Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q43644

- NDUS1_SOLTU

UniProt

Q43644 - NDUS1_SOLTU

Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial

Gene
N/A
Organism
Solanum tuberosum (Potato)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.By similarity
    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi100 – 1001Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi111 – 1111Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi114 – 1141Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi128 – 1281Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi160 – 1601Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
    Metal bindingi164 – 1641Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi167 – 1671Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi173 – 1731Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi212 – 2121Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi215 – 2151Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi218 – 2181Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi262 – 2621Iron-sulfur 3 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. electron carrier activity Source: InterPro
    4. metal ion binding Source: UniProtKB-KW
    5. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    GO - Biological processi

    1. ATP synthesis coupled electron transport Source: InterPro
    2. response to oxidative stress Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    76 kDa mitochondrial complex I subunit
    Complex I-76kD
    Short name:
    CI-76kD
    NADH-ubiquinone oxidoreductase 76 kDa subunit
    OrganismiSolanum tuberosum (Potato)
    Taxonomic identifieri4113 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
    ProteomesiUP000011115: Unplaced

    Subcellular locationi

    Mitochondrion inner membrane By similarity
    Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity

    GO - Cellular componenti

    1. chloroplast Source: EnsemblPlants/Gramene
    2. mitochondrial respiratory chain complex I Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2727MitochondrionBy similarityAdd
    BLAST
    Chaini28 – 738711NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrialPRO_0000019973Add
    BLAST

    Proteomic databases

    PRIDEiQ43644.
    ProMEXiQ43644.

    Interactioni

    Subunit structurei

    Complex I is composed of about 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ43644. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ43644.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini66 – 144792Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini281 – 337574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 75 kDa subunit family.Curated
    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK03934.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    IPR015405. NuoG_C.
    [Graphical view]
    PfamiPF09326. DUF1982. 1 hit.
    PF00111. Fer2. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF54292. SSF54292. 1 hit.
    TIGRFAMsiTIGR01973. NuoG. 1 hit.
    PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q43644-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLGLLASRA LRSSRIIRNS TRTIVSTPEL KNADAAAAAA AADAPSDLPK    50
    RHPVGGARVH LPNPEDVIEV FVDGYPVKIP KGMTVLQACE IAGVDIPRFC 100
    YHSRLSIAGN CRMCLVEVEK SPKPVASCAM PALPGMKIKT DTPIAKKARE 150
    GVMEFLLMNH PLDCPICDQG GECDLQDQSM AFGSDRGRFT EMKRSVVDKN 200
    LGPLVKTVMT RCIQCTRCVR FASEVAGVED LGMLGRGSGE EIGTYVEKLM 250
    TSELSGNVID ICPVGALTSK PFAFKARNWE LKGTESIDVT DAVGSNIRID 300
    SRGPEVMRVV PRLNEDINEE WISDKTRFFY DGLKRQRLND PMIRGADGRF 350
    QAVSWRDALA IVAEVMHQIK PEEIVGVAGK LSDAESMMAL KDLLNKMGSN 400
    NIFCEGNGMH PNADLRSGYI MNTSISGLEK ADAFLLVGTQ PRVEAAMVNA 450
    RIHKTVKATN AKVGYVGPAA DFNYDHEHLG TDPQTLVEIA EGRHPFSSAL 500
    KNAKNPVIIV GAGVFDRDDK DAVFAAVDTI AKNNNVVRPD WNGLNVLLLN 550
    AAQVAALDLG LVPESDKCIE SAKFVYLMGA DDVNLDKLPD DAFVVYQGHH 600
    GDRGVYRANV ILPASAFTEK EGIYENTEGC AQITLPAVPT VGDARDDWKI 650
    VRALSEVAGV GLPYDSLGAI RSRIKTVAPN LLEVDERQPA TFSTSLRPEV 700
    SQKVSATPFT PAVENFYMTD AITRASKIMA QCSALLKK 738
    Length:738
    Mass (Da):79,970
    Last modified:November 1, 1996 - v1
    Checksum:i4AA955A62EEAD2E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85808 mRNA. Translation: CAA59818.1.
    PIRiS52737.
    RefSeqiNP_001275317.1. NM_001288388.1.
    UniGeneiStu.2837.

    Genome annotation databases

    GeneIDi102594243.
    KEGGisot:102594243.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85808 mRNA. Translation: CAA59818.1 .
    PIRi S52737.
    RefSeqi NP_001275317.1. NM_001288388.1.
    UniGenei Stu.2837.

    3D structure databases

    ProteinModelPortali Q43644.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q43644. 1 interaction.

    Proteomic databases

    PRIDEi Q43644.
    ProMEXi Q43644.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 102594243.
    KEGGi sot:102594243.

    Phylogenomic databases

    KOi K03934.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    IPR015405. NuoG_C.
    [Graphical view ]
    Pfami PF09326. DUF1982. 1 hit.
    PF00111. Fer2. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54292. SSF54292. 1 hit.
    TIGRFAMsi TIGR01973. NuoG. 1 hit.
    PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterisation of the 76 kDa iron-sulphur protein subunit of potato mitochondrial complex I."
      Rasmusson A.G., Heiser V., Irrgang K.D., Brennicke A., Grohmann L.
      Plant Cell Physiol. 39:373-381(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Desiree.
      Tissue: Leaf.

    Entry informationi

    Entry nameiNDUS1_SOLTU
    AccessioniPrimary (citable) accession number: Q43644
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3