Reviewed, UniProtKB/Swiss-Prot Q43621 (GSHRC_PEA)

Names and origin

Protein names	Recommended name:     Glutathione reductase, cytosolic       Short name=GRase       Short name=GR     EC=1.8.1.7 Alternative name(s):     GOR2
Organism	Pisum sativum (Garden pea)
Taxonomic identifier	3888 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol. May play a role in the restoration of the post-stress redox state of the cytosolic glutathione pool.
Catalytic activity	2 glutathione + NADP(+) = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Cytoplasm.
Induction	Increased expression in the recovery (post-stress) phases of both drought and chilling.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Glutathione reductase, cytosolic		PRO_0000067964
Regions
	Nucleotide binding	63 – 72	10	FAD By similarity
Sites
	Active site	471	1	Proton acceptor By similarity
	Binding site	233	1	NADP By similarity
	Binding site	239	1	NADP By similarity
Amino acid modifications
	Disulfide bond	72 ↔ 77		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43621-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 19AD08959764C6D0	FASTA	498	53,897
	10 20 30 40 50 60 MARKMLNDGE PDLKKGEEQG KVYDFDLFII GAGSGGVRAA RFSSNFGAKV GICELPFHPI 70 80 90 100 110 120 SSETIGGVGG TCVIRGCVPK KILVYGASYG GELQDARNFG WELNENVDFN WKKLLQKKTD 130 140 150 160 170 180 EINRLNGIYK RLLSNAGVKL FEGEGKIASP NEVEVTQLDG TKLSYSAKHI LIATGSRAQR 190 200 210 220 230 240 PNIPGQELGI TSDEALSLEE FPKRAVILGG GYIAVEFASI WRGMGSSVNL VFRKELPLRG 250 260 270 280 290 300 FDDEMRAVVA RNLEGRGINL HPRTNLAQLI KTEDGIKVIT DHGEELIADV VLFATGRSPN 310 320 330 340 350 360 SKRLNLEKVG VEFDKAGAIV VDEYSRTNIP SIWAVGDVTN RLNLTPVALM EASLFAKTVF 370 380 390 400 410 420 GGQASKPDYN DIPYAVFCIP PLSVVGLSEE QAVEQTKGDV LIFTSTFNPM KNTISGRQEK 430 440 450 460 470 480 TVMKLVVDAQ TDKVLGASMC GPDAPEIVQG IAIAIKCGAT KAQFDSTVGI HPSSAEEFVT 490 MRSETRRVTG GVKPKTNL

References

[1]

"Cloning and characterisation of a cytosolic glutathione reductase cDNA from pea (Pisum sativum L.) and its expression in response to stress." Stevens R.G., Greissen G.P., Mullineaux P.M. Plant Mol. Biol. 35:641-654(1997) [PubMed: 9349285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Birte. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X98274 mRNA. Translation: CAA66924.1.
PIR	T06442.
3D structure databases
HSSP	HSSP built from PDB template 1ONF based on UniProtKB Q94655.
ModBase	Search...
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006324. Glut_reduct_pln. IPR000815. Hg_reductase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR001864. Trypnth_redctse. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE. PR00411. PNDRDTASEI. PR00470. TRYPANRDTASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01424. gluta_reduc_2. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHRC_PEA

Accession

Primary (citable) accession number: Q43621

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)