Reviewed, UniProtKB/Swiss-Prot Q43609 (COMT1_PRUDU)

Names and origin

Protein names

Recommended name:
    Caffeic acid 3-O-methyltransferase
    EC=2.1.1.68
Alternative name(s):
    S-adenosysl-L-methionine:caffeic acid 3-O-methyltransferase
      Short name=CAOMT
      Short name=COMT

Gene names

Name:

COMT1

Organism

Prunus dulcis (Almond) (Prunus amygdalus)

Taxonomic identifier

3755 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Rosales › Rosaceae › Amygdaloideae › Prunus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid. The resulting products may subsequently be converted to the corresponding alcohols that are incorporated into lignins.
Catalytic activity	S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate.
Pathway	Aromatic compound metabolism; phenylpropanoid biosynthesis.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. Type 2 family. COMT subfamily.

Ontologies

Keywords
Biological process	Lignin biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	lignin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	caffeate O-methyltransferase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Caffeic acid 3-O-methyltransferase		PRO_0000063211
Regions
	Region	130 – 136	7	Substrate binding By similarity
	Region	162 – 180	19	Substrate binding By similarity
Sites
	Active site	269	1	Proton acceptor By similarity
	Binding site	208	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	231	1	S-adenosyl-L-methionine By similarity
	Binding site	251	1	S-adenosyl-L-methionine By similarity
	Binding site	252	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	265	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43609-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C8C4BFEE7E0180E7	FASTA	365	39,762
	10 20 30 40 50 60 MGSTGETQMT PTQVSDEEAN LFAMQLASAS VLPMVLKAAI ELDLLEIMAK AGPGVFLSPT 70 80 90 100 110 120 DIASQLPTKN PDAPVMLDRM LRLLASYSIL TYSLRTLADG KVERLYGLGP VCKFLTKNEE 130 140 150 160 170 180 GVSIAPLCLM NQDKVLLESW YHLKDAVLEG GIPFNKAYGM TAFEYHGTDP RFNKVFNRGM 190 200 210 220 230 240 ADHSTITMKK ILETYKGFEG LTSVVDVGGG TGAVLNMIVS KYPSIKGINF DLPHVIEDAP 250 260 270 280 290 300 QYPGVEHVGG DMFVSVPKGD AIFMKWICHD WSDEHCLKFL KNCYAALPDN GKVILGECIL 310 320 330 340 350 360 PVAPDSSLAT KGVVHIDVIM LAHNPGGKER TEQEFQALAK GAGFQGFNVA CSAFNTYVIE FLKKN

References

[1]	"The caffeic acid O-methyltransferase from Prunus amygdalus." Garcia-Mas J., Messeguer R., Arus P., Puigdomenech P. Plant Gene Register PGR95-006 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Texas. Tissue: Root.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83217 mRNA. Translation: CAA58218.1.
3D structure databases
HSSP	HSSP built from PDB template 1KYZ based on UniProtKB P28002.
SMR	Q43609. Positions 5-364.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.68. 97707.
Family and domain databases
InterPro	IPR016461. O-MeTrfase_COMT_euk. IPR001077. O_MeTrfase_2. IPR012967. Plant_MeTrfase_dimerisation. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF08100. Dimerisation. 1 hit. PF00891. Methyltransf_2. 1 hit. [Graphical view]
PIRSF	PIRSF005739. O-mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COMT1_PRUDU

Accession

Primary (citable) accession number: Q43609

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 20, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)