Reviewed, UniProtKB/Swiss-Prot Q43497 (MDAR_SOLLC)

Names and origin

Protein names

Recommended name:
    Monodehydroascorbate reductase
      Short name=MDAR
    EC=1.6.5.4
Alternative name(s):
    Ascorbate free radical reductase
      Short name=AFR reductase

Gene names

Name:

AFRR

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the conversion of monodehydroascorbate to ascorbate, oxidizing NADH in the process.
Catalytic activity	NADH + 2 monodehydroascorbate = NAD(+) + 2 ascorbate.
Cofactor	FAD By similarity.
Subcellular location	CytoplasmProbable.
Tissue specificity	Expressed in leaves, and to a lesser degree in stems, roots and all stages of fruit.
Induction	By wounding.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro monodehydroascorbate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Monodehydroascorbate reductase		PRO_0000209142
Regions
	Nucleotide binding	7 – 24	18	FAD By similarity
	Nucleotide binding	165 – 182	18	NAD By similarity
	Nucleotide binding	191 – 195	5	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43497-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6E3375DB73A1391A	FASTA	433	47,036
	10 20 30 40 50 60 MAEKSFKYVI VGGGVSAGYA AREFAKQGVK PGELAIISKE AVAPYERPAL SKAYLFPEGA 70 80 90 100 110 120 ARLPGFHVCV GSGGERQLPE WYAEKGISLI LSTEIVKADL ASKTLVSAAG ESFKYQTLVI 130 140 150 160 170 180 ATGTTVLKLS DFGVQGADSK NIFYLREIDD ADQLVEALKA KKNGKAVVVG GGYIGLELSA 190 200 210 220 230 240 VLRLNNIEVN MVYPEPWCMP RLFTEGIAAF YEGYYKNKGV NIIKGTVAVG FDTHPNGEVK 250 260 270 280 290 300 EVKLKDGRVL EADIVVVGVG ARPLTTLFKG QVEEEKGGIK TDAFFKTSVP DVYAVGDVAT 310 320 330 340 350 360 FPLKMYNEIR RVEHVDHSRK SAEQAVKAIF ASEQGKSVDE YDYLPYFYSR AFDLSWQFYG 370 380 390 400 410 420 DNVGETVLFG DADPNSATHK FGQYWIKDGK IVGAFLESGS PEENKAIAKV AKVQPPATLD 430 QLAQEGISFA SKI

References

[1]

"Ascorbate free radical reductase mRNA levels are induced by wounding." Grantz A.A., Brummell D.A., Bennett A.B. Plant Physiol. 108:411-418(1995) [PubMed: 7784511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Castlemart. Tissue: Fruit.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L41345 Genomic DNA. Translation: AAC41654.1.
PIR	T06407.
3D structure databases
HSSP	HSSP built from PDB template 1ONF based on UniProtKB Q94655.
ModBase	Search...
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00411. PNDRDTASEI.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

MDAR_SOLLC

Accession

Primary (citable) accession number: Q43497

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)