Reviewed, UniProtKB/Swiss-Prot Q43644 (NDUS1_SOLTU)

Names and origin

Protein names	Recommended name:     NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial     EC=1.6.5.3     EC=1.6.99.3 Alternative name(s):     76 kDa mitochondrial complex I subunit     Complex I-75 kDa       Short name=CI-75kD
Organism	Solanum tuberosum (Potato)
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of about 30 different subunits By similarity.
Subcellular location	Mitochondrion inner membrane By similarity. Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion By similarity
	Chain	28 – 738	711	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial		PRO_0000019973
Regions
	Domain	66 – 144	79	2Fe-2S ferredoxin-type
Sites
	Metal binding	100	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	111	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	114	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	128	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	160	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	164	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	167	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	173	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	212	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	215	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	218	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	262	1	Iron-sulfur 3 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43644-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4AA955A62EEAD2E8	FASTA	738	79,970
	10 20 30 40 50 60 MGLGLLASRA LRSSRIIRNS TRTIVSTPEL KNADAAAAAA AADAPSDLPK RHPVGGARVH 70 80 90 100 110 120 LPNPEDVIEV FVDGYPVKIP KGMTVLQACE IAGVDIPRFC YHSRLSIAGN CRMCLVEVEK 130 140 150 160 170 180 SPKPVASCAM PALPGMKIKT DTPIAKKARE GVMEFLLMNH PLDCPICDQG GECDLQDQSM 190 200 210 220 230 240 AFGSDRGRFT EMKRSVVDKN LGPLVKTVMT RCIQCTRCVR FASEVAGVED LGMLGRGSGE 250 260 270 280 290 300 EIGTYVEKLM TSELSGNVID ICPVGALTSK PFAFKARNWE LKGTESIDVT DAVGSNIRID 310 320 330 340 350 360 SRGPEVMRVV PRLNEDINEE WISDKTRFFY DGLKRQRLND PMIRGADGRF QAVSWRDALA 370 380 390 400 410 420 IVAEVMHQIK PEEIVGVAGK LSDAESMMAL KDLLNKMGSN NIFCEGNGMH PNADLRSGYI 430 440 450 460 470 480 MNTSISGLEK ADAFLLVGTQ PRVEAAMVNA RIHKTVKATN AKVGYVGPAA DFNYDHEHLG 490 500 510 520 530 540 TDPQTLVEIA EGRHPFSSAL KNAKNPVIIV GAGVFDRDDK DAVFAAVDTI AKNNNVVRPD 550 560 570 580 590 600 WNGLNVLLLN AAQVAALDLG LVPESDKCIE SAKFVYLMGA DDVNLDKLPD DAFVVYQGHH 610 620 630 640 650 660 GDRGVYRANV ILPASAFTEK EGIYENTEGC AQITLPAVPT VGDARDDWKI VRALSEVAGV 670 680 690 700 710 720 GLPYDSLGAI RSRIKTVAPN LLEVDERQPA TFSTSLRPEV SQKVSATPFT PAVENFYMTD 730 AITRASKIMA QCSALLKK

References

[1]

"Molecular characterisation of the 76 kDa iron-sulphur protein subunit of potato mitochondrial complex I." Rasmusson A.G., Heiser V., Irrgang K.D., Brennicke A., Grohmann L. Plant Cell Physiol. 39:373-381(1998) [PubMed: 9615461] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Desiree. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X85808 mRNA. Translation: CAA59818.1.
PIR	S52737.
3D structure databases
SMR	Q43644. Positions 68-733.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q43644. 1 interaction.
Enzyme and pathway databases
BRENDA	1.6.5.3. 296. 1.6.99.3. 296.
Family and domain databases
InterPro	IPR001041. Ferredoxin. IPR006656. Mopterin_OxRdtase. IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. IPR015405. NuoG_C. [Graphical view]
Pfam	PF09326. DUF1982. 1 hit. PF00111. Fer2. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01973. NuoG. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS1_SOLTU

Accession

Primary (citable) accession number: Q43644

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)