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Protein

Defensin J1-1

Gene
N/A
Organism
Capsicum annuum (Bell pepper)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plant defense peptide with antifungal activity against F.oxysporum and B.cinerea.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Defensin J1-1
OrganismiCapsicum annuum (Bell pepper)
Taxonomic identifieri4072 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeCapsiceaeCapsicum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Chaini28 – 7548Defensin J1-1PRO_0000007036Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 74By similarity
Disulfide bondi41 ↔ 61By similarity
Disulfide bondi47 ↔ 68By similarity
Disulfide bondi51 ↔ 70By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed in orange and red ripe fruit and to a lesser extent in mature, green fruit. Present in trace in young, green fruit.

Developmental stagei

Transcripts remains very low during fruit development and dramatically increases during ripening.

Inductioni

By wounding.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliQ43413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DEFL family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008177. G_Purothionin.
IPR008176. Gamma-thionin.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PRINTSiPR00288. PUROTHIONIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43413-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGFSKVVAT IFLMMLLVFA TDMMAEAKIC EALSGNFKGL CLSSRDCGNV
60 70
CRREGFTDGS CIGFRLQCFC TKPCA
Length:75
Mass (Da):8,118
Last modified:November 1, 1996 - v1
Checksum:i6E2A8ECE7614A51B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95363 Genomic DNA. Translation: CAA64653.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95363 Genomic DNA. Translation: CAA64653.1.

3D structure databases

ProteinModelPortaliQ43413.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008177. G_Purothionin.
IPR008176. Gamma-thionin.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PRINTSiPR00288. PUROTHIONIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Fruit-specific expression of a defensin-type gene family in bell pepper. Upregulation during ripening and upon wounding."
    Meyer B., Houlne G., Pozueta-Romero J., Schantz M.L., Schantz R.
    Plant Physiol. 112:615-622(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-69, CHARACTERIZATION.
    Strain: cv. Yolo Wonder.
    Tissue: Fruit.

Entry informationi

Entry nameiDEF1_CAPAN
AccessioniPrimary (citable) accession number: Q43413
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1996
Last modified: September 16, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.