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Protein

Peroxidase 71

Gene

PER71

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei71 – 711Transition state stabilizerPROSITE-ProRule annotation
Active sitei75 – 751Proton acceptorPROSITE-ProRule annotation
Metal bindingi76 – 761Calcium 1PROSITE-ProRule annotation
Metal bindingi79 – 791Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi81 – 811Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi83 – 831Calcium 1PROSITE-ProRule annotation
Metal bindingi85 – 851Calcium 1PROSITE-ProRule annotation
Binding sitei167 – 1671Substrate; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi197 – 1971Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi198 – 1981Calcium 2PROSITE-ProRule annotation
Metal bindingi248 – 2481Calcium 2PROSITE-ProRule annotation
Metal bindingi251 – 2511Calcium 2PROSITE-ProRule annotation
Metal bindingi256 – 2561Calcium 2PROSITE-ProRule annotation

GO - Molecular functioni

  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • peroxidase activity Source: TAIR

GO - Biological processi

  • defense response to fungus Source: TAIR
  • hydrogen peroxide catabolic process Source: UniProtKB-KW
  • lignin metabolic process Source: TAIR
  • plant-type cell wall organization Source: GO_Central
  • respiratory burst Source: TAIR
  • response to oxidative stress Source: InterPro
  • rhythmic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Biological rhythms, Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G64120-MONOMER.

Protein family/group databases

PeroxiBasei237. AtPrx71.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase 71 (EC:1.11.1.7)
Short name:
Atperox P71
Alternative name(s):
ATP15a
ATPO2
Gene namesi
Name:PER71
Synonyms:P71
Ordered Locus Names:At5g64120
ORF Names:MHJ24.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G64120.

Subcellular locationi

  • Secreted PROSITE-ProRule annotation

GO - Cellular componenti

  • apoplast Source: TAIR
  • cell wall Source: TAIR
  • membrane Source: TAIR
  • plant-type cell wall Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 328305Peroxidase 71PRO_0000023736Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 120PROSITE-ProRule annotation
Disulfide bondi77 ↔ 82PROSITE-ProRule annotation
Disulfide bondi126 ↔ 324PROSITE-ProRule annotation
Disulfide bondi204 ↔ 235PROSITE-ProRule annotation
Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence analysis
Glycosylationi262 – 2621N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ43387.
PRIDEiQ43387.

Expressioni

Tissue specificityi

Slightly expressed in roots.

Inductioni

Induced in response of mechanical wounding. Induced by methyl jasmonate, a plant defense-related signaling molecule. Expressed under a diurnal rhythm (circadian clock control).3 Publications

Gene expression databases

GenevisibleiQ43387. AT.

Interactioni

Protein-protein interaction databases

BioGridi21775. 1 interaction.
STRINGi3702.AT5G64120.1.

Structurei

3D structure databases

ProteinModelPortaliQ43387.
SMRiQ43387. Positions 39-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J946. Eukaryota.
ENOG410YEXC. LUCA.
HOGENOMiHOG000237556.
InParanoidiQ43387.
KOiK00430.
OMAiPTIDPTF.
PhylomeDBiQ43387.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43387-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLVRSLCLL ITFLNCLIIS VHGQATARPG PVSGTRIGFY LTTCPRAETI
60 70 80 90 100
VRNAVNAGFS SDPRIAPGIL RMHFHDCFVQ GCDGSILISG ANTERTAGPN
110 120 130 140 150
LNLQGFEVID NAKTQLEAAC PGVVSCADIL ALAARDTVIL TQGTGWQVPT
160 170 180 190 200
GRRDGRVSLA SNANNLPGPR DSVAVQQQKF SALGLNTRDL VVLVGGHTIG
210 220 230 240 250
TAGCGVFRNR LFNTTGQTAD PTIDPTFLAQ LQTQCPQNGD GSVRVDLDTG
260 270 280 290 300
SGSTWDTSYY NNLSRGRGVL QSDQVLWTDP ATRPIVQQLM APRSTFNVEF
310 320
ARSMVRMSNI GVVTGANGEI RRVCSAVN
Length:328
Mass (Da):34,890
Last modified:November 1, 1996 - v1
Checksum:iD590434E203E86C7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181I → V in AAM64354 (Ref. 5) Curated
Sequence conflicti98 – 981G → S in AAM64354 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99097 mRNA. Translation: CAA67551.1.
AB008266 Genomic DNA. Translation: BAB10280.1.
CP002688 Genomic DNA. Translation: AED97842.1.
AF428274 mRNA. Translation: AAL16106.1.
BT002622 mRNA. Translation: AAO11538.1.
AY086282 mRNA. Translation: AAM64354.1.
AJ006961 Genomic DNA. Translation: CAA07353.1.
RefSeqiNP_201217.1. NM_125808.2.
UniGeneiAt.24476.
At.74979.

Genome annotation databases

EnsemblPlantsiAT5G64120.1; AT5G64120.1; AT5G64120.
GeneIDi836533.
GrameneiAT5G64120.1; AT5G64120.1; AT5G64120.
KEGGiath:AT5G64120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99097 mRNA. Translation: CAA67551.1.
AB008266 Genomic DNA. Translation: BAB10280.1.
CP002688 Genomic DNA. Translation: AED97842.1.
AF428274 mRNA. Translation: AAL16106.1.
BT002622 mRNA. Translation: AAO11538.1.
AY086282 mRNA. Translation: AAM64354.1.
AJ006961 Genomic DNA. Translation: CAA07353.1.
RefSeqiNP_201217.1. NM_125808.2.
UniGeneiAt.24476.
At.74979.

3D structure databases

ProteinModelPortaliQ43387.
SMRiQ43387. Positions 39-328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21775. 1 interaction.
STRINGi3702.AT5G64120.1.

Protein family/group databases

PeroxiBasei237. AtPrx71.

Proteomic databases

PaxDbiQ43387.
PRIDEiQ43387.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G64120.1; AT5G64120.1; AT5G64120.
GeneIDi836533.
GrameneiAT5G64120.1; AT5G64120.1; AT5G64120.
KEGGiath:AT5G64120.

Organism-specific databases

TAIRiAT5G64120.

Phylogenomic databases

eggNOGiENOG410J946. Eukaryota.
ENOG410YEXC. LUCA.
HOGENOMiHOG000237556.
InParanoidiQ43387.
KOiK00430.
OMAiPTIDPTF.
PhylomeDBiQ43387.

Enzyme and pathway databases

BioCyciARA:AT5G64120-MONOMER.

Miscellaneous databases

PROiQ43387.

Gene expression databases

GenevisibleiQ43387. AT.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "From expressed sequence tags to structure, function, evolution and expression of 28 ER-targeted Arabidopsis peroxidases."
    Welinder K.G., Jespersen H.M., Kjaersgaard I.V.H., Justesen A.F., Oestergaard L., Abelskov A.K., Jensen R.B., Hansen L.N., Rasmussen S.K.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Analysis of two incompletely spliced Arabidopsis cDNAs encoding novel types of peroxidase."
    Justesen A.F., Jespersen H.M., Welinder K.G.
    Biochim. Biophys. Acta 1443:149-154(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 49-201.
    Strain: cv. Columbia.
  7. "Computational analyses and annotations of the Arabidopsis peroxidase gene family."
    Oestergaard L., Pedersen A.G., Jespersen H.M., Brunak S., Welinder K.G.
    FEBS Lett. 433:98-102(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: cv. Columbia.
  8. "Orchestrated transcription of key pathways in Arabidopsis by the circadian clock."
    Harmer S.L., Hogenesch J.B., Straume M., Chang H.-S., Han B., Zhu T., Wang X., Kreps J.A., Kay S.A.
    Science 290:2110-2113(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: cv. Columbia.
  9. "Coordinated plant defense responses in Arabidopsis revealed by microarray analysis."
    Schenk P.M., Kazan K., Wilson I., Anderson J.P., Richmond T., Somerville S.C., Manners J.M.
    Proc. Natl. Acad. Sci. U.S.A. 97:11655-11660(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: cv. Columbia.
  10. "Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis."
    Cheong Y.H., Chang H.-S., Gupta R., Wang X., Zhu T., Luan S.
    Plant Physiol. 129:661-677(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: cv. Columbia.
  11. "Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana."
    Tognolli M., Penel C., Greppin H., Simon P.
    Gene 288:129-138(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.

Entry informationi

Entry nameiPER71_ARATH
AccessioniPrimary (citable) accession number: Q43387
Secondary accession number(s): Q9SBA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.