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Protein

Glutathione reductase, cytosolic

Gene
N/A
Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Maintains high levels of reduced glutathione in the cytosol. May play a role in the restoration of the post-stress redox state of the cytosolic glutathione pool.

Miscellaneous

The active site is a redox-active disulfide bond.

Catalytic activityi

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei233NADPBy similarity1
Binding sitei239NADPBy similarity1
Active sitei471Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi63 – 72FADBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione reductase, cytosolic (EC:1.8.1.7)
Short name:
GR
Short name:
GRase
Alternative name(s):
GOR2
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000679641 – 498Glutathione reductase, cytosolicAdd BLAST498

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi72 ↔ 77Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ43621.

Expressioni

Inductioni

Increased expression in the recovery (post-stress) phases of both drought and chilling.

Structurei

3D structure databases

ProteinModelPortaliQ43621.
SMRiQ43621.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR006324. Glut-diS_reduct.
IPR001100. Pyr_nuc-diS_OxRdtase.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR012999. Pyr_OxRdtase_I_AS.
PfamiView protein in Pfam
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
PIRSFiPIRSF000350. Mercury_reductase_MerA. 1 hit.
SUPFAMiSSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.
TIGRFAMsiTIGR01424. gluta_reduc_2. 1 hit.
PROSITEiView protein in PROSITE
PS00076. PYRIDINE_REDOX_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q43621-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKMLNDGE PDLKKGEEQG KVYDFDLFII GAGSGGVRAA RFSSNFGAKV
60 70 80 90 100
GICELPFHPI SSETIGGVGG TCVIRGCVPK KILVYGASYG GELQDARNFG
110 120 130 140 150
WELNENVDFN WKKLLQKKTD EINRLNGIYK RLLSNAGVKL FEGEGKIASP
160 170 180 190 200
NEVEVTQLDG TKLSYSAKHI LIATGSRAQR PNIPGQELGI TSDEALSLEE
210 220 230 240 250
FPKRAVILGG GYIAVEFASI WRGMGSSVNL VFRKELPLRG FDDEMRAVVA
260 270 280 290 300
RNLEGRGINL HPRTNLAQLI KTEDGIKVIT DHGEELIADV VLFATGRSPN
310 320 330 340 350
SKRLNLEKVG VEFDKAGAIV VDEYSRTNIP SIWAVGDVTN RLNLTPVALM
360 370 380 390 400
EASLFAKTVF GGQASKPDYN DIPYAVFCIP PLSVVGLSEE QAVEQTKGDV
410 420 430 440 450
LIFTSTFNPM KNTISGRQEK TVMKLVVDAQ TDKVLGASMC GPDAPEIVQG
460 470 480 490
IAIAIKCGAT KAQFDSTVGI HPSSAEEFVT MRSETRRVTG GVKPKTNL
Length:498
Mass (Da):53,897
Last modified:November 1, 1997 - v1
Checksum:i19AD08959764C6D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98274 mRNA. Translation: CAA66924.1.
PIRiT06442.

Similar proteinsi

Entry informationi

Entry nameiGSHRC_PEA
AccessioniPrimary (citable) accession number: Q43621
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 27, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families