC YTSMAATSSS ALLLNPLTSS SSSSTLRRFR 

        70         80         90        100        110        120 
CSPEISSLSF SSASDFSLAM KRQSRSFADG SERDPSVVCE AVKRETGPDG LNIADNVSQL 

       130        140        150        160        170        180 
IGKTPMVYLN SIAKGCVANI AAKLEIMEPC CSVKDRIGYS MVTDAEQKGF ISPGKSVLVE 

       190        200        210        220        230        240 
PTSGNTGIGL AFIAASRGYR LILTMPASMS MERRVLLKAF GAELVLTDPA KGMTGAVQKA 

       250        260        270        280        290        300 
EEILKNTPDA YMLQQFDNPA NPKIHYETTG PEIWDDTKGK VDIFVAGIGT GGTITGVGRF 

       310        320        330        340        350        360 
IKEKNPKTQV IGVEPTESDI LSGGKPGPHK IQGIGAGFIP KNLDQKIMDE VIAISSEEAI 

       370        380        390        400        410        420 
ETAKQLALKE GLMVGISSGA AAAAAIKVAK RPENAGKLIA VVFPSFGERY LSTPLFQSIR 

       430 
EEVEKMQPEV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression analyses of mitochondrial and plastidic isoforms of cysteine synthase (O-acetylserine(thiol)lyase) from Arabidopsis thaliana."
Hesse H., Lipke J., Altmann T., Hofgen R.
Amino Acids 16:113-131(1999) [PubMed: 10319184] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Flower.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Genomic and functional characterization of the oas gene family encoding O-acetylserine (thiol) lyases, enzymes catalyzing the final step in cysteine biosynthesis in Arabidopsis thaliana."
Jost R., Berkowitz O., Wirtz M., Hopkins L., Hawkesford M.J., Hell R.
Gene 253:237-247(2000) [PubMed: 10940562] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 43-430.
Strain: cv. Columbia.
[5]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

X81973 mRNA. Translation: CAA57498.1. Frameshift.
AL138647 Genomic DNA. Translation: CAB75795.1. Sequence problems.
AY099721 mRNA. Translation: AAM20572.1.
AY128885 mRNA. Translation: AAM91285.1.
AJ271727 mRNA. Translation: CAB71290.1.
IPIIPI00530032.
PIRT47800.
T52650.
RefSeqNP_191535.2.
UniGeneAt.25336

3D structure databases

HSSPHSSP built from PDB template 1OAS based on UniProtKB P12674.
SMRQ43725. Positions 113-429.
ModBaseSearch...

Protein-protein interaction databases

IntActQ43725. 3 interactions.
STRINGQ43725.

Proteomic databases

PRIDEQ43725.

Genome annotation databases

GeneID825145.
GenomeReviewsGene locus AT3G59760 in contig BA000014_GR.
KEGGath:AT3G59760.
NMPDRfig|3702.1.peg.17330.

Organism-specific databases

TAIRAt3g59760.

Enzyme and pathway databases

BRENDA2.5.1.47. 302.

Gene expression databases

ArrayExpressQ43725.
GenevestigatorQ43725.

Family and domain databases

InterProIPR001216. Cys_synth_BS.
IPR005856. Cys_synthKM.
IPR005859. CysK.
IPR001926. PyrdxlP-dep_enz_bsu.
[Graphical view]
PfamPF00291. PALP. 1 hit.
[Graphical view]
TIGRFAMsTIGR01139. cysK. 1 hit.
TIGR01136. cysKM. 1 hit.
PROSITEPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSKM_ARATH
AccessionPrimary (citable) accession number: Q43725
Secondary accession number(s): Q8L4A2, Q9M1Z8, Q9M440
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 16, 2004
Last modified: November 3, 2009
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents