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Protein

Monodehydroascorbate reductase

Gene

AFRR

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of monodehydroascorbate to ascorbate, oxidizing NADH in the process.

Catalytic activityi

NADH + 2 monodehydroascorbate = NAD+ + 2 ascorbate.1 Publication

Cofactori

FADUniRule annotationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 2418FADBy similarityAdd
BLAST
Nucleotide bindingi165 – 18218NADBy similarityAdd
BLAST
Nucleotide bindingi191 – 1955FADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Monodehydroascorbate reductase (EC:1.6.5.4)
Short name:
MDAR
Alternative name(s):
Ascorbate free radical reductase
Short name:
AFR reductase
Gene namesi
Name:AFRRImported
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)Imported
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi
  • UP000004994 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 433433Monodehydroascorbate reductasePRO_0000209142Add
BLAST

Proteomic databases

PaxDbiQ43497.
PRIDEiQ43497.
ProMEXiQ43497.

Expressioni

Tissue specificityi

Expressed in leaves, and to a lesser degree in stems, roots and all stages of fruit.1 Publication

Inductioni

By wounding.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi4081.Solyc09g009390.2.1.

Structurei

3D structure databases

ProteinModelPortaliQ43497.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG1336. Eukaryota.
COG0446. LUCA.
InParanoidiQ43497.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.

Sequencei

Sequence statusi: Complete.

Q43497-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKSFKYVI VGGGVSAGYA AREFAKQGVK PGELAIISKE AVAPYERPAL
60 70 80 90 100
SKAYLFPEGA ARLPGFHVCV GSGGERQLPE WYAEKGISLI LSTEIVKADL
110 120 130 140 150
ASKTLVSAAG ESFKYQTLVI ATGTTVLKLS DFGVQGADSK NIFYLREIDD
160 170 180 190 200
ADQLVEALKA KKNGKAVVVG GGYIGLELSA VLRLNNIEVN MVYPEPWCMP
210 220 230 240 250
RLFTEGIAAF YEGYYKNKGV NIIKGTVAVG FDTHPNGEVK EVKLKDGRVL
260 270 280 290 300
EADIVVVGVG ARPLTTLFKG QVEEEKGGIK TDAFFKTSVP DVYAVGDVAT
310 320 330 340 350
FPLKMYNEIR RVEHVDHSRK SAEQAVKAIF ASEQGKSVDE YDYLPYFYSR
360 370 380 390 400
AFDLSWQFYG DNVGETVLFG DADPNSATHK FGQYWIKDGK IVGAFLESGS
410 420 430
PEENKAIAKV AKVQPPATLD QLAQEGISFA SKI
Length:433
Mass (Da):47,036
Last modified:November 1, 1996 - v1
Checksum:i6E3375DB73A1391A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41345 Genomic DNA. Translation: AAC41654.1.
PIRiT06407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41345 Genomic DNA. Translation: AAC41654.1.
PIRiT06407.

3D structure databases

ProteinModelPortaliQ43497.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4081.Solyc09g009390.2.1.

Proteomic databases

PaxDbiQ43497.
PRIDEiQ43497.
ProMEXiQ43497.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG1336. Eukaryota.
COG0446. LUCA.
InParanoidiQ43497.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Ascorbate free radical reductase mRNA levels are induced by wounding."
    Grantz A.A., Brummell D.A., Bennett A.B.
    Plant Physiol. 108:411-418(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. CastlemartImported.
    Tissue: FruitImported.

Entry informationi

Entry nameiMDAR_SOLLC
AccessioniPrimary (citable) accession number: Q43497
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: November 1, 1996
Last modified: November 11, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.