Reviewed,
UniProtKB/Swiss-Prot Q43621 (GSHRC_PEA)
Last modified
November 25, 2008.
Version 58.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glutathione reductase, cytosolic Short name=GRase Short name=GR EC=1.8.1.7 Alternative name(s): GOR2 |
| Organism | Pisum sativum (Garden pea) |
| Taxonomic identifier | 3888 [NCBI] |
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum |
Protein attributes
| Sequence length | 498 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Maintains high levels of reduced glutathione in the cytosol. May play a role in the restoration of the post-stress redox state of the cytosolic glutathione pool. |
| Catalytic activity | 2 glutathione + NADP(+) = glutathione disulfide + NADPH. |
| Cofactor | Binds 1 FAD per subunit By similarity. |
| Subcellular location | |
| Induction | Increased expression in the recovery (post-stress) phases of both drought and chilling. |
| Miscellaneous | The active site is a redox-active disulfide bond. |
| Sequence similarities | Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Domain | Redox-active center |
| Ligand | FAD Flavoprotein NADP |
| Molecular function | Oxidoreductase |
Gene Ontology (GO) | |
| Biological process | cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic processInferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: InterPro |
| Molecular function | FAD binding Inferred from electronic annotation. Source: InterPro NADP bindingInferred from electronic annotation. Source: InterPro electron carrier activityInferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 498 | 498 | Glutathione reductase, cytosolic | PRO_0000067964 | |||||||
Regions | |||||||||||
| Nucleotide binding | 63 – 72 | 10 | FAD By similarity | ||||||||
Sites | |||||||||||
| Active site | 471 | 1 | Proton acceptor By similarity | ||||||||
| Binding site | 233 | 1 | NADP By similarity | ||||||||
| Binding site | 239 | 1 | NADP By similarity | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 72 ↔ 77 | Redox-active By similarity | |||||||||
Sequences
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References
| [1] | "Cloning and characterisation of a cytosolic glutathione reductase cDNA from pea (Pisum sativum L.) and its expression in response to stress." Stevens R.G., Greissen G.P., Mullineaux P.M. Plant Mol. Biol. 35:641-654(1997) [PubMed: 9349285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Birte. Tissue: Leaf. |
Cross-references
Sequence databases | |
|---|---|
| X98274 mRNA. Translation: CAA66924.1. | |
| PIR | T06442. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1ONF based on UniProtKB Q94655. |
| ModBase | Search... |
Family and domain databases | |
| InterPro | IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006324. Glut_reduct_pln. IPR000815. Hg_reductase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR001864. Trypnth_redctse. [Graphical view] |
| Gene3D | G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit. |
| Pfam | PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view] |
| PRINTS | PR00368. FADPNR. PR00945. HGRDTASE. PR00411. PNDRDTASEI. PR00470. TRYPANRDTASE. |
| ProDom | PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR01424. gluta_reduc_2. 1 hit. |
| PROSITE | PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GSHRC_PEA | ||||||||
| Accession | Primary (citable) accession number: Q43621 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||

Clusters with


