NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial precursor

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Q43644 (NDUS1_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names	Recommended name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial EC=1.6.5.3 EC=1.6.99.3 Alternative name(s): 76 kDa mitochondrial complex I subunit Complex I-76kD Short name=CI-76kD NADH-ubiquinone oxidoreductase 76 kDa subunit
Organism	Solanum tuberosum (Potato) [Reference proteome]
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of about 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion inner membrane By similarity. Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 27

Mitochondrion By similarity

Chain

28 – 738

711

NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial

PRO_0000019973

Regions

Domain

66 – 144

2Fe-2S ferredoxin-type

Sites

Metal binding

100

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

111

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

114

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

128

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

160

Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity

Metal binding

164

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

167

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

173

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

212

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

215

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

218

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

262

Iron-sulfur 3 (4Fe-4S) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q43644 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4AA955A62EEAD2E8
Show »

FASTA

738

79,970

        10         20         30         40         50         60 
MGLGLLASRA LRSSRIIRNS TRTIVSTPEL KNADAAAAAA AADAPSDLPK RHPVGGARVH 

        70         80         90        100        110        120 
LPNPEDVIEV FVDGYPVKIP KGMTVLQACE IAGVDIPRFC YHSRLSIAGN CRMCLVEVEK 

       130        140        150        160        170        180 
SPKPVASCAM PALPGMKIKT DTPIAKKARE GVMEFLLMNH PLDCPICDQG GECDLQDQSM 

       190        200        210        220        230        240 
AFGSDRGRFT EMKRSVVDKN LGPLVKTVMT RCIQCTRCVR FASEVAGVED LGMLGRGSGE 

       250        260        270        280        290        300 
EIGTYVEKLM TSELSGNVID ICPVGALTSK PFAFKARNWE LKGTESIDVT DAVGSNIRID 

       310        320        330        340        350        360 
SRGPEVMRVV PRLNEDINEE WISDKTRFFY DGLKRQRLND PMIRGADGRF QAVSWRDALA 

       370        380        390        400        410        420 
IVAEVMHQIK PEEIVGVAGK LSDAESMMAL KDLLNKMGSN NIFCEGNGMH PNADLRSGYI 

       430        440        450        460        470        480 
MNTSISGLEK ADAFLLVGTQ PRVEAAMVNA RIHKTVKATN AKVGYVGPAA DFNYDHEHLG 

       490        500        510        520        530        540 
TDPQTLVEIA EGRHPFSSAL KNAKNPVIIV GAGVFDRDDK DAVFAAVDTI AKNNNVVRPD 

       550        560        570        580        590        600 
WNGLNVLLLN AAQVAALDLG LVPESDKCIE SAKFVYLMGA DDVNLDKLPD DAFVVYQGHH 

       610        620        630        640        650        660 
GDRGVYRANV ILPASAFTEK EGIYENTEGC AQITLPAVPT VGDARDDWKI VRALSEVAGV 

       670        680        690        700        710        720 
GLPYDSLGAI RSRIKTVAPN LLEVDERQPA TFSTSLRPEV SQKVSATPFT PAVENFYMTD 

       730 
AITRASKIMA QCSALLKK

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References

[1]

"Molecular characterisation of the 76 kDa iron-sulphur protein subunit of potato mitochondrial complex I."
Rasmusson A.G., Heiser V., Irrgang K.D., Brennicke A., Grohmann L.
Plant Cell Physiol. 39:373-381(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Desiree.
Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X85808 mRNA. Translation: CAA59818.1.
PIR	S52737.
3D structure databases
ProteinModelPortal	Q43644.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q43644. 1 interaction.
Proteomic databases
ProMEX	Q43644.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR001041. 2Fe-2S_ferredoxin-type. IPR012675. Beta-grasp_dom. IPR006656. Mopterin_OxRdtase. IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. IPR015405. NuoG_C. [Graphical view]
Pfam	PF09326. DUF1982. 1 hit. PF00111. Fer2. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
SMART	SM00929. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
SUPFAM	SSF54292. Ferredoxin. 1 hit.
TIGRFAMs	TIGR01973. NuoG. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS1_SOLTU

Accession

Primary (citable) accession number: Q43644

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Family and domain databases

Entry information

Relevant documents