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Q43644

- NDUS1_SOLTU

UniProt

Q43644 - NDUS1_SOLTU

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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial

Gene
N/A
Organism
Solanum tuberosum (Potato)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Binds 1 2Fe-2S cluster per subunit.By similarity
Binds 2 4Fe-4S clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi100 – 1001Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi111 – 1111Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi114 – 1141Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi128 – 1281Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi160 – 1601Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
Metal bindingi164 – 1641Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi167 – 1671Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi173 – 1731Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi212 – 2121Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi215 – 2151Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi218 – 2181Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi262 – 2621Iron-sulfur 3 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. electron carrier activity Source: EnsemblPlants/Gramene
  4. metal ion binding Source: UniProtKB-KW
  5. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: EnsemblPlants/Gramene
  2. gravitropism Source: EnsemblPlants/Gramene
  3. photorespiration Source: EnsemblPlants/Gramene
  4. proteasome core complex assembly Source: EnsemblPlants/Gramene
  5. response to misfolded protein Source: EnsemblPlants/Gramene
  6. response to oxidative stress Source: EnsemblPlants/Gramene
  7. ubiquitin-dependent protein catabolic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
76 kDa mitochondrial complex I subunit
Complex I-76kD
Short name:
CI-76kD
NADH-ubiquinone oxidoreductase 76 kDa subunit
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
ProteomesiUP000011115: Unplaced

Subcellular locationi

Mitochondrion inner membrane By similarity
Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity

GO - Cellular componenti

  1. chloroplast Source: EnsemblPlants/Gramene
  2. mitochondrial respiratory chain complex I Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727MitochondrionBy similarityAdd
BLAST
Chaini28 – 738711NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrialPRO_0000019973Add
BLAST

Proteomic databases

PRIDEiQ43644.
ProMEXiQ43644.

Interactioni

Subunit structurei

Complex I is composed of about 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme (By similarity).By similarity

Protein-protein interaction databases

IntActiQ43644. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ43644.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 144792Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini281 – 337574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated
Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK03934.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
[Graphical view]
PfamiPF09326. DUF1982. 1 hit.
PF00111. Fer2. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01973. NuoG. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43644-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLGLLASRA LRSSRIIRNS TRTIVSTPEL KNADAAAAAA AADAPSDLPK
60 70 80 90 100
RHPVGGARVH LPNPEDVIEV FVDGYPVKIP KGMTVLQACE IAGVDIPRFC
110 120 130 140 150
YHSRLSIAGN CRMCLVEVEK SPKPVASCAM PALPGMKIKT DTPIAKKARE
160 170 180 190 200
GVMEFLLMNH PLDCPICDQG GECDLQDQSM AFGSDRGRFT EMKRSVVDKN
210 220 230 240 250
LGPLVKTVMT RCIQCTRCVR FASEVAGVED LGMLGRGSGE EIGTYVEKLM
260 270 280 290 300
TSELSGNVID ICPVGALTSK PFAFKARNWE LKGTESIDVT DAVGSNIRID
310 320 330 340 350
SRGPEVMRVV PRLNEDINEE WISDKTRFFY DGLKRQRLND PMIRGADGRF
360 370 380 390 400
QAVSWRDALA IVAEVMHQIK PEEIVGVAGK LSDAESMMAL KDLLNKMGSN
410 420 430 440 450
NIFCEGNGMH PNADLRSGYI MNTSISGLEK ADAFLLVGTQ PRVEAAMVNA
460 470 480 490 500
RIHKTVKATN AKVGYVGPAA DFNYDHEHLG TDPQTLVEIA EGRHPFSSAL
510 520 530 540 550
KNAKNPVIIV GAGVFDRDDK DAVFAAVDTI AKNNNVVRPD WNGLNVLLLN
560 570 580 590 600
AAQVAALDLG LVPESDKCIE SAKFVYLMGA DDVNLDKLPD DAFVVYQGHH
610 620 630 640 650
GDRGVYRANV ILPASAFTEK EGIYENTEGC AQITLPAVPT VGDARDDWKI
660 670 680 690 700
VRALSEVAGV GLPYDSLGAI RSRIKTVAPN LLEVDERQPA TFSTSLRPEV
710 720 730
SQKVSATPFT PAVENFYMTD AITRASKIMA QCSALLKK
Length:738
Mass (Da):79,970
Last modified:November 1, 1996 - v1
Checksum:i4AA955A62EEAD2E8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X85808 mRNA. Translation: CAA59818.1.
PIRiS52737.
RefSeqiNP_001275317.1. NM_001288388.1.
UniGeneiStu.2837.

Genome annotation databases

GeneIDi102594243.
KEGGisot:102594243.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X85808 mRNA. Translation: CAA59818.1 .
PIRi S52737.
RefSeqi NP_001275317.1. NM_001288388.1.
UniGenei Stu.2837.

3D structure databases

ProteinModelPortali Q43644.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q43644. 1 interaction.

Proteomic databases

PRIDEi Q43644.
ProMEXi Q43644.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 102594243.
KEGGi sot:102594243.

Phylogenomic databases

KOi K03934.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
[Graphical view ]
Pfami PF09326. DUF1982. 1 hit.
PF00111. Fer2. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SUPFAMi SSF54292. SSF54292. 1 hit.
TIGRFAMsi TIGR01973. NuoG. 1 hit.
PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterisation of the 76 kDa iron-sulphur protein subunit of potato mitochondrial complex I."
    Rasmusson A.G., Heiser V., Irrgang K.D., Brennicke A., Grohmann L.
    Plant Cell Physiol. 39:373-381(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Desiree.
    Tissue: Leaf.

Entry informationi

Entry nameiNDUS1_SOLTU
AccessioniPrimary (citable) accession number: Q43644
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3