ID   COMT1_PRUDU             Reviewed;         365 AA.
AC   Q43609;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   20-JAN-2009, entry version 50.
DE   RecName: Full=Caffeic acid 3-O-methyltransferase;
DE            EC=2.1.1.68;
DE   AltName: Full=S-adenosysl-L-methionine:caffeic acid 3-O-methyltransferase;
DE            Short=CAOMT;
DE            Short=COMT;
GN   Name=COMT1;
OS   Prunus dulcis (Almond) (Prunus amygdalus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Rosales; Rosaceae; Amygdaloideae; Prunus.
OX   NCBI_TaxID=3755;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Texas; TISSUE=Root;
RA   Garcia-Mas J., Messeguer R., Arus P., Puigdomenech P.;
RT   "The caffeic acid O-methyltransferase from Prunus amygdalus.";
RL   (er) Plant Gene Register PGR95-006.
CC   -!- FUNCTION: Catalyzes the conversion of caffeic acid to ferulic acid
CC       and of 5-hydroxyferulic acid to sinapic acid. The resulting
CC       products may subsequently be converted to the corresponding
CC       alcohols that are incorporated into lignins.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + 3,4-dihydroxy-trans-
CC       cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-
CC       cinnamate.
CC   -!- PATHWAY: Aromatic compound metabolism; phenylpropanoid
CC       biosynthesis.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. Type 2
CC       family. COMT subfamily.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; X83217; CAA58218.1; -; mRNA.
DR   HSSP; P28002; 1KYZ.
DR   SMR; Q43609; 5-364.
DR   BRENDA; 2.1.1.68; 97707.
DR   GO; GO:0047763; F:caffeate O-methyltransferase activity; IEA:EC.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0009809; P:lignin biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR016461; O-MeTrfase_COMT_euk.
DR   InterPro; IPR001077; O_MeTrfase_2.
DR   InterPro; IPR012967; Plant_MeTrfase_dimerisation.
DR   InterPro; IPR011991; Wing_hlx_DNA_bd.
DR   Gene3D; G3DSA:1.10.10.10; Wing_hlx_DNA_bd; 1.
DR   Pfam; PF08100; Dimerisation; 1.
DR   Pfam; PF00891; Methyltransf_2; 1.
DR   PIRSF; PIRSF005739; O-mtase; 1.
PE   2: Evidence at transcript level;
KW   Lignin biosynthesis; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN         1    365       Caffeic acid 3-O-methyltransferase.
FT                                /FTId=PRO_0000063211.
FT   REGION      130    136       Substrate binding (By similarity).
FT   REGION      162    180       Substrate binding (By similarity).
FT   ACT_SITE    269    269       Proton acceptor (By similarity).
FT   BINDING     208    208       S-adenosyl-L-methionine; via carbonyl
FT                                oxygen (By similarity).
FT   BINDING     231    231       S-adenosyl-L-methionine (By similarity).
FT   BINDING     251    251       S-adenosyl-L-methionine (By similarity).
FT   BINDING     252    252       S-adenosyl-L-methionine; via amide
FT                                nitrogen (By similarity).
FT   BINDING     265    265       S-adenosyl-L-methionine (By similarity).
SQ   SEQUENCE   365 AA;  39762 MW;  C8C4BFEE7E0180E7 CRC64;
     MGSTGETQMT PTQVSDEEAN LFAMQLASAS VLPMVLKAAI ELDLLEIMAK AGPGVFLSPT
     DIASQLPTKN PDAPVMLDRM LRLLASYSIL TYSLRTLADG KVERLYGLGP VCKFLTKNEE
     GVSIAPLCLM NQDKVLLESW YHLKDAVLEG GIPFNKAYGM TAFEYHGTDP RFNKVFNRGM
     ADHSTITMKK ILETYKGFEG LTSVVDVGGG TGAVLNMIVS KYPSIKGINF DLPHVIEDAP
     QYPGVEHVGG DMFVSVPKGD AIFMKWICHD WSDEHCLKFL KNCYAALPDN GKVILGECIL
     PVAPDSSLAT KGVVHIDVIM LAHNPGGKER TEQEFQALAK GAGFQGFNVA CSAFNTYVIE
     FLKKN
//